ORIGINAL_ARTICLE
The effectiveness and safety of Iranian herbal medicines for treatment of premenstrual syndrome: A systematic review
Objective: Premenstrual syndrome (PMS) is one of the most common problems among women of reproductive age. The popularity of complementary/alternative therapies has grown in recent years, and these treatments have been more commonly used by women (48.9%) than men (37.8%). The aim of this systematic review was to assess effectiveness and safety of Iranian herbal medicines for treatment of premenstrual syndrome. Methods: PubMed, Scopus, Cochrane, and Google Scholar were searched along with SID, Magiran and Irandoc up to Dec 2017. Inclusion criteria consist of Iranian, published, randomized controlled trials (RCTs) using Iranian herbal medicine for treatment of reproductive age women with PMS. Eventually Eighteen RCTs met the inclusion criteria. Results: Overall, studies have shown that Vitex agnuscastus, Hypericum perforatum, Matricaria chamomilla, saffron, Curcumin, Melissa officinalis, Zataria multiflora,Wheat Germ Extract, Echinophora platyloba, Foeniculum vulgare, Valerian root extract, Citrus sinensis, Zingiber officinale andFlax seed might alleviate symptoms of PMS. Conclusion: This research demonstrated efficacy and safety of Iranian herbal medicines in alleviating PMS. Therefore, herbal medicine can be regarded as an alternative treatment for women suffering from PMS.
https://ajp.mums.ac.ir/article_10204_ec3828dc3b7e0408c7a8746e635c9b37.pdf
2018-03-01
96
113
10.22038/ajp.2018.22810.1848
Herbal medicines
Premenstrual Syndrome
Systematic review
Nahid
Maleki-Saghooni
malekisn931@mums.ac.ir
1
Student Research Committee, Department of Midwifery, School of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Fatemeh Zahra
Karimi
mastermid63@gmail.com
2
Department of Midwifery and Reproductive Health, Nursing and Midwifery School, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Zahra
Behboodi Moghadam
behboodi@tums.ac.ir
3
Department of Midwifery and Reproductive Health, Nursing and Midwifery School, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Khadigeh
Mirzaii Najmabadi
mirzaiikh@google.com
4
Department of Midwifery and Reproductive Health, Nursing and Midwifery School, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Adib-Hajbaghery M. and Hoseinian M. 2014. Knowledge, attitude and practice toward complementary and traditional medicine among Kashan health care staff. Complement Ther Med, 22, 126-132.
1
Agha‐Hosseini M, Kashani L, Aleyaseen A, Ghoreishi A, Rahmanpour H, Zarrinara A.R. and Akhondzadeh S. 2008. Crocus sativus L.(saffron) in the treatment of premenstrual syndrome: a double‐blind, randomised and placebo‐controlled trial. BJOG, 115, 515-519.
2
Akbarzadeh M, Dehghani M, Moshfeghy Z, Emamghoreishi M., Tavakoli P and Zare N. 2015. Effect of Melissa officinalis capsule on the intensity of premenstrual syndrome symptoms in high school girl students. NMSJ, 4:2.
3
Ghasemi dehkordi et al. 2003. "Iranian Herbal Pharmacopoeia." HHSRJ 6: 63-70.
4
Andrus, G.M., 2001. Recent and future advances in the treatment of PMS, PMD, and menopause. IDrugs, 4, 1373-1381.
5
Ataollahi, M, Akbari S, Mojab F and Majd H.A. 2015. "The effect of wheat germ extract on premenstrual syndrome symptoms." IJPR, 14: 159-166.
6
Beiranvand S.P, Beiranvand N.S, Moghadam, Z.B, Birjandi M, Azhari S, Rezaei E, Salehnia A.N. and Beiranvand S. 2016. "The effect of Crocus sativus (saffron) on the severity of premenstrual syndrome. Eur J Integr Med, 8:55-61
7
Bertone-Johnson E.R, Hankinson S.E, Willett W.C, Johnson S.R and Manson, J.E. 2010. "Adiposity and the development of premenstrual syndrome." Res J Women's Health, 19: 1955-1962.
8
Beidokhti H, Heidari M and Firozeh M. 2013. The Soy Bean Effect on the Premenstrual Syndrome Concerning Its Physical٬ Psycho and Behavioral Effects in Female Students of Semnan University Dormitory. J. Urmia Nurs. Midwifery Fac. 11: 316-323 (in Persian).
9
Brown J, O’Brien P.M, Marjoribanks J and Wyatt K. 2009. Selective serotonin reuptake inhibitors for premenstrual syndrome. Cochrane Database Syst Rev. 15: 2.
10
Del Giorno C, Da Fonseca A.M, Bagnoli V.R, de Assis J.S, Soares Jr J.M and Baracat E.C. 2010. "Effects of Trifolium pratense on the climacteric and sexual symptoms in postmenopausal women. Rev Assoc Med Bras 56: 558-562.
11
Delaram M., 2014. "Treatment of moderate to severe of premenstrual syndrome with echinophora platyloba. Zahedan J Res Med Sci, 16 : 50-54.
12
Delaram M and Heydarnejad M.S. 2011. Herbal Remedy for Premenstrual Syndrome with Fennel (Foeniculum vulgare)–Randomized, Placebo-Controlled Study; 55:57-3
13
Dickerson L.M, Mazyck P.J and Hunter M.H, 2003. "Premenstrual syndrome. Am Fam Physician, 67: 1743-1752.
14
Dimmock P.W, Wyatt K.M, Jones P.W and O'brien P.S.2000. Efficacy of selective serotonin-reuptake inhibitors in premenstrual syndrome: a systematic review. The Lancet, 356: 1131-1136.
15
Domoney C.L, Vashisht A and Studd, J.W.W. 2003. "Premenstrual syndrome and the use of alternative therapies. Ann. N. Y. Acad. Sci. 997: 330-340.
16
Douglas S. 2002. "Premenstrual syndrome. Evidence-based treatment in family practice. Can. Fam. Physician, 48: 1789-1797.
17
Dworkin R.H, Turk D.C, Peirce-Sandner S, Baron R, Bellamy N, Burke L.B.Chappell A, Chartier K, Cleeland C.S, Costello A, and Cowan P.2010. Research design considerations for confirmatory chronic pain clinical trials: IMMPACT recommendations. PAIN®, 149: 177-193.
18
Easterbrook P.J, Gopalan R, Berlin J.A and Matthews D.R. 1991. Publication bias in clinical research. The Lancet, 337: 867-872.
19
Emtiazy M., Keshavarz M, Khodadoost M, Kamalinejad M, Gooshahgir S.A, Bajestani H.S, Dabbaghian F.H and Alizad, M. 2012. Relation between body humors and hypercholesterolemia: an Iranian traditional medicine perspective based on the teaching of Avicenna. Iran Red Crescent Med J 2012; 14:133–8.
20
Ernst E and Pittler M.H. 1997. Alternative therapy bias. Nature, 385: 480.
21
Fallah Huseini H, Fakhrzadeh H, Larijani B and Shikh Samani, A.H. 2006. "Review of anti-diabetic medicinal plant used in traditional medicine." J. Med. Plants. 1: 1-8.
22
Ford O, Lethaby A, Roberts H and Mol, B.W.J.2012. Progesterone for premenstrual syndrome. The Cochrane Library.
23
Forrester-Knauss C, Stutz E.Z, Weiss C and Tschudin S.2011. "The interrelation between premenstrual syndrome and major depression: results from a population-based sample." BMC public health 11: 795.
24
Girman A, Lee R and Kligler B.2003. "An integrative medicine approach to premenstrual syndrome. Am J Obstet Gynecol, 188: 56-65.
25
Graham S. 1995. "Narrative versus meta-analytic reviews of race differences in motivation: A comment on Cooper and Dorr. Rev. Educ. Res, 65: 509-514.
26
Halbreich U, Borenstein J, Pearlstein T and Kahn L.S.2003. "The prevalence, impairment, impact, and burden of premenstrual dysphoric disorder (PMS/PMDD).Psych neuro endocrinology 28: 1-23.
27
Henshaw C.A. 2007. "PMS: diagnosis, aetiology, assessment and management. Adv Psychiatr Treat 13: 139-146.
28
Hur M.H, Lee M.S, Seong K.Y and Lee M.K. 2012. "Aromatherapy massage on the abdomen for alleviating menstrual pain in high school girls: a preliminary controlled clinical study. Evid. Based Complement. Alternat. Med.
29
Kamranpur B, Farzadrik L, Rahbar T and Alizade S. 2015. "Valerian impact on the severity of the symptoms of premenstrual syndrome” IJOGI 18: 1-9(in Persian).
30
Kessel B., 2000. "Premenstrual syndrome: Advances in diagnosis and treatment. Obstet Gynecol Clin North Am. 27: 625-639.
31
Khaiat S, Kheirkhah M, Behboodi-Moghadam Z, Fanaee H and Kasaiian A .2014. "Ginger impact on the symptoms of premenstrual syndrome in female students with PMS. Medsurg Nurs. 3: 10-16 (in Persian).
32
Khayat S, Fanaei H, Kheirkhah M, Moghadam Z.B, Kasaeian A and Javadimehr M. 2015. "Curcumin attenuates severity of premenstrual syndrome symptoms: A randomized, double-blind, placebo-controlled trial. Complement. Ther. Med, 23: 318-324.
33
KHeirkhah M, Abassinia K, Jahdi F, Hosseini, F. and Hassani M. 2013. "The effect of perforan on the mood symptoms of premenstrual syndrome. J. Urmia Nurs. Midwifery Fac, 11: 245-251(in Persian).
34
Kiani A.A, Heydari M, Mohammadi T.S And Faghihzadeh S.2009. Prevalence, signs, symptoms and predisposing factors of premenstrual syndromes in employed women, 45-54.
35
Lentz G.M, Lobo R.A., Gershenson D.M. and Katz V.L. 2012. Comprehensive Gynecology, 6th ed, Philadelphia: Elsevier Inc.
36
Masho S.W, Adera T and South-Paul J. 2005. "Obesity as a risk factor for premenstrual syndrome. J Psychosom Obstet Gynaecol, 26: 33-39.
37
Menati L, Khaleghinezhad K, Tadayon M. and Siahpoosh A. 2014. "Evaluation of contextual and demographic factors on licorice effects on reducing hot flashes in postmenopause women. Health Care Women Int 35: 87-99.
38
Mirghafourvand M, Mohammad Alizadeh Charandabi S, Javadzadeh Y and Ahmadpour P. 2015. "Comparing the Effects of Vitexagnus and Flaxseed on Premenstrual symptoms: a Randomized Controlled Trial." Journal of hayat, 21:68-78. (in Persian).
39
Mishell Jr, D.R. 2005. "Premenstrual disorders: epidemiology and disease burden." Am J Manag Care 11: 473-479.
40
Moerman D.E. 2000."Cultural variations in the placebo effect: ulcers, anxiety, and blood pressure." MAQ, 14: 51-72.
41
Behboodi Moghadam Z, Kheirkhah M, Golian.Tehrani S, Ghudarzi S, Haghani H and Shiroodgholami R. 2014. "The impact of Valerian root extract on mood and behavioral symptoms severity in premenstrual syndrome. Medsurg Nurs. 3: 71-76 (in Persian).
42
Moline M.L and Zendell, S.M. 2000. "Evaluating and managing premenstrual syndrome. J Womens Health, 5: 1-1.
43
Moosavi P, H. Zaheri S, Najjar P, Afshari and Haiati F. 2014. "The effect of Vitex on premenstrual syndrome. IJOGI, 17: 1-9(in Persian).
