The field efficacy of Nigella sativa and Berberis vulgaris methanolic extracts against Haemoproteus columbae

Document Type: Short communication

Authors

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Objective: The methanolic extracts of Nigella sativaL. seeds (MENS) and Berberis vulgaris L.(MEBV) were investigated for treatment of Haemoproteus columbae-infected pigeons (Columba livia domestica).
Materials and Methods: One hundred twenty naturally-infected pigeons were randomly divided into four groups of thirty each. Two groups were treated separately with the extracts, while the positive and negative control groups were given buparvaquone (Butalex®) and distilled water, respectively. The parasitaemia rate was calculated in all groups before and after the experiment at four-day intervals for16 days.
Results: The results showed a high therapeutic effect for MENS with a progressive decrease in average parasitaemia rate from 18.17% before treatment to 0.73% at the end of treatment (p<0.05), while Butalex® was able to suppress the parasitemia rate from 18.90% before treatment to 0.23% at the end of experiment (p<0.05). However, no significant changes in parasitemia rate were evident in groups treated with MEBV (p>0.05).
Conclusion: Methanolic extracts of N. sativa showed therapeutic effects against H. columbae and may be regarded as a suitable choice for further studies to develop new drugs against blood parasites, in both animals and human beings.

Keywords

Main Subjects


Ahmad  A, Husain  A, Mujeeb M, Khan SA, Najmi  AK, Siddique NA, Damanhouri ZA, Anwar F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed, 3: 337-352.

Al-Naggar T, Gomez-Serranillos M, Carretero M, Villar A. 2003. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol, 88: 63-68.

Ashford Rw. 1971. Blood parasites and migratory fat at Lake Chad. 113, pp.100-101, Ibis, John Wiley & Sons.

Atkinson CT, Dusek RJ, Woods KL, Iko WM. 2000. Pathogenicity of avian malaria in experimentally-infected Hawaii Amakihi. J Wildl Dis, 36: 197-201.

Bishopp F. 1929. The pigeon fly-an important pest of pigeons in the United States. J Econ Entomol, 22: 974-980.

Cardona CJ, Ihejirika A, McClellan L. 2002. Haemoproteus lophortyx infection in bobwhite quail. Avian Dis, 46: 249-255.

Cheesman S. 2000. The topoisomerases of protozoan parasites. Parasitol Today, 16: 277-281.

El-Metenawy T. 1999. Therapeutic effects of some antihaematozoal drugs against Haemoproteus columbae in domestic pigeons. DtschTierarztlWochenschr, 106: 72-72.

El-Shabrawy O, Nada S. 1996. Biological evaluation of multicomponent tea used as hypoglycemic in rats. Fitoterapia, 67: 99-102.

El Zalabani SM, El-Askary HI, Mousa OM, Issa MY, Zaitoun AA, Abdel-Sattar E. 2012. Acaricidal activity of Swieteniam ahogani and Swieteniam acrophylla ethanolic extracts against Varroa destructor in honeybee colonies. Exp Parasitol, 130: 166-170.

Fata A, Rakhshandeh H, Berenji F, Jalilianfard A. 2006. Treatment of Cutaneous Leishmaniasis in murine model by Alcoholic Extract of Berberis vulgaris. Iran J Parasitol, 1: 39-42.

Garvin MC, Homer BL, Greiner EC. 2003. Pathogenicity of Haemoproteus danilewskyi, Kruse, 1890, in blue jays (Cyanocitta cristata). J Wildl Dis, 39: 161-169.

Gessler M, Nkunya MH, Mwasumbi LB, Heinrich M, Tanner M. 1994. Screening Tanzanian medicinal plants for antimalarial activity. Acta Trop, 56: 65-77.

Ishtiaq F, Gering E, Rappole JH, Rahmani AR, Jhala YV, Dove CJ, Milensky C, Olson SL, Peirce MA, Fleischer RC. 2007. Prevalence and diversity of avian hematozoan parasites in Asia: a regional survey. J Wildl Dis, 43: 382-398.

Khan N, Sultana S. 2005. Inhibition of two stage renal carcinogenesis, oxidative damage and hyper proliferative response by Nigella sativa.Eur J Cancer Prev, 14: 159-168.

Knowles S, Palinauskas V, Sheldon B. 2010. Chronic malaria infections increase family inequalities and reduce parental fitness: experimental evidence from a wild bird population. J Evol Biol, 23: 557-569.

