Hepatoprotective activity of the extract of Homalium letestui stem against paracetamol-induced liver injury

Document Type: Original Research Article

Authors

Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria

Abstract

Homalium letestui Pellegr (Flacourtiaceae) is used traditionally by the Ibibios of Southern Nigeria to treat stomach ulcer, malaria and other inflammatory diseases and Yorubas of western Nigeria as an antidote. The hepatoprotective effect of the stem extract (200-600 mg/kg) was evaluated by the assay of liver function parameters, namely total and direct bilirubin, serum protein and albumin, total cholesterol, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), and alkaline phosphatase activities (ALP), antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and histopathological study of the liver. GCMS analysis of n-butanol fraction was carried out. Administration of the stem extract caused a significant (p

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Adams RP. 2001. Identification of Essential oils by Gas Chromatography Quadrupole Mass Spectrometry. Allured Publishing Corporation, Carol Stream, USA

Adeneye AA, Olagunju JA, Benebo AS, Elias SO, Adisa AO, Idowu BO, Oyedeji MO, Isioye EO, Braimoh OB, Oladejo OO, Alana EO. 2008. Nephroprotective effects of the aqueous root extract of Harungana madagascariensis [L.] in acute and repeated dose acetaminophen renal injured rats. Int J Appl Res Nat Prod,1: 6–14.

Aldridge WN. 1981. Mechanism of toxicity: New concepts are required in toxicology. Trends in Pharmacol Sci 2: 228–231.

Amazu LU, Okokon JE, Nwidu LL. 2015. Antiulcer activity of stem extract and fractions of Homalium letestui. Int J Pharmacog 2: 242-247.

Antia BS, Okokon JE, Nwidu LL. 2015. Antidiarrhoeal activity of stem extract of Homalium letestui. Int J Phytother 5: 86-89.

Cooper RA, Durocher JR, Leslie MH. 1977. Decreased fluidity of red cell membrane lipids in a beta lipoproteinemia. J Clin Investig 60: 115-121.

Dahlin D, Miwa G, Lee A. 1984. N-acetyl-pbenzoquinonamine: A cytochrome P450 dependent oxidation product of acetaminophen. Proc Natl Acad Sci. 81: 327 - 331.

Ellman GL. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys. 82, 70-77.

Gilani AH, Jabeen Q, Ghayur MN, Janbaz KH, Akhtar MS. 2005. Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the Carum copticum seed extract. J Ethnopharmacol 98: 127–135.

Halliwell B, Gutteridge JM.1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J.  219: 1–14.

Hutchinson T, Daziel JM. 1963. Flora of West Tropical Africa, Vol 2. Crown Agents for overseas government. London.

Ita B, Ngochindo R. 2014. Fatty Acid Composition, antioxidant and antimicrobial activity of Homalium letestui Stem. Austral J Basic Appl Sci.  8: 416-422.

Kamat, JP, Ghosh A, Devasagayam TP. 2000. Vanillin as an antioxidant in rat liver mitochondria: inhibition of protein oxidation and lipid peroxidation induced by photosensitization. Mol Cell Biochem. 209: 47 - 53.

Keay RWJ. 1989. Trees of Nigeria. A revised version of Nigerian trees (Vol 1 and 2), Keay RWJ, Onoche CFA, Stanfield DP (eds). Clarendon Press: Oxford.

Kumar S, Priyadarsini K, Sainis K. 2002. Free radical scavenging activity of vanillin and o-vanillin using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Redox Rep. 7:35 - 40.

Lawrence RA, Burk RF 1976.Glutathione peroxidase activity in selenium- deficient rat liver. BiochemBiophys Res Commun. 71: 952-958.

Lin CC, Shieh DE, Yen MH. 1997. Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J Ethnopharmacol 56: 193–200.

Lirdprapamongkol K, Kramb JP, Suthiphongchai T, Surarit R, Srisomsap C, Dannhardt G, Svasti J. 2009. Vanillin suppresses metastatic potential of human cancer cells through PI3K inhibition and decreases angiogenesis in vivo. J Agr Food Chem. 57: 3055 - 3063.

Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R, Karthikeyan D, Mallegaswari R. 2008. Hepatoprotective activity of Aerva lanata Linn.against paracetamol induced hepatotoxicity in rats. Res J Pharm Technol 1: 398 – 400.

Marklund, S., Marklund, G. 1974.Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase.Eur J Biochem. 47:469-474.

Martin P, Friedman LS. 1992. Assessment of liver function and diagnostic studies. In: Friedman LS, Keeffe EB. (Eds.), Hand Book of Liver Disease. Churchill Livingstone, Philadelphia, pp. 1–14.

Muriel, P., Garcipiana, T. 1992. Silymarin protects against paracetamol-induced lipid peroxidation and liver damage. J ApplToxicol. 12: 439-442.

Nkosi CZ, Opoku AR, Terblanche SE. 2005. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low protein fed rats. Phytother Res 19: 341–345.

Okokon JE, Davies K. 2014. Psychopharmacological studies of Homalium letestui stem  extract. J Pharmaceut Biol. 4: 158 – 164.

Okokon JE, Antia BS, Ita BN. 2007. Antidiabetic effects of Homalium letestui (Flacourtiaceae) in streptozotocin induced diabetic rats. Res J Med Plants. 1: 134 - 138.

Okokon JE, Dar A, Choudhary MI. 2013b. Cellular antioxidative, anticancer and antileishmanial activities of Homalium letestui.AviccenaJ  Phytomed. 3: 35 - 44.

Okokon JE, Ita BN, Udokpoh AE. 2006. Antimalarial activity of Homalium letestui. Phytother Res. 20: 949 - 951.

Okokon JE, Okokon PJ, Dar A., Choudhary MI. 2013a.  Antiinflammatory  and analgesic activities of Homalium letestui. Pharm Biol 51: 1459 – 1466.

Plaa GL, Hewitt WR. 1989. Detection and evaluation of chemically induced liver injury. In: Wallace Hayes, A. (Ed.), Principles and Methods of Toxicology. Raven Press, New York, pp. 399–628.

Prakash J, Gupta SK, Kochupillai V, Singh N, Gupta YK, Joshi S. 2001. Chemopreventive activity of Withania somnifera in experimentally induced fibrosarcoma tumours in Swiss albino mice. Phytother Res. 15: 240 - 244.

Reitman S, Frankel S. 1957. Determination of Glutamate-Pyruvate transaminase (ALT) and Aspartate Aminotransfrase (AST).J ClinPathol.28: 56.

Setzer WN, Stokes SL, Penton AF, Takaku S, Haber WA, Hansell E,  Caffrey CR, McKerrow JH. (2007). Cruzain Inhibitory activity of leaf essential oils of neotropicallauraceae and essential oil components. Nat Prod Comm. 2: 1203–1210.

Shashank T, EllandalaR,Yasmeen N, Sujatha K, Vishal Reddy k. 2011. Evaluation of hepatoprotective activity of stem bark of Homalium zeylanicum in rats. Int J Pharm Tech Res. 3 : 1630-1634.

Sinha AK. 1972. Colorimetric assay of catalase. Analyt Biochem. 47: 389 - 94.

Streeter AJ, Dahlin DC, Nelson SD, Baillie TA. 1984. The covalent binding of acetaminophen to protein. Evidence for cysteine residues as major sites of arylation in vitro.Chem Biol Interact  48: 349-66.