44
Ozgoli G, Shahve M, Ismaili S, and Oskouei N. 2012. "The effect of orally oil of orange peel on the severity of symptoms of premenstrual syndrome, double-blind, placebo-controlled clinical trial. J Reprod Infertil, 12: 123-129 (in Persian).
45
PakGohar M, SalehiSurmaq M H, Mehran A and Akhondzadeh S. 2006. "Effect of Hypericum perforatum L. in the treatment of premenstrual syndrome. J. Med. Plants Res, 4: 33-42(in Persian).
46
Pinar G, Colak M, Oksuz E. 2011. Premenstrual Syndrome in Turkish college students and its effects on life quality. Sex Reprod Healthc, 2:21-7
47
Rapkin A. 2003. A review of treatment of premenstrual syndrome & premenstrual dysphoric disorder. Psychoneuroendocrinology, 28: 39-53.
48
Rocha Filho EA, Lima JC, Pinho Neto JS, Montarroyos U. 2011. Essential fatty acids for premenstrual syndrome and their effect on prolactin and total cholesterol levels: a randomized, double blind, placebo-controlled study. Reprod Health 8:2.
49
Saki M, Akbari S, Saki M, Tarrahi M J, Gholami M, Pirdadeh S. 2015. The effect of primrose oil on the premenstrual syndrome among the female students in Lorestan University of Medical Sciences: A triple blind study. JNMS, 2:20-26.
50
Samadi Z, Taghian F, Valiani M. 2013. The effects of 8 weeks of regular aerobic exercise on the symptoms of premenstrual syndrome in non‑athlete girls. Iran J Nurs Midwifery Res, 18:14-9.
51
Schiola A , Lowin J, Lindemann M, Patel R, Endicott J. 2011. The burden of moderate/severe premenstrual syndrome and premenstrual dysphoric disorder in a cohort of Latin American women. Value Health, 14: S93-S95.
52
Sharifi F,Simbar M, Mojab F, Majd HA. 2014. "Comparison of the effects of Matricaria chamomila (Chamomile) extract and mefenamic acid on the intensity of premenstrual syndrome. Complement Ther Clin Pract, 20: 81-88.
53
Sodouri M, Masoudi Alavi N, Fathizadeh N, Taghizadeh M, Azarbad Z, Memarzadeh M. 2013. Effects of Zataria Multi-Flora, Shirazi thyme, on the Severity of Premenstrual Syndrome. Nurs Midwifery Stud, 2: 57.
54
Soltan Ahmadi J, Zad-Kafi F, Nikian Y, Yasemi MT. 2007. The prevalence and severity of premenstrual syndrome in students of Kerman University of Medical Sciences. J Yasuj Univ Med Sci, 2:16–23.
55
Fritz MA, Speroff L. 2011. Clinical gynecologic endocrinology and infertility. lippincott Williams & wilkins. Translated by R Ghotbi, B Ghazi and MD Jahani Tehran, Golban Pub(Persian). 2011
56
Steiner M, T. Pearlstein T, Cohen LS, Endicott J, Kornstein SG, Roberts C, Roberts DL, Yonkers K. 2006. Expert guidelines for the treatment of severe PMS, PMDD, and comorbidities: the role of SSRIs. J Womens Health (Larchmt), 15: 57-69.
57
Amasha HA, Mohamed RA, Nageeb H. 2017. Complementary and alternative therapies for premenstrual syndrome: an exploratory study. Khartoum Medical Journal, 10:7-15.
58
Taavoni S, Barkhordari F, Goushegir A, Haghani H. 2014. Effect of Royal Jelly on premenstrual syndrome among Iranian medical sciences students: A randomized, triple-blind, placebo-controlled study. Complement Ther Med, 22: 601-6.
59
Tolossa FW, Bekele ML. 2014. Prevalence, impacts and medical managements of premenstrual syndrome among female students: cross-sectional study in college of health sciences, Mekelle University, Mekelle, northern Ethiopia. BMC Womens Health, 14:52.
60
Vishnupriya R, Rajarajeswaram P. 2011. "Effects of aerobic exercise at different intensities in premenstrual syndrome. J Obstet Gynaecol India, 61: 675-682.
61
Weisz G, Knaapen L. 2009. Diagnosing and treating premenstrual syndrome in five western nations. Soc Sci Med, 68: 1498-505.
62
Wong C, Lai KY, Tse HM. 2010. Effects of SP6 acupressure on pain and menstrual distress in young women with dysmenorrhea. Complement Ther Clin Pract, 16: 64-69.
63
WyattKM, Dimmock PW, Frischer M, Jones PW, O'Brien SP. 2002. Prescribing patterns in premenstrual syndrome. BMC Womens Health, 2: 4.
64
Younesi A, Lary R. 2015. Medicinal plants and their impact on male infertility. The First International Congress of reproduction.
65
Zamani M, Neghab N, Torabian S. 2012. Therapeutic effect of Vitex agnus castus in patients with premenstrual syndrome. Acta Med Iran, 50: 101-6.
66
ZomorodianK, Saharkhiz MJ, Rahimi MJ, Bandegi A, Shekarkhar G, Bandegani A, Pakshir K, Bazargani A. 2011. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Pharmacogn Mag, 7: 53-9.
67
Karimi A, Majlesi M, Rafieian-Kopaei M. 2015. Herbal versus synthetic drugs; beliefs and facts. J Nephropharmacol, 4:27-30.
68
ORIGINAL_ARTICLE
The field efficacy of Nigella sativa and Berberis vulgaris methanolic extracts against Haemoproteus columbae
Objective: The methanolic extracts of Nigella sativaL. seeds (MENS) and Berberis vulgaris L.(MEBV) were investigated for treatment of Haemoproteus columbae-infected pigeons (Columba livia domestica). Materials and Methods: One hundred twenty naturally-infected pigeons were randomly divided into four groups of thirty each. Two groups were treated separately with the extracts, while the positive and negative control groups were given buparvaquone (Butalex®) and distilled water, respectively. The parasitaemia rate was calculated in all groups before and after the experiment at four-day intervals for16 days. Results: The results showed a high therapeutic effect for MENS with a progressive decrease in average parasitaemia rate from 18.17% before treatment to 0.73% at the end of treatment (p<0.05), while Butalex® was able to suppress the parasitemia rate from 18.90% before treatment to 0.23% at the end of experiment (p<0.05). However, no significant changes in parasitemia rate were evident in groups treated with MEBV (p>0.05). Conclusion: Methanolic extracts of N. sativa showed therapeutic effects against H. columbae and may be regarded as a suitable choice for further studies to develop new drugs against blood parasites, in both animals and human beings.
https://ajp.mums.ac.ir/article_9880_f1482e354275f9daf384d445a5d802fe.pdf
2018-03-01
114
121
10.22038/ajp.2017.18152.1707
Nigella Sativa
Berberis vulgaris
Haemoproteus columbae
Pigeon
Mostafa
Razavi
mrazavi@shirazu.ac.ir
1
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
Mohammad
Asadpour
asadpour_m@shirazu.ac.ir
2
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Hossein
Malekpour
pmaleki2000@yahoo.com
3
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Arash
Jafari
arash.jafari87@gmail.com
4
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed, 3: 337-352.
1
Al-Naggar T, Gomez-Serranillos M, Carretero M, Villar A. 2003. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol, 88: 63-68.
2
Ashford Rw. 1971. Blood parasites and migratory fat at Lake Chad. 113, pp.100-101, Ibis, John Wiley & Sons.
3
Atkinson CT, Dusek RJ, Woods KL, Iko WM. 2000. Pathogenicity of avian malaria in experimentally-infected Hawaii Amakihi. J Wildl Dis, 36: 197-201.
4
Bishopp F. 1929. The pigeon fly-an important pest of pigeons in the United States. J Econ Entomol, 22: 974-980.
5
Cardona CJ, Ihejirika A, McClellan L. 2002. Haemoproteus lophortyx infection in bobwhite quail. Avian Dis, 46: 249-255.
6
Cheesman S. 2000. The topoisomerases of protozoan parasites. Parasitol Today, 16: 277-281.
7
El-Metenawy T. 1999. Therapeutic effects of some antihaematozoal drugs against Haemoproteus columbae in domestic pigeons. DtschTierarztlWochenschr, 106: 72-72.
8
El-Shabrawy O, Nada S. 1996. Biological evaluation of multicomponent tea used as hypoglycemic in rats. Fitoterapia, 67: 99-102.
9
El Zalabani SM, El-Askary HI, Mousa OM, Issa MY, Zaitoun AA, Abdel-Sattar E. 2012. Acaricidal activity of Swieteniam ahogani and Swieteniam acrophylla ethanolic extracts against Varroa destructor in honeybee colonies. Exp Parasitol, 130: 166-170.
10
Fata A, Rakhshandeh H, Berenji F, Jalilianfard A. 2006. Treatment of Cutaneous Leishmaniasis in murine model by Alcoholic Extract of Berberis vulgaris. Iran J Parasitol, 1: 39-42.
11
Garvin MC, Homer BL, Greiner EC. 2003. Pathogenicity of Haemoproteus danilewskyi, Kruse, 1890, in blue jays (Cyanocitta cristata). J Wildl Dis, 39: 161-169.
12
Gessler M, Nkunya MH, Mwasumbi LB, Heinrich M, Tanner M. 1994. Screening Tanzanian medicinal plants for antimalarial activity. Acta Trop, 56: 65-77.
13
Ishtiaq F, Gering E, Rappole JH, Rahmani AR, Jhala YV, Dove CJ, Milensky C, Olson SL, Peirce MA, Fleischer RC. 2007. Prevalence and diversity of avian hematozoan parasites in Asia: a regional survey. J Wildl Dis, 43: 382-398.
14
Khan N, Sultana S. 2005. Inhibition of two stage renal carcinogenesis, oxidative damage and hyper proliferative response by Nigella sativa.Eur J Cancer Prev, 14: 159-168.
15
Knowles S, Palinauskas V, Sheldon B. 2010. Chronic malaria infections increase family inequalities and reduce parental fitness: experimental evidence from a wild bird population. J Evol Biol, 23: 557-569.
16
Mahmoud M, El-Abhar H, Saleh S. 2002. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol, 79: 1-11.
17
Mahmoudvand H, Sharififar F, Rahmat MS, Tavakoli R, Dezaki ES, Jahanbakhsh S, Sharifi I. 2014. Evaluation of anti-leishmanial activity and cytotoxicity of the extracts of Berberis vulgaris and Nigella sativa against Leishmania tropica. J Vector Borne Dis, 51: 294.
18
Majdalawieh AF, Hmaidan R, Carr RI. 2010. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol, 131: 268-275.
19
Manwell RD, Loeffler CA. 1961. Glucose consumption by Haemoproteus columbae. J Parasitol, 47: 285-290.
20
Martinsen ES, Perkins SL, Schall JJ. 2008. A three-genome phylogeny of malaria parasites (Plasmodium and closely related genera): evolution of life-history traits and host switches. Mol Phylogenet Evol, 47: 261-273.
21
Moazeni M, Nazer A. 2010. In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World J Surg, 34: 2677-2681.
22
Møller AP, Nielsen JT. 2007. Malaria and risk of predation: a comparative study of birds. Ecology, 88: 871-881.
23
Muregi F, Chhabra S, Njagi E, Lang'at-Thoruwa C, Njue W, Orago A, Omar S, Ndiege I. 2003. In vitro antiplasmodial activity of some plants used in Kisii, Kenya against malaria and their chloroquine potentiation effects. J Ethnopharmacol, 84: 235-239.
