Antioxidant, cytotoxic and DNA protective properties of Achillea eriophora DC. and Achillea biebersteinii Afan. extracts: A comparative study

Document Type : Original Research Article

Authors

1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

2 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract


Objective: Achillea is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for Achillea pharmacological effects. We aimed to determine phenol and flavonoid contents, besides antioxidant activities of different extracts from Achillea eriophoraa (A. eriophora) DC. and Achillea biebersteinii (A. biebersteinii) Afan. (endemic species in Iran) and to investigate their effects on human cells.

Materials and Methods: Achillea extracts, were prepared by maceration and shaking methods, from different parts (aerial parts, stem, leaves and inflorescence) of two species using methanol and ethanol as solvents. Total phenol and flavonoid contents were measured by spectrophotometry, and antioxidant activities of the extracts were determined by DPPH radical scavenging, BCB and TBARS assays. Cytotoxicity and antioxidant activities of the extracts were investigated in Human Foreskin Fibroblast (HFF3) cells using MTT, comet and H2O2 assays.

Results: Methanol extracts of A. biebersteinii prepared from leaves and inflorescence by maceration method exhibited maximum phenol (1657.58 ± 36.45 mg GAE/100 g DW) and flavonoid (264.00 ± 62.16 mg QUE/100 g DW) contents. Leaf methanol extract showed significantly higher antioxidant activity (0.0276 ± 0.003, 0.16 ± 0.016 and 13.96 ± 0.26 mg/ml for DPPH, BCB and TBARS IC50s, respectively) than those of the other extracts. Leaf extract of A. biebersteinii was not cytotoxic even at the highest examined dose (512 µg/ml) and inhibited cell toxicity induced by H2O2 (98% viability for the cells pretreated with plant extract in the presence of H2O2). Comet assay also confirmed high DNA protective activity of leaf extracts.

Conclusion: Achillea extracts possess remarkable antioxidant activity, and could be good natural alternatives to synthetic antioxidants in pharmaceutical and food industries.

