Effect of carvacrol on pulmonary function tests, and total and differential white blood cell counts in healthy volunteers: A randomized clinical trial

Document Type : Original Research Article

Authors

1 Pharmaciutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Dental Materials Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective: This is the first study to evaluate the effect of carvacrol on pulmonary function tests (PFT), and total and differential white blood cell (WBC) counts in healthy volunteers.
Materials and Methods: Thirty healthy volunteers were recruited based on the inclusion and exclusion criteria. The subjects were treated with two doses of carvacrol (1 and 2 mg/kg/day) for 1 month. Total and differential WBC counts and PFT were examined before and after the treatment period.
Results: There were no statistically significant differences in terms of total and differential WBC counts between pre and post-treatment with the two doses of carvacrol. Also, results of PFT tests indicated that administration of 1 mg/kg/day carvacrol had no effect on PFT parameters when comparing post-treatment values with pre-treatment values. Treatment with 2 mg/kg/day carvacrol for 1 month increased forced expiratory volume in first second (FEV1) (p<0.05). However, the changes in total and differential WBC counts as well as PFT values after 1-month treatment were not significantly different between two groups.
Conclusion: The results of this study indicated that treatment of healthy individuals with 1 and 2 mg/kg carvacrol for 1 month has no negative effects on total and differential WBC counts nor PFT values.