24
Nasir A, Avais M, Khan M, Khan J, Hameed S, Reichel M. 2013. Treating Cryptosporidium parvum infection in calves. J Parasitol, 99: 715-717.
25
O'roke EC. 1930. The Morphology, Transmission, and Life-history of Haemoproteus lophortyxO'Roke, a Blood Parasite of the California Valley Quail, Univ California Pub Zool, 36: 1-50.
26
Okeola VO, Adaramoye OA, Nneji CM, Falade CO, Farombi EO, Ademowo OG. 2011. Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis. Parasitol Res, 108: 1507-1512.
27
Rahman NNNA, Furuta T, Takane K, Mohd MA. 1999. Antimalarial activity of extracts of Malaysian medicinal plants. J Ethnopharmacol, 64: 249-254.
28
Razavi SM, Asadpour M, Jafari A, Malekpour SH. 2015. The field efficacy of Lepidium latifolium and Zataria multiflora methanolic extracts against Varroa destructor. Parasitol Res, 114: 4233-4238.
29
Ricklefs RE, Fallon SM, Bermingham E. 2004. Evolutionary relationships, cospeciation, and host switching in avian malaria parasites. Syst Biol, 53: 111-119.
30
Ricklefs RE, Swanson BL, Fallon SM, MartÍnez-AbraÍn A, Scheuerlein A, Gray J, Latta SC. 2005. Community relationships of avian malaria parasites in southern Missouri. Ecol Monogr, 75: 543-559.
31
Rodrigues JR, Gamboa ND. 2009. Effect of dequalinium on the oxidative stress in Plasmodium berghei-infected erythrocytes. Parasitol Res, 104: 1491-1496.
32
Salehabadi A, Karamian M, Farzad MH, Namaei MH. 2014. Effect of root bark extract of Berberis vulgaris L. on Leishmania major on BALB/c mice. Parasitol Res, 113: 953-957.
33
Salem ML, Hossain MS. 2000. Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection. Int J Immunopharmacol, 22: 729-740.
34
Slater LB. 2005. Malarial birds: Modeling infectious human disease in animals. Bull Hist Med, 79: 261-294.
35
Tomás G, Merino S, Moreno J, Morales J, Martinez‐De La Puente J. 2007. Impact of blood parasites on immunoglobulin level and parental effort: a medication field experiment on a wild passerine. FunctEcol, 21: 125-133.
36
Valkiunas G. 2004. Avian malaria parasites and other haemosporidia. pp. 12-16, CRC press, London, Taylor & Francis.
37
Valkiunas G, Zickus T, Shapoval AP, Iezhova TA. 2006. Effect of Haemoproteus belopolskyi (Haemosporida: Haemoproteidae) on body mass of the blackcap Sylvia atricapilla. J Parasitol, 92: 1123-1125.
38
Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. 2002. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine, 9: 69-74.
39
ORIGINAL_ARTICLE
Evaluation of cytotoxic effects and acute and chronic toxicity of aqueous extract of the seeds of Calycotome villosa (Poiret) Link (subsp. intermedia) in rodents
Objective: The present investigation was carried out to evaluate the safety of an aqueous extract of the seeds of Calycotome villosa (Poiret) Link (subsp. intermedia) by determining its cytotoxicity and potential toxicity after acute and sub-chronic administration in rodents. Materials and Methods: Cytotoxic activity was tested in cancer and non-cancer cell lines HeLa, Mel-5, HL-60 and 3T3. Acute toxicity tests were carried out in mice by a single oral administration of Calycotome seed-extract (0 - 12 g/kg) as well as intraperitoneal doses of 0 - 5 g/kg. Sub-chronic studies were conducted in Wistar rats by administration of oral daily doses for up to 90 days. Changes in body and vital organ weights, mortality, haematology, clinical biochemistry and histologic morphology were evaluated. Results: The lyophilized aqueous extract of C. villosa exhibited a low cytotoxicity in all cell lines tested with an IC50 > 100 µg/ml. In the acute study in mice, intra-peritoneal administration caused dose-dependent adverse effects and mortality with an LD50 of 4.06 ± 0.01 g/kg.In the chronic tests, neither mortality nor visible signs of lethality was seen in rats. Even AST and ALT were not affected while a significant decrease in serum glucose levels, at 300 and 600 mg/kg was detected. Histopathological examination of the kidney and liver did not show any alteration or inflammation at the end of treatment. Conclusion: In conclusion, the aqueous extract of C. villosa seed appeared to be non-toxic and did not produce mortality or clinically significant changes in the haematological and biochemical parameters in rats.
https://ajp.mums.ac.ir/article_9565_6cd264601f336f605da0aaa73df0c204.pdf
2018-03-01
122
135
10.22038/ajp.2017.23177.1859
Calycotome villosa (Link Subsp. Intermedia) seed
Cytotoxicity
Acute and chronic toxicity
Clinical Chemistry
Hematology
Badiaa
Lyoussi
lyoussi@gmail.com
1
Laboratory of Physiology-Pharmacology and Environmental Health, University Sidi Mohamed Ben Abdallah, Fez, Morocco
AUTHOR
Khadija
Cherkaoui Tangi
cher.khadija@gmail.com
2
Laboratory of Physiology-Pharmacology and Environmental Health, University Sidi Mohamed Ben Abdallah, Fez, Morocco
AUTHOR
Nicole
Morel
nicole.morel@uclouvain.be
3
Institute of Neuroscience, Université catholique de Louvain, Brussels, Belgium
AUTHOR
Mohamed
Haddad
m.haddad@uclouvain.be
4
GNOS research group, Louvain Drug Research Inistitute, Université catholique de Louvain, Brussels, Belgium
AUTHOR
Joelle
leclercq
joelle.leclercq@uclouvain.be
5
GNOS research group, Louvain Drug Research Inistitute, Université catholique de Louvain, Brussels, Belgium
AUTHOR
Akdogan M, Kilinc I, Oncu M, Karaoz E, Delibas N. 2003. Investigation of Biochemical and Histopathological Effects of Mentha piperita L and Mentha spicata L on Kidney tissue in Rats. Hum Exp Toxicol, 22: 213-219.
1
Alexeeff GV , Broadwin R , Liaw J , Dawson SV .2002. Characterization of the LOAEL-to-NOAEL uncertainty factor for mild adverse effects from acute inhalation exposures. Regul Toxicol Pharmacol, 36:96-105.
2
Aniagu SO, Nwinyi FC, AkumkaDD, AjokuGA, DzarmaS, IzebeKS, DitseM, Nwaneri, PC, WambebeC, GamanielK . 2005 . Toxicity studies in rats fed nature cure bitters. Afr J Biotechnol, 4: 72-78.
3
Avula B, Wang YH, Pawar RS, Shukla YJ, Schaneberg B, Khan IA. 2006. Determination of the appetite suppressant P57 in Hoodia gordonii plant extracts and dietary supplements by liquid chromatography/electrospray ionization mass spectrometry (LC-MSD-TOF) and LC-UV methods. J Aoac Int, 89 :606-611.
4
Baliga MS , Jagetia GC , Ulloor JN , Baliga MP , Venkatesh P , Reddy R , Rao KV, Baliga BS , Devi S , Raju SK , Veeresh V, Reddy TK, Bairy KL. 2004. The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats. Toxicol Lett, 151:317-326.
5
Cherkaoui TK, Lachkar M, Wibo M, Morel N, Gilani AH, Lyoussi B. 2008. Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats. Phytother Res, 22: 356-361.
6
Cherkaoui TK., El-Hilaly J, Israili ZH, Lyoussi B. 2009. Ethnobotanical survey and pharmacological screening of medicinal plants used as antihypertensive in Sefrou province (Middle North of Morocco): Benefits and challenges. Submitted in Journal of Ethnopharmacology
7
Dioka C, Orisakwe O.E, Afonne O.J, Agbasi P.U, Akumka DD, Okonkwo CJ, Ilondu N. 2002. Investigation into the haematologic and hepatoxic effects of rinbacin in rats. J Health Sci, 48: 393–398.
8
Drici MD, Clement N. 2001. Is gender a risk factor for adverse drug reactions? The example of drug-induced long QT Syndrome. Drug Saf, 24: 575–585.
9
Ebert, SN, Liu XK, Woosley, RL. 1998. Female gender as a risk factor for drug-induced cardiac arrhythmias: evaluation of clinical and experimental evidence. J Womens Health, 7: 547–557.
10
El Antri, A, Messouri, I, Bouktaib, M, El Alami R, Bolte, M, El Bali, B, Lachkar, M. 2004a. Isolation and X-ray crystal structure of tetrahydroisoquinoline alkaloids from Calycotome villosa Subsp. Intermedia. Molecules, 9: 650–657.
11
El Antri, A, Messouri I, Bouktaib, M, El Alami R, El Bali, B, Lachkar, M. 2004b. Isolation and x-ray crystal structure of a new isoquinoline-N-oxide alkaloid from Calycotome villosa Subsp. Intermedia. Fitoterapia, 75: 774–778.
12
El Antri, A, Messouri I, Chendid Tlemcani R , Bouktaib, M, El Alami R, El Bali, B, Lachkar, M. 2004c. Flavone glycosides from Calycotome villosa Subsp. Intermedia. Molecules 9: 568–573.
13
Gazda, V.E., Gomes-Carneiro, M.R., Barbi, N.S., Paumgartten FJR. 2006. Toxicological evaluation of an ethanolic extract from Chiococca alba roots. J Ethnopharmacol, 105: 187-195.
14
Greuter W, Burdet, HM, Long, G (eds). 1989. Med-Checklist 4: A Critical Inventory of Vascular Plants of the Circum Mediterranean Countries; Dicotyledones (LauraceaeRhamnaceae). C.B. de Genève: Geneva. Grossel SS, Crowl AD. 1994. editors. Marcel Dekker, Inc., New York.Handbook of Highly Toxic Materials Handling and Management.
15
Huang, RL, Chen, CC, Huang, YL., OU JC, Hu CP, Chen CF, Chang C. 1998. Anti- Tumor effects of d-dicentrine from the root of lindera megaphylla. Planta Med, 64: 212-215.
16
(IFCC) International Federation of Clinical Chemistry).1986. On IFCC methods for the measurement of catalytic concentration of enzymes. Part 2. IFCC method for aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1). J Clin Chem Clin Biochem, 24:497-510.
17
IFCC (International Federation of Clinical Chemistry), 1980. IFCC methods for the measurement of catalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase (l-alanine: 2-oxoglutarate aminotrasferase, EC 2.6.1.2). Clinica Chimica Acta, 105: 147F–154F.
18
Kallner A, Tryding N. 1989. IFCC Guidelines to the Evaluation of Drug Effects in Clinical Chemistry. Scandinavian Journal of Clinical and Laboratory Investigation, 49, 1–29.
19
Kennedy GL, Ferenz RL, Burgess BA. 1986. Estimation of acute oral toxicity in rats by the determination of the approximate lethal dose rather than the LD50. J Appl Toxicol, 6:145-148.
20
Kim JH, Hahm DH, Yang DC, Kim JH, Lee HJ, Shim I. 2005. Effect of crude saponin of Korean red ginseng on high-fat diet-induced obesity in the rat. J Pharmacol Sci, 97:124-31.
21
King DJ, Kelton JG.1984. Heparin – Associated Thrombocytopaenia. Ann Intern Med, 100:535-40.
22
Kouitcheu Mabeku LB, Penlap Beng V, Kouam J, Essame, O, Etoa, FX. 2007. Toxicological evaluation of ethyl acetate extract of Cylicodiscus gabunensis stem bark (Mimosaceae). J Ethnopharmacol, 111: 598–606.
