A comparison of the effects of Portulaca oleracea seeds hydro-alcoholic extract and Vitamin C on biochemical, hemodynamic and functional parameters in cardiac tissue of rats with subclinical hyperthyroidism

Document Type: Original Research Article

Authors

1 Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

3 Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective: The present study was performed to evaluate the effects of hydro-alcoholic extract of Portulaca oleracea (P. oleracea) seeds and Vitamin C on biochemical and hemodynamic parameters in cardiac tissue of rats with subclinical hyperthyroidism.
Materials and Methods: Forty eight male rats were divided into six groups of 8 and treated for 4 weeks. T4 group received daily injection of levothyroxine sodium (20 μg/kg) and control group was given daily injection of saline. T4-Po groups were given T4 plus 100, 200, and 400 mg/kg of P. oleracea seeds extract in drinking water daily. T4-Vit C group received T4 plus daily injection of Vitamin C (100 mg/kg). At the end of the experiment, body weight, serum free T4 level, left ventricular developed pressure (LVDP), malondialdehyde (MDA) and total thiol levels were measured.
Results: Free T4 levels were increased in all groups that were treated with T4. Weight gain was decreased in T4 and T4-Po100 groups compared to control group (p Conclusion: The results showed that P. oleracea extract has a protective effect on cardiac dysfunction due to subclinical hyperthyroidism induced by levothyroxine sodium in rats.

Keywords

Main Subjects


Aghili MH. 1992. Makhzan-al-Advia, pp: 227-228, Tehran.

Bielecka-Dabrowa A, Mikhailidis DP, Rysz J, Banach M. 2009. The mechanisms of atrial fibrillation in hyperthyroidism. Thyroid Res, 2: 1.

Biondi B, Palmieri EA, Lombardi G, Fazio S. 2002. Effects of subclinical thyroid dysfunction on the heart. Ann Intern Med, 137: 904-914.

Boskabady MH, Hashemzehi M, Khazdair MR, Askari VR. 2016. Hydro-ethanolic Extract of Portulaca oleracea Affects Beta-adrenoceptors of Guinea Pig Tracheal Smooth Muscle. Iran J Pharm Res, 15: 867.

Chan K, Islam MW, Kamil M, Radhakrishnan RZakaria MNHabibullah MAttas A. 2000. The analgesic and anti-inflammatory effects of

Portulaca oleracea L. subsp. sativa (Haw.) Celak. J Ethnopharmacol, 73: 445-451.

Chen Y-F, Weltman NY, Li X, Youmans S, Krause D, Gerdes AM. 2013. Improvement of left ventricular remodeling after myocardial infarction with eight weeks L-thyroxine treatment in rats. J Transl Med, 11: 40.

Dos Reis-Lunardelli EA, Castro CC, Bavaresco C, 2007. Effects of thyroid hormones on memory and on Na+, K+-ATPase activity in rat brain. Curr Neurovasc Res, 4: 184-193.

Dweck AC. 2013. Purslane (P. oleracea)-the global panacea. Perso Care Mag, 2: 7-15.

Ellman GL. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys, 82: 70-77.

Fazio S, Palmieri EA, Lombardi G, Biondi B. 2004. Effects of thyroid hormone on the cardiovascular system. Recent Prog Horm Res, 59: 31-50.

Gao C, Li T, Liu J, Guo Q, Tian L. 2015. Endothelial functioning and hemodynamic parameters in rats with subclinical hypothyroid and the effects of thyroxine replacement. PloS one, 10:e0131776.

Gredilla R, Barja G, López-Torres M. 2001. Thyroid hormone-induced oxidative damage on lipids, glutathione and DNA in the mouse heart. Free Radic Res, 35: 417-425.

Hajzadeh M, Rakhshandeh H, Esmaeilizadeh M, Ghorbani A. 2004. Analgesic and anti-inflammatory effects of Portolaca oleracea extracts in mice & rat. Faslnamahi Kumish, 5: 113-120.

Hashemzehi M, Khazdair M, Kiyanmehr M, Askari V, Boskabady M. 2016. Portulaca olerace Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle. Indian J Pharm Sci, 78: 388-394.

Jacob RA, Sotoudeh G. 2002. Vitamin C function and status in chronic disease. Nutr Clin Care, 5: 66-74.

Janero DR. 1990. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9: 515-540.

