Protective effect of Tribulus terrestris fruit extract on cerulein-induced acute pancreatitis in mice

Document Type: Original Research Article

Authors

1 School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Objective: Antioxidant, anti-inflammatory, analgesic and antimicrobial activities of Tribulus terrestris (T. terrestris) could be helpful in the treatment of acute pancreatitis; thus, this study was designed to investigate the effects of T. terrestris on cerulein-induced acute pancreatitis in mice.
Materials and Methods: Three doses (100, 200 and 400 mg/kg) of T. terrestris hydro-alcoholic extract were administered both orally (60 minutes before pancreatitis induction, p.o.) and intra-peritoneally (30 minutes before pancreatitis induction, i.p.) to different groups of mice (n=6). Pancreatitis was induced by five injections (i.p.) of cerulein 50μg/kg body weight with 1 hr intervals. Animals were euthanized 5 hr after the last injection of cerulein and tissue injures were assessed biochemically and pathologically.
Results: T. terrestris extract 200 and 400mg/kg (p.o.) and T. terrestris extract 400 mg/kg (i.p.) reduced pancreatic tissue myeloperoxidase (MPO) activity and serum amylase and lipase levels and alleviated histological parameters.
Conclusion: These data suggest that T. terrestris hydro-alcoholic extract was effective in protecting against experimental acute pancreatitis and possibly the efficacy depends on dose and route of administration.

Keywords

Main Subjects


Abed A, Minaiyan M, Ghannadi A, Mahzouni P, Babavalian MR. 2012. Effect of Echium amoenum Fisch. et Mey a traditional Iranian herbal remedy in an experimental model of acute pancreatitis. ISRN Gastroenterol, 2012:141548.

Badalov N, Baradarian R, Iswara K, Li J, Steinberg W, Tenner S. 2007. Drug-induced acute pancreatitis: an evidence-based review. Clin Gastroenterol Hepatol, 5:648-661.

Bedir E, Khan I, Walker L . 2002. Biologically active steroidal glycosides from Tribulus terrestris. Pharmazie, 57:491-493.

Beger H, Rau B, Mayer J, Pralle U. 1997. Natural course of acute pancreatitis. World J Gastrointest Surg, 21:130-135

Bradley PP, Christensen RD, Rothstein G. 1982. Cellular and extracellular myeloperoxidase in pyogenic inflammation. Blood, 60:618-622.

Chhatre S, Nesari T, Somani G, Kanchan D, Sathaye S. 2014. Phytopharmacological overview of Tribulus terrestris. Pharmacogn Rev, 8:45.

Chu S, Qu W, Pang X, Sun B, Huang X. 2003. Effect of saponin from Tribulus terrestris on hyperlipidemia. Zhong Yao Cai, 26:341-344.

Cushnie TT, Lamb AJ. 2005. Antimicrobial activity of flavonoids. Int J Antimicrob Agents, 26:343-356.

Dembiński A, Warzecha Z, Ceranowicz P, et al. 2008. Dual, time-dependent deleterious and protective effect of anandamide on the course of cerulein-induced acute pancreatitis. Eur J Pharmacol, 591:284-292.

Genovese T, Mazzon E, Di Paola R, et al. 2006. Hypericum perforatum attenuates the development of cerulein-induced acute pancreatitis in mice. Shock , 25:161-167.

Graham DY . 2000. NSAID ulcers: prevalence and prevention. Mod Rheumatol, 10:2-7.

Hajhashemi V, Ghannadi A, Hajiloo M. 2010. Analgesic and anti-inflammatory effects of Rosa damascena hydroalcoholic extract and its essential oil in animal models. Iran J Pharm Res, 9:163

Hamilton M, Sheehan M, De Belleroche J, Herberg L. 1984. The cholecystokinin analogue, caerulein, does not modulate dopamine release or dopamine-induced locomotor activity in the nucleus accumbens of rat. Neurosci Lett, 44:77-82.

Heidari MR, Mehrabani M, Pardakhty A, et al. 2007. The analgesic effect of Tribulus terrestrisextract and comparison of gastric ulcerogenicity of the extract with indomethacine in animal experiments. Ann N Y Acad Sci, 1095:418-27.

Hong CH, Hur SK, Oh O-J, Kim SS, Nam KA, Lee SK. 2002. Evaluation of natural products on inhibition of inducible cyclooxygenas (COX-2) and nitric oxide synthase (iNOS) in cultured mouse macrophage cells. J Ethnopharmacol, 83:153-159.

Kambhampati S, Park W, Habtezion A . 2014. Pharmacologic therapy for acute pancreatitis. World J Gastroenterol, 20:16868.

Katalinic V, Milos M, Kulisic T, Jukic M. 2006. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols.  Food Chem, 94:550-557.

Kaurich T. 2008. Drug-induced acute pancreatitis. Baylor University Medical Center, 21:77-89.