23
Lee TY, Wu ML, Deng, JF, Hwang DF. 2002. High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products. J Chromatograph B: Analytical Technol Biomed Life Sci, 766:169-174.
24
Liechti ME, Gamma A, Vollenweider FX. 2001. Gender differences in the subjective effects of MDMA. Psychopharmacology, 154: 161–168.
25
Lin TJ, Su CC, Lan CK, Jiang DD, Tsai JL, Tsai MS. 2003. Acute Poisonings with Breyniaofficinalis – An Outbreak of Hepatoxicity. J Clin Toxicol, 41: 591–594.
26
Litchfield JT, Wilcoxon F.1949. A simplified method of evaluating dose effect experiments. J Pharmacol Exp Ther, 96: 99–113.
27
Lopez TA, Campero CM, Chayer R, Cosentino B, Caracino M. 1996. Experimental toxicity of verbesina encelioides in sheep and isolation of galegine. Veterin Human Toxicol, 38: 417-419.
28
Loy G, Cottiglia F, Garau D, Deidda D, Pompei R, Bonsignore L. 2001. Chemical composition and cytotoxic and antimicrobial activity of Calycotome villosa (Poiret) link leaves. Farmaco , 56: 433-436.
29
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J immunol method, 65: 55-63
30
Mukinda JT, Syce JA. 2007. Acute and chronic toxicity of the aqueous extract of Artemisia afra in rodents. J Ethnopharmacol, 112: 138–144.
31
Neese JM, Duncan P, Bayse D, Robinson M, Cooper T, Stewart C. 1976. Development and evaluation of Hexokinase/Glucose-6-Phosphat Dehydrogenase Procedure for Use as a National Glucose Reference Method. U.S. Department of HEW No. (CDC) 77-8330, 1–7.
32
Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A. 2000. Concordance of toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol,32: 56–67.
33
Raza M, Al-Shabanah OA, El-Hadiyah TM, Al-Majed AA. 2002. Effects of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swisss albino mice. Scientia Pharm, 70: 135-145.
34
Rebecca MA, Ishii-Iwamoto EL, Grespan R, Cuman RKN, Caparroz-Assef SM, Mello JCP de, Bersani-Amado CA. 2002. Toxicological studies on Stryphnodendron adstringens. J Ethnopharmacol, 83: 101-104.
35
Ross MH, Reith EJ, Romrell IJ. 1989. A text an atlas. Histology 1-2. Silva EJR, Goncalves ES, Aguiar F, Evencio LB, Lyra MMA, Coelho MCOC, Fraga MCCA, Wanderley AL. 2007. Toxicological studies on hydroalcohol extract of Calendula officinalis L. Phytother Res, 21: 332–336.
36
Stévigny C, Block S, De Pauw-Gillet MC, de Hoffmann E, Liabrès G, Adjakidjé V, Quetin-Leclercq J. 2002. Cytotoxic aporphine alkaloids from Cassytha filiformis. Planta Med, 68:1042-1044.
37
Tabacco A, Meiattini F, Moda E, Tarli P. 1979. Simplified enzymic/ colorimetric serum urea nitrogen determination. Clin Chem, 25: 336–337.
38
Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. 2002. A 90-day oral gavage toxicity study of D-methylphenidate and D, L-methylphenidate in Sprague Dawley rats. Toxicology, 179: 183-196.
39
Twaij HAA, Kery A, Al Khazraji NK. 1983. Some pharmacological, toxicological and phytochemical investigations on Centaurea phyllocephala. J Ethnopharmacol, 9: 299–314.
40
Van Miert AS. 1989. Extrapolation of pharmacological and toxicological data based on metabolic weight. Arch fur Exp Veterinarmed, 43: 481-488.
41
Waynforth BH, Flecknell PA. 1980. Injection techniques. In: Experimental and Surgical Techniques in the Rat. Academic Press, London, N.Y., USA 30–61.
42
Zhu M, Lew KT, Leung, PL. 2002. Protective effects of Plant formula on ethanolic-induced gastric lesions in rats. Phytother Res, 16: 276–280.
43
ORIGINAL_ARTICLE
In vitro and in vivo evaluations of Pelargonium roseum essential oil activity against Trichomonas gallinae
Objective: Pelargonium roseum Willd.(Geraniaceae) is widely grown as an ornamental plant due to its strong pleasant rose-like odor. The present study evaluates the antitrichomonal effect of P. roseum essential oil (EO) against Trichomonas gallinae both in vitro and in vivo and compares it to that of metronidazole (MTZ) as a standard antitrichomonal drug. Materials and Methods: In vitro assays were accomplished in multi-well plates containing MTZ and EO at final concentrations of 2.5, 5, 10, 20, 50, and 100 μg/mL. In vivo assay was carried out on 40 experimentally infected pigeons receiving MTZ and EO at doses of 25 and 50 mg/kg. Results: The 24-hr MIC of MTZ was 10 µg/mL, while for EO it was 20 µg/mL. Treatment with MTZ 50 mg/kg after 4 days led to full recovery of infected pigeons however EO 50 mg/kg resulted in the same outcome after 5 days. No mortality or clinical side effects were seen in treated birds. Conclusion: The present study introduced P. roseum EO as a potent natural antitrichomonal agent effective against T. gallinae. Bioactive components of P. roseum can be used as potential therapeutic compounds in development of novel antitrichomonal drugs.
https://ajp.mums.ac.ir/article_9605_633dd44be3809dfae12b471dfa5c885e.pdf
2018-03-01
136
142
10.22038/ajp.2017.20763.1788
Pelargonium roseum
Avian trichomoniasis
Metronidazole
Trichomonas gallinae
Pigeon
Mohaddeseh
Abouhosseini Tabari
m_abuhoseini@yahoo.com
1
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Mohammad reza
Youssefi
youssefi2010@gmail.com
2
Department of Veterinary Parasitology, Babol Branch, Islamic Azad University, Babol, Iran
LEAD_AUTHOR
Boal CW, Mannan RW, Hudelson KS. 1998. Trichomoniasis in Cooper's hawks from Arizona. J Wild Life Dis 34: 590-593.
1
Carmen G, Hancu G.2014. Antimicrobial and antifungal activity of Pelargonium roseum essential oils. Adv Pharm Bull, 4: 511.
2
Fakhrie-Kashan Z, Arbabi M, Delavari M, Taghi-Zadeh M, Hooshyar H, Solaymani F. 2014. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. KAUMS Journal 18: 369-375.
3
George D, Biron J, Jolly G Duvallet G, Sparagano O. 2009. Toxicity of geraniol solution in vitro to the poultry red mite, Dermanyssus gallinae. Parasite, 16: 319-321.
4
Gerhold RW, Yabsley MJ, Smith AJ, Ostergaard E, Mannan W, Cann JD, Fischer JR. 2008. Molecular characterization of the Trichomonas gallinae morphologic complex in the United States. J Parasitol 94: 1335-41.
5
Khallaayoune K, Biron J, Chaoui A, Duvallet G. 2009. Efficacy of 1% geraniol (Fulltec®) as a tick repellent. Parasite 16: 223-226.
6
Lis-Balchin M, Hart S, Deans S, Eaglesham E. 1996. Potential agrochemical and medicinal usage of essential oils of Pelargonium species. J.Herbs. Spices. Med. Plants. 3: 11-22.
7
Lumeij JT, Zwijnenberg RJ. 1990. Failure of nitro-imidazole drugs to control trichomoniasis in the racing pigeon (Columba livia domestica). Avian Pathol 19: 165-6.
8
Munoz E, Castella J, Gutierrez JF. 1998. In vivo and in vitro sensitivity of Trichomonas gallinae to some nitroimidazole drugs. Vet. Parasitol. 78: 239-46.
9
Rezai A, Mohajeri D, Pashazadeh M. 2008. Study of histometric and histopathological effects of essential oil of pelargonium roseum in comparison with phenytoin after surgical trauma on rat's skin. Pharmaceut. Sci. 3: 11-19.
10
Rouffaer LO, Adriaensen C, De Boeck C, Claerebout E, Martel A. 2014. Racing pigeons: a reservoir for nitro-imidazole-resistant Trichomonas gallinae. J. Parasitol. 100: 360-3.
11
Sansano-Maestre J, Garijo-Toledo MM, Gómez-Muñoz MT. 2009. Prevalence and genotyping of Trichomonas gallinae in pigeons and birds of prey. Avian. Pathol. 38: 201-207.
12
Seddiek A, El-Shorbagy MM, Khater HF, Ali AM. 2014. The antitrichomonal efficacy of garlic and metronidazole against Trichomonas gallinae infecting domestic pigeons. Parasitol. Res. 113:1319-29.
13
Stockdale JE, Dunn JC, Goodman SJ, Morris AJ, Sheehan DK, Grice PV, Hamer KC. 2015. The protozoan parasite Trichomonas gallinae causes adult and nestling mortality in a declining population of European Turtle Doves, Streptopelia turtur. Parasitology. 142: 490-8.
14
Tabari MA, Youssefi MR, Esfandiari A, Benelli G. 2017a. Toxicity of β-citronellol, geraniol and linalool from Pelargonium roseum essential oil against the West Nile and filariasis vector Culex pipiens (Diptera: Culicidae). Res Vet Sci. 114:36-40.
15
Tabari MA, Youssefi MR, Moghadamnia AA. 2017b. Antitrichomonal activity of Peganum harmala alkaloid extract against trichomoniasis in pigeon (Columba livia domestica). Br poult sci. 12:1-6.
16
Youssefi MR, Tabari MA, Moghadamnia AA. 2017. In vitro and in vivo activity of Artemisia sieberi against Trichomonas gallinae. Iran J Veter Rese, 18:25.
17
ORIGINAL_ARTICLE
Protective effects of long-term administration of Ziziphus jujuba fruit extract on cardiovascular responses in L-NAME hypertensive rats
Objective: Ziziphus jujuba stimulates the release of nitric oxide (NO). Because NO is involved in cardiovascular regulations, in this study the effects of hydroalcoholic extract of Z. jujuba on cardiovascular responses in acute NG-nitro-L-arginine methyl ester (L-NAME) hypertensive rats were evaluated. Materials and Methods: Rats were divided into 6 group (n=6): 1) saline, 2) L-NAME received (10mg/kg) intravenously, 3) sodium nitroprusside (SNP) (50µg/kg)+L-NAME group received SNP before L-NAME and 4-6) three groups of Z. jujuba (100, 200 and 400mg/kg) that treated for four weeks and on the 28th day, L-NAME was injected. Femoral artery and vein were cannulated for recording cardiovascular responses and drug injection, respectively. Systolic blood pressure (SBP), Mean arterial pressure (MAP) and heart rate (HR) were recorded continuously. Maximal changes (∆) of SBP, MAP and HR were calculated and compared to control and L-NAME groups. Results: In L-NAME group, maximal ΔSBP (L-NAME: 44.15±4.0 mmHg vs control: 0.71±2.1 mmHg) and ΔMAP (L-NAME: 40.8±4.0 mmHg vs control: 0.57±1.6 mmHg) significantly increased (p0.05). All doses of Z. jujuba attenuated maximal ∆SBP and ∆MAP induced by L-NAME but only the lowest dose (100 mg/kg) had significant effects (ΔSBP: 20.36±5.6 mmHg vs L-NAME: 44.1±4.0 mmHg and ΔMAP: 20.8±4.5 mmHg vs L-NAME: 40.8±3.8 mmHg (p0.05). Conclusion: Because long-term consumption of Z. jujuba extract, especially its lowest dose, attenuated cardiovascular responses induced by L-NAME, we suggest that Z. jujuba has potential beneficial effects in prevention of hypertension induced by NO deficiency.
https://ajp.mums.ac.ir/article_9920_112f770928c8f220ad526b4180bbcd81.pdf
2018-03-01
143
151
10.22038/ajp.2017.24232.1887
Ziziphus jujuba
Nitro-L-arginine methyl ester
blood pressure
Hypertension
Nitric oxide
Reza
Mohebbati
mohebbatir931@mums.ac.ir
1
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Kosar
Bavarsad
bavarsadk911@mums.ac.ir
2
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Maryam
Rahimi
rahimim921@mums.ac.ir
3
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Hasan
Rakhshandeh
rakhshandehh@mums.ac.ir
4
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Abolfazl
Khajavirad
khajavirada@mums.ac.ir
5
Neurogenic Inflammation Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mohammad Naser
Shafei
shafeimn@mums.ac.ir
6
Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Abbasnezhad A, Khazdair MR, Kianmehr M. 2016. The role of nitric oxide on the oxytocin induce analgesia in mice. Iran J Basic Med Sci, 19: 238-244.
