The effects of crocin and safranal on the yawning induced by intracerebroventricular injection of histamine in rats

Document Type: Original Research Article

Authors

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran

Abstract


Objective: Crocin and safranal, as the major constituents of saffron, have many biological activities. This study investigated the effects of crocin and safranal on yawning response induced by intracerebroventricular (i.c.v.) injection of histamine in rats.
Materials and Methods: In ketamine/xylazine-anesthetized rats, a guide cannula was implanted in the right ventricle of the brain and yawning induced by i.c.v. injection of histamine. Crocin and safranal were intraperitoneally (i.p.) injected alone and before i.c.v. injection of histamine.
Results: Histamine at the doses of 10 and 20 µg/rat produced yawning. Mepyramine (a histamine H1 receptor antagonist) 40 µg/rat significantly (p<0.05) prevented histamine (20 µg/rat)-induced yawning.  Crocin (30 mg/kg) and safranal (1 mg/kg) significantly (p<0.05) increased histamine (10 µg/rat)-induced yawning. Crocin and safranal also induced yawning when injected before mepyramine plus histamine administration.   
Conclusion: The results of the present study showed a yawning-inducing effect for central histamine, which was inhibited by mepyramine. Crocin and safranal increased histamine-induced yawning, and also produced yawning when the histamine action is blocked.

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Amin B, Hosseinzadeh H. 2012. Evaluation of aqueous and ethanolic extracts of saffron, Crocus sativus L, and its constituents, safranal and crocin in allodynia and hyperalgesia induced by chronic constriction injury model of neuropathic pain in rats. Fitoterapia, 83: 888-895.

Assimopolou AN, Sinakos Z, Papageorgiou VP. 2005. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res, 19: 997-1000.

Boskabady MH, Tabatabaee A, Byrami G. 2012. The effect of the extract of Crocus sativus and its constituent safranal, on lung inflammation of ovalbumin sensitized guinea-pigs. Phytomedicine, 19: 904-911.

Boskabady M.H, Rahbardar M.G, Jafari Z. 2011. The effect of safranal on histamine H1 receptors of guinea pig tracheal chains. Fitoterapia, 82: 162–167.

Collins GT, Eguibar JR. 2010. Neuropharmacology of yawning. Front Neurol Neurosci, 28: 90-106.

Collins GT, Witkin GM, Newman AH, Svensson KA, Grundt P, Cao J, Woods JH. 2005.  Dopamine-agonist induced yawning in rats: a dopamine D3 receptor-mediated behavior. J Pharmacol Exp Ther, 314: 310–319.

Ferrari F, Baggio G. 1985. Influence of cimetidine, ranitidine and imidazol on the behavioral effects of (+/-) N-n-propylnorapomorphine in male rats. Psychopharmacology (Berlin), 85: 197-200.

Gadrdoost B, Vafaei AA, Rashidy-Pour A, Hajisoltani R, Bandegi AR, Motamedi F, Haghighi S, Sameni HR, Pahlavan S. 2011. Protective effects of saffron extract and its active constituent crocin against oxidative stress and spatial learning and memory deficts induced by chronic stress in rats. Eur J Pharmacol, 667: 222-229.

Haas HL, Sergeeva OA, Selbach O. 2008. Histamine in the nervous system. Physiol Rev, 88: 1183-1241.

Li SM, Collins GT, Paul NM, Grundt P, Newman AH, Xu M, Grandy DK, Woods JH, Katz JL. 2010. Yawning and locomotor behavior induced by dopamine receptor agonists in mice and rats. Behav Pharmacol, 21: 171-181.

Nam KN, Park YM, Jung HJ, Lee JY, Min BD, Park SU, Jung WS, Cho KH, Kang I, Hong JW, Lee EH. 2010. Anti-inflammatory effects of crocin and crocetin in rats brain Epub ahead of print 11  microglial cells. Eur J Pharmacol, 648: 110-116.

Paxinos G, Watson C. 1997. The rat brain in stereotaxic coordinates. Compact Third Edition, Academic Press, San Diego.

Rios JL, Recio MC, Ginger RM, Manz S. 1996. An update review of saffron and its active constituents. Phytother Res, 10: 189-193.

Seki Y, Nakatani Y, Kita I, Sato-Suzuki I, Oguri M, Arita H. 2003. Light induces cortical activation and yawning in rats. Behav Brain Res, 140: 65–73.

Seki Y, Sato-Suzuki I, Kita I, Oguri M, Arita H. 2002. Yawning/ cortical activation induced by microinjection of histamine into the paraventricular nucleus of the rat. Behav Brain Res, 134: 75–82.

Schmidt M, Betti G, Hensel A. 2007. Saffron in phytotherapy: pharmacology and clinical uses. Wien Med Wochenschr, 157: 315-319.

Tamaddonfard E, Farshid AA, Ahmadian E, Hamidhoseyni A. 2013a. Crocin enhanced functional recovery after sciatic nerve crush injury in rats. Iran J Basic Med Sci, 16: 83-90.

Tamaddonfard E, Farshid AA, Asri-Rezaee S, Javadi S, Khosravi V, Rahman B, Mirfakhraee Z. 2013b. Crocin improved learning and memory impairments in streptozotocin-induced diabetic rats. Iran J Basic Med Sci, 16: 91-100.

Tamaddonfard E, Farshid AA, Eghdami K, Samadi F, Erfanparast A. 2013c. Comparison of the effects of crocin, safranal and diclofenac on local inflammation and inflammatory pain responses induced by carrageenan in rats. Pharmacol Rep, 65: 1272-1280

Tamaddonfard E. 2013d. Turmeric active substance, curcumin, enhanced apomorphine-induced yawning in rats. Avicenna J Phytomed, 3: 231-237

Tamaddonfard E, HamzehGooshchi N, Seiednejhad-Yamchi S. 2012a.Central effect of crocin on penicillin-induced epileptiform activity in rats. Pharmacol Rep, 64: 94-101.

Tamaddonfard E, Farshid AA, Hosseini L. 2012b. Crocin alleviates the local paw edema induced by histamine in rats. Avicenna J Phytomed, 2: 97-104.

Tamaddonfard E, Soraya H, Hamzeh-Gooshchi N. 2008a. Central interaction between physostigmine and histamine during yawning in rats. Pharmacol Rep, 60: 896–903

Tamaddonfard E, Khalilzadeh E, Hamzeh-Gooshchi N, Seiednejhad-Yamchi S. 2008b.Central effect of histamine in a rat model of acute trigeminal pain. Pharmacol Rep, 60: 219–224.

Walusinski O. 2009. Yawning in diseases. Eur Neurol, 62: 180-187.