<?xml version="1.0" encoding="utf-8"?>
			<journal>
			<title>Avicenna Journal of Phytomedicine</title>
			<title_fa></title_fa>
			<short_title></short_title>
			<subject>Medical Sciences</subject>
			<web_url>https://ajp.mums.ac.ir/</web_url>
			<journal_hbi_system_id>0</journal_hbi_system_id>
			<journal_hbi_system_user></journal_hbi_system_user>
			<journal_id_issn>2228-7930</journal_id_issn>
			<journal_id_issn_online>2228-7949</journal_id_issn_online>
			<journal_id_pii></journal_id_pii>
			<journal_id_doi></journal_id_doi>
			<journal_id_iranmedex></journal_id_iranmedex>
			<journal_id_magiran></journal_id_magiran>
			<journal_id_sid></journal_id_sid>
			<journal_id_nlai></journal_id_nlai>
			<journal_id_science></journal_id_science>
			<language>en</language>
			<pubdate>
				<type>jalali</type>
				<year>0</year>
				<month>0</month>
				<day>1</day>
			</pubdate>
			<pubdate>
				<type>gregorian</type>
				<year>2015</year>
				<month>3</month>
				<day>1</day>
			</pubdate>
			<volume>5</volume>
			<number>2</number>
			<publish_type>online</publish_type>
			<publish_edition>1</publish_edition>
			<article_type>fulltext</article_type>
			<articleset><article>
				<language>en</language>
				<article_id_issn></article_id_issn>
				<article_id_issn_online></article_id_issn_online>
				<article_id_pubmed></article_id_pubmed>
				<article_id_pii></article_id_pii>
				<article_id_doi></article_id_doi>
				<article_id_iranmedex></article_id_iranmedex>
				<article_id_magiran></article_id_magiran>
				<article_id_sid></article_id_sid>
				<title_fa></title_fa>
				<title>Cytoprotective and antioxidant effects of Echium amoenum anthocyanin-rich extract in human endothelial cells (HUVECs)</title>
				<subject_fa></subject_fa>
				<subject></subject>
				<content_type_fa></content_type_fa>
				<content_type>Original Research Article</content_type>
				<abstract_fa><![CDATA[]]></abstract_fa>
				<abstract><![CDATA[Objective: Echium amoenum Fisch. &amp; C.A. Mey. is used for the treatment of various diseases in traditional medicine. This plant is a major source of anthocyanins with beneficial cardiovascular properties such as anti-atherosclerotic and antihypertensive effects. In the present study, the protective and antioxidant effects of anthocyanin-rich E. amoenum extract were evaluated on human vascular endothelial cells (HUVECs) under oxidative stress. Materials and Methods: Cell viability and oxidative status were assessed on H2O2-induced oxidative stress (0.5 mM H2O2 for 2 h) in HUVECs pretreated by anthocyanin-rich extract from the petals of E. amoenum (25-1000 µg/ml). Cytoprotective effect of the extract was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were assessed in intra- and extra-cellular fluid of pretreated cells. Results: Pretreatment of HUVECs with E. amoenum extract at the concentrations of 100-1000 µg/ml reduced the cell death resulted from the exposure to H2O2 in a concentration-dependent manner. E. amoenum extract decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluid at different concentration ranges. Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml. Conclusion: These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of E. amoenum against H2O2-induced oxidative stress in HUVECs. However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.]]></abstract>
				<keyword_fa></keyword_fa>
				<keyword>Echium amoenum, HUVECs, Oxidative stress, Antioxidant</keyword>
				<start_page>157</start_page>
				<end_page>166</end_page>
				<web_url>https://ajp.mums.ac.ir/article_3947.html</web_url>
			<author_list><author>
				<first_name>Leila</first_name>
				<middle_name></middle_name>
				<last_name>Safaeian</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>leila_safaeian@pharm.mui.ac.ir</email>
				<code>15165</code>
				<coreauthor>Yes</coreauthor>
				<affiliation>Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Shaghayegh</first_name>
				<middle_name></middle_name>
				<last_name>Haghjoo Javanmard</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>shaghayeghhaghjoo@yahoo.com</email>
				<code>15166</code>
				<coreauthor>No</coreauthor>
				<affiliation>Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Mustafa</first_name>
				<middle_name></middle_name>
				<last_name>Ghanadian</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>ghanadian@pharm.mui.ac.ir</email>
				<code>15167</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Sima</first_name>
				<middle_name></middle_name>
				<last_name>Seifabadi</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>simaseyfabadi@yahoo.com</email>
				<code>15168</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author></author_list>
				</article>
			</articleset>
			</journal>