Keywords

Main Subjects

References

Ahmad A, Khan A, Akhtar F, Yousuf S, Xess I, Khan L, Manzoor N. 2011. Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. Eur J Clin Microbiol Infect Dis, 30:41-50.
Akrom A, Darmawan E. 2017. Tolerability and safety of black cumin seed oil (Bcso) administration for 20 days in healthy subjects. Biomed Res, 28: 4196-4201
Andersen A. 2005. Final report on the safety assessment of sodium p-chloro-m-cresol, p-chloro-m-cresol, chlorothymol, mixed cresols, m-cresol, o-cresol, p-cresol, isopropyl cresols, thymol, o-cymen-5-ol, and carvacrol. J Toxicol, 25:29-127.
Arunasree K. 2010. Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine, 17:581-588.
Boskabady M, Jafari Z, Pouraboli I. 2011. The effect of carvacrol on muscarinic receptors of guinea‐pig tracheal chains. Phytother Res, 25:530-535.
Boskabady M, Jandaghi P. 2003. Relaxant effects of carvacrol on guinea pig tracheal chains and its possible mechanisms. Die Pharmazie- Int J Pharm Sci, 58:661-663.
Boskabady MH, Gholami Mahtaj L. Lung inflammation changes and oxidative stress induced by cigarette smoke exposure in guinea pigs affected by Zataria multiflora and its constituent, carvacrol. BMC Complement Altern Med. 2015; 15: 39.
Boskabady MH, Jalali S, Yahyazadeh N, Boskabady M. 2016. Carvacrol attenuates serum levels of total protein, phospholipase A2 and histamine in asthmatic guinea pig. Avicenna J Phytomed, 6:636-642.
Boskabady MH, Jalali S. 2013. Effect of carvacrol on tracheal responsiveness, inflammatory mediators, total and differential WBC count in blood of sensitized guinea pigs. Exp Biol Med, 238:200-208.
Boskabady MH, Kaveh M, Eftekhar N, Nemati A. 2010. Zataria multiflora boiss and carvacrol affect β2-adrenoceptors of guinea pig trachea. J Evid Based Complementary Altern Med, 2011:1-9.
Boskabady MH, Tabanfar H, Gholamnezhad Z, Sadeghnia HR. 2012. Inhibitory effect of Zataria multiflora Boiss and carvacrol on histamine (H1) receptors of guinea‐pig tracheal chains. Fundam Clin Pharmacol, 26:609-620.
Boskabady MH, Tabatabaee A, Jalali S. 2014. Potential effect of the extract of Zataria multiflora and its constituent, carvacrol, on lung pathology, total and differential WBC, IgE and eosinophil peroxidase levels in sensitized guinea pigs. J Funct Foods, 11:49-61.
Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol, 94:223-253.
Busse WW, Swenson CA. 1989. The relationship between plasma histamine concentrations and bronchial obstruction to antigen challenge in allergic rhinitis. J allergy clin immunolo, 84:658-666.
Can Baser K. 2008. Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Curr Pharm Des, 14:3106-3119.
De Moraes MEA, Bezerra MM, Bezerra FAF, De Moraes RA, Cavalcanti PP, Uchoa CRA, Lima FAV, De Moraes MO. 2008. Safety evaluation of Elixir paregorico® in healthy volunteers: a phase I study. Hum Exp Toxicol, 27: 751-756.
De Vincenzi M, Stammati A, De Vincenzi A, Silano M. 2004. Constituents of aromatic plants: carvacrol. Fitoterapia, 75:801-804.
Ebrahimzadeh H, Yamini Y, Sefidkon F, Chaloosi M, Pourmortazavi SM. 2003. Chemical composition of the essential oil and supercritical CO 2 extracts of Zataria multiflora Boiss. Food Chem, 83:357-361.
Fachini-Queiroz FC, Kummer R, Estevao-Silva CF, Carvalho MDdB, Cunha JM, Grespan R, Bersani-Amado CA, Cuman RKN. 2012. Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response. Evid Based Complementary Altern Med, 2012:1-10. 
Farraj AK, Harkema JR, Jan T-R, Kaminski NE. 2003. Immune responses in the lung and local lymph node of A/J mice to intranasal sensitization and challenge with adjuvant-free ovalbumin. Toxicol Pathol, 31:432-447.
Gholami Mahtaj L, Boskabady M, Mohamadian Roshan N. 2015. The effect of Zataria multiflora and its constituent, carvacrol, on tracheal responsiveness and lung pathology in guinea pig model of COPD. Phytother Res, 29:730-736.
Gholami Mahtaj L, Boskabady M, Mohamadian Roshan N. 2015. The effect of Zataria multiflora and its constituent, carvacrol, on tracheal responsiveness and lung pathology in guinea pig model of COPD. Phytother Res, 29: 730-736.
Gholamnezhad Z, Havakhah S, Boskabady MH. 2016. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J Ethnopharmacol, 190:372-786.
Hagan E, Hansen W, Fitzhugh O, Jenner P, Jones W, Taylor JM, Long EL, Nelson A, Brouwer J. 1967. Food flavourings and compounds of related structure. II. Subacute and chronic toxicity. Food Cosmet Toxicol, 5:141-157.
Jalali S, Boskabady MH, Rohani AH, Eidi A. 2013. The effect of carvacrol on serum cytokines and endothelin levels of ovalbumin sensitized guinea-pigs. Iran J Basic Med Sci, 16:615-619.
Jayakumar S, Madankumar A, Asokkumar S, Raghunandhakumar S, Kamaraj S, Divya MGJ, Devaki T. 2012. Potential preventive effect of carvacrol against diethylnitrosamine-induced hepatocellular carcinoma in rats. Mol Cell Biochem, 360:51-60.
Kianmehr M, Rezaei A, Boskabady MH. 2016. Effect of carvacrol on various cytokines genes expression in splenocytes of asthmatic mice. Iran J Basic Med Sci, 19:402-410.
Kohlert C, Schindler G, Marz RW, Abel G, Brinkhaus B, Derendorf H, Grafe EU, Veit M. 2002.  Systemic availability and pharmacokinetics of thymol in humans. J Clin Pharmacol, 42: 731-737.
Landa P, Kokoska L, Pribylova M, Vanek T, Marsik P. 2009.  In vitro anti-inflammatory activity of carvacrol: Inhibitory effect on COX-2 catalyzed prostaglandin E 2 biosynthesisb. Arch Pharm Res, 32:75-78.
Liolios CC, Gortzi O, Lalas S, Tsaknis J, Chinou I. 2009. Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial ctivity. Food Chem, 112:77–83.
Livingston AE. 1970. The comparative toxicity of thymol and carvacrol (isothymol). Public Health Rep, 1921:1317-1331.
Mahtaj LG, Feizpour A, Kianmehr M, Soukhtanloo M, Boskabady MH. 2015. The effect of carvacrol on systemic inflammation in guinea pigs model of COPD induced by cigarette smoke exposure. Pharmacol Rep, 67:140-145.
McOmie W, Anderson HH, Estess F. 1949. Comparative toxicity of certain t-butyl substituted cresols and xylenols. Journal of the American Pharmaceutical Association (Scientific ed), 38:366-369.
Mesia K, Cimanga K, Tona L, Mampunza MM, Ntamabyaliro N, Muanda T, Muyembe T, Totté J, Mets T, Pieters L, Vlietinck A. 2011. Assessment of the short-term safety and tolerability of a quantified 80% ethanol extract from the stem bark of Nauclea pobeguinii (PR 259 CT1) in healthy volunteers: a clinical phase I study. Planta Med, 77: 111–116.
Michiels J, Missotten J, Dierick N, Fremaut D, Maene P, De Smet S. 2008. In vitro degradation and in vivo passage kinetics of carvacrol, thymol, eugenol and transcinnnamaldehyde along the gastrointestinal tract of piglets. J Sci Food Agric, 88: 2371-2381.
Özkan A, Erdoğan A. 2011. A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turk J Biol, 35:735-742.
Ravishankar S, Zhu L, Law B, Joens L, Friedman M. 2008. Plant-derived compounds inactivate antibiotic-resistant Campylobacter jejuni strains. J Food Prot, 71:1145-1149.
Reagan-Shaw S, Nihal M, Ahmad N. 2008. Dose translation from animal to human studies revisited. FASEB J, 22:659-661.
Sanjay B, Dinesh S, Neha S. 2012. Stability Testing of Pharmaceutical Products. J Appl Pharm Sci, 03:129-138
Shin JW, Seol IC, Son CG. 2010. Interpretation of animal dose and human equivalent dose for drug development. J Korean Med, 31:1-7.
Stammati A, Bonsi P, Zucco F, Moezelaar R, Alakomi H-L, Von Wright A. 1999. Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Food Chem Toxicol, 37:813-823.
Suntres ZE, Coccimiglio J, Alipour M. 2015. The bioactivity and toxicological actions of carvacrol. Crit Rev Food Sci Nutr, 55:304-318.
Avicenna Journal of Phytomedicine
Volume 9, Issue 2
March and April 2019
Pages 134-142

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