TY - JOUR ID - 13681 TI - Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Ranjbar, Nasrin AU - Saravani, Ramin AU - Faezizadeh, Zohreh AD - Student Scientific Research Center, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. AD - Cellular and Molecular Research Center, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. AD - Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran Y1 - 2020 PY - 2020 VL - 10 IS - 2 SP - 161 EP - 169 KW - Toll-Like Receptor KW - TLR8 KW - Gene expression KW - Silymarin KW - Apoptosis KW - Ramos cells DO - 10.22038/ajp.2019.13681 N2 - Objective: Silymarin is a herbal extract containing flavonolignans, and it has inhibitory effects against the growth of different cancer cell lines by inducing apoptosis. Toll-like receptors are suggested as a novel and attractive target to treat cancer. The current study aimed at examining the mechanism of silymarin-induced apoptosis in Ramos cells and investigating its effects on TLR8 expression. Materials and Methods: The half maximal inhibitory concentration (IC50) of silymarin in Ramos cells was determined via MTT viability test while the type of cell death was tested by annexin V/propidium iodide (PI) double staining method. The activity of caspase-3 and expression of TLR8 were measured in a time-dependent manner (in IC50) by colorimetric assay and real-time polymerase chain reaction (RT-PCR), respectively. Results: The results of MTT showed that IC50 of silymarin in Ramos cells was 100 μg/ml after 48 hr treatment (p<0.01). Flow cytometry by annexin V/PI, showed that silymarin induced early/late apoptosis in this cell line (p Conclusion: The results indicated a new mechanism in the anticancer activity ofToll-like receptor (TLR) signaling after silymarin treatment in Ramos cancer cell line. This plant could be used to develop anticancer agents inhibiting TLRs. UR - https://ajp.mums.ac.ir/article_13681.html L1 - https://ajp.mums.ac.ir/article_13681_a72f63212f48d50e9d89e8c71cc24b7b.pdf ER -