TY - JOUR ID - 7002 TI - Ginger extract modulates the expression of IL-12 and TGF-β in the central nervous system and serum of mice with experimental autoimmune encephalomyelitis JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Jafarzadeh, Abdollah AU - Ahangar-Parvin, Rayhane AU - Nemati, Maryam AU - Taghipour, Zahra AU - Shamsizadeh, Ali AU - Ayoobi, Fatemeh AU - Hassan, Zuhair Mohammad AD - Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran AD - Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran AD - Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran AD - Department of Histology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran AD - Department of Physiology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran AD - Department of Immunology, Medical School, Tarbiat Moddares University, Tehran, Iran Y1 - 2017 PY - 2017 VL - 7 IS - 1 SP - 54 EP - 65 KW - Experimental autoimmune encephalomyelitis KW - ginger KW - IL-12 KW - TGF-β KW - Serum DO - 10.22038/ajp.2016.7002 N2 - Objective: The main function of IL-12 is differentiation of naive T cells intoTh1 cells and TGF-β is a powerful immunoregulatory cytokine. The immunomodulatory and anti-inflammatory properties of ginger have also been reported in some studies. The aim of this study was to evaluate the effects of ginger extract on the expression of IL-12 and TGF-β in a model of experimental autoimmune encephalomyelitis (EAE).Materials and Methods: EAE was induced in C57BL/6 mice by immunization with myelin oligodendroglial glycoprotein emulsified in complete Freund's adjuvant. The mice were administered intra-peritoneally with ginger extracts or PBS, from day +3 to +30. On day 31, mice were scarified and the expression of IL-12 and TGF-β mRNA in the spinal cord were determined by using real time-PCR. The serum levels of cytokines were measured by ELISA.Results: In PBS-treated EAE mice, the expression of IL-12 P35 and IL-12 P40 mRNA in the CNS and the mean serum levels of IL-12 were significantly higher than those of healthy group (p<0.001). In ginger-treated EAE mice, the expression of IL-12 mRNA and its serum levels were significantly lower as compared to PBS-treated EAE mice. No significant difference was observed between PBS-treated EAE mice and healthy group regarding the expression of TGF-β mRNA. In ginger (300 mg/kg)-treated EAE group, the expression of TGF-β mRNA and its serum levels were significantly higher in comparison to PBS-treated EAE mice (p Conclusion: These results indicated that ginger extract modulates the expression of IL-12 and TGF-β in CNS and serum of EAE mice. UR - https://ajp.mums.ac.ir/article_7002.html L1 - https://ajp.mums.ac.ir/article_7002_50da80e49bdef5d61dd0775c35f6c009.pdf ER -