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Main Subjects


Adetutu A, Morgan WA, Corcoran O. 2011. Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. J Ethnopharmacol, 133: 116-119.
Afsharypuor S, Asgary S, Lockwood G. 1996. Constituents of the essential oil of Achillea wilhelmsii from Iran. Planta Med, 62: 77-78.
Amiri MS and Joharchi MR. 2013. Ethnobotanical investigation of traditional medicinal plants commercialized in the markets of Mashhad, Iran. Avicenna J Phytomed, 3: 254-271.
Annan K and Houghton PJ. 2008. Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana. J Ethnopharmacol, 119: 141-144.
Asgary S, Naderi G, Sarrafzadegan N, Mohammadifard N, Mostafavi S, Vakili R. 2000. Antihypertensive and antihyperlipidemic effects of Achillea wilhelmsii. Drugs Exp Clin Res, 26: 89-94.
Bashi DS, Fazly Bazzaz BS, Sahebkar A, Karimkhani MM, Ahmadi A. 2012.Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharm Biol, 50: 1168-1176.
Bazzaz BSF, Khayat MH, Emami SA, Asili J, Sahebkar A, Neishabory EJ. 2011. Antioxidant and antimicrobial activity of methanol, dichloromethane, and ethyl acetate extracts of Scutellaria litwinowii. ScienceAsia, 37: 327-334.
Behravan J, Mosafa F, Soudmand N, Taghiabadi E, Razavi BM, Karimi G. 2011. Protective effects of aqueous and ethanolic extracts of Portula caoleracea L. aerial parts on H2O2- induced DNA damage in lymphocytes by comet assay. J Acupunct Meridian Stud, 4:193-197.
Benetis R, Radušienė J, Jakštas V, Janulis V, Malinauskas F. 2008. Development of an RP‐HPLC method for the analysis of phenolic compounds in Achillea millefolium L. J Liq Chromatogr Rel Technol, 31: 596-610.
Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sökmen A, Akpulat HA. 2003. Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. Millefolium Afan. (Asteraceae). J Ethnopharmacol, 87: 215-220.
Ćetković GS, Čanadanović-Brunet JM, Djilas SM, Tumbas VT, Markov SL, Cvetković DD. 2007. Antioxidant potential, lipid peroxidation inhibition and antimicrobial activities of Satureja montana L. subsp. kitaibelii extracts. International Journal of Molecular Sciences, 8: 1013-1027.
Chandler R, Hooper S, Harvey MJ. 1982. Ethnobotany and phytochemistry of yarrow, Achillea millefolium, Compositae. Econ Bot, 36: 203-223.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal, 10: 178-182.
Chevallier A. 2000. Encyclopedia of herbal medicine: Dk Pub.
Chiarugi P and Fiaschi T. 2007.Redox signalling in anchorage-dependent cell growth. Cell Signal, 19: 672-682.
Conforti F, Loizzo MR, Statti GA, Menichini F. 2005.Comparative radical scavenging and antidiabetic activities of methanolic extract and fractions from Achillea ligustica ALL. Biol Pharm Bull, 28: 1791-1794.
Cook N and Samman S. 1996. Flavonoids chemistry, metabolism, cardioprotective effects, and dietary sources. J N B, 7: 66-76.
Dapkevicius A, Venskutonis R, van Beek TA, Linssen JP. 1998. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric, 77: 140-146.
Deba F, Xuan TD, Yasuda M, Tawata S. 2008. Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidenspilosa Linn. var. Radiata. Food control, 19: 346-352.
Dokhani S, Cottrell T, Khajeddin J, Mazza G. 2005. Analysis of aroma and phenolic components of selected Achillea species. Plant Foods Hum Nutr, 60: 55-62.
Evans WC. 2009. Trease and Evan's pharmacognosy: Elsevier Health Sciences.
Falk A, Smolenski S, Bauer L, Bell C. 1975. Isolation and identification of three new flavones from Achillea millefolium L. J Pharm Sci, 64: 1838-1842.
Ghorbani A. 2005. Studies on pharmaceutical ethnobotany in the region of Turkmen Sahra, north of Iran: (Part 1): General results. J Ethnopharmacol, 102: 58-68.
Gião MS, Leitão I, Pereira A, Borges AB, Guedes CJ, Fernandes JC, Belo L, Santos-Silva A, Hogg TA, Pintado ME. 2010. Plant aqueous extracts: Antioxidant capacity via haemolysis and bacteriophage P22 protection. Food Control, 21: 633-638.
Giorgi A, Madeo M, Speranza G, Cocucci M. 2010. Influence of environmental factors on composition of phenolic antioxidants of Achillea collina Becker ex Rchb. Nat Prod Res, 24: 1546-1559.
Giorgi A, Mingozzi M, Madeo M, Speranza G, Cocucci M. 2009. Effect of nitrogen starvation on the phenolic metabolism and antioxidant properties of yarrow (Achillea collina Becker ex Rchb.). Food Chem, 114: 204-211.
Goldberg AS, Mueller EC, Eigen E, Desalva SJ. 1969. Isolation of the anti‐inflammatory principles from Achillea millefolium (compositae). J Pharm Sci, 58: 938-941.
Haase G and Dunkley W. 1969. Ascorbic acid and copper in linoleate oxidation. II. Ascorbic acid and copper as oxidation catalysts. J Lipid Res, 10: 561-567.
Harborne JB and Williams CA. 2000. Advances in flavonoid research since 1992. Phytochemistry, 55: 481-504.
Hassan AM, Mohamed SR, El-Nekeety AA, Hassan NS, Abdel-Wahhab MA. 2010. Aquilegia vulgaris L. extract counteracts oxidative stress and cytotoxicity of fumonisin in rats. Toxicon, 56: 8-18.
Hsouna AB, Trigui M, Culioli G, Blache Y, Jaoua S. 2011. Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity. Food Chem, 125: 193-200.
