Teratogenic effect of Lippia citriodora leaves aqueous extract in mice

Document Type: Original Research Article

Authors

1 Departments of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

2 Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 Medical Toxicology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

4 Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective: Safety of Lippia citriodora, as a herbal remedy, in pregnancy has not yet been evaluated. This study aimed to identify the effect of L. citriodora aqueous extract on pregnancy outcome in mice.
Materials and Methods: Timed-pregnant mice received doses of 0.5 g/kg/day L. citriodora aqueous extract or the vehicle control during organogenesis, intraperitoneally. Maternal body weights were measured throughout the pregnancy. The litters were examined for external malformations and skeletal abnormalities. Fetuses were stained with Alizarin red S and Alcian blue.
Results: There were no significant differences in mean maternal weight gain during pregnancy between groups. Also, no significant differences were observed in mean number of implantation, live and resorbed fetuses between control and treated groups. The prevalence of all types of deformity was low and similar to control group (%1.11).
Conclusion: The results of this study show that moderate consumption of L. citriodora as an infusion or tea appears to be safe to be used during pregnancy and does not have toxic effects on development of mouse embryo.

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Ansari M, Larijani K, Tehrani M S. 2012. Antibacterial activity of Lippa citriodora herb essence against MRSA Staphylococcus aureaus. AJMR, 6: 16-19.

Cohen-Kerem R, Koren G. 2003. Antioxidants and fetal protection against ethanol teratogenicity. I. Review of the experimental data and implications to humans. Neurotoxicol Teratol, 25: 1-9.

Dweck A. 2009. Toxicology of Essential Oils Reviewed. Personal Care: 65-77.

Fischer-Rasmussen W, Kjaer S K, Dahl C, Asping U. 1991. Ginger treatment of hyperemesis gravidarum. Eur J Obstet Gynecol Reprod Biol, 38: 19-24.

Gardner Z, McGuffin M. 2013.American herbal products association's botanical safety handbook, pp. 50-51, U.S, CRC press.

Hosseinzadeh H, Sadeghnia H R, Imenshahidi M, Fazly Bazzaz B S. 2009. Review of the pharmacological and toxicological tffects of Salvia leriifolia. Iran J Basic Med Sci, 12: 1-8.

Kimmel C A, Trammell C. 1981. A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. Stain Technol, 56: 271-3.

Masuda Y, Kikuzaki H, Hisamoto M, Nakatani N. 2004. Antioxidant properties of gingerol related compounds from ginger. Biofactors, 21: 293-6.

Mello F B, Jacobus D, Carvalho K, Mello J R. 2005. Effects of Lantana camara (Verbenaceae) on general reproductive performance and teratology in rats. Toxicon, 45: 459-66.

Moallem S A, Afshar M, Etemad L, Razavi B M, Hosseinzadeh H. 2013. Evaluation of teratogenic effects of crocin and safranal, active ingredients of saffron, in mice.Toxicol Ind Health, [Epub ahead of print].

Moradi M, Mehrafarin A, NaghdiBadi H. 2014. Phytochemical variations in lemon verbena (Lippia citriodora H.B.K.) plantlets affected by propagation methods and soil type. J Medicinal Plants, 13: 54-68.

Pascual M E, Slowing K, Carretero E, Sánchez Mata D, Villar A. 2001. Lippia: traditional uses, chemistry and pharmacology: a review. J Ethnopharmacol, 76: 201-214.

Pastorelli G, Rossi R, Corino C. 2012. Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets. Czech J Anim SciI, 57: 312-322.

Ponce-Monter H, Fernández-Martínez E, Ortiz M, Ramírez-Montiel M L, Cruz-Elizalde D, et al. 2010. Spasmolytic and anti-inflammatory effects of Aloysia triphylla and citral, in vitro and in vivo studies. J Smooth Muscle Res., 46: 309-19.

Quirantes-Pine R, Herranz-Lopez M, Funes L, Borras-Linares I, Micol V, et al. 2013. phenylpropanoids and their metabolites are the major compounds responsible for blood-cell protection against oxidative stress after administration of Lippia citriodora in Rats. Phytomedicine, 20: 1112-8.

Valentao P, Fernandes E, Carvalho F, Andrade P B, Seabra R M, et al. 2002. Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol Pharm Bull, 25: 1324-7.

Wells P G, Bhuller Y, Chen C S, Jeng W, Kasapinovic S, et al. 2005. Molecular and biochemical mechanisms in teratogenesis involving reactive oxygen species. Toxicol Appl Pharmacol, 207: 354-66.