Effects of Urtica dioica supplementation on blood lipids, hepatic enzymes and nitric oxide levels in type 2 diabetic patients: A double blind, randomized clinical trial

Document Type : Original Research Article

Authors

1 Young Researcher and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Physiology, Shahroud University of Medical Sciences, Shahroud, Iran

3 Department of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran

Abstract


Objective: Oxidative stress plays an important role in the development of diabetic complications including metabolic abnormality-induced diabetic micro-vascular and macro-vascular complications. Urtica dioica L. (U. dioica) has been traditionally used in Iranian medicine as an herbal remedy for hypoglycemic or due to its anti-inflammatory properties. The aim of the present study was to evaluate the effects of hydro-alcoholic extract of U. dioica on blood lipids, hepatic enzymes and nitric oxide levels in patients with type 2 diabetes mellitus.

Materials and Methods: 50 women with type 2 diabetes participated in this study and were randomly divided into two groups namely, control and intervention groups. Control group received placebo and intervention group received hydro-alcoholic extract of U. dioica. Before and after 8 weeks of continuous treatment, some biochemical serum levels including FPG, TG, SGPT, SGOT, HDL, LDL, SOD and NO were measured.

Results: The results indicated that after 8 weeks, in the intervention group, FPG, TG, and SGPT levels significantly decreased and HDL, NO and SOD levels significantly increased as compared to the control group.

Conclusion: Our results encourage the use of hydro-alcoholic extract of U. dioica as an antioxidant agent for additional therapy of diabetes as hydro-alcoholic extract of U. dioica may decrease risk factors of cardiovascular incidence and other complications in patients with diabetes mellitus.

