Evaluating bioactive constituents and toxicological effects of aqueous extract of fermented Pentaclethra macrophylla seeds in rats

Document Type : Original Research Article

Authors

1 Department of Biochemistry Abia State University, PMB 2000, Uturu, Abia State, Nigeria.

2 Department of Biochemistry, Faculty of Pure and Applied Science, Federal University Wukari, PMB 1020, Wukari, Taraba State, Nigeria.

3 Department of Medical Biochemistry, College of Medicine Enugu State University of Science and Technology, PMB 01660, Enugu-Nigeria.

Abstract

Objective: This study aimed at evaluating the bioactive constituents and the toxicological profile of the aqueous fermented seed extract of P. macrophylla.
Material and Methods: The chemical constituents of fermented P. macrophylla were assessed using GC-MS. For acute toxicity study, one-time doses of up to 5000 mg/kg of the extract were orally administered to male and female rats whereas 200, 400 and 800 mg/kg of the P. macrophylla extract were orally administered daily for 14 days in sub-acute toxicity investigation. Biochemical, haematological and lipid profiles were assessed following standard methods.
Results: Bioactive compounds such as citronellol and oxirane, tetradecyl- (hexadecylene oxide) were identified in the extract. In acute toxicity test, no death or sign of toxicity was identified. For sub-acute study, ALT decreased significantly (p<0.05) while HDL-C had dose-dependent increases. No effect (p<0.05) on haematological parameters except on platelets was found. No histopathological changes were observed.
Conclusion: Our results demonstrated that the extract of fermented P. macrophylla caused no toxic effects in the rats at the tested doses. Therefore, they may be termed safe for consumption and therapeutic uses.

