<?xml version="1.0" encoding="utf-8"?>
			<journal>
			<title>Avicenna Journal of Phytomedicine</title>
			<title_fa></title_fa>
			<short_title></short_title>
			<subject>Medical Sciences</subject>
			<web_url>https://ajp.mums.ac.ir/</web_url>
			<journal_hbi_system_id>0</journal_hbi_system_id>
			<journal_hbi_system_user></journal_hbi_system_user>
			<journal_id_issn>2228-7930</journal_id_issn>
			<journal_id_issn_online>2228-7949</journal_id_issn_online>
			<journal_id_pii></journal_id_pii>
			<journal_id_doi></journal_id_doi>
			<journal_id_iranmedex></journal_id_iranmedex>
			<journal_id_magiran></journal_id_magiran>
			<journal_id_sid></journal_id_sid>
			<journal_id_nlai></journal_id_nlai>
			<journal_id_science></journal_id_science>
			<language>en</language>
			<pubdate>
				<type>jalali</type>
				<year>0</year>
				<month>0</month>
				<day>1</day>
			</pubdate>
			<pubdate>
				<type>gregorian</type>
				<year>2021</year>
				<month>11</month>
				<day>1</day>
			</pubdate>
			<volume>11</volume>
			<number>6</number>
			<publish_type>online</publish_type>
			<publish_edition>1</publish_edition>
			<article_type>fulltext</article_type>
			<articleset><article>
				<language>en</language>
				<article_id_issn></article_id_issn>
				<article_id_issn_online></article_id_issn_online>
				<article_id_pubmed></article_id_pubmed>
				<article_id_pii></article_id_pii>
				<article_id_doi></article_id_doi>
				<article_id_iranmedex></article_id_iranmedex>
				<article_id_magiran></article_id_magiran>
				<article_id_sid></article_id_sid>
				<title_fa></title_fa>
				<title>β-sitosterol induces reactive oxygen species-mediated apoptosis in human hepatocellular carcinoma cell line</title>
				<subject_fa></subject_fa>
				<subject></subject>
				<content_type_fa></content_type_fa>
				<content_type>Short communication</content_type>
				<abstract_fa><![CDATA[]]></abstract_fa>
				<abstract><![CDATA[Objective: It is of interest to investigate the anti-proliferative effect of β-sitosterol (BS) on human hepatocellular carcinoma (HepG2) cell line.Materials and Methods: β-sitosterol treatments (0.6 and 1.2 mM/ml) were done in HepG2 and after 24 hr, cell viability was evaluated by MTT assay. Reactive oxygen species (ROS) accumulating potential of BS was assessed by dichloro-dihydro-fluorescein diacetate staining. Morphology related to apoptosis was investigated by acridine orange and ethidium bromide dual staining. Cytochrome c and caspase 3 expressions were evaluated by immunofluorescence and western blot analyses.Results: β-sitosterol induced cytotoxicity (p&lt;0.001) and intracellular ROS in HepG2 cells in a dose-dependent manner.BS treatments accumulated induced intracellular ROS accumulation which led to membrane damage and mitochondrial toxicity. At the molecular level, BS treatments induced cytochrome c release from mitochondria and enhanced the protein expressions (p&lt;0.05 vs 0.6 mM/ml and p&lt;0.001 vs 1.2 mM/ml) of both caspase 3 and cleaved caspase 3.Conclusion: β-sitosterol induced ROS accumulation which plays a critical role in apoptosis via the intrinsic pathway in HepG2 cells. The present investigation paves the way for further in vivo studies.]]></abstract>
				<keyword_fa></keyword_fa>
				<keyword>Liver cancer, β-sitosterol, Reactive Oxygen Species, Apoptosis, Caspase</keyword>
				<start_page>541</start_page>
				<end_page>550</end_page>
				<web_url>https://ajp.mums.ac.ir/article_17746.html</web_url>
			<author_list><author>
				<first_name>Devaraj</first_name>
				<middle_name></middle_name>
				<last_name>Ezhilarasan</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>ezhild@gmail.com</email>
				<code>76190</code>
				<coreauthor>Yes</coreauthor>
				<affiliation>Department of Pharmacology, The Blue Lab, Molecular Medicine and Toxicology Division, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author><author>
				<first_name>Mary</first_name>
				<middle_name>J</middle_name>
				<last_name>Ditty</last_name>
				<suffix></suffix>
				<first_name_fa></first_name_fa>
				<middle_name_fa></middle_name_fa>
				<last_name_fa></last_name_fa>
				<suffix_fa></suffix_fa>
				<email>dittyjmary@gmail.com</email>
				<code>76191</code>
				<coreauthor>No</coreauthor>
				<affiliation>Department of Pharmacology, The Blue Lab, Molecular Medicine and Toxicology Division, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India</affiliation>
				<affiliation_fa></affiliation_fa>
				 </author></author_list>
				</article>
			</articleset>
			</journal>