1
Achike FI, Kwan CY. 2003. Nitric oxide, human diseases and the herbal products that affect the nitric oxide signalling pathway. Clin Exp Pharmacol Physiol, 30:605-615.
2
Al-Reza SM, Yoon JI, Kim HJ, Kim JS, Kang SC .2010. Anti-inflammatory activity of seed essential oil from Zizyphus jujuba. Food Chem Toxicol, 48:639-643.
3
Banting JD, Friberg P, Adams MA. 1996. acute hypertension after nitric oxide synthase inhibition is mediated primarily by increased endothelin vasoconstriction. J Hypertens, 14:975-82.
4
Biancardi VC, Bergamaschi CT, Lopes OU, Campos RR. 2007. Sympathetic activation in rats with L-NAME-induced hypertension. Braz J Med Biol Res, 40:401-8.
5
Cheng G, Bai Y, Zhao Y, Tao J, Liu Y, Tu G, Ma L, LiaO N, XU X. 2000. Flavonoids from Ziziphus jujuba Mill var. spinosa. Tetrahedron, 56:8915-8920.
6
Fadel PJ. 2017. Nitric Oxide and Cardiovascular Regulation. Am Heart Assoc, 2017:778-779.
7
Fisher NDL, Hughes M, Gerhard-Herman M, Hollenberg NK. 2003. Flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in healthy humans. J Hypertens, 21:2281-2286.
8
Freedman JE, Parker C, Li L, Perlman JA, Frei B, Ivanov V, Deak LR, Iafrati MD, Folts JD. 2001. Select flavonoids and whole juice from purple grapes inhibit platelet function and enhance nitric oxide release. Circulation, 103:2792-2798.
9
Gao Q-H, Wu C-S, Wang M. 2013. the jujube (Ziziphus jujuba Mill.) fruit: a review of current knowledge of fruit composition and health benefits. J Agric Food Chem, 61:3351-3363.
10
Goyal R, Sharma PL, Singh M. 2011. Possible attenuation of nitric oxide expression in anti-inflammatory effect of Ziziphus jujuba in rat. J Nat Med, 65:514-518.
11
Han YJ, Kwon YG, Chung HT, Lee SK, Simmons RL, Billiar TR, Kim YM. 2001. Antioxidant enzymes suppress nitric oxide production through the inhibition of NF-κB activation: role of H2O2 and nitric oxide in inducible nitric oxide synthase expression in macrophages. Nitric Oxide, 5:504-513.
12
Hirschl MM, Binder M, Bur A, Herkner H, Müllner M, Woisetschläger C, Laggner AN. 1997. Safety and efficacy of urapidil and sodium nitroprusside in the treatment of hypertensive emergencies. Intensive Care Med, 23:885-888.
13
Hu CT, Chang KC, Wu CY, Chen HI. 1997. Acute effects of nitric oxide blockade with L‐NAME on arterial haemodynamics in the rat. Br J Pharmacol, 122:1237-1243.
14
Huang X, Kojima-Yuasa A, Norikura T, Kennedy DO, Hasuma T, Matsui-Yuasa I. 2007. Mechanism of the anti-cancer activity of Zizyphus jujuba in HepG2 cells. Am J Chin Med, 35:517-532.
15
Jerkic M, Rivas-Elena JV, Prieto M, et al. 2004. Endoglin regulates nitric oxide-dependent vasodilatation. FASEB J, 18:609-611.
16
Khayyal MT, El-Ghazaly MA, Abdallah DM, Nassar NN, Okpanyi SN, Kreuter M-H, 2002. Blood Pressure Lowering Effect of an Olive Leaf Extract {Olea europaed) in L-NAME Induced Hypertension in Rats. Arzneimittelforschung, 52:797-802.
17
Kim H, Han S. 1996. Zizyphus jujuba and Codonopsis pilosula stimulate nitric oxide release in cultured endothelial cells and kidney tissues. Asia Pac J Pharmacol, 11:121-128.
18
Klimaschewski L, Kummer W, Mayer B, et al. 1992. Nitric oxide synthase in cardiac nerve fibers and neurons of rat and guinea pig heart. Circ Res, 71:1533-1537.
19
Mahajan RT, Chopda M. 2009. Phyto-Pharmacology of Ziziphus jujuba Mill-A plant review. Pharmacogn Rev, 3:320 -329.
20
Mohebbati R, Iranmanesh M, Beheshti F, et al. 2016. The Effect of Some Herbal Extracts on Nitric Oxide Production in Endothelial Cells 3T3 Cell Line. Iran J Pharm Sinc, 12:1-10.
21
Mohebbati R, Shafei MN, Soukhtanloo M, Roshan NM, Rad AK, Anaeigoudari A, Hosseinian S, Karimi S, Beheshti F. 2016. Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney. Avicenna J Phytomed, 6:86-94.
22
Morley JE, Farr SA, Sell RL, Hileman SM, Banks WA. 2011. Nitric oxide is a central component in neuropeptide regulation of appetite. Peptides, 32:776-780.
23
Naseem KM. 2005. The role of nitric oxide in cardiovascular diseases. Mol Aspects Med, 26:33-65.
24
Sasser JM, Molnar M, Baylis C. 2011. Relaxin ameliorates hypertension and increases nitric oxide metabolite excretion in angiotensin II but not Nω-nitro-L-arginine methyl ester hypertensive rats. Hypertension, 58:197-204.
25
Savoia C, Schiffrin EL. 2006. Inflammation in hypertension. Curr Opin Nephrol Hypertens, 15:152-158.
26
Schulz E, Gori T, Münzel T. 2011. Oxidative stress and endothelial dysfunction in hypertension. Hypertens Res, 34:665-673.
27
Shafei MN, Nasimi A. 2011. Effect of glutamate stimulation of the cuneiform nucleus on cardiovascular regulation in anesthetized rats: Role of the pontine Kolliker–Fuse nucleus. Brain Res, 1385:135-143.
28
Steinkamp-Fenske K, Bollinger L, Xu H, Yao Y, Horke S, Förstermann U, Li H. 2007. Reciprocal regulation of endothelial nitric-oxide synthase and NADPH oxidase by betulinic acid in human endothelial cells. J Pharmacol Exp Ther, 322:836-842.
29
Stewart DE. 2004. Venlafaxine and sour date nut. A J Psychiatry, 161:1129.
30
Taati M, Alirezaei M, Meshkatalsadat M, Rasoulian B, Kheradmand A, Neamati S. 2011. Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the rat testes. Iran J Vet Res, 12:39-45.
31
Wang D, Zhao Y, Jiao Y, Yu L, Yang S, Yang X. 2012. Antioxidative and hepatoprotective effects of the polysaccharides from Zizyphus jujube cv. Shaanbeitanzao. Carbohydr Polym, 88:1453-1459.
32
Xiaoling H, Zhiyun X, Yi N, Jinliang L, Xianqiong F. 1992. Outline of the Investigation on the leaf of F. tataricum bythe Means of Traditional Chinese Medicine and Western Modem Medicine. 470-476.
33
Zhang D, Yuan B, Sun H. 2002. the effect of jujuboside on rats with spontaneous hypertension. J Xi'an Jiaotong Univers, 24:59-60.
34
Zhang H, Jiang L, Ye S, Ye Y, Ren F. 2010. Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube (Ziziphus jujuba Mill.) from China. Food Chem Toxicol, 48:1461-1465.
35
Zhao Y, Zhang X, Li J, Bian Y, Sheng M, Liu B, Fu Z, Zhang Y, Yang B. 2016. Jujuboside B Reduces Vascular Tension by Increasing Ca 2+ Influx and Activating Endothelial Nitric Oxide Synthase. PloS one, 11:149386.
36
Zicha J, Dobešová Z, Kuneš J. 2006. Antihypertensive mechanisms of chronic captopril or N-acetylcysteine treatment in L-NAME hypertensive rats. Hypertens Res, 29:1021-1027.
37
ORIGINAL_ARTICLE
Effects of IMOD™ on angiogenesis, miR-503 and CDC25 expression levels in heart tissue of diabetic male rats
Objective: Diabetes is associated with vascular complications and impaired angiogenesis. Since angiogenesis plays a crucial role in vascular homeostasis in ischemic heart diseases, in this study, the effect of IMOD™ on miR-503 and CDC25 expression level which are altered in impaired angiogenesis were investigated in heart tissue of diabetic rats. Materials and Methods: Forty male Wistar rats (200-250 g) were randomly classified into 4 groups: control (C), IMOD™ (I), diabetes (D), and diabetes+IMOD™ (D+I). For induction of experimental diabetes in animals, a single dose of streptozotocin (STZ; 60mg/kg) was injected intraperitoneally. After 8 weeks of treatment with IMOD™ (20 mg/kg/day), heart tissue samples were removed and used for measurement of miR-503 and CDC25 expression level as well as histological studies. Results: Results of this study showed that diabetes decreased heart tissue angiogenesis which was associated with increased miR-503 and reduced CDC25 expression levels (p<0.05) and IMOD™ could reduce the expression of miR-503 and increase the expression of CDC25 (p<0.05). Moreover, IMOD™ extensively induced angiogenesis in the heart tissue of diabetic group. However, IMOD™ had no significant effect on expressions of miR-503 and CDC25, or angiogenesis in healthy rats. Conclusion: This study showed that IMOD™ is able to increase angiogenesis in the heart tissue of diabetic rats. The angiogenic effect of IMOD™ is associated with reduction of miR-503 expression and increased expression of CDC25.
https://ajp.mums.ac.ir/article_9682_8369912f1f26b0033094a7442ff8ef61.pdf
2018-03-01
152
160
10.22038/ajp.2017.23107.1856
Diabetes
Angiogenesis
miR-503
CDC25
IMOD
Arshad
Ghaffari-Nasab
ghaffari_arshad@yahoo.com
1
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
AUTHOR
Fariba
Mirzaei Bavil
bavil2000@yahoo.com
2
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
AUTHOR
Rafigheh
Ghiasi
faghiasi2@gmail.com
3
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
AUTHOR
Saeed
Sadigh-Eteghad
saeed.sadigetegad@gmail.com
4
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
AUTHOR
Mohammad Reza
Alipour
alipourmr52@gmail.com
5
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
LEAD_AUTHOR
Abacı A, Oğuzhan A, Kahraman S, Eryol NK, Ünal Ş, Arınç H, Ergin A. 1999. Effect of Diabetes Mellitus on Formation of Coronary Collateral Vessels. Circulation, 99: 2239-2242.
