A simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples

Document Type: Original Research Article

Authors

1 Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Science, Tehran, Iran

2 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

4 Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples.
Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55, v/v, pH 3.5), C18 column (150 mm×4.6 mm×5 µm particle size) and a flow rate of 1 ml/min in ambient condition and total retention time of 17 min. The volume of injection was set at 20 µl and detection was recorded at 425 nm. The robustness of the method was examined by changing the mobile phase composition, mobile phase pH, and flow rate.
Results: The method was validated with respect to precision, accuracy and linearity in a concentration range of 2-100 µg/ml. The limit of detection (LOD) and limit of quantification (LOQ) were 0.25 and 0.5 µg/ml, respectively. The percentage of recovery was 98.9 to 100.5 with relative standard deviation (RSD) < 0.638%.
Conclusion: The method was found to be simple, sensitive and rapid for determination of curcumin in pharmaceutical samples and had enough sensitivity to detect degradation product of curcumin produced under photolysis and hydrolysis stress condition.

Keywords

Main Subjects


Aggarwal BA, Kumar A, Aggarwal MS, Shishodia S. 2005. Curcumin derived from turmeric (Curcuma longa): a spice for all seasons. In: Phytopharmaceuticals in Cancer Chemoprevention. CRC Press LLC, pp. 349–387

Aggarwal BB, Kumar A, Bharti AC. 2003. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res, 23:363–398

Aggarwal BB and Harikumar KB. 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol, 41: 40–59

Ammon HP, Wahl MA. 1991. Pharmacology of Curcuma longa. Planta Med, 57:1–7

Ankola DD, Kumar. MNV. 2009. Nanoparticle encapsulation improves oral bioavailability of curcumin by at least 9-fold when compared to curcumin administered with piperine as absorption enhancer. Eur J Pharm Sci, 37:223-230

Anand P, Nair BH, Aggarwal BB. 2010. Design of curcumin-loaded PLGA nanoparticles formulation with enhanced cellular uptake, and increased bioactivity in vitro and superior bioavailability in vivo. J Biochem, 79: 330-338

Anand P, Sundaram C, Jhurani S, Kunnumakkara AB, Aggarwal BB. 2008. Curcumin and cancer: an “old-age” disease with an “age-old”. Solution, Cancer Lett, 267:133–164

Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. 2004. Turmeric and curcumin: biological actions and medicinal applications. Curr Sci, 87: 44–53

Chattopadhyay I, Biswas K, Bandyopadhyay U, Zhou RK, Beevers H, Huang S. 2011. The targets of curcumin. Curr Drug Targets, 12:332-347.

Dandekar PP, Patravale VB. 2009. Development and validation of a
stability-indicating LC method for curcumin. Chromatographia, 69:871-877

Jayaprakasha GK, Jena BS, Negi PS, Sakariah KK. 2002, Evaluation of antioxidant activities and antimutagenicity of turmeric oil: a byproduct from curcumin production, Z Naturforsch C, 57:828-835.

Kim HT, Jiang HH. 2011. Preparation and characterization of water-soluble albumin-bound curcumin nanoparticles with improved antitumor activity. Int J Pharm, 403: 285-291

Koranya MA,  Haggaga RS,  Ragaba MA, Elmallahb OA. 2013. A validated stability-indicating HPLC method for simultaneous determination of Silymarin and Curcumin in various dosage forms. J Arab Chem, In press.

Rahimi HR, Mohammadpour AH, Dastani M, Jafari MR, AbnousK, Mobarhan MG, Kazemi Oskuee R. 2016. The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial. Avicenna J Phytomed, 6: 567-577.

Rahimi HR, Nedaeinia R, Sepehri Shamloo A, Nikdoust Sh, Kazemi Oskuee R. 2016. Novel delivery for natural products: Nano-curcumin formulations. Avicenna J Phytomed, 6: 383-398.

Rouseff RL. 1988. High performance liquid chromatographic separation and spectral characterization of the pigments in turmeric and annatto. J Food Sci, 53:1823-1826

Tonnesen HH, Karlsen J. 1985. Studies on curcumin and curcuminoids. VI. Kinetics of curcumin degradation in aqueous solution. Z. Lebensm. Unters. Forsch, 180: 402–404

Tonnesen HH, Karlsen J. 1985a. Studies of curcumin and curcuminoids. V. Alkaline degradation of curcumin. Z. Lebensm. Unters. Forsch, 180: 132-134

Tonnesen HH, Karlsen J, van Henegouwen GB. 1986. Studies on curcumin and curcuminoids. VIII. Photochemical stability of curcumin. Z Lebensm Unters Forsch, 183:116-122.

Wang YJ, Pan MH, Cheng AL, Lin LI, Ho YS, Hsieh CY, Lin JK. 1997. Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed, 15:1867–1876

Wichitnithad W, Jongaroonngamsang N, Pummangura S, Rojsitthisak P. 2009. A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts. Phytochem Anal, 20:314-319.