Arbutin attenuates nephrotoxicity induced by gentamicin
Elnaz
Emadi
Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
author
Mahdi
Pouramir
Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
author
Maryam
Ghasemi-Kasman
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
author
Farideh
Feizi
Department of Anatomy, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
author
Sohrab
Halalkhor
Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
author
Ali Akbar
Moghadamnia
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
author
text
article
2021
eng
Objective: In this study, the impact of arbutin was examined in a gentamicin (GM)-induced nephrotoxicity model.Materials and Methods: Forty adult male Wistar rats were randomly assigned to five groups including control group; GM group, and three groups of GM+arbutin (25, 50 and 75 mg/kg). One day after the last injection of GM, creatinine, urea, carbonyl, thiobarbituric acid-reacting substance (TBARs), ferric reducing antioxidant power (FRAP) and 8-hydroxyguanosine levels were assessed in serum samples. Left and right kidneys were used for biochemical assays and histological evaluation, respectively.Results: Our data showed that the FRAP level (p<0.05), urea (p<0.001), creatinine (p<0.001), and 8-hydroxyguanosine (p<0.001) levels of serum samples, were increased in GM-treated rats compared to the controls. The serum levels of TBARS (p<0.001) and carbonyl increased in serum and renal tissue (p<0.001) of GM-treated animals. Conversely, arbutin attenuated serum creatinine, urea and 8-hydroxyguanosine, and TBARS (p<0.001). Administration of arbutin significantly decreased carbonyl levels in serum and renal tissue samples (p<0.001). Furthermore, the levels of FRAP increased in the serum (p<0.01) and renal tissue samples (p<0.001) of arbutin-treated animals. Histological staining showed that arbutin significantly inhibits kidney damages.Conclusion: Our data suggest that arbutin attenuates GM-induced nephrotoxicity through its free radicals-scavenging activity.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
210
217
https://ajp.mums.ac.ir/article_16776_5070343cae4a1ed471f0578c499c2418.pdf
dx.doi.org/10.22038/ajp.2020.16776
The effect of royal jelly and silver nanoparticles on liver and kidney inflammation
Hossein
Pourmobini
Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences
author
Mohammad
Kazemi-arababadi
Department of Immunology, Faculty of Medicine, Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Mohammad Reza
Salahshoor
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Shiva
Roshankhah
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Mohammad Mohsen
Taghavi
Department of Anatomy, Faculty of Medicine, Social Determinants of Health Research center Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Zahra
Taghipour
Department of Anatomy, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Ahmad
Shabanizadeh
Department of Anatomy, Faculty of Medicine, Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
text
article
2021
eng
Objective: Royal jelly (RJ) is a honey bee product for which, anti-inflammatory properties were shown in vitro. Nanoparticles, including nano-silver (NS), are plausible inflammation inducers that act by activation of immune cells and consequent production of pro-inflammatory cytokines. This project aimed to explore immunomodulatory effects of royal jelly and nano-silver on the kidney and liver.Materials and Methods: In this project, 40 male rats were grouped as follows: 10 rats as controls, 10 rats treated with RJ; 10 rats treated with both NS and RJ and 10 rats treated with NS. Liver and kidney interleukin (IL)-1β, -2, -6, and -33 levels were determined using commercial ELISA kits.Results: RJ reduced kidney IL-6 levels in comparison to control and NS--RJ groups. RJ and NS reduced kidney and liver IL-1β levels. Kidney IL-33 levels were decreased in the RJ and nano-silver groups in comparison to the NS--RJ group.Conclusion: Based on this study, it may be concluded that RJ together with NS can play anti-inflammatory roles and may affect the function of immune cells.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
218
223
https://ajp.mums.ac.ir/article_17045_f53b8a093ef7f2943c13de1dda24ab1c.pdf
dx.doi.org/10.22038/ajp.2020.17045
Antiglycation and antitumoral activity of Tribulus terrestris dry extract
Célia Cristina Malaguti
Figueiredo
São Paulo State University (UNESP), Institute of Chemistry, Araraquara, São Paulo, Brazil
author
Amanda
Gomes
São Paulo State University (UNESP), Institute of Chemistry, Araraquara, São Paulo, Brazil
author
Filipe
Granero
São Paulo State University (UNESP), Institute of Chemistry, Araraquara, São Paulo, Brazil