1
Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi S, Farhangi A, Allah Verdi A, Mofidian SMA, Lame Rad B. 2013. Induction of diabetes by streptozotocin in rats. Indian J Clin Biochem, 22: 60-64.
2
Alipour MR, Khamaneh AM, Yousefzadeh N, Mohammad-nejad D, GhadiriSoufi F. 2013. Upregulation of microRNA-146a was not accompanied by downregulation of pro-inflammatory markers in diabetic kidney. Mol Biol Rep, 40: 6477–6483.
3
Alva ML, Gray A, Mihaylova B, Leal J, Holman RR. 2015. The impact of diabetes‐related complications on healthcare costs: new results from the UKPDS. Diabetic Med, 32: 459-466.
4
Bajpai S, Mishra M, Kumar H, Tripathi K, Singh SK, Pandey HP, Singh RK. 2011. Effect of selenium on connexin expression, angiogenesis, and antioxidant status in diabetic wound healing. Biol Trace Elem Res, 144: 327-338.
5
Caporali, A, Meloni M, Völlenkle C, Bonci D, Sala-Newby GB, Addis R, Spinetti G, Losa S, Masson R, Baker AH, Agami R, Sage CL, Condorelli G, Madeddu P, Martelli F, Emanueli C. 2011. Deregulation of microRNA-503 Contributes to Diabetes Mellitus–Induced Impairment of Endothelial Function and Reparative Angiogenesis After Limb Ischemia. Circulation, 123: 282-291.
6
Carmeliet P. 2005. Angiogenesis in life, disease and medicine. Nature, 438: 932-936
7
Chang TC, Mendell JT. 2007. microRNAs in Vertebrate Physiology and Human Disease. Annu Rev Genomics Hum Genet, 8: 215-239.
8
Deepthi B, Sowjanya K, Lidiya B, Bhargavi RS, Babu PS. 2017. A Modern Review of Diabetes Mellitus: An Annihilatory Metabolic Disorder. J In Silico In Vitro Pharmacol, 3:1 .
9
Donzelli M, Draetta GF. 2003. Regulating mammalian checkpoints through Cdc25 inactivation. EMBO reports, 4: 641-728.
10
Farhoudi M, Najafi-Nesheli M, Hashemilar M, Mahmoodpoor A, Sharifipour E, Baradaran B, Taheraghdam A, Savadi-Oskouei D, Sadeghi-Bazargani H, Sadeghi-hokmabadi E, Akbari H, Rikhtegar R. 2013. Effect of IMOD™ on the inflammatory process after acute ischemic stroke: a randomized clinical trial. DARU J Pharm Sci, 21: 26.
11
Forbes JM, Cooper ME. 2013. Mechanisms of diabetic complications. Physiol Rev, 93: 137-188.
12
Furman BL. 2015. Streptozotocin‐induced diabetic models in mice and rats. Curr Protoc Pharmacol, 47: 1-20.
13
Heather LC, Clarke K. 2011. Metabolism, hypoxia and the diabetic heart J Mol Cell Cardiol, 50: 598-605.
14
Hormozi M, Talebi S, Khorram Khorshid HR, Zarnani AH, Kamali K, Jeddi-Tehrani M, Soltangoraee H, Akhondi MM. 2015. The effect of Setarud (IMODTM) on angiogenesis in transplanted human ovarian tissue to nude mice. Iran J Reproduct Med, 13: 605-614.
15
Huynh K, Bernardo BC, McMullen JR, Ritchie RH. 2014. Diabetic cardiomyopathy: mechanisms and new treatment strategies targeting antioxidant signaling pathways. Pharmacol therapeut, 142: 375-415.
16
Ikeoka K, Nakaoka Y, Arita Y, Hashimoto T, Yasui T, Masaki T, Yamauchi-Takihara K, Shirai M, Komuro I, Sakata Y. 2014. Angiopoietin-1 Derived From Vascular Smooth Muscle Cells is Essential for Arteriogenesis After Hindlimb Ischemia. Circulation, 130: A15664-A15664.
17
Khazaei M, Salehi E. 2013. Myocardial capillary density in normal and diabetic male rats: Effect of bezafibrate. Res Pharm Sci, 8: 119-123.
18
Kolluru GK, Bir SC, Kevil CG. 2012. Endothelial dysfunction and diabetes: effects on angiogenesis, vascular remodeling, and wound healing. Int J Vascul Med, Article ID 918267.
19
Kong W, Zhao J, He L, Cheng JQ. 2009. Strategies for Profiling MicroRNA Expression. J Cell Physiol, 218: 22-25.
20
Kota SK, Meher LK, Jammula S, Kota SK, Krishna S, Modi KD. 2012. Aberrant angiogenesis: The gateway to diabetic complications. India J Endocrinol Metabol, 16: 918.
21
Leeper NJ, Cooke JP. 2011. MicroRNA and mechanisms of impaired angiogenesis in diabetes mellitus. Circulation, 123: 236-238.
22
Liu H, Yu S, Zhang H, Xu J. 2012. Angiogenesis Impairment in Diabetes: Role of Methylglyoxal-Induced Receptor for Advanced Glycation Endproducts, Autophagy and Vascular Endothelial Growth Factor Receptor 2. PLOS ONE, 7: e46720.
23
MirzaeiBavil F, Alipour MR, Keyhanmanesh R, Alihemmati A, Ghiyasi R, Mohaddes G. 2015. Ghrelin Decreases Angiogenesis, HIF-1α and VEGF Protein Levels in Chronic Hypoxia in Lung Tissue of Male Rats. Adv Pharm Bull, 5: 315-320.
24
Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA. 2007. Indian herbs and herbal drugs for the treatment of diabetes. J Clin Biochem Nutr, 40: 163-173.
25
Mohammadirad A, Khorram-Khorshid HR, Gharibdoost F, Abdollahi M. 2011. Setarud (IMODTM) as a Multiherbal Drug with Promising Benefits in Animal and Human Studies: A Comprehensive Review of Biochemical and Cellular Evidences. J Animal Veter Advances, 6: 1185-1192.
26
Mohseni-Salehi-Monfared SS, Habibollahzadeh E, Sadeghi H, Baeeri M, Abdollahi M. 2010. Efficacy of Setarud (IMOD™), a novel electromagnetically-treated multi-herbal compound, in mouse immunogenic type-1 diabetes. Arch Med Sci, 6: 663-669.
27
Paydary K, Emamzadeh-Fard S, Khorram Khorshid HR, Kamali K, SeyedAlinaghi SA, Mohraz M. 2012. Safety and efficacy of Setarud (IMOD TM) among people living with HIV/AIDS: a review. Recent Patents on Anti-Infective Drug Discovery, 7: 66-72.
28
Rippe C, Blimline M, Magerko KA, Lawson BR, LaRocca TJ, Donato AJ, Seals D R. 2012. MicroRNA changes in human arterial endothelial cells with senescence: relation to apoptosis, eNOS and inflammation. Exp gerontol, 47: 45-51.
29
Sarkar S, Dey BK, Dutta A. 2010. MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A. Mol Biol Cell, 21: 2138-2149.
30
Shibuya M. 2011. Vascular Endothelial Growth Factor (VEGF) and Its Receptor (VEGFR) Signaling in Angiogenesis: A Crucial Target for Anti- and Pro-Angiogenic Therapies. Genes Cancer, 2: 1097-1105.
31
Tabatabaei-Malazy O, Larijani B, Abdollahi M. 2013. A novel management of diabetes by means of strong antioxidants’ combination. J Med Hypothes Ideas, 7: 25-30.
32
Tahergorabi Z, Khazaei M. 2012. Imbalance of Angiogenesis in Diabetic Complications: The Mechanisms. Int J Prevent Med, 3: 827-838.
33
Timofeev O, Cizmecioglu O, Settele F, Kempf T, Hoffmann I. 2012. Cdc25 Phosphatases Are Required for Timely Assembly of CDK1-Cyclin B at the G2/M Transition. J Biol Chem, 285: 16978-16990.
34
Xu L, Kanasaki K, Kitada M, Koya D. 2012. Diabetic angiopathy and angiogenic defects. Fibrogenesis tissue repair, 5: 1
35
Yamakuchi M. 2012. MicroRNAs in Vascular Biology. Int J Vasc Med, Article ID 794898.
36
ORIGINAL_ARTICLE
A comparison of the effects of Portulaca oleracea seeds hydro-alcoholic extract and Vitamin C on biochemical, hemodynamic and functional parameters in cardiac tissue of rats with subclinical hyperthyroidism
Objective: The present study was performed to evaluate the effects of hydro-alcoholic extract of Portulaca oleracea (P. oleracea) seeds and Vitamin C on biochemical and hemodynamic parameters in cardiac tissue of rats with subclinical hyperthyroidism. Materials and Methods: Forty eight male rats were divided into six groups of 8 and treated for 4 weeks. T4 group received daily injection of levothyroxine sodium (20 μg/kg) and control group was given daily injection of saline. T4-Po groups were given T4 plus 100, 200, and 400 mg/kg of P. oleracea seeds extract in drinking water daily. T4-Vit C group received T4 plus daily injection of Vitamin C (100 mg/kg). At the end of the experiment, body weight, serum free T4 level, left ventricular developed pressure (LVDP), malondialdehyde (MDA) and total thiol levels were measured. Results: Free T4 levels were increased in all groups that were treated with T4. Weight gain was decreased in T4 and T4-Po100 groups compared to control group (p Conclusion: The results showed that P. oleracea extract has a protective effect on cardiac dysfunction due to subclinical hyperthyroidism induced by levothyroxine sodium in rats.
https://ajp.mums.ac.ir/article_9965_492b546fa2352e5bea0f9851c4699e87.pdf
2018-03-01
161
169
10.22038/ajp.2017.21556.1812
Portulaca oleracea seeds
Vitamin C
Heart
Subclinical hyperthyroidism
Rat
Hadi
Khodadadi
khodadadih921@mums.ac.ir
1
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Roghayeh
Pakdel
pakdelr901@mums.ac.ir
2
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Majid
Khazaei
khazaeim@mums.ac.ir
3
Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Saeed
Niazmand
niazmands@mums.ac.ir
4
Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Kowsar
Bavarsad
bavarsadk911@mums.ac.ir
5
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mousa-al-reza
Hajzadeh
hajzadehmr@mums.ac.ir
6
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Aghili MH. 1992. Makhzan-al-Advia, pp: 227-228, Tehran.
1
Bielecka-Dabrowa A, Mikhailidis DP, Rysz J, Banach M. 2009. The mechanisms of atrial fibrillation in hyperthyroidism. Thyroid Res, 2: 1.
2
Biondi B, Palmieri EA, Lombardi G, Fazio S. 2002. Effects of subclinical thyroid dysfunction on the heart. Ann Intern Med, 137: 904-914.
3
Boskabady MH, Hashemzehi M, Khazdair MR, Askari VR. 2016. Hydro-ethanolic Extract of Portulaca oleracea Affects Beta-adrenoceptors of Guinea Pig Tracheal Smooth Muscle. Iran J Pharm Res, 15: 867.
4
Chan K, Islam MW, Kamil M, Radhakrishnan R, Zakaria MN, Habibullah M, Attas A. 2000. The analgesic and anti-inflammatory effects of
5
Portulaca oleracea L. subsp. sativa (Haw.) Celak. J Ethnopharmacol, 73: 445-451.