Keywords

Main Subjects

References

Achinewhu SC. 1983. Protein quality of the African oil bean seed (Pentaclethra macrophylla). J Food Sci, 48: 1374-1375.
Adebayo JO, Adesokan AA, Olatunji LA, Buoro DO Soladoye AO. 2005. Effect of Ethanolic      extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in          rats. Biokemistri, 17: 45-50.
Afia ES, Momoh JA. 2006.  Standard operating procedure. Unpublished guideline for the chemical pathology laboratory, National Hospital, Abuja.
Agbor GA, Oben JE, Ngogang JY. 2005. Haematinic activity of Hibiscus cannabinus. Afr J         Biotechnol, 4(8): 833-837.
Akanji MA, Nafiu MO, Yakubu MT. 2008. Enzyme activities and histopathology of selected       tissues in rats treated with potassium bromate. Afr J Biomed Res, 11:87-95.
Akindahunsi AA. 2004. Physicochemical studies on African oil bean (Pentaclethra macrophylla Benth.) seed. J Food Agric Environ, 2: 14-17.
Ashafa AOT, Orekoya LO, Yakubu MT. 2012. Toxicity profile of ethanolic extract of Azadirachta indica stem bark in male Wistar rats. Asian Pac J Trop Biomed, 2: 811-   817.
Azubuine CE, Isu NR. 2006. Fungal contamination of fermenting African oil seeds ugba. Nig J    Microbiol, 20: 931-936.
Chidozie JI.  2006. African oil bean seed as an essential food supplement in the control of cancer and tobacco related diseases. Proceedings from the U1CC World Cancer Congress,          July 8-12, Washington DC.
Dioka C, Orisakwe OE, Afonne OJ, Agbasi PU, Akumka DD, Okonkwo CJ, Ilondu N. 2002. Investigation into the haematologic and hepatoxic effects of rinba-cin in rats. J Health Sci, 48: 393-398.
Ejiofor MAN, Oti E, Okafor JC. 1987. Studies on the fermentation of seeds of the African oil bean           tree (Pentaclethra macrophylla). Int Tree Crops J, 4: 135-144.
Enujiugha VN. 2003. Nutrient changes during the fermentation of African oil bean (Pentaclethra macrophylla Benth.) seeds. Pak J Nutr, 2003, 2: 320-323.
Enujiugha VN, Akanbi CT. 2005. Compositional changes in African oil bean (Pentaclethra         macrophylla Benth.) seeds during thermal processing. Pak J Nutr, 4: 27-31.
Eze VC, Onwuakor CE, Ukeka E. 2014. Proximate composition, biochemical and microbiological changes associated with fermenting African oil bean (Pentaclethra macrophylla Benth) Seeds. Am J Microbiol Res,2: 138-142.
Fisher DI, Safrany ST, Strike P, Mclennan AG, Cartwright JL. 2002. Nudix hydrolases that degrade dinucleoside and diphosphoinositol polyphosphates also have 5-phosphoribosyl            1-pyrophosphate (PRPP) pyrophosphatase activity that generates the glycolytic activator ribose 1, 5-bisphosphate. J Biol Chem, 277: 47313-47317.
Fungo R, Muyonga J, Kaaya A, Okia C, Tieguhong JC, Biadu-Forson JJ. 2015. Nutrients and      bioactive compounds content of Baillonella toxisperma, Trichoscypha abut and      Pentaclethra macrophylla from Cameroon. Food Sci Nutr, 3: 292-301.
Hajihosseini S, Setorki M. 2017. The antioxidant activity of Beta vulgaris leaf extract in improving scopolamine-induced spatial memory disorders in rats. Avicenna J Phytomed, 7: 417-425.
He S, Zhang H, Zeng X, Chen D, Yang P. 2013. Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis. Acta Pharmacol Sinica, 34: 1270-1283.
Isu N, Njoku H. 1997. An Evaluation of the microflora associated with fermented African oil       bean (Pentaclethra Macrophylla Bentham) seeds during Ugba production. Plant Foods    Hum Nutr, 51: 145-157.
Isu NR, Ofuya CO. 2000. Improvement of the traditional processing and fermentation of African oil bean (Pentaclethra macrophylla Benth) into a food snack-ugba. Int J Food Microbiol,      59: 235-239.
Jones AC, Robinson JM, Southwell KH. 1987. Investigation into Pentaclethra macrophylla seed oil: identification of hexacosanoic (C26:0) and octacosanoic (C28:0) fatty acids. J Sci        Food    Agric, 40: 189-194.
Kabuo NO, Uzuegbu JO, Ubbaonu CN, Oyeka EU. 2007. Effect of chemical compounds produced by microorganisms on organoleptic properties of ugba (Pentaclethra macrophylla Benth) during fermentation. Proceedings of the Annual Conference of          Nigerian Institute of Food Science and Technology, Oct. 22-25, Abuja, Nigeria, 175-176.
Manilal A, Sujith S, Sabarathnam B, Kiran G, Selvin J, Shakir C, Lipton A. 2011. Biological activity of the red alga Laurencia brandenii. Acta Bot Croat, 70: 81-90.
Mbajunwa OK, Akingbala JO, Mulongoy K, Oguntimein G. 1998. Starter culture evaluation for   the production of ugba from African oil bean seed (Pentaclethra macrophylla) [J]. J Sci Food Agric, 77: 127-132.
Monago CC, Ogbomeh PA, Joshua PE. 2004. Effect of African oil bean seed Pentaclethra macrophylla Benth on blood cholesterol level in rats. Global J Pure Applied Sci, 10: 165-168.
NIH. 1985. Care and Use of Laboratory Animals, National Institute of Health Publication, 85-123.
Njoku HO, Okemadu CP. 1989. Biochemical changes during the natural fermentation of the African oil bean (Pentaclethra macrophylla) for the production of Ugba. J Sci Food Agric, 49: 457-465.
