Potentiating effects of Perovskia abrotanoides on pentobarbital-induced sleep

Document Type: Original Research Article

Authors

1 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Biology, Payame Noor University, Tehran, Iran

Abstract


Objective: Sleeplessness is the most common sleep disorder. In this study the hypnotic effect of the hydro-alcoholic extract (HAE) of Perovskia abrotanoides and its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were studied in mice.

Materials and Methods: The test compounds were administered intraperitoneally to mice 30 min before the administration of sodium pentobarbital (30 mg/kg.). Moreover, the influence of flumazenil on the hypnotic effect of the extracts was evaluated. Besides, 30 min after administration of HAE, motor coordination (rota-rod test) was assessed. Additionally, LD50 for HAE was determined and the possible neurotoxicity of the extract was tested in neural PC12 cells.

Results: The HAE and NBF decreased the latency of sleep (p<0.05), and significantly increased the duration of sleep (p<0.05) induced by pentobarbital. These effects of P. abrotanoides were reversed by flumazenil. HAE did not affect the animals’ performance on the rota-rod test. The LD50 value for HAE was found to be 4.8 g/kg. HAE and its fractions did not show neurotoxic effects in cultured PC12 cell line.

Conclusion: The results showed that P. abrotanoides significantly potentiated pentobarbital hypnosis without toxic effect. Probably, its effects are related to its non-polar constituents.

Keywords

Main Subjects


Akhila JS, Shyamjith D, Alwar MC. 2007. Acute toxicity studies and determination of median lethal dose. Curr Sci, 93: 917-920.

Alnamer R, Alaoui KE, Bouidida H, Benjouad A, Cherrah Y. 2012. Sedative and hypnotic activities of themethanolic and aqueousextracts of Lavandula officinalis from Morocco. Adv Pharmacol Sci, 2012: 1-5.

Ballabh B, Chaurasia OP, Ahmed Z, Singh SB. 2008. Traditional medicinal plants of cold desert Ladakh used against kidney and urinary disorders. J Ethnopharmacol, 118: 331 – 339.

Bateson AN. 2012.  Further potential of the GABA receptor in the treatment of insomnia, Sleep Med, 7: S3–S9.

 Beikmohammadi M. 2012.The evaluation of medicinal properties of Perovskia abrotanoides Karel.  Middle East J Sci Res,  11: 189 – 193.

Cho SM, Shimizu M, Lee CJ, Han DS, Jung CK, Jo JH, Kim YM. 2010.  Hypnotic  effects  and binding  studies  for  GABA  (A)  and  5-HT  (2C) receptors  of  traditional  medicinal  plants  used  in Asia for insomnia. J Ethnopharmacol, 132: 225-232.

Esmaeilli S, Naghibi F, Mosaddegh M, Sahranavard S, Ghafari S, Abdullah N.R. Screening of antiplasmodial properties traditionally used among some Iranian plants. J Ethnopharmacol. 121: 400 – 404.

Ghorbani A, Rakhshandeh H, Asadpour E, Sadeghnia HR. 2012. Effects of Coriandrum sativum extracts on glucose/serum deprivation-induced neuronal cell death. Avicenna J Phytomed, 2 : 4-9.

Ghorbani A, Rakhshandeh H, Sadeghnia HR. 2013. Potentiating Effects of Lactuca sativa on Pentobarbital-Induced Sleep. Iran J Pharm Res, 12: 401-406.

Herrera-Ruiz M, Gutierrez C, Enrique J, Tortoriello J, Miron G. 2007. Central nervous system depressant activity of an ethyl acetate extract from Ipomoea stans roots. J Ethnopharmacol. 112: 243-247.

Hosseini A, Ghorbani A, Sadeghnia HR, Rajabian A, Rakhshandeh H. 2014. Potentiating effects of Lactuca serriola on pentobarbital-induced sleep. Res Opin Anim Vet Sci, 4: 601-607.

Hosseinzadeh H, Amel S. 2001. Antinociceptive effects of the aerial parts of Perovskia abrotanoides extracts in mice.  Iran Red Crescent Med J,  4: 15 – 27.

Hosseinzadeh H, Noraei NB. 2009. Anxiolytic and hypnotic effect of Crocus sativus aqueous extract and its constituents, crocin and safranal, in mice. Phytother Res, 23: 768-774.

Huang, F, Xiong Y, Xu L, Ma S, Dou C. 2007. Sedative and hypnotic activities of the ethanol fraction from Fructus schisandrae in mice and rats. J Ethnopharmacol, 110 : 471-475.

Johnston GAR. 2005. GABAA receptor channel pharmacology, Curr Pharm Des, 11: 1867–1885.

Kumar P, Gupta S, Murugan P, Singh SB. 2009. Ethnobotanical studies of Nubra Valley - A cold arid zone of Himalaya. Ethnobot Leaflets, 13: 752 – 765.

