Protective effect of royal jelly on the sperm parameters and testosterone level and lipid peroxidation in adult mice treated with oxymetholone

Document Type : Original Research Article

Authors

1 Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, I. R. Iran

2 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, I. R. Iran

3 Department of Biochemistry, Faculty of Science, Urmia University, Urmia, I. R. Iran

Abstract

Objectives: The aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (MDA) production in mice.
Materials and Methods: Thirty-two adult male NMRI mice weighing 30±2 g were used. All the animals were divided into 4 groups. Control group: received saline 0.1 ml/mouse/day orally for 30 days. Royal Jelly group (RJ): received royal jelly at dose of 100 mg/kg daily for 30 days orally. Oxymetholone group: the received Oxymetholone (OX) at dose of 5 mg/kg daily for 30 days orally. Royal Jelly+Oxymetholone group: received royal jelly at dose of 100 mg/kg/day orally concomitant with OX administration.
Sperm count, sperm motility, viability, maturity, and DNA integrity were analyzed. Furthermore, serum testosterone and MDA concentrations were determined.
Results: In Oxymetholone group, sperm count, motility as well as testosterone concentration reduced significantly (p<0.05), while significant (p<0.05) increases in immature sperm, sperm with DNA damaged, and MDA concentration were announced in Oxymetholone group in comparison with control group and Royal Jelly+Oxymetholone group. RJ caused partially amelioration in all of the above- mentioned parameters in Royal Jelly+Oxymetholone group.
Conclusion: In conclusion, RJ may be used in combination with OX to improve OX-induced oxidative stress and male infertility.

