Origanum vulgare leaf extract protects mice bone marrow cells against ionizing radiation

Document Type: Original Research Article


1 Department of Radiology, School of Allied, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Anatomy, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Objective: Ionizing radiation produces free radicals which induce DNA damage and cell death. Origanum vulgare leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers. In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irradiation has been investigated in mice bone marrow cells.

Materials and Methods: OVLE was injected intraperitoneally to the BALB/c mice 1hr prior to gamma irradiation (3Gy) at the doses of 100 and 200 mg/kg. Twenty four hours after irradiation or treatment, animals were killed and smears were prepared from the bone marrow cells. The slides were stained with May Grunwald–Giemsa method and analyzed microscopically. The frequency of micronucleated polychromatic erythrocytes (MnPCEs), micronucleated normochromatic erythrocyte (MnNCEs) and cell proliferation ratio PCE/PCE+NCE (polychromatic erythrocyte/polychromatic erythrocyte + normochromatic erythrocyte) were calculated.

Results: The results showed that gamma irradiation (3Gy) increased the frequency of MnPCEs, MnNCEs and  reduced the PCE/PCE+NCE ratio in mice bone marrow compared to the non-irradiated control group (p< 0.0001). Injection of OVLE significantly reduced the frequency of MnPCEs (p< 0.0001) and MnNCEs (p< 0.05) and increased the PCE/PCE+NCE ratio as compared to the irradiated control group (p< 0.05).

Conclusion: It seems that OVLE with its antioxidant properties and its capability of scavenging free radicals and reactive oxygen species can reduce the cytotoxic effects of gamma irradiation in mice bone marrow cells.


Main Subjects

Arami S, Ahmadi A, Haeri SA. 2013. The radioprotective effects of Origanum vulgare extract against genotoxicity induced by 131I in human blood lymphocyte. Cancer Biother. Radiopharm, 28: 201-206.

Archana P, Rao BN, Ballal M, Rao BS.  2009. Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity.  Mutat Res Genet Toxicol Environ Mutagen, 680: 70-77.

Brown DQ, Pittock JW, Rubinstein JS. 1982. Early results of the screening program for radioprotectors. Int J Radiat Oncol Biol Phys, 8: 565-570.

Burt SA, van der Zee R, Koets AP, de Graaff AM, van Knapen F, Gaastra W, et al. 2007. Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Escherichia coli O157: H7. J Appl Environ Microbiol,73: 4484-4490. 

Cassatt DR, Fazenbaker CA, Bachy CM, Hanson MS. 2002. Preclinical modeling of improved amifostine (Ethyol) use in radiation therapy. Semin Radiat Oncol,12 (1 Suppl 1):97-102.

Ceker S, Agar G, Nardemir G, Anar M, Kizil HE, Alpsoy L. 2012. Investigation of anti-oxidative and anti-genotoxic effects of Origanum vulgare L. essential oil on human lymphocytes in vitro. J Essent  Oil Bear  Pl,15: 997-1005.

Chen JH, Ho C-T. 1997. Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds.  J Agr Food Chem,45: 2374-2378.

De Martino L, De Feo V, Formisano C, Mignola E, Senatore F. 2009. Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart growing wild in Campania (Southern Italy).  Molecules,14: 2735-2746.

Faleiro L, Miguel G, Gomes S, Costa L, Venâncio F, Teixeira A. 2005. Antibacterial and antioxidant activities of essential oils isolated from Thymbra capitata L.(Cav.) and Origanum vulgare L. J Agr Food Chem,53: 8162-8168.

Held KD, Biaglow JE. 1994. Mechanisms for the oxygen radical-mediated toxicity of various thiol-containing compounds in cultured mammalian cells.  J Radiat Res,139: 15-23.

Hosseinimehr S, Nemati A. 2014. Radioprotective effects of hesperidin against gamma irradiation in mouse bone marrow cells. Brit J Radiol, 79: 415–418.

Hosseinimehr S. J. 2007. Trends in the development of radioprotective agents. Drug Discov Today,12: 794-805.

Hosseinimehr SJ, Azadbakht M, Mousavi SM, Mahmoudzadeh A, Akhlaghpoor S. 2007. Radioprotective effects of hawthorn fruit extract against gamma irradiation in mouse bone marrow cells. J Radiat Res,48: 63-68.

Hosseinimehr SJ, Tavakoli H, Pourheidari G, Sobhani A, Shafiee A. 2003. Radioprotective Effects of Citrus Extract Against γ;-Irradiation in Mouse Bone Marrow Cells.  J Radiat Res,44: 237-241.

Ipek E, Zeytinoglu H, Okay S, Tuylu BA, Kurkcuoglu M, Baser KHC. 2005. Genotoxicity and antigenotoxicity of Origanum oil and carvacrol evaluated by Ames Salmonella/microsomal test. Food Chem,93: 551-556.

Jagetia G, Baliga MS, Malagi K, Kamath MS. 2002. The evaluation of the radioprotective effect of Triphala (an ayurvedic rejuvenating drug) in the mice exposed to γ-radiation. Phytomedicine,9: 99-108.

