Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory

Document Type : Original Research Article

Authors

1 School of Pharmaceutical Education & Research, Berhampur University, Bhanja Bihar, Berhampur-760007, Odisha, India

2 Department of Pharmacology, Roland Institute of Pharmaceutical Sciences, Berhampur-760010, Odisha, India

3 Department of Botany & Biotechnology, Khallikote Autonomous College, Berhampur-760001, Odisha, India

Abstract


Objective: The present study was carried out to investigate the neuropharmacological activities of ethyl acetate extract of Mimosa pudica (EAMP) leaves on anxiety, depression and memory in a mouse model.

Materials and Methods: Anti-anxiety potential of EAMP was evaluated by elevated plus maze (EPM), light-dark box (LDB) and social interaction (SI) tests in mice.Anti-depressant potential of EAMP was evaluated by forced swimming (FST), tail suspension (TST), and open field tests (OFT). The behavioral findings were further corroborated with estimation of neurotransmitters and their metabolites from mouse brain homogenate. Effect on learning and memory was evaluated by EPM, passive avoidance (PA) tests. Further, it was confirmed with assessment of acetylcholinesterase and caspase-3 activity in brain homogenate.

Results: EAMP showed significant anti-anxiety activity by increasing the time spent in open arm of EPM, light box of LDB. Social interaction time was increased significantly (p<0.01) as compared to vehicle control. There was also significant reduction of immobility time in both FST and TST without any changes in locomotor activity in the OFT. Monoamine neurotransmitters (dopamine and norepinephrine) concentrations were increased significantly (p<0.01) after 4 weeks of treatment as compared to stress control and substantiated the anti-depressant activity. Step down latency was increased (p<0.01) in PA test and transfer latency was decreased (p<0.01) in EPM test of EAMP-treated mice. Acetylcholinesterase and caspase-3 activity was significantly (p<0.05) changed in mice treated with EAMP (200 and 400 mg/kg).

Conclusion: The results revealed that EAMP has anti-anxiety, anti-depressant and memory enhancing activities that are mediated through multiple mechanisms.

