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Avicenna Journal of Phytomedicine
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Absalan, A., Mesbah-Namin, S., Tiraihi, T., Taheri, T. (2016). The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study. Avicenna Journal of Phytomedicine, 6(6), 643-657. doi: 10.22038/ajp.2016.6785
Abdorrahim Absalan; Seyed Alireza Mesbah-Namin; Taki Tiraihi; Taher Taheri. "The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study". Avicenna Journal of Phytomedicine, 6, 6, 2016, 643-657. doi: 10.22038/ajp.2016.6785
Absalan, A., Mesbah-Namin, S., Tiraihi, T., Taheri, T. (2016). 'The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study', Avicenna Journal of Phytomedicine, 6(6), pp. 643-657. doi: 10.22038/ajp.2016.6785
Absalan, A., Mesbah-Namin, S., Tiraihi, T., Taheri, T. The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study. Avicenna Journal of Phytomedicine, 2016; 6(6): 643-657. doi: 10.22038/ajp.2016.6785

The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study

Article 6, Volume 6, Issue 6, November 2016, Page 643-657  XML PDF (1453 K)
Document Type: Original Research Article
DOI: 10.22038/ajp.2016.6785
Authors
Abdorrahim Absalan1; Seyed Alireza Mesbah-Namin 1; Taki Tiraihi2; Taher Taheri3
1Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Abstract

Objective: The aim of this study was to estimate the cheminformatics and qualitative structure-activity relationship (QSAR) of cinnamaldehyde and eugenol. The effects of cinnamaldehyde and eugenol on the viability, doubling time and adipogenic or osteogenic differentiations of human adipose-derived mesenchymal stem cells (hASCs) were also investigated. 

Materials and Methods: QSAR and toxicity indices of cinnamaldehyde and eugenol were evaluated using cheminformatics tools including Toxtree and Toxicity Estimation Software Tool (T.E.S.T) and molinspiration server. Besides, their effects on the hASCs viability, doubling time and differentiation to adipogenic or osteogenic lineages were evaluated.

Results: Cinnamaldehyde is predicted to be more lipophilic and less toxic than eugenol. Both phytochemicals may be developmental toxicants. They probably undergo hydroxylation and epoxidation reactions by cytochrome-P450. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol did not influence hASCs viability following 72 hr of treatment. But higher concentrations of these phytochemicals insignificantly increased hASCs doubling time till 96 hr, except 1 µg/ml eugenol for which the increase was significant. Only low concentrations of both phytochemicals were tested for their effects on the hASCs differentiation. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol enhanced the osteogenesis and decreased the adipogenesis of hASCs meaningfully.

Conclusion: According to the cheminformatics analysis and in vitro study, cinnamaldehyde and eugenol are biocompatible and low toxic for hASCs. Both phytochemicals may be suitable for regenerative medicine and tissue engineering when used at low concentrations, but maybe useful for neoplastic growth inhibition when used at high concentrations.
Keywords
Stem cell; Cell viability; Quantitative Structure-Activity Relationship; Cinnamaldehyde; Eugenol
Main Subjects
Toxicology
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