6
Chen Y-F, Weltman NY, Li X, Youmans S, Krause D, Gerdes AM. 2013. Improvement of left ventricular remodeling after myocardial infarction with eight weeks L-thyroxine treatment in rats. J Transl Med, 11: 40.
7
Dos Reis-Lunardelli EA, Castro CC, Bavaresco C, 2007. Effects of thyroid hormones on memory and on Na+, K+-ATPase activity in rat brain. Curr Neurovasc Res, 4: 184-193.
8
Dweck AC. 2013. Purslane (P. oleracea)-the global panacea. Perso Care Mag, 2: 7-15.
9
Ellman GL. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys, 82: 70-77.
10
Fazio S, Palmieri EA, Lombardi G, Biondi B. 2004. Effects of thyroid hormone on the cardiovascular system. Recent Prog Horm Res, 59: 31-50.
11
Gao C, Li T, Liu J, Guo Q, Tian L. 2015. Endothelial functioning and hemodynamic parameters in rats with subclinical hypothyroid and the effects of thyroxine replacement. PloS one, 10:e0131776.
12
Gredilla R, Barja G, López-Torres M. 2001. Thyroid hormone-induced oxidative damage on lipids, glutathione and DNA in the mouse heart. Free Radic Res, 35: 417-425.
13
Hajzadeh M, Rakhshandeh H, Esmaeilizadeh M, Ghorbani A. 2004. Analgesic and anti-inflammatory effects of Portolaca oleracea extracts in mice & rat. Faslnamahi Kumish, 5: 113-120.
14
Hashemzehi M, Khazdair M, Kiyanmehr M, Askari V, Boskabady M. 2016. Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle. Indian J Pharm Sci, 78: 388-394.
15
Jacob RA, Sotoudeh G. 2002. Vitamin C function and status in chronic disease. Nutr Clin Care, 5: 66-74.
16
Janero DR. 1990. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9: 515-540.
17
Karimi G, Khoei A, Omidi A, 2010. Protective effect of aqueous and ethanolic extracts of Portulaca oleracea against cisplatin induced nephrotoxicity. Iran J Basic Med Sci, 13: 31-35.
18
Karmisholt J, Andersen S, Laurberg P. 2011. Weight loss after therapy of hypothyroidism is mainly caused by excretion of excess body water associated with myxoedema. J Clin Endocrinol Metab, 96: E99-E103.
19
Kaveh M, Eidi A, Neamati A, Boskabady MH. 2017. Modulation of lung inflammation and immune markers in asthmatic rats treated by Portulaca oleracea. Avicenna J Phytomed: 1-11.
20
Li X, Long Y, Fang X, Liu X. 2008. Effect of vitamin C and E on antioxidative enzyme, NOS activity and NO contents in hippocampus of rats with lead poisoning. Zhejiang Da Xue Xue Bao Yi Xue Ban, 37: 189-192.
21
Liao H-F, Chen Y-Y, Liu J-J, 2003. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem, 51: 7907-7912.
22
Lim Y, Quah E. 2007. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem, 103: 734-740.
23
Mamoun Ma, Rizvi Hsmta, Qazi Sm. 2015. Protective Effect of Vitamin C on Body Weight of Albino Rats with Lead Toxicity. Pak J Med Health Sci, 9
24
Marriott ML, McNeill JH. 1983. Effect of thyroid hormone treatment on responses of the isolated working rat heart. Can J Physiol Pharmacol, 61: 1382-1390.
25
Okafor IA, Ezejindu DN. 2014. Phytochemical studies on Portulaca oleracea (purslane) plant. GJBAHS, 3: 132-136.
26
Okwuasaba F, Ejike C, Parry O. 1987. Effects of extracts of Portulaca oleracea on skeletal muscle in vitro. J Ethnopharmacol, 21: 55-63.
27
Parry O, Okwuasaba F, Ejike C. 1988. Effect of an aqueous extract of Portulaca oleracea leaves on smooth muscle and rat blood pressure. J Ethnopharmacol, 22: 33-44.
28
Pijl H, De Meijer P, Langius J, 2001. Food choice in hyperthyroidism: potential influence of the autonomic nervous system and brain serotonin precursor availability. J Clin Endocrinol Metab, 86: 5848-5853.
29
Sultana A, Rahman K. 2013. Portulaca oleracea Linn. A global Panacea with ethno-medicinal and pharmacological potential. Int J Pharm Pharm Sci, 5: 33-39.
30
Sun H, He X, Liu C, 2016. Effect of Oleracein E, a Neuroprotective Tetrahydroisoquinoline, on Rotenone-Induced Parkinson’s Disease Cell and Animal Models. ACS Chem Neurosci. 8: 155-164.
31
Toft AD. 2001. Subclinical hyperthyroidism. N Engl J Med, 345: 512-516.
32
Venditti P, Balestrieri M, Di Meo S, De Leo T. 1997. Effect of thyroid state on lipid peroxidation, antioxidant defences, and susceptibility to oxidative stress in rat tissues. J Endocrinol, 155: 151-157.
33
Venditti P, Di Meo S. 2006. Thyroid hormone-induced oxidative stress. Cell Mol Life Sci, 63: 414-434.
34
Wu C-y, Liu B, Wang H-l, Ruan D-y. 2011. Levothyroxine rescues the lead-induced hypothyroidism and impairment of long-term potentiation in hippocampal CA1 region of the developmental rats. Toxicol Appl Pharmacol, 256: 191-197.
35
Yang Z, Liu C, Xiang L, Zheng Y. 2009. Phenolic alkaloids as a new class of antioxidants in Portulaca oleracea. Phytother Res, 23: 1032-1035.
36
Yazici I, Türkan I, Sekmen AH, Demiral T. 2007. Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environ Exp Bot, 61: 49-57.
37
Yen GC, Chen HY, Peng HH. 2001. Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants. Food Chem Toxicol, 39: 1045-1053.
38
Yu D, Zhou H, Yang Y, 2015. The bidirectional effects of hypothyroidism and hyperthyroidism on anxiety-and depression-like behaviors in rats. Horm Behav, 69: 106-115.
39
Zhang PY, Xu X, Li XC. 2014. Cardiovascular diseases: oxidative damage and antioxidant protection. Eur Rev Med Pharmacol Sci, 18: 3091-3096.
40
ORIGINAL_ARTICLE
Preventive effects of the aqueous extract of Cichorium intybus L. flower on ethylene glycol-induced renal calculi in rats
Objective: Urolithiasis remains a global problem. Despite the availability of numerous methods, no definite therapeutic agent has been yet introduced for the prevention or treatment of kidney stones. In this study, we evaluated the possible preventive effects of aqueous extract of Cichorium intybus L. (chicory) flowers on ethylene glycol-induced renal calculi in rats. Materials and Methods: A total of 24 Wistar rats were randomly divided into four groups and were treated for 30 days. Group A received drinking tap water, while groups B, C, and D were administered with 1% ethylene glycol for induction of calcium oxalate stone formation. Rats in groups C and D received intraperitoneal injections of the aqueous extract of chicory flowers (50 and 200 mg/kg, respectively) since the first day of the experiment. The urine volume, urine pH, and urinary levels of oxalate, citrate, calcium, uric acid, and creatinine as well as serum levels of calcium, uric acid, and creatinine were measured. After 30 days, the rats' kidneys were removed and prepared for histological evaluation of calcium oxalate deposits. One-way analysis of variance (ANOVA), followed by Tukey's test, was performed, using SPSS version 20. Results: The number of calcium oxalate crystals was significantly higher in group B (ethylene glycol-only treated animals), compared to group A (control), group C (50 mg/kg of aqueous extract), and group D (200 mg/kg of aqueous extract) (p<0.05). On day 30, the urine level of citrate, oxalate (p>0.05), and creatinine (p<0.05), as well as urine pH (p<0.05) decreased in groups C and D, compared to group B. Also, urine calcium level, urine uric acid (p>0.05), and urine volume (p<0.05) were higher in group D, compared to group B. In addition, the serum level of calcium, creatinine (p<0.05), and uric acid (p<0.001) decreased in groups C and D. Conclusion: The aqueous extract of chicory flower (50 mg/kg) could reduce the number of calcium oxalate deposits in the urine and reduce the level of serum parameters.
https://ajp.mums.ac.ir/article_10001_a005539ffc6c381d69db7ae1a7cc1fbb.pdf
2018-03-01
170
178
10.22038/ajp.2017.21952.1824
Cichorium intybus L
Flower
Renal calculi
Ethylene glycol
Mahdieh
Emamiyan
mahdie.zaman@yahoo.com
1
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
AUTHOR
Gholamhassan
Vaezi
mahdie.imamian@gmail.com
2
Department of Biology, Faculty of Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
LEAD_AUTHOR
Maryam
Tehranipour
maryamdayani1@yahoo.com
3
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
AUTHOR
Khdije
Shahrohkabadi
shahrokhabady@yahoo.com
4
Molecular Genetic Ph.D, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
AUTHOR
Abdolhossein
Shiravi
shiravi738@yahoo.com
5
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
AUTHOR
Al-Snafi AE. 2016. Medical importance of Cichorium intybus – A review. IOSR J Pharmacy, 6: 41-56.
1
Aslan Z, Aksoy L. 2015. Anti-inflammatory effects of royal jelly on ethylene glycolinduced renal inflammation in rats.Res. J Pharm Sci, 2: 1-4.
2
De Kraker J-W, Franssen MCR, Dalm MCF, de Groot A, Bouwmeester HJ. 2001. Biosynthesis of germacrene a carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase (s) involved in sesquiterpene lactone biosynthesis. J Plant Physiol, 125: 1930-1940.
3
El- Dakhakhny M, Madi N. J, Ammon H. P. 2002. Nigella sativa oil, nigellone and derivedthymoquinone inhibit synthesis of 5-Lipoxygenase products in polymorphonuclear leukocytes from rats. J ethnopharmacol, 81: 161-164.
4
Eslami H. 2015. Investigation the effects of used solvent components proportions for extraction the antimicrobial compounds of Cichorium intybus L. on their antibacterial and antifungal activities. Int J Biosci , 6: 73-81.
5
Fatemi Tabatabaei SR, Rashno M, Ghaderi Sh, Askaripour M. 2016. The Aqueous Extract of Portulaca Oleracea Ameliorates Neurobehavioral Dysfunction and Hyperglycemia Related to Streptozotocin-Diabetes Induced in Ovariectomized Rats. Iran J Pharm Res, 15: 561-571.
6
Gupta M, Bhayana S, Sikka SK. 2011. Role of urinary inhibitors and promoters in calcium oxalate crystallisation. Int J Research in Pharmacy and Chemistry, 1: 793-798.
7
Hadjzadeh MAR, Khoei R, Hadjzadeh Z, Parizady M. 2007. Ethanolic extract of nigella sativa L seeds on ethylene glycol-induced kidney calculi in rats. J Urol, 4: 86-90.
8
Jamshidzadeh A, Khoshnood MJ, Dehghani Z, Niknahad H. 2006. Hepatoprotective activity of Cichorium intybus L. leaves extract against carbon tetrachloride induced toxicity. Iran J Pharm Res, 1:41-46.
9
Katiyar P, Kumar A, Mishra AK, Dixit RK, Kumar A, Kumar R, Gupta AK. 2015. Kasni (Cichorium intybus L.) A propitious traditional medicinal herb. Int J Pharmacogn, 8:368-380.
10
Khajavi Rad A, Hadjzadeh MA, Rajaei Z, Mohammadian N, Valiollahi S, Sonei M. 2011. The Beneficial effect of Cynodon Dactylon fractions on ethylene glycol-induced kidney calculi in rats. J Urol, 8: 179-184.
