TY - JOUR ID - 16 TI - Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Alam, Badrul AU - Akter, Fahima AU - Parvin, Nahida AU - Sharmin Pia, Rashna AU - Akter, Sharmin AU - Chowdhury, Jesmin AU - Sifath-E-Jahan, Kazi AU - Haque, Ekramul Haque AD - Department of Pharmacy, Atish Dipankar University of Science & Technology, Dhaka, Bangladesh AD - Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh Y1 - 2013 PY - 2013 VL - 3 IS - 2 SP - 112 EP - 125 KW - Analgesic KW - Antioxidant KW - Anti-inflammatory KW - Piper betle DO - 10.22038/ajp.2013.16 N2 - Objective:The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities ofthe methanolic extract of Piper betle leaves (MPBL). Materials and Methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesic activity of MPBL was evaluated by hot plate, writhing, and formalin tests. Total phenolic and flavonoids content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO-) as well as  inhibition of total ROS generation, and assessment of reducing power were used to evaluate antioxidant potential of MPBL. Results: The extract of MPBL, at the dose of 100 and 200 mg/kg, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose-dependent manner. The same ranges of doses of MPBL caused significant (p<0.05) inhibition of carrageenan-induced paw edema after 4 h in a dose-dependent manner.  In DPPH, ONOO-, and total ROS scavenging method, MPBL showed good antioxidant potentiality with the IC50 value of 16.33±1.02, 25.16±0.61 , and 41.72±0.48 µg/ml, respectively with a significant (p<0.05) good reducing power.   Conclusion: The findings of the study suggested that MPBLhas strong analgesic, anti-inflammatory, and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality.   UR - https://ajp.mums.ac.ir/article_16.html L1 - https://ajp.mums.ac.ir/article_16_54cb0f98a7ee0e5768a3b2253dfac9cb.pdf ER -