Keywords

Main Subjects

References

Baby J, Jency G, Jeevitha M. 2013. Pharmacology and Traditional Uses of Mimosa pudica. Int J   Pharm Sci Drug Res, 5: 41-44.
Bhattamisra SK, Khannab VK, Agrawal AK, Singh PN, Singh SK. 2008. Antidepressant activity of standardized extract of Marsilea minuta Linn. J Ethnopharmacol, 117: 51-57.
Bhattamisra SK, Singh PN, Singh SK, Kumar V. 2007. Anxiolytic activity of Marsilea minuta Linn. J Herb Med Toxicol, 1: 15-20.
Bhattamisra SK, Singh PN, Singh SK. 2012. Effect of standardized extract of Marsilea minuta on learning and memory performance in rat amnesic models. Pharm Biol, 50: 766-772.
Bourin M, Chenu F, Ripoll N, David DJP. 2005. A proposal of decision tree to screen putative antidepressants using forced swim and tail suspension tests. Behav Brain Res, 164: 266-269.Chatterjee A, Prakash SC. 2000. The Treatise of Indian Medicinal Plants. Vol 2. pp. 65-66. Publications and Information Directorate, CSIR, New Delhi.
Crawley J, Goodwin FK. 1980. Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepines. Pharmacol Biochem Behav, 13:167-170.
Darreh-Shori T, Meurling L, Pettersson T, Hugosson K, Hellstrom-Lindahl E, Andreasen N, et al. 2006. Changes in the activity and protein levels of CSF acetylcholinesterases in relation to cognitive function of patients with mild Alzheimer’s disease following chronic donepezil treatment. J Neural Transm, 113:1791–801.
Dash PK, Blum S, Moore AN. 2000. Caspase activity plays an essential role in long-term memory. Neuroreport, 11:2811–2816.
Elisabetsky E, Costa-Campos L. 2006. The alkaloid alstonine: a review of its pharmacological properties. Evid-Based Comp Alt Med, 3: 39-48.
Farah IN, Taufik HM, Moklas MAM, Sharida F, Raudzaha NAR, Shamima AR, et al. 2011. Antidepressant-like effect of mitragynine isolated from Mitragyna speciosa Korth in mice model of depression. Phytomedicine, 18: 402-407.
Farooqui T, Farooqui AA. 2009. Aging: An important factor for the pathogenesis of neurodegenerative diseases. MechAgeing Dev, 130: 203-215.
File SE. 1996. The use of social interaction as a method for detecting anxiolytic activity of chlordiazepoxide like drugs. J Neurosci Meth, 2: 219-238.
Gireesh KS, Sudhir KC, Geeta R, Shyam SC, Vikas K. 2013. Potential antianxiety activity of Fumaria indica: A preclinical study. Pharmacogn Mag, 9(33): 14-22.
Grundmann O, Nakajima JI, Seo S, Butterweck V. 2007. Anti-anxiety effects of Apocynum venetum L. in the elevated plus maze test. J Ethnopharmacol, 110: 406-411.
Gulyaeva NV, Kudryashov IE, Kudryashova IV. 2003. Caspase activity is essential for long-term potentiation. J Neurosci Res, 73:853–864.
Gulyaeva NV. 2003. Non-apoptotic functions of caspase-3 in the nervous tissue. Biochemistry (Moscow), 68:1459–1470.
Hafsa A, Sakshi S, Anurag M, and Rajiv G. 2012. Mimosa pudica L. (Laajvanti): An overview. Pharmacogn Rev, 6: 115-124.
Haixia D, Ying C, Xinmin L, Qiong W, Liwei W, William J. 2009. Antidepressant effects of ginseng total saponins in the forced swimming test and chronic mild stress models of depression. Prog Neuropsychopharmacol Biol Psychiatry, 33: 1417-1424.
Jans L, Riedel WJ, Markus CR, Blokland A. 2007. Serotonergic vulnerability and depression: assumptions, experimental evidence and implications. Mol Psychiatry, 12: 522-543.
Joy PP, Thomas J, Mathew S, Skaria BP. 2001. Medicinal Plants. Trop Horticulture. 2:449-632.
Kim JH, Kim SY, Lee SY, Jang CG. 2007. Antidepressant-like effects of Albizzia julibrissin in mice: involvement of the 5-HT1A receptor system. Pharmacol Biochem Behav, 87: 41-47.Kulkarni SK. 1999. Handbook of Experimental Pharmacology, pp. 135-137. Vallabh Prakashan, Delhi.
Kumar D, Bhat ZA, Kumar V, Khan NA, Chashoo IA, Zargar MI. 2012. Effects of Stachys tibetica essential oil in anxiety. Eur J Integr Med, 4: e169–e176.
Lane RM, Potkin SG, Enz A. 2006. Targeting acetylcholinesterase and butyrylcholinesterase in dementia. Int J Neuropsycopharmacol, 9:101–124.
 