Kianbakht S, Jahaniani F. 2003. Evaluation of antibacterial activity of Tribulus terrestrisL. growing in Iran. Iran J Pharm, 2:22-24.

Kim H. 2008. Cerulein pancreatitis: oxidative stress, inflammation, and apoptosis. Gut Liver, 2:74-80.

Kim HJ, Kim JC, Min JS, et al . 2011. Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. J Ethnopharmacol, 136:197-203.

Kostova I, Dinchev D. 2005. Saponins in Tribulus terrestris–chemistry and bioactivity. Phytochem Rev, 4:111-137.

Lakhanpal P, Rai DK. 2007. Quercetin: a versatile flavonoid. Int J Med Up, 2:22-37.

Lankisch PG, Banks PA . 2013. Pancreatitis. In:Springer Science & Business Media, pp.279-286.

Michalski CW, Laukert T, Sauliunaite D, et al. 2007. Cannabinoids ameliorate pain and reduce disease pathology in cerulein-induced acute pancreatitis. Gastroenterol Rep, 132:1968-1978.

Minaiyan M, Ghannadi A-R, Mahzouni P, Abed A-R . 2012. Preventive effect of Cichorium intybus L. two extracts on cerulein-induced acute pancreatitis in mice. Int J Prev Med, 3(5):351.

Minaiyan M, Hajhashemi V, Rabbani M, Fattahian E, Mahzouni P. 2014a. Beneficial Effects of Maprotiline in a Murine Model of Colitis in Normal and Reserpinised Depressed Rats. Res Pharm Sci, 10:295-306.

Minaiyan M, Zolfaghari B, Taheri D, Gomarian M. 2014b. Preventive Effect of Three Pomegranate (Punica granatum L.) Seeds Fractions on Cerulein-Induced Acute Pancreatitis in Mice. Int J Prev Med, 5:394-404.

Mishra N, Biswal G, Chowdary K, Mishra G. 2013. Anti-arthritic activity of Tribulus terrestris studied in Freund's Adjuvant induced arthritic rats. J Adv Pharm Technol Res, 4:41-48.

Neychev VK, Mitev VI. 2005. The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. J Ethnopharmacol, 101:319-323.

Ojha S, Nandave M, Kumari S, Arya D. 2006. Antilipidperoxidative and free radical scavenging activity of Tribulus terrestris. Ind J Drug- 43(2):136.

Pooran N, Indaram A, Singh P, Bank S. 2003. Cytokines (IL-6, IL-8, TNF): early and reliable predictors of severe acute pancreatitis. Clin J Gastroenterol, 37:263-266.

Qiong W, Yiping W, Jinlin Y, Tao G, Zhen G, Pengcheng Z. 2005. Cochrane Database Syst Rev, 25:31-36.

Rajesh B, Fleming A, Ranvir R, Sundar R, Devada S. 2013. Effect of Tribulus terrestris against inflammatory bowel disease. Int J Vet Sci Med, 2:143-150.

Raraty MG, Connor S, Criddle DN, Sutton R, Neoptolemos JP. 2004. Acute pancreatitis and organ failure: pathophysiology, natural history, and management strategies. Curr Gastroenterol Rep, 6:99-103

Rasmussen H, Fonager K, Sørensen H, Pedersen L, Dahlerup JF, Steffensen F. 1999. Risk of acute pancreatitis in patients with chronic inflammatory bowel disease: a Danish 16-year nationwide follow-up study. Scand J Gastroenterol Suppl, 34:199-201.

Rogerson S, Riches CJ, Jennings C, Weatherby RP, Meir RA, Marshall-Gradisnik SM. 2007. The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. J Strength Cond Res 21:348-353.

Sakorafas GH, Tsiotou A . 2000. Etiology and pathogenesis of acute pancreatitis: current concepts. Clin J Gastroenterol, 30:343-356.

Shaheen G, Ahmad I, Akhter KUN, Ahmad M, Sultana S, Akram M. 2012. Monograph of Tribulus terrestris. J Med Plant Res, 6:641-644.

Shan B, Cai YZ, Sun M, Corke H. 2005. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J Agric Food Chem, 53:7749-7759.

Spanier BM, Dijkgraaf MG, Bruno MJ. 2008. Epidemiology, aetiology and outcome of acute and chronic pancreatitis: An update. Best Pract Res Clin Gastroenterol, 22:45-63.

Wang G-J, Gao C-F, Wei D, Wang C, Ding S-Q. 2009. Acute pancreatitis: etiology and common pathogenesis. World J Gastroenterol, 15:1427-1430.

Waterman PG, Mole S . 1994. Analysis of phenolic plant metabolites. Int J Lab Hematol, 1994:238.

Zheleva-Dimitrova D, Obreshkova D, Nedialkov P. 2012. Antioxidant activity of tribulus terrestris—a natural product in infertility therapy. Int J Pharm Pharm Sci, 4:508-511.

Zheng W, Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem, 49:5165-5170.