Zataria multiflora Boiss. extract improves spatial memory and learning capacity in scopolamine-induced amnesic rats

Document Type : Original Research Article

Authors

1 Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

2 Pharmaceutical Research Center, Neuroscience Institute, Department of Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.

3 Herbal and Traditional Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

Objective: Zataria multiflora (Zm) has been proposed for memory enhancing in Persian traditional medicine; but to now, no study has been carried out in this field yet. The aim of this research was to study the plant effect on spatial memory in scopolamine-induced amnesia and investigate in vivo anticholinesterase effect of Zm.
Material and Methods: Aerial parts of the plant were extracted with methanol and standardized on the basis of rutin content. Male rats received three doses of Zm extract (100, 200 and 400 mg/kg, intraperitoneal (ip) for 7 days) and 30 min after the latest dose, scopolamine (1 mg/kg) was administered to animals. Learning capacity and spatial memory were studied using morris water maze (MWM) and passive avoidance test (PAT) methods. Anticholinesterase activity was studied using Ellman’s method. Physostigmine (0.3 mg/kg) and piracetam (200 mg/kg) were used as positive controls.
Results: All doses of Zm extract significantly decreased the distance and time spent to find the platform in MWM and increased the time latency in PAT test. In both MWM and PAT tests, the highest effect of Zm was observed at 200 mg/kg which was in accordance with AChE inhibitory effect of the plant.
Conclusion: Our findings indicate that Zm has anti-amnesic effect and might improve memory deficit through anticholinesterase activity.