 Lee C.M., Wong H.N.C.,  Chui K.Y, Choang T.F., Hon P.M, Chang H.M. 1991. Miltirone,  a central benzodiazepine receptor partial agonist from a Chinese medicinal herb Salvia Miltiorrhiza. Neurosci Lett, 127: 237-241.

Mahboubi M, Kazempour N. 2009. The antimicrobial activity of essential oil from Perovskia abrotanoides karel and its main components. Indian J  Pharm Sci, 71: 343 – 347.

Mazandarani M, Ghaemi E. 2010. Ethnopharmacological investigation of different parts of Perovskia abrotanoides Karel. Planta Medica, 76: 123 –125.

Meletis C. D., Zabriskie N. 2008. Natural approaches for optimal sleep. J Altern Complement Med, 14: 181–188.

Moallem SA, Niapour M. 2008. Study of embryotoxicity of Perovskia abrotanoides, an adulterant in folk medicine, during organogenesis in mice. J Ethnopharmacol, 117: 108 – 114.

Morteza-Semnani  K. 2004.  The  essential oil  composition  of  Perovskia  abroatanoides from Iran. Pharm Biol, 42: 214-216.

Mozaffarian, V.A. 1998. Dictionary of Iranian Plant Names. Farhang Moaser, Tehran, 400-401.

Nassiri AM, Parvardeh S, Niapour M, Hosseinzadeh H. 2000. Antinociceptive and anti-inflammatory effects of Perovskia abrotanoides aerial part extracts in mice and rats.  J Med Plants, 1:  25 – 33.

Oliaee D, Boroushaki MT, Oliaee N, Ghorbani A. 2014. Evaluation of cytotoxicity and antifertility effects of Artemisia Kopetdaghensis. Adv Pharmacol Sci, 745-760.

Orzel-Gryglewska J. 2010. Consequences of sleep deprivation. Int J Occup Med. Environ Health, 23: 95-114.

Pritchett K, Mulder GB. 2003. The rotarod. Contemp Top Lab Anim Sci, 42: 49.

Rakhshandah H, Hosseini M. 2006. Potentiation of pentobarbital hypnosis by Rosa damascena in mice. J Exp Biol, 44: 910-912.

Rakhshandeh H, Sadeghnia H.R, Ghorbani  A. 2011. Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice. Nat Prod Res, 26: 2095-2098.

Rustaiyan AH, Masoudi S, Ameri N, Samiee K, Monfared A. 2006. Volatile constituents of Ballota aucheri Boiss, stachys benthamiana Boiss and Perovskia abrotanoides Karel, Growing wild in Iran. J Essent Oil Res, 18: 218-221.

 Sairafianpour M, Christensen J, Staerk D, Budnik BA, Kharazmi A, Bagherzadeh K, Jaroszewski JW. 2001. Leishmanicidal, antiplasmodial, and cytotoxic activity of novel diterpenoid 1,2-quinones from Perovskia abrotanoides: new source of tanshinones. J Nat Products, 64: 1398 – 1403.

 Sajjadi  SE.,  Mehregan  I.,  Khatamsaz M. , Asghari  G.H.  2005. Chemical composition  of  the  essential  oil  of  Perovskia abroatanoides Karel growing wild in Iran. Flavour Frag J, 20: 445-446.

Shah AJ, Rasheed M, Jabeen Q, Ahmed A, Tareen RB, Gilani AH, Nadir M, Ahmad VU. 2013. Chemical analysis and calcium channel blocking activity of the essential oil of Perovskia abrotanoides. Nat Prod Commun, 8: 1633-1636.

Tareen RB, Bib T, Khan MA, Ahmad M, Zafar M. 2010. Indigenous knowledge of folk medicine by the women of Kalat and Khuzdar Regions of Balochistan, Pakistan. Pakistan J Bot, 42: 1465 – 1485.

Vallières A, Ivers H, Bastien CH, Beaulieu-Bonneau S, Morin CM. 2005. Variability and predictability in sleep patterns of chronic insomniacs. J Sleep Res, 14: 447–453.

Zaharna M, Guilleminault C. 2010. Sleep, noise and health: review. Noise Health, 12: 64-69.

Vafaee F, Hosseini M, Sadeghinia HR, Hadjzadeh MA, Soukhtanloo M, Rahimi M. 2014. The effects of soy extract on spatial learning and memory damage induced by global ischemia in ovariectomised rats. Malays J Med Sci, 21: 19-30.

Zhao X, Cui XY, Chu QP, Chen BQ, Wang XM, Lin ZB. 2006. Potentiating effects of L-type Ca(2+)  channel  blockers  on  pentobarbital-induced hypnosis  are  influenced  by  serotonergic  system. J Neural Transm,  113: 1395-1402.