TY - JOUR ID - 13556 TI - Effects of aqueous extract of Hyssopus officinalis on seizures induced by pentylenetetrazole and hippocampus mRNA level of iNOS in rats JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Gholami, Masoumeh AU - Jafari, Faranak AU - Baradaran, Zahra AU - Amri, Jamal AU - Azhdari-zarmehri, Hassan AU - Sadegh, Mehdi AD - Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. AD - Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran AD - Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran AD - Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. Y1 - 2020 PY - 2020 VL - 10 IS - 3 SP - 213 EP - 221 KW - Anticonvulsive KW - Epilepsy KW - Aqueous extract KW - Nitric oxide KW - Hyssopus officinalis DO - 10.22038/ajp.2019.13556 N2 - Objective: We examined the effectiveness of Hyssopus officinalis (hyssop) aqueous extract on pentylenetetrazole (PTZ)-induced acute seizures and the hippocampus iNOS (induciblenitric oxide synthases) gene expression as a potential mediator of the effects. Materials and Methods: Adult male Wistar rats were used. Tonic-clonic seizures were induced by intraperitoneal (i.p.) injection of PTZ (80 mg/kg) then behavioral profile during 30 min was characterized by stages defined as seizure scores. Hyssop extract were prepared and injected (i.p.) 15 minutes before the seizure induction at three doses 50, 100 and 200 mg/kg. Experimental groups were as below: (1) saline+PTZ (n=5); (2) Hyssop 50mg/kg+PTZ (n=10); (3) Hyssop 100mg/kg+PTZ (n=10); (4) Hyssop 200 mg/kg+PTZ (n=8). Two hours after the experimental procedure, all aniĀ­mals were decapitated, brain was removed and right hippocampus was quickly dissected. After total RNA extraction and cDNA synthesis quantitative PCR were used for gene expression of iNOS. Results: Our results showed significant increase (p<0.05) in latency to reach stages 5 and 6 of tonic-clonic seizure at dose 100 mg/kg hyssop extract. In addition, this dose caused significant increase in the gene expression of iNOS in the hippocampus. Conclusion: It seems a 100 mg/kg dose of hyssop extract might have anticonvulsant effects. However, these anticonvulsant effects might not occur through the iNOS gene expression. UR - https://ajp.mums.ac.ir/article_13556.html L1 - https://ajp.mums.ac.ir/article_13556_a4795f86e3af2285b30bf306c3d8fa4b.pdf ER -