TY - JOUR ID - 4622 TI - Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Fallahi, Maryam AU - Keyhanmanesh, Rana AU - Khamaneh, Amir Mahdi AU - Ebrahimi Saadatlou, Mohammad Ali AU - Saadat, Saeideh AU - Ebrahimi, Hadi AD - Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of basic sciences, College of veterinary medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran AD - Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran AD - Tuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2016 PY - 2016 VL - 6 IS - 1 SP - 77 EP - 85 KW - Alpha-hederin KW - MiRNA-126 KW - IL-13 mRNA KW - Ovalbumin KW - Thymoquinone KW - Asthma DO - 10.22038/ajp.2016.4622 N2 - Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed. Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses. UR - https://ajp.mums.ac.ir/article_4622.html L1 - https://ajp.mums.ac.ir/article_4622_eb1e17b7fc41beaaceda6589f909135c.pdf ER -