Jagetia G, Venkatesh P, Baliga M. 2004. "Evaluation of the radioprotective effect of  bael leaf (Aegle marmelos) extract in mice. Int  J Radiat Biol  ,80: 281-290.

Jagetia GC, Venkatesha V, Reddy TK. Naringin. 2003. Naringin, a citrus flavonone, protects against radiation-induced chromosome damage in mouse bone marrow. Mutagenesis,18: 337-343.

Kapiszewska M, Soltys E, Visioli F, Cierniak A, Zajac G. 2005. The protective ability of the Mediterranean plant extracts against the oxidative DNA damage. The role of the radical oxygen species and the polyphenol content.  J Physio Pharmacol. Supplement,56: 183-197.

Karakaya S, El SN, Karagözlü N, Şahin S. 2011. Antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) by using different extraction methods.  J  Med Food,14: 645-652.

Karbownik M, Reiter RJ. 2000. Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. P Soc Exp Biol Med,225: 9-22.

Khan A, Manna K, Das DK, Sinha M, Kesh SB, Das U, et al. 2014. Seabuckthron (Hippophae rhamnoides L.) leaf extract ameliorates the gamma radiation mediated DNA damage and hepatic alterations. Indian J Exp Biol, 52: 952-964.

Kulisic T, Radonic A, Katalinic V, Milos M. 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem,85: 633-640.

Lagouri V, Boskou D. 1996. Nutrient antioxidants in oregano. Int J Food  Sci Nutr,47: 493-497.

Lambert R, Skandamis PN, Coote PJ, Nychas GJ. 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol.  J Appl Microbiol,91: 453-462.

Landauer MR, Srinivasan V, Seed TM. 2003. Genistein treatment protects mice from ionizing radiation injury. J  App Toxicol, 23: 379-385.

Larson RA. 1988. The antioxidants of higher plants.  Phytochemistry, 27: 969-978.

Lee S, Buber M, Yang Q, Cerne R, Cortes R, Sprous D, et al. 2008. Thymol and related alkyl phenols activate the hTRPA1 channel. Brit J Pharmacol,153: 1739-1749.

Lee T-K, Johnke RM, Allison RR, O'Brien KF, Dobbs LJ. 2005. Radioprotective potential of ginseng. Mutagenesis,20: 237-243.

Mezzoug N, Elhadri A, Dallouh A, Amkiss S, Skali N, Abrini J, et al. 2007. Investigation of the mutagenic and antimutagenic effects of Origanum compactum  essential oil and some of its constituents. Mutat Res-Gen Toxen,629: 100-110.

Nakatani N. 1992. Natural antioxidants from spices. ACS symposium series (USA).

Niture SK, Rao US, Srivenugopal KS. 2006. Chemopreventative strategies targeting the MGMT repair protein: augmented expression in human lymphocytes and tumor cells by ethanolic and aqueous extracts of several Indian medicinal plants. Int  J Oncol,29: 1269-1278.

Özbek T, Guelluece M, Şahin F, Oezkan H, Sevsay S, Bariş Ö. 2008. Investigation of the antimutagenic potentials of the methanol extract of Origanum vulgare L. subsp. vulgare in the Eastern Anatolia Region of Turkey. Turk J  Biol,32: 271-276.

Roofchaee A, Irani M, Ebrahimzadeh MA, Akbari MR. 2013. Effect of dietary oregano (Origanum vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens. Afr  J Biotechnol,10: 6177-6183.

Padulosi S. 1997.  Oregano, Proceeding of the IPGRI International Workshop on Oregano. Rome, Italy: pp: 84 - 86.

Samarth R, Goyal P, Kumar A. 2004.  Protection of swiss albino mice against whole‐body gamma irradiation by Mentha piperita (Linn.). Phytother Res,18: 546-550.

Schmid W. 1975. The micronucleus test. Mutat Res-Envir  Muta, 31: 9-15.

Sharma P, Parmar J, Sharma P, Verma P, Goyal P. 2011. Radiation-induced testicular injury and its amelioration by Tinospora cordifolia (An Indian medicinal plant) extract. Evid-Based Complement Alternat Med. 2011:643847. doi: 10.1155/2011/643847.

Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S, Lanaras T, Arsenakis M. 1996. Antimicrobial and cytotoxic activities of Origanum essential oils. J Agr Food Chem,44: 1202-1205.

Teissedre P, Waterhouse A. 2000. Inhibition of oxidation of human low-density lipoproteins by phenolic substances in different essential oils varieties. J Agr Food Chem,48: 3801-3805.

Vicuña GC, Stashenko EE, Fuentes JL. 2010. Chemical composition of the Lippia origanoides essential oils and their antigenotoxicity against bleomycin-induced DNA damage. Fitoterapia, 81: 343-349.

Whitnall MH, Elliott TB, Harding RA, Inal CE, Landauer MR, Wilhelmsen CL, et al. 2000. Androstenediol stimulates myelopoiesis and enhances resistance to infection in gamma-irradiated mice. Int J Immunopharmaco,22: 1-14.