author
João Luiz
Bronzel Junior
São Paulo State University (UNESP), Institute of Chemistry, Araraquara, São Paulo, Brazil
author
Luciana
Silva
Fundação Educacional do Município de Assis (FEMA), Assis, São Paulo, Brazil
author
Ana Lúcia Tasca Gois
Ruiz
University of Campinas (UNICAMP), Faculty of Pharmaceutical Sciences, Campinas, São Paulo, Brazil
author
Regildo Márcio Gonçalves da
Silva
São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Department of Biotechnology, Laboratory of Herbal Medicine and Natural Products, Assis, São Paulo, Brazil
author
text
article
2021
eng
Objective: Investigation of the antiglycation and antitumoral potential of standardized and saponins-enriched extracts of Tribulus terrestris herbal medicine.Materials and Methods: The procedures for the evaluation of the antiglycation activity of the standardized (TtSE) and saponins-enriched (TtEE) extracts of T. terrestris were: determination of relative mobility in electrophoresis (RME), free amino groups using OPA method and advanced glycation end-products (AGEs) fluorescence. Antioxidant activity was determined by DPPH radical scavenging test. In vitro antitumor activity of TtSE and TtEE was evaluated in human tumor cell lines.Results: The results were obtained by antiglycation tests (RME, OPA method and AGEs fluorescence determination), using BSA as protein and ribose as glycation agent, and antioxidant assay (DPPH test); it was verified that both extracts of T. terrestris have antiglycation and antioxidant activity. In addition, the extracts were able to induce death of more than 50% of human tumor cell lines.Conclusion: The present study showed that standardized and saponins-enriched extracts of T. terrestris herbal medicine present antiglycation and antioxidant and antiproliferative action in human tumor cells lines. The saponins-enriched extract proved a greater antiglycation and antioxidant activity in comparison to the standardized type.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
224
237
https://ajp.mums.ac.ir/article_16957_07703119bfdc6a98f53adc1aaf1a995a.pdf
dx.doi.org/10.22038/ajp.2020.16957
Protective effect of Artemisia absinthium on 6-hydroxydopamine-induced toxicity in SH-SY5Y cell line
Roghayeh
Rashidi
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ahmad
Ghorbani
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hasan
Rakhshandeh
Department of Pharmacology and Medicinal plant Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
author
Seyed Hadi
Mousavi
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2021
eng
Objective: Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons. Several experimental studies have shown neuroprotective and antioxidant effects for Artemisia absinthium. The present study was designed to assess the effect of A. absinthium on 6-hydroxydopamine (6-OHDA)-induced toxicity in SH-SY5Y cells.Materials and Methods: SH-SY5Y cells were treated with ethanolic extract of A. absinthium for 24 hr and then, exposed to 6-OHDA (250 μM) for another 24 hr. MTT (3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyl tetrazolium bromide) assay was used for evaluation of cell viability. Moreover, the rate of apoptosis was measured using propidium iodide (PI) staining. The amount of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) was also measured using 2’, 7’–dichlorofluorescin diacetate (DCFDA) fluorometric method. Determination of glutathione (GSH) and superoxide dismutase (SOD) activity was done by colorimetric assay using DTNB [5, 5′-Dithiobis (2-nitrobenzoic acid)] and pyrogallol respectively.Results: While 6-OHDA significantly increased ROS and apoptosis (p<0.001), the extract of A. absinthium significantly reduced ROS and cell apoptosis at concentrations ranging from 6.25 to 25 μg/mL (p<0.01 and p<0.001 respectively). Also, the extractsignificantly reduced MDA level in comparison with 6-OHDA (p<0.001). The GSH level and SOD activity were increased by the extract.Conclusion: Findings of the current study showed that A. absinthium exerts it effect throughinhibiting oxidative stress parameters and it can be considered a promising candidate to be used in combination with the conventional medications for the treatment of neurodegenerative disorders, such as Parkinson's disease.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
238
246
https://ajp.mums.ac.ir/article_16426_91c6ab4ccd512106aac23d2788dc97b0.pdf
dx.doi.org/10.22038/ajp.2020.16426
Abscisic acid interplays with PPARγ receptors and ameliorates diabetes-induced cognitive deficits in rats
Razieh
Kooshki
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
author
Akbar
Anaeigoudari
Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran
