TY - JOUR ID - 6760 TI - Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Lodhi, Santram AU - Jain, Avijeet AU - Jain, Alok Pal AU - Pawar, Rajesh AU - Singhai, Abhay Kumar AD - Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, Madhya Pradesh, India AD - Department of Pharmacy RKDF University, Bhopal Madhya Pradesh, India-462033 AD - Department of Pharmacy, Guru Ramdas Khalsa Institute of Science & Technology, Kukrikheda, Barela, Jabalpur (M.P.), India AD - Department of Pharmacognosy, VNS Institute of Pharmacy, Bhopal, Madhya Pradesh, India AD - Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, M.P., 470003. India Y1 - 2016 PY - 2016 VL - 6 IS - 5 SP - 578 EP - 591 KW - Martynia annua KW - Tephrosia purpurea KW - Povidone Iodine ointment KW - Burn wound KW - Luteolin KW - Dead space wound DO - 10.22038/ajp.2016.6760 N2 - Objective: Martynia annua L. (M. annua), (Martyniaccae) has been traditionally used in the treatment of epilepsy, sore throat and inflammatory disorders. The leaf paste is used topically on Tuberculosis of the lymphatic glands and wounds of domestic animals. Tephrosia purpurea (T. purpurea), (Fabaceae) has been used traditionally as a remedy for asthma, gonorrhea, rheumatism and ulcers. This study aimed to evaluate the potential wound healing effects of different fractions ofethanol extract of M. annua leaves and aerial parts of T. purpurea. Materials and Methods: Methanol fraction of M. annua (MAF-C) and ethyl acetate fraction of T. purpurea (TPF-A) were evaluated for healing potential in dead-space and burn wound models. An ointment (5% w/w) of MAF-C and TPF-A, pongamol (0.2 and 0.5% w/w) and luteolin (0.2 and 0.5% w/w) was applied topically twice a day. The effects were compared with Povidone Iodine ointment with respect to protein, collagen content, enzymatic assay and histopathological finding of granuloma tissues. Results: Ethanol extracts of M. annua and T. purpureawere exhibited total flavonoid contents of 126.2 ± 4.69 and 171.6 ± 6.38 mg (quercetin equivalent), respectively. HPLC fingerprinting confirmed the presence of luteolin in M. annua and quercetin in T. purpurea. TPF-A and MAF-C ointments (5% w/w) significantly increases the hydroxyproline and protein contents. Luteolin and pongamol ointments were also found to be effective in both wound models. Conclusion: Our findings suggested that 5% w/w ointment of TPF-A and MAF-C fractions were more effective than isolated flavonoids in wound healing which may be due to synergistic interactions between the flavonoids and other constituents. UR - https://ajp.mums.ac.ir/article_6760.html L1 - https://ajp.mums.ac.ir/article_6760_7a4e912759a2aa5734383791db4a4ac0.pdf ER -