Odeghe OB, Uwakwe AA, Monago CC. 2012. Some biochemical and haematological dtudies on the methanolic extract of Anthocleista grandiflora stem bark. Int J Appl Sci Technol, 2: 58-65.
Odoemelam SA. 2005. Proximate composition and selected physicochemical properties of the seeds of African oil bean (Pentaclethra marcrophylla). Pak J Nutr, 4: 382-383.
OECD. 2001. The OECD guidance document on acute oral toxicity testing; organization for        economic co-operation and development: Paris, France, pp. 1-14.
Ogueke CC, Aririatu LE. 2004. Microbial and organoleptic changes associated with ugba stored   at ambient temperature. Niger Food J, 22:133-140.
Ogueke CC, Nwosu JN, Owuamanam CI, Iwouno JN. 2010. Ugba, the fermented African oil       bean seeds; its production, chemical composition, preservation, safety and health benefits. Pak          J Biol Sci,13: 489-496.
Okwulehie IC. 2004. Insect pests and mycoflora of oil bean (Pentaclethra macrophylla Benth.)    pods and seeds in southeastern parts of Nigeria. Fruits, 59: 25-30.
Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Van Deun K, Smith P, Berger B, Heller A. 2000. Concordance of toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol, 32: 56-67.
Onwuliri VA, Attah I, Nwankwo JO. 2004. Anti-nutritional factors, essential and non-essential    fatty     acids composition of Ugba (Pentaclethra macrophylla) seeds at different stages of processing and fermentation. J Biol Sci, 4: 671-675.
Osagie-Eweka ESD, Alaiya TH. 2013. Effects of fermentation and heating on the functional properties of processed flour from African oil bean (Pentaclethra macrophylla benth) seeds. Afr J Food Agric Nutr Develop, 13: 8249-8257.
Ozer J, Ratner M, Shaw M, Bailey W, Schomaker S. 2008. The current state of serum biomarkers of hepatotoxicity. Toxicol, 245: 194-205.
Ravanbakhsh A, Mahdavi M, Jalilzade-Amin G, Javadi S, Maham M, Mohammadnejad D, Rashidi MR. 2016. Acute and subchronic toxicity study of the median septum of Juglans regia in Wistar rats. Adv Pharmaceut Bull, 6: 541-549.
Stone KD, Prussin C, Metcalfe DD. 2010. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol, 125:73-80.
Tarkang PA, Agbor GA, Tchamgoue DA, Yamthe TLR, David K, Ngadena YSM. 2012. Acute    and chronic toxicity studies of the aqueous and ethanol leaf extracts of Carica papaya       Linn. In wistar rats. J Nat Prod Plant Res, 2: 617-627.
Traesel GK, Menegati, SELT, dos Santos AC, Souza RIC, Boas GRV, Justi PN, Kassuya CAL, Argandoña EJS, Oesterreich SA. 2016. Oral acute and subchronic toxicity studies of the oil extracted from pequi (Caryocar brasiliense, Camb.) pulp in rats. Food Chem Toxicol, 97: 224-231.
Treinen-Moslen M. 2001.Toxic responses of the liver. In: Klaassen, C.D. (ed.). Casarett and Doull’s Toxicology: The Basic Sciences of Poisons. New York: McGraw-Hill, pp. 471-489.
 Udosen EO, Ifon ET. 1990. Fatty acid and amino acid composition of African oil beans (Pentaclethra macrophylla). Food Chem, 36: 155-160.
Ugbogu EA, Akubugwo IE, Ude VC, Gilbert J, Ekeanyanwu B. 2019. Toxicological evaluation of phytochemical characterized aqueous extract of wild dried Lentinus squarrosulus      (Mont.) mushroom in rats. Toxicol Res, 35: 181-190.
Ugbogu AE, Arunsi UO, Uche-Ikonne OO, Ude VC, Okezie E. 2017. Antiulcerogenic potentials of fermented aqueous extract of Pentaclethra macrophylla (Benth) seeds. Am J  Biomed     Res, 5: 57-64.
Ugbogu AE, Okezie E, Uche-Ikonne  C, Duru M, Atasie OC. 2016. Toxicity evaluation of the aqueous stem extracts of Senna alata in Wistar Rats. Am J Biomed Res, 4: 80-86.
Ugbogu EA, Ude VC, Elekwa I, Arunsi UO, Uche-Ikonne C, Nwakanma C. 2018. Toxicological profile of the aqueous-fermented extract of Musa paradisiaca in rats. Avicenna J      Phytomed, 8: 478-487.
Wasan KM, Najafi S, Wong J, Kwong M. 2001. Assessing plasma lipid levels, body weight, and hepatic and renal toxicity following chronic oral administration of a water soluble phytostanol compound, FM-VP4, to gerbils. J Pharm Pharm Sci, 4: 228-34.
WHO. 2003. WHO guidelines on good agricultural and collection practices (GACP) for   medicinal plants. World Health Organization, Geneva.
Witthawasku P, Ampai P, Kanjanapothi D, Taesothikula T, Lertprasertsuke N.  2003. Acute and sub-acute toxicities of saponin mixture isolated from Schefflera leucantha Viguier. J            Ethnopharmacol,89: 115-121.
Zhuang SR, Chen SL, Tsai JH, Huang CC, Wu TC, Liu WS, Tseng HC, Lee HS, Huang MC, Shane         GT, Yang CH, Shen YC, Yan YY, Wang CK. 2009. Effect of citronellol and the Chinese medical herb complex on cellular immunity of cancer patients receiving chemotherapy/radiotherapy. Phytother Res, 23: 785-790.
Avicenna Journal of Phytomedicine
Volume 10, Issue 1
January and February 2020
Pages 101-113

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