Jacob RA. 1995. The integrated antioxidant system. Nutrition Research, 15: 755-766.
Konyalioglu S and Karamenderes C. 2005.The protective effects of Achillea L. species native in Turkey against H2O2-induced oxidative damage in human erythrocytes and leucocytes. J Ethnopharmacol, 102: 221-227.
Kumar S and Gupta S. 2011.Thymosin beta 4 prevents oxidative stress by targeting antioxidant and anti-apoptotic genes in cardiac fibroblasts. PLoS One, 6: e26912.
Kumpulainen JT and Salonen JT. 1999. Natural antioxidants and anticarcinogens in nutrition, health and disease: proceedings of the Second International Conference on Antioxidants and Anticarcinogens in Nutrition: Royal Society of Chemistry.
Lampronti I, Saab AM, Gambari R. 2006. Antiproliferative activity of essential oils derived from plants belonging to the Magnoliophyta division. Int J Oncol, 29: 989.
Lin K, Yang Y, Yang C, Huang M, Lo H, Liu K, Lin H, Chao P, 2013. Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes. BMC Research Notes, 6:490-500.
Mirheidari H. 1998. Application of plants in prevention and treatment of diseases: Office of Islamic culture pub.
Moeina S and Moeinb M. 2010. Antioxidant activities and phenolic content of Juniperus excels extract. Iranian Journal of Pharmaceutical Sciences, 6: 133-140.
Orhan I and Üstün O. 2011.Determination of total phenol content, antioxidant activity and acetylcholinesterase inhibition in selected mushrooms from Turkey. Journal of Food Composition and Analysis, 24: 386-390.
Özgen U, Mavi A, Terzi Z, Coflkun M. 2004. Antioxidant activities and total phenolic compounds amount of some Asteraceae species. Turkish J Pharm sci, 3: 203-216.
Pirbalouti A and Golparvar A. 2007. Evaluation of ethnobotany in the region of Chaharmahal & Bakhtyari, West Central Iran. Paper presented at The Abstract book of 48th annual meeting of the Society for Economic Botany; June 4-7, 2007, Chicago, Ill., USA.
Ramos S. 2008. Cancer chemoprevention and chemotherapy: dietary polyphenols and signalling pathways. Mol Nutr Food Res, 52: 507-526.
Rassouli FB, Matin MM, Iranshahi M, Bahrami AR, Behravan J, Mollazadeh S, Neshati V, Kalalinia F. 2011. Investigating the enhancement of cisplatin cytotoxicity on 5637 cells by combination with mogoltacin. Toxicol In Vitro, 25: 469-474.
Rezaeipoor R, Saeidnia S, Kamalinejad M. 1999. Immunosuppressive activity of Achillea talagonica on humoral immune responses in experimental animals. J Ethnopharmacol, 65: 273-276.
Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res, 22: 375-383.
Saeidnia S, Gohari A, Mokhber-Dezfuli N, Kiuchi F. 2011. A review on phytochemistry and medicinal properties of the genus Achillea. DARU: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 19: 173.
Sánchez-Moreno C, A Larrauri J, Saura-Calixto F. 1999. Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res Int, 32: 407-412.
Shahidi F, Janitha P, Wanasundara P. 1992. Phenolic antioxidants. Crit Rev Food SciNutr, 32: 67-103.
Sokmen M and Özbek T. 2006. Biological activities of the essential oil and methanol extract of Achillea biebersteinii Afan. (Asteraceae). Turk J Biol, 30: 65-73.
Štajner D, Milić N, Mimica‐Dukić N, Lazić B, Igić R. 1998. Antioxidant abilities of cultivated and wild species of garlic. Phytother Res, 12: S13-S14.
Stojanović G, Radulović N, Hashimoto T, Palić R. 2005. In vitro antimicrobial activity of extracts of four Achillea species: The composition of Achillea clavennae L. (Asteraceae) extract. J Ethnopharmacol, 101: 185-190.
Swain T and Hillis W. 1959.The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J Sci Food Agric, 10: 63-68.
Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. 2007. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem, 104: 1372-1378.
Weber KC, Honório KM, Bruni AT, da Silva AB. 2006. The use of classification methods for modeling the antioxidant activity of flavonoid compounds. J Mol Model, 12: 915-920.
Yang H, Dong Y, Du H, Shi H, Peng Y, Li X. 2011. Antioxidant compounds from propolis collected in Anhui, China. Molecules, 16: 3444-3455.
Yazdanparast R, Ardestani A, Jamshidi S. 2007. Experimental diabetes treated with Achillea santolina: Effect on pancreatic oxidative parameters. J Ethnopharmacol, 112: 13-18.
Yildirim A, Mavi A, Kara AA. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem, 49: 4083-4089.
Yoo KM, Lee CH, Lee H, Moon B, Lee CY. 2008. Relative antioxidant and cytoprotective activities of common herbs. Food Chem, 106: 929-936.
Yoo KM, Al-Farsi M, Lee H, Yoon H, Lee CY. 2010. Antiproliferative effects of cherry juice and wine in Chinese hamster lung fibroblast cells and their phenolic constituents and antioxidant activities. Food Chem, 123: 734-740.
Yu L, Zhao M, Cui C, Yang B, Jiang Y, Zhao Q. 2008. Antioxidant, immunomodulatory and anti-breast cancer activities of phenolic extract from pine (Pinus massoniana Lamb) bark. Innovative Food Science & Emerging Technologies, 9: 122-128.
Zargari A. 1993. Medicinal Plants. Tehran. Iran: University of Tehran Press.