Mahmoud M, El-Abhar H, Saleh S. 2002. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol, 79: 1-11.

Mahmoudvand H, Sharififar F, Rahmat MS, Tavakoli R, Dezaki ES, Jahanbakhsh S, Sharifi I. 2014. Evaluation of anti-leishmanial activity and cytotoxicity of the extracts of Berberis vulgaris and Nigella sativa against Leishmania tropica. J Vector Borne Dis, 51: 294.

Majdalawieh AF, Hmaidan R, Carr RI. 2010. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol, 131: 268-275.

Manwell RD, Loeffler CA. 1961. Glucose consumption by Haemoproteus columbae. J Parasitol, 47: 285-290.

Martinsen ES, Perkins SL, Schall JJ. 2008. A three-genome phylogeny of malaria parasites (Plasmodium and closely related genera): evolution of life-history traits and host switches. Mol Phylogenet Evol, 47: 261-273.

Moazeni M, Nazer A. 2010. In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World J Surg, 34: 2677-2681.

Møller AP, Nielsen JT. 2007. Malaria and risk of predation: a comparative study of birds. Ecology, 88: 871-881.

Muregi F, Chhabra S, Njagi E, Lang'at-Thoruwa C, Njue W, Orago A, Omar S, Ndiege I. 2003. In vitro antiplasmodial activity of some plants used in Kisii, Kenya against malaria and their chloroquine potentiation effects. J Ethnopharmacol, 84: 235-239.

Nasir A, Avais M, Khan M, Khan J, Hameed S, Reichel M. 2013. Treating Cryptosporidium parvum infection in calves. ‎J Parasitol, 99: 715-717.

O'roke EC. 1930. The Morphology, Transmission, and Life-history of Haemoproteus lophortyxO'Roke, a Blood Parasite of the California Valley Quail, Univ California Pub Zool, 36: 1-50.

Okeola VO, Adaramoye OA, Nneji CM, Falade CO, Farombi EO, Ademowo OG. 2011. Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis. Parasitol Res, 108: 1507-1512.

Rahman NNNA, Furuta T, Takane K, Mohd MA. 1999. Antimalarial activity of extracts of Malaysian medicinal plants. J Ethnopharmacol, 64: 249-254.

Razavi SM, Asadpour M, Jafari A, Malekpour SH. 2015. The field efficacy of Lepidium latifolium and Zataria multiflora methanolic extracts against Varroa destructor. Parasitol Res, 114: 4233-4238.

Ricklefs RE, Fallon SM, Bermingham E. 2004. Evolutionary relationships, cospeciation, and host switching in avian malaria parasites. Syst Biol, 53: 111-119.

Ricklefs RE, Swanson BL, Fallon SM, MartÍnez-AbraÍn A, Scheuerlein A, Gray J, Latta SC. 2005. Community relationships of avian malaria parasites in southern Missouri. Ecol Monogr, 75: 543-559.

Rodrigues JR, Gamboa ND. 2009. Effect of dequalinium on the oxidative stress in Plasmodium berghei-infected erythrocytes. Parasitol Res, 104: 1491-1496.

Salehabadi A, Karamian M, Farzad MH, Namaei MH. 2014. Effect of root bark extract of Berberis vulgaris L. on Leishmania major on BALB/c mice. Parasitol Res, 113: 953-957.

Salem ML, Hossain MS. 2000. Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection. Int J Immunopharmacol, 22: 729-740.

Slater LB. 2005. Malarial birds: Modeling infectious human disease in animals. Bull Hist Med, 79: 261-294.

Tomás G, Merino S, Moreno J, Morales J, Martinez‐De La Puente J. 2007. Impact of blood parasites on immunoglobulin level and parental effort: a medication field experiment on a wild passerine. FunctEcol, 21: 125-133.

Valkiunas G. 2004. Avian malaria parasites and other haemosporidia. pp. 12-16, CRC press, London, Taylor & Francis.

Valkiunas G, Zickus T, Shapoval AP, Iezhova TA. 2006. Effect of Haemoproteus belopolskyi (Haemosporida: Haemoproteidae) on body mass of the blackcap Sylvia atricapilla. J Parasitol, 92: 1123-1125.

Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. 2002. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine, 9: 69-74.