1Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Physiology, School of Medicine, Jiroft University of medical Sciences, Jiroft, Iran
4Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5Mashhad Technical Faculty, Technical and Vocational University, Mashhad, Iran
6Department of Physiology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Objective: Neuroimmune factors contribute on the pathogenesis of sickness behaviors. Nigella sativa (NS) has anti-inflammatory, anti-anxiety and anti-depressive effects. In the present study, the effect of NS hydro-alcoholic extract on sickness behavior induced by lipopolysaccharide (LPS) was investigated. Materials and Methods: The rats were divided into five groups (n=10 in each): (1) control (saline), (2) LPS (1 mg/kg, administered two hours before behavioral tests), (3-5) LPS-Nigellasativa 100 , 200 and 400 mg/kg (LPS-NS 100, LPS-NS 200 and LPS-NS 400, respectively). Open- field (OF), elevated plus maze (EPM) and forced swimming test (FST) were performed. Results: In OF, LPS reduced the peripheral crossing, peripheral distance, total crossing and total distance compared to control (pConclusion: The results of the present study showed that the hydro-alcoholic extract of NS reduced the LPS-induced sickness behaviors in rats. Further investigations are required for better understanding the responsible compound (s) and the underlying mechanism(s).
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