11
Khan S. R, Thamilselvan S. 2000, Nephrolithiasis: a consequence of renal epithelial cell exposure to oxalate and calcium oxalate crystals. J Mol Urol, 4: 305-312.
12
Khksari M, Rezvani ME, Salado MA, Soleimani A. 2000. The effect of topically applied water extract of Rhazya stricta on cutaneous wound healing in rats.J Koomesh, 1: 1-10.
13
Mehmood N, Zubair M, Rowen K, Rasool N, Shahid M, Ahmad VU. 2012. Antioxidant, antimicrobial and phytochemical analysis of Cichorium intybus seeds extract and various organic fractions. Iran J Pharm Res, 11: 1145-1151.
14
Mehrabi S, Askarpou E,Mehrabi F, Jannesar R. 2016. Effects of hydrophilic extract of Nasturtium officinale on prevention of ethylene glycol induced renal stone in male Wistar rats. J Nephropathol, 4: 123-127.
15
Nandagopal S, Ranjitha Kumari BD.2007. Phytochemical and Antibacterial Studies of Chicory (Cichorium intybus L.) - A Multipurpose Medicinal Plant. A J Biol Res, 1: 17-21.
16
Pomara C, Fiore C, D'Errico S, Riezzo I, Fineschi V. 2008. A confocal laser scanning microscope study in a fatal case. Clin Toxicol (Phila), 46: 322-324.
17
Rampoldi L, Scolari F, Amoroso A, Ghiggeri G, Devuyst O. 2011. The rediscovery of uromodulin (Tamm–Horsfall protein): from tubulointerstitial nephropathy to chronic kidney disease. Kidney Int, 80: 338-347.
18
R.Hoyer J, W.Seiler M. 1979. Pathophysiology of Tamm-Horsfall protein. Kidney Int, 16: 279-289.
19
Rashed K, Medda R, Spano D, Pintus F. 2016. Evaluation of antioxidant, anti-tyrosinase potentials and phytochemical composition of four egyptian plants. Int Food Res J, 23: 203-210.
20
Shad MA, Nawaz H, Rehman T, Ikram N. 2013. Determination of some biochemicals, phytochemicals and antioxidant properties of different parts of Cichorium intybus L.: A comparative study. J Anim Plant Sci, 23: 1060-1066.
21
Shafaeifar A, Mehrabi S, Malekzadeh J, Jannesar R, Sadeghi H, Vahdani R, Mohammadi R. 2011. Effect of Hydrophilic Extract of Alhagi Maurorum on Ethylene Glycol-Induced Renal Stone in Male Wistar Rats.J Armaghane danesh, 17: 129-138.
22
Shaikh T, Mujum A, Wasimuzzama K, Rub RA. 2010. An overview on phytochemical and pharmacological profile of Cichorium intybus Linn. Br J Pharmacol, 2: 298-307.
23
Sridharan B, Micheal ST, Ramachandran A, Ganesh RN, Viswanathan P. 2015. Citrus bioflavonoids ameliorate hyperoxaluria induced renal injury and calcium oxalate crystal deposition in Wistar rats. Adv Pharm Bull, 5:1-9.
24
Street RA, Sidana J, Prinsloo G. 2013. Cichorium intybus: traditional uses, phytochemistry, pharmacology, and toxicology. Evid Based Complement Alternat Med, 2013:1-13.
25
Zaman R, Noorul Basar S. 2013. A review article of Beekhe Kasni (Cichorium intybus) its traditional uses and pharmacological actions. Int Braz J Urol, 41: 1008-1013.
26
ORIGINAL_ARTICLE
Doxorubicin-induced renal inflammation in rats: Protective role of Plantago major
Objective: The aim of the present study was to evaluate the possible protective effect of Plantago major (P. major) extract against doxorubicin (DXR)-induced renal inflammation in rats. Materials and Methods: 80 male albino rats were randomly divided into 8 groups as follows: control, DXR, Ext (extract) 600, Ext1200, dexamethasone+DXR, vitamin E+DXR, Ext600+DXR, and Ext1200+DXR. Duration of the study was 35 days and DXR was intravenously injected on the 7th day of the experiment. Tumor necrosis factor-alpha (TNF-α) production and monocyte chemoattractant protein-1 (MCP-1) expression levels were assessed in the left kidney. Serum creatinine concentration and osmolarity were determined on the 1st, 14th, 21st, 28th and 35th days of the experiment. Results: DXR caused a significant increase in renal expression of MCP-1 and TNF-α production compared to control animals. Administration of dexamethasone, vitamin E and P. major extract significantly improved the expression of these inflammatory mediators compared to DXR group. Compared to day 1 in DXR group, serum osmolarity showed a significant increase on days 21, 28 and 35. Also, on these days, serum osmolarity in DXR group was significantly higher than that on the same days in control group. In Vit E+DXR and Ext 1200+DXR groups, there was no significant changes in serum osmolarity among different days of the study. However, in these groups, serum osmolarity on days 21, 28 and 35 showed a significant decrease compared to the same days in DXR group. Conclusion: Present results suggest that hydroethanolic extract of P. major protected renal tissue against DXR–induced renal inflammation.
https://ajp.mums.ac.ir/article_10396_a13b50990ab2ed89a21498ee795facf1.pdf
2018-03-01
179
187
10.22038/ajp.2018.10396
Plantago major
Doxorubicin
Vitamin E
Dexamethasone
Inflammation
Nazanin
Entezari Heravi
entezarin931@mums.ac.ir
1
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
AUTHOR
Sara
Hosseinian
hoseinians@mums.ac.ir
2
Department of physiology, School of medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Zohreh
Naji Ebrahimi Yazd
najiez921@mums.ac.ir
3
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
AUTHOR
Mohammad Naser
Shafei
shafeimn@mums.ac.ir
4
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Alireza
Ebrahimzadeh
ebrahimzadehba@mums.ac.ir
5
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Samira
Shahraki
shahrakis921@mums.ac.ir
6
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Zahra
Samadi
samadinz941@mums.ac.ir
7
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
AUTHOR
Fatemeh
Motejadded
motejadedf1@mums.ac.ir
8
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Farimah
Beheshti
beheshtif1@thums.ac.ir
9
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Reza
Mohebbati
mohebbatir931@mums.ac.ir
10
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Iran
AUTHOR
Soghra
Parhizgar
parhizgars1@mums.ac.ir
11
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
AUTHOR
Abolfazl
Khajavirad
khajavirada@mums.ac.ir
12
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Benchetrit S, Golan E, Podjarny E, Green J, Rashid G, Bernheim J. 2001. Low molecular weight heparin reduces proteinuria and modulates glomerular TNF-alpha production in the early phase of adriamycin nephropathy. Nephron, 87: 155-160.
1
Cummings J, Anderson L, Willmott N, Smyth JF. 1991. The molecular pharmacology of doxorubicin in vivo. Eur J Cancer Clin Oncol, 27: 532-535.
2
Deschenes G, Doucet A. 2000. Collecting Duct Na+/K+-ATPase Activity Is Correlated with Urinary Sodium Excretion in Rat Nephrotic Syndromes. J Am Soc Nephrol, 11: 604-615.
3
Havakhah S, Sadeghnia HR, Hadjzadeh MR, Mohammadian Roshan N, Hosseinzadeh H, Mohareri N, Khajavi Rad A. 2014. Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage. Iran J Basic Med Sci, 17: 986-992.
4
Hosseinian S, Khajavi Rad A, Ebrahimzadeh A, Soukhtanloo M, Sadeghnia HR, Shafei MN. 2017. Thymoquinone ameliorates renal damage in unilateral ureteral obstruction in rats. Pharmacol Rep, 69: 648-657.
5
Hosseinian S, Khajavi Rad A, Hadjzadeh MR, Mohammadian Roshan N, Shafiee S. 2016. The protective effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats. Avicenna J Phytomed, 6: 44-54.
6
Jamilah J, Sharifa A, Sharifah N. 2012. GC-MS analysis of various extracts from leaf of Plantago major used as traditional medicine. World Appl Sci J, 17: 67-70.
7
Katzung B, Masters S, Trevor A. 2012. Basic and clinical pharmacology, pp 1108, NewYork, McGrow-Hill.
8
Kim DR, Lee SY, Kim JS, Kim YG, Moon JY, Lee SH. 2017. Ameliorating Effect of Gemigliptin on Renal Injury in Murine Adriamycin-Induced Nephropathy. Biomed Res Int, 2017.
9
Lee VW, Harris DC. 2011. Adriamycin nephropathy: a model of focal segmental glomerulosclerosis. Nephrol, 16: 30-38.
10
Miraj S. 2016. A review study of pharmacological properties of plantago major l. Der Pharma Chemica, 8: 21-25.
11
Mohebbati, R, Shafei MN, Beheshti F, Soukhtanloo M, Mohammadian Roshan N, Anaeigoudari A, Parhizgar S, Hosseinian S, Khazdeir MR, Khajavi Rad A. 2017. Mixed Hydroalcoholic Extracts of Nigella sativa and Curcuma longa Improves Adriamycin-Induced Renal Injury in Rat. Saudi J Kidney Dis Transpl, 28:1270-1281.
12
Mohebbati R, Shafei MN, Soukhtanloo M, Roshan NM, Khajavi Rad A, Anaeigoudari A, Hosseinian S, Karimi S, Beheshti F. 2016. Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney. Avicenna J Phytomed, 6: 86-94.
13
Neale TJ, Rüger B, Macaulay H, Dunbar PR, Hasan Q, Bourke A. 1995. Tumor necrosis factor-alpha is expressed by glomerular visceral epithelial cells in human membranous nephropathy. Am J Pathol, 146: 1444-1454.
14
Orth SR, Ritz E. 1998. The nephrotic syndrome. New England J Med, 338: 1202-1211.
15
Okuda S, Oh Y, Tsuruda H, Onoyama K, Fujimi S, Fujishima M. 1986. Adriamycin-induced nephropathy as a model of chronic progressive glomerular disease. Kidney Int, 29: 502-510.
16
Parhizgar S, Hosseinian S, Hadjzadeh MR, Soukhtanloo M, Ebrahimzadeh Bideskan A, Mohebbati R, Naji Ebrahimi Yazd Z, Khajavi Rad A. 2016. Renoprotective Effect of Plantago Major Against Nephrotoxicity and Oxidative Stress Induced by Cisplatin. Iran J Kidney Dis, 10: 182-188.
17
Rangan GK, Wang Y, Tay YC, Harris DC. 2000. Cytokine gene expression in Adriamycin nephropathy: Effects of antioxidant nuclear factor κB inhibitors in established disease. Nephron, 86: 482-490.
18
Samuelsen AB. 2000. The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol, 71: 1-21.
19
Szalay CI, Erdélyi K, Kökény G, Lajtár E, Godó M, Révész C. 2015. Oxidative/nitrative stress and inflammation drive progression of doxorubicin-induced renal fibrosis in rats as revealed by comparing a normal and a fibrosis-resistant rat strain. PloS One, 10: e0127090.
20
Yamashita M, Yoshida T, Suzuki S, Homma K, Hayashi M. 2017. Podocyte-specific NF-κB inhibition ameliorates proteinuria in adriamycin-induced nephropathy in mice. Clin Exp Nephrol, 21: 16-26.
21
Zhang Y, Kong J, Deb DK, Chang A, Li YC. 2010. Vitamin D receptor attenuates renal fibrosis by suppressing the renin-angiotensin system. J Am Soc Nephrol, 21: 966-973.
22