Keywords

Main Subjects

References

Abo-elmatty D M, Essawy SS, Badr JM, Sterner O. 2013. Antioxidant andanti-inflammatoryeffectsof Urtica pilulifera extracts in type 2 diabetic rats. J Ethnopharmacol, 145: 269–277.
Ahangarpour A, Heidari H, Ramezani Ali Akbari F, Pakmehr M, Hajeye S, Ahmadi I, Mombeini Z, Babadi Hajani M. 2014. Effect of Boswellia serrata supplementation on blood lipid, hepatic enzymes and fructosamine levels in type2 diabetic patients. J Diabetes Metab Disord, 13: 1-5.
Bahmani M, Zargaran A, Rafieian Kopaei, Saki K. 2014. Bahmani .Ethnobotanical study of medicinal plants used in the management of diabetes mellitus in the Urmia, Northwest Iran. Asian Pac J Trop Med, 7: 348-354.
Celik I, and Tuluce Y. 2007. Elevation protective role of Camellia sinensis and Urtica dioica infusion against trichloroacetic acid-exposed in rats.  Phytother Res, 21: 1039–1044
Coskun O, Kanter M, Korkmaz A, Oter S, Quercetin a. 2005. Quercetin, a flavonoid antioxidant prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacol Res 51: 117-23.
Das M, Sarma BP, Rokeya B, Parial R, Nahar N, Mosihuzzaman M, Khan A, Ali L . 2012. Antihyperglycemic and antihyperlipidemic activity of Urtica dioica on type 2 diabetic model rats. J Diabetol, 2: 1-6.
Di Virgilio N. Papazogloub, EG, Jankauskienec Z, Di Lonardod S, Praczyke, M, Wielgusze K. 2014. The potential of stinging UD (Urtica dioica L.) as a crop with multiple uses. INDCRO. No of Pages 8.
Ebrahimzadeh MA, Gharekhani M, Ghorbani M, Dargany P. 2015. Effect of extract of aerial parts of Urtica dioica (Urticaceae) on the stability of soybean oil.  Trop J Pharm Res, 14: 125-131.
Farzami B, Ahmadvand D, Vardasbi S, Majin FJ, Khaghani S. 2003. Induction of insulin secretion by a component of Urtica dioica leave extract in perifused Islets of Langerhans and it’s in vivo effects in normal and streptozotocin diabetic rats. J Ethnopharmacol, 89: 47–53.
Golalipour MJ, Khori V. 2007. The protective activity of Urtica dioica leaves on blood glucose concentration and β-cells in streptozotocin-diabetic rats. Pak J Biol Sci, 10: 1200-1204.
Golalipour MJ, Kabiri Balajadeh B, Ghafari S, Azarhosh R, Khori V. 2011. Protective effect of Urtica dioica L. (Urticaceae) on morphometric and morphologic alterations of seminiferous tubules in STZ diabetic Rats. Iran J Basic Med Sci, 14: 472-477.
Ghosh R, Bhattacharya R, Bhattacharya G, Sinha AK. 2012. The control of stress induced type I diabetes mellitus in humans through the hepatic synthesis of insulin by the stimulation of nitric oxide production. Int J Biomed Sci, 8: 171-182.
Gupta SK. 2011. Intention to treat concept. Perspect Clin Res. 2: 109–112.
Harput US, Saracoglu I, Ogihara Y. 2005. Stimulation of Lymphocyte Proliferation and Inhibition of Nitric Oxide Production by Aqueous Urtica dioica Extract. Phytother Res, 19: 346–348.
Harvey M. 2007. Prism version 5.0 statistics guide. GraphPad Software, Inc. 255 pages.
Jeong SM, Kang MJ, Choi HN, Kim JH, Kim JI. 2012. Quercetin ameliorates hyperglycemia and dyslipidemia and improves antioxidant status in type 2 diabetic db/db mice. Nutr Res Pract, 6:201-207.
Joshi BC, Mukhija M,  Kalia AN. 2014. Pharmacognostical review of Urtica dioica L. Int J of G Pharmacy, 8: 201-209.
Kandis H, Karapolat S, Yildirim U, Saritas A, Gezer R. 2010. Memisogullari, Effects of Urtica dioica on hepatic ischemia-reperfusion injury in rats. Clinics (SaoPaulo), 65:1357–1361.
karim A,  Sohail MN, Munir S, Sattar S. 2011. Pharmacology and Phytochemistry of Pakistani Herbs and Herbal Drugs Used for Treatment of Diabetes. Int J Pharmacol, 7:419-439.
Khan A, Safdar M, Khan A, Anderson R. 2003. Cinnamon Improves Glucose and Lipids of people With Type 2 Diabetes. Diabetes Care, 26: 3115-8.
Khare V, Kushwaha P, Verma S, Gupta A, Srivastava S, Singh Rawat AK. 2012. Pharmacognostic evaluation and antioxidant activity of Urtica dioica L. Chin Med, 3: 128-135.
Mahluji S, Ostadrahimi AR, Mobasseri M,
Ebrahimzade Attari V, Payahoo L. 2013. Anti-inflammatory effects of Zingiber Officinale in type 2 diabetic patients. Adv. Pharm. Bull, 3: 273-276.
Mahmoud AH, Motawa HM, Wahba He, Ebrahim EY. 2006. Study of Some Antioxidant Parameters in Mice Livers Affected with Urtica pilulifera Extracts. Asian J of Bioch, 1: 67-74.
