TY - JOUR ID - 18930 TI - Sol-gel synthesis of amorphous calcium phosphate nanoparticles in brown rice substrate and assessment of their cytotoxicity and antimicrobial activities JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Beigoli, Sima AU - Hekmat, Azadeh AU - Farzanegan, Fahimeh AU - Darroudi, Majid AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Department of Orthodontics, Oral & Maxillofacial Diseases Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2022 PY - 2022 VL - 12 IS - 1 SP - 77 EP - 88 KW - Sol-gel synthesis KW - Amorphous calcium phosphate (ACP) KW - Nano-biomaterials KW - Antibacterial activity, Cytotoxicity DO - 10.22038/ajp.2021.18930 N2 - Objective: This study intended to perform a synthesizing procedure for amorphous calcium phosphate (ACP) through a green template by the usage of brown rice (BR).Materials and Methods: ACP nanoparticles were obtained by application of a sol-gel method and comprehensively characterized using X-ray powder diffraction (XRD), zeta potential, fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscope (FESEM), and atomic force microscopy (AFM). Cytotoxic activity of ACP was evaluated in human epithelial type 2 (HEp-2) cell lines. The antibacterial effects of nanoparticles were appraised against Gram-positive Streptococcus mutans and Enterococcus faecalis.Results: The procedures for the evaluation of the characterization outcomes, dispersion, and stability of our product were confirmed by observing the smooth and uniformed surfaces of ACP. The zeta potential value of the synthesized sample was -22 mV, which indicates its acceptable stable condition caused by electrostatic repulsion. The cytotoxicity of the ACP nanoparticles was investigated in HEp-2 cells, and results showed no cytotoxicity for the synthesized nanoparticles. Also, the obtained minimum inhibitory concentration (MIC) of ACP nanoparticles in opposition to S. mutans and E. faecalis was 15 and 20 µg/ml, respectively, indicating the resistance of E. faecalis in comparison to S. mutans and MBC for synthesized nanoparticles against S. mutans and E. faecalis strains was 20 and 25 µg/ml.Conclusion: The present study showed that this compound has no toxicity on the examined cell line. Also, the antibacterial properties of the synthesized ACP were approved by the obtained data, which enables the application of this material for therapeutic purposes in the pharmaceutical industry. UR - https://ajp.mums.ac.ir/article_18930.html L1 - https://ajp.mums.ac.ir/article_18930_12426d4b0626bbc81c72a9a4cf10cf95.pdf ER -