Cytotoxic and anti-proliferative effects of Rosa beggeriana Schrenk extracts on human liver and breast cancer cells

Document Type : Original Research Article

Authors

1 Department of Biotechnology, Graduate University of Advanced Technology, Kerman, Iran.

2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology Kerman, Iran.

Abstract

Objective: Rosa beggeriana Schrenk has been consumed in Iranian traditional medicine. In contrary to its close species (e.g. R. canina), there is no data on its medicinal properties. Therefore, we explored possible cytotoxic effects of R. beggeriana against two cancer cell lines.
Materials and Methods: The cytotoxic and anti-proliferative effects of R. beggeriana ethanolic and aqueous extracts on human liver cancer cells (LCLPI 11), breast cancer cells (MCF-7) and fibroblast-like cells (HSkMC) were evaluated by MTT, BrdU and TUNEL assays. 
Results: Following 48 h, IC50 values for LCL-PI11 and MCF-7 cells were found to be 3.9 and 4.2 μg/mL for aqueous extract, and 2.3 and 2.7 μg/mL for ethanolic extract, respectively.
BrdU assay data verified the MTT results and showed that both extracts inhibit cell proliferation as much as 5-fluorouracil does (p<0.05). The ethanolic extract had a more marked inhibitory effect compared to the aqueous extract (p<0.05). Besides both extracts were less effective against HSKMC cells compared to other cells lines.
TUNEL assay results demonstrated that following 48 h, the aqueous extract induced about 19 and 24% apoptotic death in the LCL-PI 11 and MCF-7 cells, respectively (p<0.05). While at the same time, the ethanolic extract was more potent and caused about 83 and 91% death in the LCL-PI 11 and MCF-7 cells, respectively (p<0.05).
Conclusion: These data indicate that both extracts have anti-proliferative and pro-apoptotic activities on these two cancer cell lines and these effects were more pronounced then their activities against normal cells. Also, the ethanolic extract was more potent than the aqueous extract. Further researches are necessary for finding and isolating effective anticancer ingredient of R. beggeriana.

Keywords

Main Subjects

References

Amiri MS, Joharchi MR. 2013. Ethnobotanical investigation of traditional medicinal plants commercialized in the markets of Mashhad, Iran. Avicenna J Phytomed, 3: 254-271.
Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, Temml V, Wang L, Schwaiger S, Heiss EH, Rollinger JM, Schuster D, Breuss JM, Bochkov V, Mihovilovic MD, Kopp B, Bauer R, Dirsch VM, Stuppner H. 2015. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv, 33: 1582-1614.
Chrubasik C, Roufogalis BD, Muller-Ladner U, Chrubasik S. 2008. A systematic review on the Rosa canina effect and efficacy profiles. Phytother Res, 22: 725-733.
Dai W, Gao Q, Qiu J, Yuan J, Wu G, Shen G. 2015. Quercetin induces apoptosis and enhances 5-FU therapeutic efficacy in hepatocellular carcinoma. Tumor Biol, 5: 6307–6313.
Deliorman Orhan D, Hartevioglu A, Kupeli E, Yesilada E. 2007. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits. J Ethnopharmacol, 112: 394-400.
Duo J, Ying GG, Wang GW, Zhang L. 2012. Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation. Mol Med Rep, 5: 1453–1456.
Falahi E, Farhadi J, Abdollahpoor F, Ghasemi M, Kakai Z, Karimi Z. 2014. Antioxidant and cytotoxic effects of aqueous and ethanolic extracts of R.canina against U937 cell line. Yafteh, 17: 15-24.
Fujii T, Saito M, 2009. Inhibitory effect of quercetin isolated from rose hip (Rosa canina L.) against melanogenesis by mouse melanoma cells. Biosci Biotechnol Biochem, 73: 1989-1993.
GLOBOCAN 2018 at: https://gco.iarc.fr/today/data/factsheets/cancers/39-All-cancers-fact-sheet.pdf
Jimenez S, Gascon S, Luquin A, Laguna M, Ancin-Azpilicueta C, Rodriguez-Yoldi MJ. 2016. Rosa canina extracts have antiproliferative and antioxidant effects on caco-2 human colon cancer. PLoS One, 11: e0159136.
Jimenez S, Jimenez-Moreno N, Luquin A, Laguna M, Rodriguez-Yoldi MJ, Ancin-Azpilicueta C. 2017. Chemical composition of rosehips from different Rosa species: an alternative source of antioxidants for the food industry. Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 34:1121-1130.
Lattanzio F, Greco E, Carretta D, Cervellati R, Govoni P, Speroni E. 2011. In vivo anti-inflammatory effect of Rosa canina L. extract. J Ethnopharmacol, 137: 880-885.
McCloud TG, 2010. High throughput extraction of plant, marine and fungal specimens for preservation of biologically active molecules. Molecules, 15: 4526-4563.
Nadpal JD, Lesjak MM, Sibul FS, Anackov GT, Cetojevic-Simin DD, Mimica-Dukic NM, Beara IN. 2016. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds. Food Chem, 192: 907-914.
Newman DJ, Cragg GM. 2016. Natural Products as Sources of New Drugs from 1981 to 2014. J Nat Prod, 79: 629-661.
Rajkapoor B, Sankari M, Sumithra M, Anbu J, Harikrishnan N, Gobinath M, Suba V, Balaji R. 2007. Antitumor and cytotoxic effects of Phyllanthus polyphyllus on Ehrlich ascites carcinoma and human cancer cell lines. Biosci Biotechnol Biochem, 71: 2177-2183.
Roman I, Stanila A, Stanila S. 2013. Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chem Cent J, 7:73
Tariq A, Sadia S, Pan K, Ullah I, Mussarat S, Sun F, Abiodun OO, Batbaatar A, Li Z, Song D, Xiong Q, Ullah R, Khan S, Basnet BB, Kumar B, Islam R, Adnan M. 2017. A systematic review on ethnomedicines of anti-cancer plants. Phytotherapy Research, 31: 202-264.
Tumbas, V. T., J. M. Canadanovic-Brunet, D. D. Cetojevic-Simin, G. S. Cetkovic, S. M. Ethilas, Gille L. 2012. Effect of rosehip (Rosa canina L.) phytochemicals on stable free radicals and human cancer cells. J Sci Food Agric, 92: 1273-1281.
WHO (https://www.who.int/news-room/fact sheets/detail/the-top-10-causes-of-death).
Zhou Y, Zheng J, Li Y,  Xu DP, Li S, Chen YM, Li HB. 2016. Natural Polyphenols for Prevention and Treatment of Cancer. Nutrients, 8: 515.
 
Avicenna Journal of Phytomedicine
Volume 9, Issue 4
July and August 2019
Pages 386-395

Files

Share

How to cite

Statistics