Anti-inflammatory effect of Fumaria parviflora leaves based on TNF-α, IL-1, IL-6 and antioxidant potential

Document Type: Original Research Article

Authors

1 Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh 202002, India

2 Department of Pharmacology, Raichur Institute of Medical Sciences, Raichur, 584101, India

3 Chemical Research Unit, Department of research in Unani Medicine, Aligarh Muslim University, Aligarh 202002, India

4 Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh 202002, India

5 Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh 202002, India

Abstract


Objective: In this study, we evaluated anti-inflammatory activity of leaves of Fumaria parviflora (F. parviflora) and underlying mechanisms by using in vivo models of inflammation.

Material and Methods: Albino Wistar rats of either sex weighing 150 - 200 g were used. Soxhlet ethanol and aqueous extracts of leaves of F. parviflora (EFP and AFP) were prepared. The anti-inflammatory activity was studied using carrageenan-induced paw edema method and cotton pellet granuloma method. Levels of cytokines such as TNF-α, IL-6 and IL-1 and activity of antioxidant enzymes including catalase (CAT) and glutathione peroxidase (GPx) were estimated.

Results: Leaves of F. parviflora demonstrated significant (p<0.001) decrease in paw edema in carrageenan-induced paw edema method. It diminished the serum tumour necrosis factor-α (TNF-α), IL-6 and IL-1 levels and also significantly attenuated the malondialdehyde (MDA) levels. The activity of CAT and GPx was increased in paw tissue. It also demonstrated significant decrease in granuloma formation in cotton pellet-induced granuloma method.

Conclusion: Leaves of F. parviflora possess anti-inflammatory activity as they inhibit various cytokines and have antioxidant effects and free radical scavenging activity.

Keywords

Main Subjects


Amin GH. 1991. Popular Medical Plants of Iran: Farhang Publication, Tehran, pp. 51-2.

Armstrong D, Browne R.1994. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds related to oxidative stress as applied to the clinical chemistry laboratory. Adv Exp Med Biol, 366: 43-58.

Bignotto L, Rocha J, Sepodes B, et al. 2009. Anti-inflammatory effect of lycopene on carrageenan-induced paw oedema and hepatic ischaemia-reperfusion in the rat. Br J Nutr, 102: 126-33.

Chang CT, Huang SS, Lin  SS. 2011. Anti-inflammatory activities of tormentic acid from suspension cells of Eriobotrya japonica ex vivo and in vivo. Food Chem, 127: 1131–7.

Chaturvedi P. 2008. Inhibitory response of Raphanus sativus on lipid peroxidation in albino rats. Evid Based Complement Alternat Med, 5: 55-9.

Dawson J, Sedgwick AD, Edwards JC, et al. 1991. A comparative study of the cellular, exudative and histological responses to carrageenan, dextran and zymosan in the mouse. Int J Tissue React,13:171-85.

Gilani AH, Janbas KH, Akhtar MS. 1996. Selective effect of an extract from Fumaria parviflora on paracetamol-induced hepatotoxicity. Gen Pharmacol, 27; 979-983.

Gohil AN,  Daniel M. 2014.  Identification of pharmacognostic and phytochemical biomarkers to distinguish between Fumariaparviflora Lam. and its adulterant. Rungiarepens (L.) Nees, 3: 58-64.

Hamedeyazdan S, Fathiazad F, Nazemiyeh H,  Faezi SA. 2012. Phytochemical analysis and antioxidant activity of Fumaria parviflora. Res Pharm Sci, 7: 762.

Heidari MR, Mandgary A, Enayati M. 2004. Antinociceptive effects and toxicity of Fumaria parviflora Lam. in mice and rats. Daru,12: 136-140.

Heydari Nasrabadi M, Aboutalebi H, Naseri M. 2012. Effect of Fumaria parviflora alcoholic extract on male rat reproductive system. J Med Plants Res, 10; 2004-2010.

Huang GJ, Pan CH, Wu CH. 2012. Sclareol exhibits anti-inflammatory activity in both lipopolysaccharide-stimulated macrophages and the λ-carrageenan-induced paw edema model. J Nat Prod, 75: 54-59.

Jamshidzadeh A, Niknahad HX. 2006. Hepatoprotective effects of Fumaria parvifloraon CCL4-induced hepatotoxicity. J Med Plants, 19: 34-39.

Jowkar F, Jamshidzadeh A, MirzadehYazdi A et al. 2011. The effects of Fumaria parviflora extract on chronic hand eczema: A randomized double-blind placebo controlled clinical trial. Iran Red Crescent Med J, 11: 824-828.

Lee YM, Seon MR, Cho HJ, et al. 2009. Benzyl isothiocyanate exhibits anti-inflammatory effects in murine macrophages and in mouse skin. J Mol Med, 87:1251-1261.

Liao CH, Guo SJ, Lin JY. 2011. Characterisation of the chemical composition and in vitro anti-inflammation assessment of a novel lotus (Nelumbo nucifera Gaertn) plumulepolysaccharide. Food Chem, 125: 930-935.

Lowry OH, Rosebrough NJ, Farr AL, et al. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem, 193: 265–267.

Naz I, Palomares-Rius JE, Blok SV, et al. 2013. In vitro and in plantanematicidal activity of Fumaria parviflora (Fumariaceae) against the southern root-knot nematode Meloidogyne incognita. Plant Pathol, 62: 943-952.

Ohishi N, Ohkawa H, Miike A, et al. 1985.  A new assay method for lipid peroxides using a methylene blue derivative. BiochemInt, 10:205-11.

Orhan I, Sener B, Musharraf SG. 2010. Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol, 64: 205–209.

Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70:158-169.

Raj Narayana K, Sripal Reddy K, Chaluvadi MR, et al. 2001. Bioflavonoids Classification, Pharmacological, Biochemical Effects and Therapeutic Potential. India J Pharmacol, 33: 2-1.

Rao CV, Verma AR, Gupta PK, et al. 2007. Anti-inflammatory and anti-nociceptive activities of Fumaria indica whole plant extract in experimental animals. Acta Pharm, 57: 491-498.

Sousek J, Vavreckova C, Psotova J, et al. 1999. Antioxidant and antilipoperoxidant activities of alkaloid and phenolic extracts of eight Fumaria species.Acta Hort, 501: 239-44.

Swingle KF, Shideman FE. 1972. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Therap, 13: 226–34.

Vinegar R, Schreiber W, Hugo R. 1969. Biphasic development of carrageenin edema in rats. J Pharmacol Exp, 166: 96–103.

Winter CA, Porter CC. 1957. Effect of alterations in side chain upon anti-inflammatory and liver glycogen activities of hydrocortisone esters. J Am Pharm Assoc Am Pharm Assoc (Baltim), 46: 515-519.

Winter CA, Risley EA, Nuss GW. 1962. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med, 111: 544-7

Zargari A. 1989. Medicinal Plants. Tehran University Publication, Tehran, pp.170-71.