Keywords

References

Ahmed T, Abdelhafiza B, Jehan A. 2008. Muhamad b Midcyclepericoitalintravaginal bee honey and royal jelly for male factor infertility. Int J Gynecol Obstet, 101: 146-149.
Aitken RJ. 1994. A free radical theory of male infertility. Reprod Fertil Dev, 6: 19-24.
Aitken RJ. 1995. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev, 7: 659–668.
Birkhead TR, Martinez, JG, Burke T, Froman, DP. 1999. Sperm mobility determines the outcome of sperm competition in the domestic fowl. Bio Sciences, 266: 1759-1764.
Bjorndahl L, Soderlund I, Kvist U. 2003.Consider replacing with Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitalityassessment Evaluation of the one-step nigrosin-eosin stainingtechnique for human sperm vitality assessment. Hum Reprod, 18: 813-816.
Bungum M, Bungum L, Giwercman A. 2011.Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. Asian J Androl, 13: 69-75.
Carrell DT, Emery, BR, Hammoud S. 2007. Altered protamine expression and diminished spermatogenesis. Hum Reprod, 13: 313-327.
Chang H, Suarez SS. 2011. Two distinct Ca2+ signaling pathways modulate sperm flagellar beating patterns in mice. Biol Reprod, 85: 296-305.
Christiansen K. 2001. Behavioural Effects of Androgen in Men and Women. J Endocr, 170: 39-48.
Collar ML, Hines M. 1995. Human behavioral sex differences: A role for gonadal hormones during early development. Psychol bull, 118: 55-107.
Comhaire FH, Christophe AS, Zalata AA, Dhooge WS, Mahmoud AM, Depudt CE. 2000. The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men. Prostaglandins Leukot Essent Fat Acids, 63: 159-165.
Dziuk PJ. 1996. Factors that influence the proportion of offspring sired by a male following heterospermic insemination. Anim Reprod Sci, 43: 65-88.
Esposito G, Jaiswal BS, Xie F, Krajnc-Franken, MA, Robben TJ, Strik AM, Kuil C, Philipsen RL, van Duin M, Conti M, Gossen JA. 2004. Mice deficient for soluble adenylyl cyclase are infertile because of a severe sperm-motility defect. Proc Natl Acad Sci, 101: 2993-2998.
Fraser L, Strzezek J. 2007. Effect of different procedures of ejaculate collection extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. Anim Reprod Sci, 99: 317-319.
Fujii A, Kobayashi S, Kuboyama N. 1990. Augmentation of wound healing by royal jelly in streptozotocin-diabetic rats. Jpn J Pharmacol, 53: 331-337.
Glander HJ, Kratzsch J,Weisbrich C, Birkenmeier G. 1996 .Insulin-like growth factor-I and alpha-2 macroglobulin in seminal plasma correlation with semen quality. Hum Reprod, 11: 746-750.
Gomez E, Irvine DS, Aitken RJ. 1998. Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4-hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function. Int J Androl, 21: 81-84.
Hafez B, Hafez ESE. 2000. Reproduction in Farm Animals, pp. 509, Lippincott, William & Wilkins.
Hashimoto M, Kanda M, Ikeno K, Hayashi Y, Nakamura T. 2005. Oral administration of royal jelly facilitates mrna expression of glial line-derived neurotrophic factor and neurofilament h in the hippocampus of the adult mouse brain. Biosci Biotechnol Biochem, 69: 800-805.
Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa Sh. 2007. Royal Jelly-induced neurite outgrowth from rat pheochromocytoma PC12 cell requires integrin signal independent of activation of extracellular signalregulated kinases. Biomed Res, 28: 139-146.
Haupt HA, Rovere GD. 1984.Anabolic steroids. A review of the literature.Am  J Sports Med, 12: 4469-4485.
Hayes K J. 1965. The so called 'levatorani' of the rat. Acta endocr, 48: 337.
Henricks  D.M, Kouba AJ, Lackey BR, Boone WR, Gray SL. 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol Reprod, 59: 330-337.
Hosseinzadeh H, sadeghnia RH. 2005. Safranal, aconstituent of corcussativus, attenuated cerebral ischemia induced oxidative damage in rat hippocampus .pharm pharmaceut, 8: 394-399.
Hunter FM, Birkhead TR. 2002.Sperm viability and sperm competition in insects. Current Biology, 12: 121-123.
Juris E, Janis B, Vika L, Jekaterina E, Janis Z. 2001. Comparative Study of Cytochemical Tests for Sperm Chromatin Integrity. J Androl, 22: 45-52.
Kanbur M, Eraslan G, Silici S, Karabacak M. 2009. Effects of sodium fluoride exposure on some biochemical parameters in mice: Evaluation of the ameliorative effect of royal jelly applications on these parameters. Food Chem Toxicol, 47: 1184-1189.
Kendall D. 1948. Thrombosis of intracranial veins. Brain, 71: 386-402.
Kincl FA, Macqueo M, Dorfman R. 1965. Influence of various steroids on testes and accessory sex organs in the rat. Actaendocr, 49: 145.
Klaiber  EL, Henzl  MR, Lloyd  CW, Segre  EJ. 1973. Corpus luteum inhibiting action of oxymetholone. J Clin Endocr, 36: 142-147.
Lejeune  H , Chuzel  F , Thomas  T, Avallet O, Habert R, Durand P, Saez J. 1996. Paracrine regulation of Leydig cells. Ann Endocrinol Paris, 57: 55-63.
Lishko PV, Botchkina IL, Kirichok Y. 2011. Progesterone activates the principal Ca2+ channel of human sperm. Nature, 471: 387-391.
Morris ID, Ilott S, Dixon L, Brison DR. 2002. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod, 17: 990-998.
Nagai T, Inoue R. 2004. Preparation and the functional properties of water extract and alkaline extract of royal jelly. Food Chem, 84:181-186.
Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hara H. 2009. Comparison of bee products based on assays of antioxidant capacities. Complement Alternm Med, 9: 1-4
Nolan MA, Babcock  DF, Wennemuth  G, Brown W, Burton  KA, McKnight  GS. 2004. Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility. Proc  Natl  Acad Sci, 101: 13483-13488.
Orlande F, Jezequel A, Melliti A. 1964. The action of some anabolic steroids on the structure and the function of human liver cell. Gastro-Enterol, 7: 109-113.
Overly WL, Dankoff JA, Wong BK, Sengh UD. 1984. Androgens and hepatocellulacarcinoma in athetes. Ann Intern Med, 100: 158-159.
Quill TA, Sugden SA, Rossi KL, Doolittle LK, Hammer RE, Garbers DL. 2003. Hyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Natl Acad  Sci, 100: 14869-14874.
Renard P, Grizard G, Griveau JF, Sion B, Boucher D, Le Lannou D. 1996. Improvement of motility and fertilization potential of postthaw human sperm using glutathione.Cryobiology, 33: 311-319.
SanchezPena LC, Reyes BE,LopezCarrillo L,Recio R,MoranMartinez J, Cebrian ME,QuintanillaVega B. 2004. Organophosphorouspesticide exposure alters sperm chromatin structure in Mexican agricultural workers. Toxicol Appl Pharm, 196: 108-113.
Sandifer SH, Wilkins R, Loadholt CB, Lane LG, ldridge JC. 1979. Spermatogenesis in agricultural workers exposed to dibromochloropropane (DBCP). Bull Environ Contam Toxicol, 23: 703-710.
Sanocka  D, Kurpisz  M. 2004. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol, 2: 12-19.
Shimoda M, Nakajin S, Oikawa T. 1978. Biochemical studies on vasodilative factor in royal jelly. J Pharm Soc Jpn, 98: 139-145.
Sver L, Orsolic N, Tadic Z. 1996. A royal jelly as a new potential immunomodulator in rats and mice. Comp Immunol Microbiol Infect Dis, 19: 31-38.
Takenaka T. 1982. Chemical composition of royal jelly. Honeybee Sci, 3: 69-74.
Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. 2008. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia, 40: 245-251.
Vannelli  BG, Barni  T, Orlando C, Natali A, Serio  M, Balboni GC. 1988. Insulin-like growth factor-I (IGF-I) and IGF-I receptor in human testis: an immunohistochemical study. Fertil Steril, 49: 666-669.
Vickers MH, Casey PJ, Champion ZJ, Gravance CG, Breier BH. 1999. IGFI treatment increases motility and improves morphology of immature spermatozoa in the GH-deficient dwarf (dw/dw) rat.GrowthHorm. IGF Res, 9: 236-270.
Whorton D, Krauss R, Marshall S, Milby T. 1977. Infertility in male pesticide workers. Lancetii, 1259-1261.
World Health Organization. laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 2010. pp. 26-45. Cambridge, United Kingdom.
Wyrobek AJ, Bruce WR. 1975. Chemicalinduction of sperm abnormalities in mice. Cell Biol, 72: 4425-4429.
Yang A, Zhou M, Zhang L, Xie G, Chen H, Liu Z, Ma W. 2012. Influence of royal jelly on the reproductive function of puberty male rats. Food Chem Toxicol, 50:1834-1840.
Zeng XH, Yang C, Kim ST, Lingle CJ, Xia XM. 2011. Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa. Proc Natl Acad Sci, 108: 5879-5884.
 
Avicenna Journal of Phytomedicine
Volume 4, Issue 1 - Serial Number 1
January and February 2014
Pages 43-52

Files

Share

How to cite

Statistics