Malekirad AA, Hosseini N, Bayrami M, Hashemi T, Rahzani K, Abdollahi M. 2011. Benefit of Lemon Verbena in Healthy Subjects; Targeting Diseases Associated with Oxidative Stress. Asian J Anim Vet Adv, 6:953-957.
Malviya N, Jain S, Malviya S. 2010. Antidiabetic potential of medicinal plants. Acta Pol Pharm, 67:113–118.
Mamta S, Preeti k. 2014. Urtica dioica (Stinging nettle): A review of its chemical, pharmacological, toxicological and ethnomedical properties. Int J Pharm, 4: 270-277.
Manohar SM, Vaikasuvu SR, Deepthi K, Sachan A, Pemmaraju SR, Narasimha VL. 2013. An association of hyperglycemia with plasma malondialdehyde and atherogenic lipid risk factors in newly diagnosed Type 2 diabetic patients. J Res Med Sci, 18: 89–93.
Marazioti A, Bucci M, Coletta C, Vellecco V, Baskaran P, Szabo C, Cirino G, Marques AN, Guerreiro B, Goncalves AML, Seixas JD, Beuve A, Romao CC, Papapetropoulos A. 2011. Inhibition of nitric oxide–stimulated vasorelaxation by carbon monoxide-releasing molecules.  Arterioscler Thromb Vasc Biol, 31:2570-2575
Nassiri-Asl M, Zamansoltani F,  Abbasi E, Daneshi MM, Zangivand AA. 2009. Effectsof Urtica dioica extract on lipid profile in hypercholesterolemic rats. Zhong XiYi Jie He Xue Bao, 7: 428–433.
Nahata A, Dixit AK. 2012. Ameliorative effects of stinging U. dioica (Urtica dioica) ontestosterone-induced prostatic hyperplasia in rats. Andrologia, 44:396–409.
Nazemi N, Tarighat A, Bahrami A. 2012. The effect of hydro-alcoholic UD (Urtica dioica) extract on oxidative stress in patients with type 2 diabetes. A randomized double-blind clinical trial. Pak J Bio Sci, 15: 98-102.
Nojima H, Watanabe H, Yamane K, Kitahara Y, Sekikawa K, Yamamoto H, Yokoyama A. Inamizu T, Asahara T, Kohno N. 2008. Effect of aerobic exercise training on oxidative stress in patients with type 2 diabetes mellitus. Metabo Clini Exp, 57: 170–176.
Otles S.and Yalcin, B. 2012. Phenolic compounds analysis of root, stalk, and leaves of U. dioica. Sci World J, 1: 64-67.
Ozen T, Korkmaz H. 2003. Modulatory effect of Urtica dioica L. (Urticaceae) leaf extract on biotransformation enzyme systems, antioxidant enzymes, lactate dehydrogenase and lipid peroxidation in mice. Phytomedicine, 10:405-15.
Patience OO, Estella UO, Philip FU. 2014. The search for new hypoglycemic agents from plants. Afr. J. Pharm. Pharmacol, 8: 292-303.
Petal DK, Prasad SK, Hemalatha S. 2012. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed, 2:320-330
Patel SH, Udayabanu M. 2013. Effect of Urtica dioica on memory dysfunction and hypoalgesia in anexperimental model of diabetic neuropathy. Neuroscience Letters, 552: 114– 119.
37. Pourahmadi, M., Karimi Jashni, H., Bagheri, Mand. and Sotoodeh Jahromi, A. 2014. The effect of hydro-alcoholic extract of Urtica dioica Root on testes in adult rats. Life Sci. J, 11: 420-424.
Rains JL, Jain SK. 2011. Oxidative stress, insulin signaling and diabetes. Free Radical Biol Med, 50: 567-575.
Rasheed Z, Ahmad R, Rasheed N, Tripathi T, Ali R. 2008. Reactive oxygen species damaged hemoglobin present unique epitopes for type 1 diabetes autoantibodies. Int J Biol Chem, 2:1-13.
Tessari P, Cecchet D, Cosma A, Vettore A, Coracina A, Millioni R, Iori E. Puricelli L, Avogaro A. Vedovato M. 2010. Nitric oxide synthesis is reduced in subjects with type 2 diabetes and nephropathy. Diabetes 59: 2152-2159.
Testai L, Chericoni S. Calderone V, Nencioni G, Nieri P, Morelli I, Martinotti E. 2002.Cardiovascular effects of Urtica dioica L. (Urticaceae) roots extracts: in vitro and in vivo pharmacological studies. J. Ethnopharmacol, 81:105-109.
Tong PCY, Cockrum CS. 2003. Diabetes and its historical and social context: The epidemiology of type2 diabetes. Textbook of Diabetes. Blackwell Science Ltd. Massachusetts, USA, 3: 1-14.
Toldy A, Stadler K, Sasvari M, Jakus J, Jung KJ, Chung HY, Berkes I, Nyakas C, Radak Z. 2005. The effect of exercise and U. dioica supplementation on oxidative stress markers in the rat brain. Brain Res. Bull, 65: 487-493
Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E. 2003. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride induced hepatotoxicity in rats. IJPR, 17: 942–946.
 
Avicenna Journal of Phytomedicine
Volume 6, Issue 6 - Serial Number 6
November and December 2016
Pages 686-695

Files

Share

How to cite

Statistics