author
Mehdi
Abbasnejad
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
author
Khadijeh
Askari-Zahabi
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
author
Saeed
Esmaeili-Mahani
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2021
eng
Objective: This study intended to evaluate if central administration of abscisic acid (ABA) alone or in combination with GW9662, a peroxisome proliferator–activated receptor γ (PPAR-γ) antagonist, could modulate learning and memory as well as hippocampal synaptic plasticity in a rat model of streptozotocin (STZ)–induced diabetes.Materials and Methods: Intraperitoneal injection of STZ (65 mg/kg) was used to induce diabetes. Diabetic rats were than treated with intracerebroventricular (i.c.v.) administration of ABA (10, 15 and 20 µg/rat), GW9662 (3 µg/rat) or GW9662 (3 µg/rat) plus ABA (20 µg/rat).Animals’ spatial and passive avoidance learning and memory performances were assessed by Morris water maze (MWM) and shuttle box tasks, respectively. Further, in vivo electrophysiological field recordings were assessed in the CA1 region.Results: STZ diabetic rats showed diminished learning and memory in both MWM and shuttle box tasks. The STZ-induced memory deficits were attenuated by central infusion of ABA (10 and 20 µg/rat). Besides, STZ injection impaired long-term potentiation induction in CA1 neurons that was attenuated by ABA at 20 μg/rat. Central administration of GW9662 (3 µg/rat) alone did not modify STZ-induced spatial and passive avoidance learning and memory performances of rats. Further, GW9662 prevented ABA capacity to restore learning and memory in behavioral and electrophysiology trials.Conclusion: Altogether, ABA ameliorates cognitive deficits in rats via activation of PPAR-γ receptor in diabetic rats.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
247
257
https://ajp.mums.ac.ir/article_16777_2e9ae72aa244e338371435188afd1dc7.pdf
dx.doi.org/10.22038/ajp.2020.16777
Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines
Fatemeh
Karimi Dermani
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran
author
Massoud
Saidijam
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
author
Rezvan
Najafi
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran
author
Shirin
Moradkhani
Department of pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
author
Zahra
Mohammadzaheri
Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan,Iran
author
Negar
Beiranvand
Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
author
Samane
Mohammadi
Department of pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
author
Noushin
Shabab
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran .
author
Ramazan
Kalvandi
Natural Resources Department, Hamadan Agricultural and Natural Resources Research and Education Center, AREEO, Hamadan, Iran
author
Fatemeh
Zeraati
Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
author
text
article
2021
eng
Objective: Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. Cuscuta chinensis a traditional Iranian medicinal herb, has biological properties including anticancer, anti-aging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of C. chinensis on prostate (PC3) and breast (MCF7) cancer cell lines were investigated.Materials and Methods: In the current study, we investigated treatment of PC3 cells with different concentrations of C. chinensis (0, 100, 200, 300, 400, and 500 µg/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 µg/ml) of C. chinensis for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (Bax), B-cell lymphoma 2 (Bcl2), Cysteine-aspartic proteases (Caspase3) and Phosphatase and tensin homolog (PTEN) were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect apoptosis.Results: C. chinensis decreased PC3 and MCF7 cells viability in a dose- and time-dependent manner (p BAX/Bcl2 ratio, Caspase3 and PTEN increased in C. chinensis-treated cells compared to the control group. C. chinensis induced apoptosis (p <0.001) and LDH activity (p Conclusion: Our findings suggest that C. chinensis extract is able to inhibit proliferation and induce apoptosis in PC3 and MCF7 cell lines. Therefore, C. chinensis extract exerts antitumor activity against cancer cells.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
258
268
https://ajp.mums.ac.ir/article_16902_98edd3e0a693c715c3bfb3319058c29f.pdf
dx.doi.org/10.22038/ajp.2020.16902
Protective effects of quercetin against hyperglycemia-induced oxidative stress in hepatic HepG2 cell line
Amir
Yarahmadi
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
author
Mostafa
Moradi Sarabi
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Faculty of Medicine, Khorramabad, Iran.