Karimi G, Khoei A, Omidi A, 2010. Protective effect of aqueous and ethanolic extracts of Portulaca oleracea against cisplatin induced nephrotoxicity. Iran J Basic Med Sci, 13: 31-35.

Karmisholt J, Andersen S, Laurberg P. 2011. Weight loss after therapy of hypothyroidism is mainly caused by excretion of excess body water associated with myxoedema. J Clin Endocrinol Metab, 96: E99-E103.

Kaveh M, Eidi A, Neamati A, Boskabady MH. 2017. Modulation of lung inflammation and immune markers in asthmatic rats treated by Portulaca oleracea. Avicenna J Phytomed: 1-11.

Li X, Long Y, Fang X, Liu X. 2008. Effect of vitamin C and E on antioxidative enzyme, NOS activity and NO contents in hippocampus of rats with lead poisoning. Zhejiang Da Xue Xue Bao Yi Xue Ban, 37: 189-192.

Liao H-F, Chen Y-Y, Liu J-J, 2003. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem, 51: 7907-7912.

Lim Y, Quah E. 2007. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem, 103: 734-740.

Mamoun Ma, Rizvi Hsmta, Qazi Sm. 2015. Protective Effect of Vitamin C on Body Weight of Albino Rats with Lead Toxicity. Pak J Med Health Sci, 9

Marriott ML, McNeill JH. 1983. Effect of thyroid hormone treatment on responses of the isolated working rat heart. Can J Physiol Pharmacol, 61: 1382-1390.

Okafor IA, Ezejindu DN. 2014. Phytochemical studies on Portulaca oleracea (purslane) plant. GJBAHS, 3: 132-136.

Okwuasaba F, Ejike C, Parry O. 1987. Effects of extracts of Portulaca oleracea on skeletal muscle in vitro. J Ethnopharmacol, 21: 55-63.

Parry O, Okwuasaba F, Ejike C. 1988. Effect of an aqueous extract of Portulaca oleracea leaves on smooth muscle and rat blood pressure. J Ethnopharmacol, 22: 33-44.

Pijl H, De Meijer P, Langius J, 2001. Food choice in hyperthyroidism: potential influence of the autonomic nervous system and brain serotonin precursor availability. J Clin Endocrinol Metab, 86: 5848-5853.

Sultana A, Rahman K. 2013. Portulaca oleracea Linn. A global Panacea with ethno-medicinal and pharmacological potential. Int J Pharm Pharm Sci, 5: 33-39.

Sun H, He X, Liu C,  2016. Effect of Oleracein E, a Neuroprotective Tetrahydroisoquinoline, on Rotenone-Induced Parkinson’s Disease Cell and Animal Models. ACS Chem Neurosci. 8: 155-164.

Toft AD. 2001. Subclinical hyperthyroidism. N Engl J Med, 345: 512-516.

Venditti P, Balestrieri M, Di Meo S, De Leo T. 1997. Effect of thyroid state on lipid peroxidation, antioxidant defences, and susceptibility to oxidative stress in rat tissues. J Endocrinol, 155: 151-157.

Venditti P, Di Meo S. 2006. Thyroid hormone-induced oxidative stress. Cell Mol Life Sci, 63: 414-434.

Wu C-y, Liu B, Wang H-l, Ruan D-y. 2011. Levothyroxine rescues the lead-induced hypothyroidism and impairment of long-term potentiation in hippocampal CA1 region of the developmental rats. Toxicol Appl Pharmacol, 256: 191-197.

Yang Z, Liu C, Xiang L, Zheng Y. 2009. Phenolic alkaloids as a new class of antioxidants in Portulaca oleracea. Phytother Res, 23: 1032-1035.

Yazici I, Türkan I, Sekmen AH, Demiral T. 2007. Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environ Exp Bot, 61: 49-57.

Yen GC, Chen HY, Peng HH. 2001. Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants. Food Chem Toxicol, 39: 1045-1053.

Yu D, Zhou H, Yang Y, 2015. The bidirectional effects of hypothyroidism and hyperthyroidism on anxiety-and depression-like behaviors in rats. Horm Behav, 69: 106-115.

Zhang PY, Xu X, Li XC. 2014. Cardiovascular diseases: oxidative damage and antioxidant protection. Eur Rev Med Pharmacol Sci, 18: 3091-3096.