Lucian H, Jaures AN, Oana C, Monica H, Paula P, Marius M. 2015. Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer’s disease. Behav  Brain Funct, 11:13.
Machado DG, Bettio LE, Cunha MP, Capra JC, Dalmarco JB, Pizzolatti MG. 2009.  Antidepressant like effect of the extract of Rosmarinus officinalis in mice: involvement of the monoaminergic system. Prog Neuropsychopharmacol Biol Psychiatry, 33: 642-650.
Merlin FF, Narsimhan D. 2009. Plant names and uses as indicators of knowledge patterns. Indian J Trad Knowledge, 8: 645-648.
Mesulam M. 2004. The cholinergic lesion of Alzheimer’s disease: pivotal factor or side show? Learn Memory, 11: 43–49.
Nemeroff CB. 2007. The burden of severe depression: a review of diagnostic challenges and treatment alternatives. J Psychiatr Res, 41:189-206.
Papandreou MA, Tsachaki M, Efthimiopoulos S, Cordopatis P, Lamari FN, Margarity M. 2011. Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behav Brain Res, 219:197-204.
Poonam M and Shradha B. 2011. Antianxiety activity of Coriandrum sativum assessed using different experimental anxiety models. Indian J Pharmacol, 43: 574-577.
Porsolt RD, Bertin A, Jalfre M. 1977. Behavioural despair in mice: a primary screening test for antidepressants. Psychopharmacol (Berl), 229: 327-336.
Rupniak NM, Tye SJ, Field MJ. 1997. Enhanced performance of spatial and visual recognition memory tasks by the selective acetylcholinesterase inhibitor E2020 in rhesus monkeys. Psychopharmacol(Berl), 131: 406-410.
Sahoo HB, Mandal PK, Bhattamisra SK, Bhaiji A, Sagar R. 2014. A new weapon for memory power: Elephantopus scaber (Linn.). Int J Nutr Pharmacol Neurol Dis, 4: 64-68.
Schmidt HD, Duman RS. 2007. The role of neurotrophic factors in adult hippocampal neurogenesis, antidepressant treatments and animal models of depressive-like behavior. Behav Pharmacol, 18: 391-418.
Shaji KS, Arun Kishore NR, Praveen Lal K, Prince M. 2002. Revealing a hidden problem: An evaluation of a community dementia case-finding program from the Indian 10/66 dementia research network. Int J Geriatr Psychiatry, 17: 222-225.
Shalam MD, Shantakumar SM, Narasu ML. 2007. Pharmacological and biochemical evidence for the antidepressant effect of the herbal preparation Trans-01. Indian J Pharmacol, 39: 231-234.
Shimohama S, Tanino H, Fujimoto S. 2001a. Differential expression of rat brain caspase family proteins during development and aging. Biochem Biophys Res Comm 289:1063-1066.
Shimohama S, Tanino H, Fujimoto S. 2001b. Differential subcellular localization of caspase family proteins in the adult rat brain. Neurosci Lett, 315:125-128.
Sivarajan VV, Balachandran I. 2002. Ayurvedic drugs and their plant sources. pp 271-272, Oxford and IBH publishing Co. Pvt. Ltd., New Delhi.
Smith RD, Kistler MK, Cohen-Williams M, Coffin VL. 1996. Cholinergic improvement of a naturally-occurring memory deficit in the young rat, Brain Res, 707: 13-21.
Stepanichev YM, Kudryashova IV, Yakovlev AA,  Onufriev MV, Khaspekov LG, Lyzhin AA, Lazareva NA, Gulyaeva NV. 2005. “Central administration of a caspase inhibitor impairs shuttle- box performance in rats,” Neuroscience, 136: 579-591.
Steru L, Chermat R, Thierry, B, Simon P. 1985. The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacol (Berl), 85: 367-370.
Tamilarasi T, Ananthi T. 2012. Phytochemical analysis and anti-microbial activity of Mimosa pudica Linn. Res J chem sci, 2: 72-74.Terry Jr AV, Buccafusco JJ. 2003. The cholinergic hypothesis of age and Alzheimer’s disease-related cognitive deficits: recent challenges and their implications for novel drug development. J Pharmcol Exp Ther, 306:821–7.
Thippeswamy BS, Mishra B, Veerapur VP, Gupta G. 2011. Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety. Indian J Pharmacol, 43: 50-55.
Tokumo K, Tamura N, Hirai T, Nishio H. 2006. Effects of (Z)-3-hexenol, a major component of green odor, on anxiety-related behavior of the mouse in an elevated plus-maze test and biogenic amines and their metabolites in the brain. Behav Brain Res, 166: 247-252.
Uutela P, Reinila R, Harju K, Piepponen P, Ketola RA. 2009. Analysis of Intact Glucuronides and Sulfates of Serotonin, Dopamine, and Their Phase I Metabolites in Rat Brain Microdialysates by Liquid Chromatography-Tandem Mass Spectrometry. Anal Chem, 81: 8417-8425.
Vaidyaratanm PS. 2001. Indian medicinal plants database, Vol 2. 1st ed. pp. 36-37.Orient Longman, Arya Vidyashala, Kottakkal.
Wei XY, Yang JY, Wang JH, Wu CF. 2007. Anxiolytic effect of saponins from Panax quinquefolium in mice. J Ethnopharmacol, 111:  613-618.
Willner P, Muscat R, Papp M. 1992. Chronic mild stress-induced anhedonia: a realistic model of depression. Neurosci BiobehavRev, 16:525-534.
Zheng M, Yajun F, Dongfang S, Chunming L. 2013. Antidepressant-like effect of flavonoids extracted from Apocynum venetum leaves on brain monoamine levels and dopaminergic system. J Ethnopharmacol, 147:108-113.
 
Avicenna Journal of Phytomedicine
Volume 6, Issue 6 - Serial Number 6
November and December 2016
Pages 696-710

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