Keywords

Main Subjects

References

Ahmadipour A, Sharififar F, Najafi A, Atashbar J, Karami-Mohajeri S. 2015. Preventive effect of methanolic extract of Zataria Multiflora Boiss on liver toxicity of paracetamol in rats. J Med Life, 8:270–274.
Airoldi C, Sironi E, Dias C, Marcelo F, Martins A, Rauter AP, Nicotra F, Jimenez-Barbero J. 2013. Natural compounds against Alzheimer’s disease: molecular recognition of Abeta1-42 peptide by Salvia sclareoides extract and its major component, rosmarinic acid, as investigated by NMR. Chem Asian J, 8:596–602.
Alkam T, Nitta A, Mizoguchi H, Itoh A, Nabeshima T. 2007. A natural scavenger of peroxynitrites, rosmarinic acid, protects against impairment of memory induced by Abeta(25-35). Behav Brain Res, 180:139–145.
Ansari M, Etelaei F, Sharififar F. 2011. Tyrosinase kinetic in the presence of three inhibitory medicinal plants. Int J Green Pharm, 5: 103-106.
Arabzadeh AM, Ansari-Dogaheh M, Sharififar F, Shakibaie M, Heidarbeigi M. 2013. Anti herpes simplex-1 activity of a standard extract of Zataria multiflora Boiss. Pak J Biol Sci, 16; 180-184.
Barati M, Sharifi I, Sharififar F. 2010. In vitro evaluation of anti-Leishmanial activities of Zataria multiflora Boiss, Peganum harmala and Myrtus communis by colorimetric assay. J Kerman Univ Med Sci, 17; 32-41.
Chaiyana W, Rades T, Okonogi S. 2013. Characterization and in vitro permeation study of microemulsions and liquid crystalline systems containing the anticholinesterase alkaloidal extract from Tabernaemontana divaricata. Int J Pharm, 452:201–210.
Dehghan-Noodeh G, Sharififar F, Moshafi MH, Behravan E, Dehghan-Noodeh A, Rezaei-Gharaeei R. 2013. Antimutagenicity activity of different fractions of Zataria multiflora, Achillea wilhelmsii and Camellia sinensis using Ames test. J Med Sci, 13; 459-464.
Freddi R, Duca P, Gritti I, Mariotti M, Vertemati M. 2009. Behavioral and degeneration changes in the basal forebrain systems of aged rats:  a quantitative study in the region of the basal forebrain after levo-acetyl-carnitine treatments assessed by Abercrombie estimation. Prog Neuropsychopharmacol Biol Psychiatry, 33:419–426.
Garcia-Pardo MP, Roger-Sanchez C, Rodriguez-Arias M, Minarro J, Aguilar MA. 2017. Cognitive and behavioural effects induced by social stress plus MDMA administration in mice. Behav Brain Res, 319:63–72.
Giulian D, Haverkamp LJ, Yu JH, Karshin W, Tom D, Li J, Kirkpatrick J, Kuo LM, Roher AE. 1996. Specific domains of beta-amyloid from Alzheimer plaque elicit neuron killing in human microglia. J Neurosci, 16:6021–6037.
Gulcin I, Scozzafava A, Supuran CT, Koksal Z, Turkan F, Cetinkaya S, Bingol Z, Huyut Z, Alwasel SH. 2016. Rosmarinic acid inhibits some metabolic enzymes including glutathione S-transferase, lactoperoxidase, acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase isoenzymes. J Enzyme Inhib Med Chem, 31:1698–1702.
Guo C, Shen J, Meng Z, Yang X, Li F. 2016. Neuroprotective effects of polygalacic acid on scopolamine-induced memory deficits in mice. Phytomedicine, 23:149–155.
Harandi S, Golchin L, Ansari M, Moradi A, Shabani M, Sheibani V. 2015. Antiamnesic Effects of Walnuts Consumption on Scopolamine-Induced Memory Impairments in Rats. Basic Clin Neurosci, 6:91–99.
Kalaycioglu Z, Gazioglu I, Erim FB. 2017. Comparison of antioxidant, anticholinesterase, and antidiabetic activities of three curcuminoids isolated from Curcuma longa L. Nat Prod Res, 31:2914–2917.
Khanam H, Ali A, Asif M, Shamsuzzaman. 2016. Neurodegenerative diseases linked to misfolded proteins and their therapeutic approaches: A review. Eur J Med Chem, 124:1121–1141.
Li R, Zang A, Zhang L, Zhang H, Zhao L, Qi Z, Wang H. 2014. Chrysin ameliorates diabetes-associated cognitive deficits in Wistar rats. Neurol Sci Off J Ital Neurol Soc Ital Soc Clin Neurophysiol, 35:1527–1532.
Mandegary A, Pournamdari M, Sharififar F, Pournourmohammadi S, Fardiar R, Shooli S. 2012. Alkaloid and flavonoid rich fractions of fenugreek seeds  with antinociceptive and anti-inflammatory effects. Food Chem Toxicol, 50: 2503-2507
Mandegary A, Sharififar F, Abdar M. 2013. Anticonvulsant effect of the essential oil and methanolic extracts of Zataria multiflora boiss. Cent Nerv Syst Agents Med Chem, 13: 93-97.
Mandegary A, Sharififar F, Soodi M, Zarei M. 2014a. Anticholinesterase effect of soybean extract (Glycine max l.) in the brain of amnesic mice and its protective effects against beta-amyloid induced cytotoxicity in pc12 cells. 2: 61–65.
Mandegary A, Soodi M, Sharififar F, Ahmadi S. 2014b. Anticholinesterase, antioxidant, and neuroprotective effects of Tripleurospermum disciforme and Dracocephalum multicaule. J Ayurveda Integr Med, 5: 162-166.
Mayford M, Siegelbaum SA, Kandel ER. 2012. Synapses and memory storage. Cold Spring Harb Perspect Biol, 4; a005751.
Mohamadi N, Sharififar F, Koohpayeh A, Daneshpajouh M. 2015. Traditional and Ethnobotanical uses of medicinal plants by ancient populations in Khabr and Rouchon of Iran. J Appl Pharm Sci, 5: 101-107.
Momen H. 2009. Tohfe Almomenin. Tehran: The Parliament Library.116
Qin Q-Z, Zhao Q, Chen C-H, Zhou Z, Yu Z-P. 2015. [Rutin protects against trimethyltin-induced learning and memory impairment in BALB/c mice by antagonizing down-regulated synaptophysin expression]. Nan Fang Yi Ke Da Xue Xue Bao, 35:113–116.
Sayeed MS Bin, Asaduzzaman M, Morshed H, Hossain MM, Kadir MF, Rahman MR. 2013. The effect of Nigella sativa Linn. seed on memory, attention and cognition in healthy human volunteers. J Ethnopharmacol, 148:780–786.
Sayeed MS Bin, Shams T, Fahim Hossain S, Rahman MR, Mostofa A, Fahim Kadir M, Mahmood S, Asaduzzaman M. 2014. Nigella sativa L. seeds modulate mood, anxiety and cognition in healthy adolescent males. J Ethnopharmacol, 152:156–162.
Sharififar F, Koohpayeh A, Motaghi MM,Amirkhosravi A,Pourmohseni nasab E, Khodashenas M. 2011. Ethnobotanic study of medicinal plants in Sirjan, Kerman Province, Iran. JHD, 1:19–28.
Sharififar F, Moharamkhani MR, Moattar F, Babakhanloo P, Khodami M. 2014. Ethnobotanical study of medicinal plants of Joopar mountains of Kerman province, Iran. J Kerman Univ Med Sci, 21:37–51.
Sharififar F, Dehghan-Noudeh G, Moshafi M, Ohadi M, Zaman-Basir M, Yazdanpanah E, Yusefian S. 2015. Antimutagenic activity of major fractions of Zataria multiflora Boiss by Ames method. Asian J Pharm, 9: 459-464.
Sharififar F, Derakhshanfar A, Dehghan-Nudeh G, Abbasi N, Gharaei RR, Koohpayeh A, Daneshpajouh M. 2011a. In vivo antioxidant activity of zataria multiflora boiss essential oil. Pak J Pharm Sci, 24: 221-225.
Sharififar F, Mirtajadini M, Azampour MJ, Zamani E. 2011b. Essential oil and methanolic extract of Zataria multiflora boiss with anticholinesterase effect. Pakistan J Biol Sci, 15: 1-5.
Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M. 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control, 18: 800-805.
Stuchlik A. 2014. Dynamic learning and memory, synaptic plasticity and neurogenesis: an update. Front Behav Neurosci, 8:1-6.
Avicenna Journal of Phytomedicine
Volume 9, Issue 6
November and December 2019
Pages 587-596

Files

Share

How to cite

Statistics