Tiliacora triandra (Colebr.) Diels leaf extract enhances spatial learning and learning flexibility, and prevents dentate gyrus neuronal damage induced by cerebral ischemia/reperfusion injury in mice

Document Type : Original Research Article

Authors

1 Physiology Division, Animal Toxicology and Physiology Specialty Research Unit (ATPRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand

2 Animal Toxicology and Physiology Specialty Research Unit (ATPRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand

3 Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Hiranruchi, Dhonburi Bangkok, Thailand

4 Neuroscience Unit, Department of Physiology, Faculty of Medicine Siriraj, Siriraj Hospital, Mahidol University, Bangkok, Thailand

Abstract

Objective: The present study investigated the effects of a local Thai vegetable, Tiliacora triandra (Colebr.) Diels, also known as Yanang, against cerebral ischemia/reperfusion injury in mice.
Materials and Methods: Thirty male ICR mice were divided into three experimental groups of BLCCAO + 10% Tween 80, BLCCAO + T. triandra 300 mg/kg, and BLCCAO + T. triandra 600 mg/kg. Cerebral ischemia/reperfusion was induced by three minutes of bilateral common carotid artery occlusion (BLCCAO) followed by 18 days of reperfusion. Leaf extract was administered orally 24 hours after arterial occlusion and continued for 18 consecutive days. Cognitive abilities were evaluated using the Morris water maze. Histological analysis was conducted in the dorsal hippocampus subregions CA1, CA3, and DG and white matter regions (the corpus callosum, internal capsule, and optic tract) using 0.1 % cresyl violet and 0.1% Luxol fast blue staining.
Results: Results showed that T. triandra leaf extract at the doses of 300 and 600 mg/kg significantly enhanced spatial learning, and learning flexibility, and prevented neuronal death in the DG of mice following ischemia/reperfusion.
Conclusion: T. triandra leaf extract enhanced spatial learning, and learning flexibility, and prevented DG neuronal death in a mice model of cerebral ischemia/reperfusion.

Keywords

Main Subjects


Boonsong P, Laohakunjit N, Kerdchoechuen O. 2009. Identification of polyphenolic  compounds and colorants from Tiliacora triandra (Diels) Leaves. Agr Sci J, 40: 13-16.
Broadbent NJ, Squire LR, Clark RE. 2004. Spatial memory, recognition memory, and the hippocampus. Proc Natl Acad Sci U S A, 101: 14515-14520.
Ehninger D, Kempermann G. 2006. Paradoxical effects of learning the Morris water maze on adult hippocampal neurogenesis in mice may be explained by a combination of stress and physical activity. Genes Brain Behav, 5: 29-39.
Eisch AJ. 2002. Adult neurogenesis: implications for psychiatry, Progress in Brain Research. Elsevier, pp. 315-342.
Farhoosh R, Johnny S, Asnaashari M, Molaahmadibahraseman N, Sharif A. 2016. Structure antioxidant activity relationships of o-hydroxyl, o-methoxy, and alkyl ester derivatives of p-hydroxybenzoic acid. Food Chem, 194: 128-134.
Hou ST, MacManus JP, Kwang WJ. 2002. Molecular mechanisms of cerebral ischemia-induced neuronal death, International Review of Cytology, pp. 93-148, Academic Press
Kaewpiboon C, Winayanuwattikun P, Yongvanich T, Phuwapraisirisan P, Assavalapsakul W. 2014. Effect of three fatty acids from the leaf extract of Tiliacora triandra on P-glycoprotein function in multidrug-resistant A549RT-eto cell line. Pharmacogn Mag 10: 549-556.
Khan SA, Chatterjee SS, Kumar V. 2016. Low dose aspirin like analgesic and anti-inflammatory activities of mono-hydroxybenzoic acids in stressed rodents. Life Sciences, 148: 53-62.
Logue SF, Gould TJ. 2014. The neural and genetic basis of executive function: Attention, cognitive flexibility, and response inhibition. Pharmacol Biochem Be, 123:45-54.
Luikart BW, Perederiy JV, Westbrook GL. 2012. Dentate gyrus neurogenesis, integration and microRNAs. Beh Brain Res, 227: 348-355.
Murakami K, Kondo T, Kawase M, Chan PH. 1998. The development of a new mouse model of global ischemia: focus on the relationships between ischemia duration, anesthesia, cerebral vasculature, and neuronal injury following global ischemia in mice. Brain Res, 780: 304-310.
Parihar MS, Hemnanai T. 2003. Phenolic antioxidants attenuate hippocampal neuronal cell damage against kainic acid induced exitotoxicity. J Biosci, 28: 121-128.
Paxinos G, Franklin K. 2008. The Mouse Brain in Stereotaxic Coordinates (3rd Ed), pp. 197-215, Academic Press.
Phillis JW, O'Regan MH, Donard SD. 2002. Energy utilization in the ischemic/reperfused brain, International Review of Neurobiology, pp. 377-414, Academic Press.
Phunchango N, Wattanathorn J, Chaisiwamongkol K. 2015. Tiliacora triandra, an Anti-Intoxication Plant, Improves Memory Impairment, Neurodegeneration, Cholinergic Function, and OCidative Stress in Hippocampus of Ethanol Dependence Rats. Oxid Med Cell Longev, 2015: 9.
Ragozzino ME. 2007. The Contribution of the Medial Prefrontal Cortex, Orbitofrontal Cortex, and Dorsomedial Striatum to Behavioral Flexibility. Ann N Y Acad Sci, 1121: 355-375.
Ragozzino ME, Jih J, Tzavos A. 2002. Involvement of the dorsomedial striatum in behavioral flexibility: role of muscarinic cholinergic receptors. Brain Res, 953: 205-214.
Singthong J, Oonsivilai R, Oonmetta-Aree J, Ningsanond S. 2014. Bioactive compounds and encapsulation of Yanang (Tiliacora triandra) leaves. Afr J Tradit Complement Altern Med, 11: 76-84.
Sireeratawong S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N, Jaijoy K. 2008. Acute and subchronic toxicity study of the water extract from Tiliacora triandra (Colebr.) Diels in rats. Songklanakarin J Sci Technol, 30: 611-619.
Sureram S, Senadeera SPD, Hongmanee P, Mahidol C, Ruchirawat S, Kittakoop P. 2012. Antimycobacterial activity of bisbenzylisoquinoline alkaloids from Tiliacora triandra against multidrug-resistant isolates of Mycobacterium tuberculosis. Bioorg Med Chem Lett, 22: 2902-2905.
Taupin P. 2006. Neurogenesis in the adult central nervous system. Comptes Rendus Biologies, 329: 465-475.
Thong-asa W, Tilokskulchai K. 2014. Neuronal damage of the dorsal hippocampus induced by long-term right common carotid artery occlusion in rats. Iran J Basic Med Sci, 17: 220 - 226.
Treves A, Tashiro A, Witter MP, Moser EI. 2008. What is the mammalian dentate gyrus good for? Neuroscience, 154: 1155-1172.
White BC, Sullivan JM, DeGracia DJ, O' Neil BJ, Neumar RW, Grossman LI, Rafols JA, Krause GS. 2000. Brain ischemia and reperfusion: molecular mechanisms of neuronal injury. J Neurol Sci, 179: 1-33.