author
Ahmad
Sayahi
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Faculty of Medicine, Khorramabad, Iran.
author
Fatemeh
Zal
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
author
text
article
2021
eng
Objective: Hyperglycemia is a severe consequence of diabetes mellitus (DM). Throughinduction of oxidative stress, it plays a major role in the pathogenesis of several complications in DM. Therefore, new strategies and antioxidants should be implemented inthe treatment of DM. Quercetin is a flavonoid with strong antioxidant capacity found dominantly in vegetables, fruits, leaves, and grains. The current study aimed to investigate quercetin protective effects under D-glucose-induced oxidative stress by assessing antioxidant defense enzymes inHepG2 cells as an in vitro model.Materials and Methods: HepG2 cells were cultured with different concentrations of D-glucose (5.5, 30 and 50 mM) and/or 25 μM quercetin for 48 and 72 hr, respectively. The effect of treatments on cellular integrity, antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) activity, andcellular levels of glutathione (GSH) and malondialdehyde (MDA) wasdetermined.Results: D-glucose had various effects on intracellular antioxidant defense atdifferent doses and time-points and quercetin could attenuate oxidative stress and modulate antioxidant defenses.Conclusion: The results of this study indicated that flavonoid quercetin could be proposed as an agent protecting hepatic HepG2 cells against oxidative stress associated with hyperglycemia.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
269
280
https://ajp.mums.ac.ir/article_16913_59070034c2507837547fc935789599b4.pdf
dx.doi.org/10.22038/ajp.2020.16913
Osteogenic induction of menstrual blood mesenchymal stem cell by different Ferula species extracts
Razieh
Dalirfardouei
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
author
Elahe
Mahdipour
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mehrdad
Iranshahi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
Khadijeh
Jamialahmadi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2021
eng
Objective: Ferula spp. have many applications in complementary medicine and are recognized as the most important sources of natural products for bone health and regeneration especially in postmenopausal women. Therefore, the aim of this study was to investigate the effects of the extracts from three Ferula species on proliferation and osteogenesis potential of human menstrual blood-derived mesenchymal stem cells (MenSCs).Materials and Methods: The possible cytotoxic activity of three members of Ferula spp. (at concentrations of 5 to 100 μg/ml) was determined using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay. Alkaline phosphatase (ALP) activity assay, Alizarin Red-S staining, and the expression analysis of an osteoblastic gene were performed to evaluate osteogenic differentiation potential.Results: The extracts of F. flabelliloba and F. szowitsiana decreased the viability and growth of MenSCs while F. foetida increased the proliferation of cells after 72 hr incubation. Treatment of MenSCs with selected plant extracts revealed that F. foetida and F. szowitsiana could enhance the osteogenic potential of MenSCs in terms of ALP activity. The Runx-2 expression in the presence of F. foetida was significantly greater than observed following treatment with 17β-estradiol (as positive control).Conclusion: The results of this study suggest that F. foetida and F. szowitsiana may have therapeutic values as a nutraceutical with respect to their considerable influence on osteogenic potential of mesenchymal stem cells.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
281
291
https://ajp.mums.ac.ir/article_16956_0aa549fc1a5a554d4cec8f381930e50e.pdf
dx.doi.org/10.22038/ajp.2020.16956
Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome
Zoleykha
Feizollahi
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
Homa
Mohseni Kouchesfahani
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
Hanieh
Jalali
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
Delaram
Eslimi Esfahani
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
Abbas
Sheikh Hosseini
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
author
text
article
2021
eng
