1Department of Biology, Faculty of Science, Islamic Azad University, Mashhad Branch, I. R. Iran
2Applied Physiology Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
3School of Public Health, Tehran University of Medical Sciences and Researches on Industrial Biotechnology, Tehran, I. R. Iran
4Islamic Azad University, Mashhad Branch, and Zakaria Reserch Center, Mashhad, I. R. Iran
5Faculty of Medicine, NHLI, Imperial College, London, UK
Objective: The effects of natural adjuvants were examined on total and differential WBC counts in lung lavage of sensitized guinea pigs. Materials and Methods: In three sensitized groups of guinea pigs including: untreated sensitized animals (S), sensitized animals treated with adjuvant G2 (S+G2) and G2F (S+G2F) as well as non-sensitized group (C) (n=6 for each group), total and differential WBC counts of lung lavage were examined. Sensitization of animals was achieved by injection and inhalation of ovalbumin (OA). Results: The results showed increased total WBC, eosinophil, neutrophil, and basophil counts, and decreased lymphocytes in lung lavage of sensitized animals compared with the control group (pConclusion: These results indicate important preventive effects of two natural adjuvants, especially G2, on lung inflammation of sensitized guinea pigs.
Boskabady MH, Javan H, Sajadi M, Rakhshandah H. 2007. The possible prophylactic effect of Nigella sativa seed extract in asthmatic patients. Fundament Clin Pharmacol, 21: 559-566.
Boskabady MH, Kiani S. 2007. The effect of expouser of guinea pigs to cigarette smoke and their sensitization with ovalbumin in tracheal responsiveness to histamine and hisatamine (H1) receptor blockade by chlorpheniramine. Pathophysiology, 14: 97-104.
Boskabady MH, Ramazani M. 2001. Relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol, 74: 83-88.
Boskabady MH, Ziaei T. 2003. Effect of ascorbic acid on airway responsiveness in ovalbumin sensitized guinea pigs. Respirology, 8: 473-478.
Boskabady MH, Kiani S, Aslani MR. 2006. Tracheal responsiveness to both isoprenaline and beta2- adrenoreceptor blockade by propranolol in cigarette smoke exposed and sensitized guinea pigs. Respirology, 11: 572-578.
Busse W, Banks-Schlegel SP, Larson GL. 1995. Childhood versus adult-onset asthma. Am J Respir Crit Care Med, 151: 1635-1639.
Cho YS, Lee J, Lee TH. 2004. Alph-Lipoic acid inhibits airway inflammation and hyperresponsiveness in a mouse model of asthma. J Allergy Clin Immunol, 114: 429-435.
Kimura J, Nariuchi H, Watanabe T, et al. 1978a. Studies on the adjuvant effect of water-in-oil-in-water (w/o/w) emulsion of sesame oil. Enhanced and persistent antibody formation by antigen incorporated into the water-in-oil-in-water emulsion. Jpn J Exp Med, 48: 149-54.
Kimura J, Nariuchi H, Watanabe T, et al. 1978b. Studies on the adjuvant effect of water-in-oil-in-water (w/o/w) emulsion of sesame oil. 2. Mode of action of the w/o/w emulsion. Jpn J Exp Med, 48: 203-209.
McCaig D, De Jonckheere S. 1993. Effect of two Ca2+ modulator in normal and albumin sensitized guinea-pig trachea. Eur J Pharmacol, 249: 53-63.
Mohaghegh Hazrati S, Aghazadeh J, Mohtarami F, et al. 2006. Immunotherapy of Prolactinoma with a T Helper 1 Activator Adjuvant and Autoantigens: A Case Report. Neuroimmunomodulat, 13: 205-208.
Mohaghegh Hazrati S, Khanbaba CR, Mazaheri B, et al. 1999. A novel an invasive metastatic Adenocarcinoma of breast cancer (HAZ-1) in small with laboratory mice. 14th Iran Con Physiol Pharmacol, p262.
Mohaghegh HS, Nasiri Khalaji S, Mohtarami F. et al. 2004. G2, PC and G2F in allergic asthma. Iran J Ped, 13: 30.
Neamati A, Boskabady MH, Tavakol Afshari J, et al. 2009. The effect of the natural adjuvants on tracheal responsiveness, and cell count of lung lavage in sensitized guinea-pig. Respirology, 14: 877-884.
Niwa Y, Kanoh T, Kasama T, et al. 1988. Activation of antioxidant activity in natural medicinal products by heating, brewing and lipophilization. A new drug delivery system. Drugs Exp Clin Res, 14: 361-372.
Padrid P, Snook S, Finucane T, et al. 1995. Persistent airway hyperresponsiveness and histologic alteration after chronic antigen challenge in cats. Am J Respir Crit Care Med, 151: 184-193.
Pope BL, Chourmouzis E, Sigindere J, et al. 1992. In vivo enhancement of murine natural killer cell activity by 7-allyl-8-oxoguanosine loxoribine. Int J Immunopharmacol, 14: 1375-1382.
Romieu I, Sienra-Monge JJ, Ramirez-Aguilar M, et al. 2002. Antioxidants supplementation and lung function among asthmatic children exposed to high levels of air pollutants. Am J Respir Crit Care Med, 166: 703-709.
Sackesen C, Ercan H, Dizdar E, et al. 2008. A comprehensive evaluation of the enzymatic and nonenzymatic antioxidant systems in childhood asthma. J Allergy Clin Immunol, 122: 78-85.
Trenga CA, Koenig JQ, Williams PV. 2001. Dietary antioxidants and ozone-induced bronchial hyperresponsiveness in adults with asthma. Arch Environ Health, 56: 242-249.