Objective: Polycystic ovary syndrome (PCOS) is an endocrine system disruption that affects 6-10% of women. Some studies have reported the effect of Vitex agnus-castus (Vitagnus) on the hypothalamic-pituitary-gonad axis (HPG). This study was conducted to investigate Vitagnus effect on the expression of kisspeptin gene in a rat model of PCOS.Materials and Methods: Thirty-two female rats were distributed into: control, Vitagnus-treatment (365 mg/kg for 30 days), PCOS (Letrozole for 28 days) and PCOS animals treated with Vitagnus (30 days of Vitagnus after PCOS induction). At the end of the treatments, serum and ovaries were collected for analysis. Expression level of KISS-1 gene in the hypothalamus was investigated, using Real-Time-PCR.Results: In the PCOS group compared to control, FSH, progesterone and estradiol levels were decreased, whereas testosterone and LH levels were significantly increased. No significant changes were observed in the Vitagnus-treated animals in compare to control. However, Vitagnus treatment in the PCOS group, resulted in a raise in progesterone, estrogen and FSH levels and a reduction in the levels of testosterone and LH. Quantitative gene expression analysis showed that PCOS induction resulted in over-expression of KISS-1 gene, however, Vitagnus treatment reduced this up-regulated expression to normal level.Conclusion: In conclusion, our results indicated that Vitagnus extract inhibited downregulation of KISS-1 gene in the hypothalamus of PCOS rats. Because of the master role of kisspeptin in adjusting the HPG axis, Vitagnus is likely to show beneficial effects in the treatment of PCOS via regulation of kisspeptin expression. This finding indicates a new aspect of Vitagnus effect and may be considered in its clinical applications.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
292
301
https://ajp.mums.ac.ir/article_17046_3246f9a4bd7c5d149fc733563ab3b3d2.pdf
dx.doi.org/10.22038/ajp.2020.17046
Cinnamomum zeylanicum extract has antidepressant-like effects by increasing brain-derived neurotrophic factor (BDNF) and its receptor in prefrontal cortex of rats
Mona
Aryanezhad
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
author
Mohammad
Abdi
Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
author
Sabrieh
Amini
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
author
Kambiz
Hassanzadeh
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
author
Elham
Valadbeigi
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
author
Kaveh
Rahimi
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
author
Esmael
Izadpanah
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
author
Mohammad Raman
Moloudi
Neurosciences Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
author
text
article
2021
eng
Objective: Depression is one of the most common mood disorders. Considering the evidence on the effect of Cinnamomum on mood disorders, this study investigatedthe effect of hydroalcoholic extract of Cinnamomum (HEC) in an animal model of depression.Materials and Methods: Thirty-two male rats were selected and divided into four groups (n=8) including: control, depressed, and depressed treated with200 and 400 mg/kg HEC. Depression induction protocol was conducted in all groups except for the control group. Sucrose preference test (SPT) and forced swimming test (FST) were done to analyze the depression score. After four weeks, the animals brain cortex was removed and BDNF protein and tyrosine receptor kinase B (TrkB) gene expression levels were determined by ELISA and Real Time PCR, respectively.Results: The results of this study showed that 400 mg/kg of HEC increased the tendency to drink the sucrose solution. Furthermore, immobility time significantly increased in the depressed group compared to the control group while it was attenuated by administration of 400 mg/kg extract on the 28th day versus the depressed group. Also the extract at both doses increased swimming time compared to the depressed group. In addition, an increase in the BDNF protein and TrkB gene expression levels was observed in the prefrontal cortex of the treatment groups.Conclusion: We found that HEC ameliorated depression symptoms in rats and these effects were probably due to an increase in BDNF proteins and its receptor, TrkB, gene expressions in the prefrontal cortex.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
11
v.
3
no.
2021
302
313
https://ajp.mums.ac.ir/article_17146_1cd7780d36c503bf93098367a9fc7981.pdf
dx.doi.org/10.22038/ajp.2020.17146