Saffron supplements modulate serum pro-oxidant-antioxidant balance in patients with metabolic syndrome: A randomized, placebo-controlled clinical trial

Document Type: Original Research Article

Authors

1 Department of Anatomy and Cell biology, Birjand University of Medical Sciences, Birjand, Iran.

2 Pharmacological Research Center of Medicinal Plant, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.

3 Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.

4 Institute of Genetics and Developmental Biology, University of Chinese Academy of Sciences, Beijing, China.

5 Cardiovascular Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.

6 Division of Medical Education, Brighton & Sussex Medical School, Rm 342, Mayfield House, University of Brighton, BN1 9PH, United Kingdom

Abstract

Objectives: We have investigated the effect of a saffron supplement, given at a dose of 100 mg/kg, on prooxidant-antioxidant balance (PAB) in individuals with metabolic syndrome.
Materials and Methods: A randomized, placebo-controlled trial design was used in 75 subjects with metabolic syndrome who were randomly allocated to one of two study groups: (1) the case group received 100mg/kg saffron and (2) the placebo control group received placebo for 12 weeks. The serum PAB assay was applied to all subjects before (week 0) and after (weeks 6 and 12) the intervention.
Results: There was a significant (p=0.035) reduction in serum PAB between week 0 to week 6 and also from week 0 to week 12. 
Conclusion: Saffron supplements can modulate serum PAB in subjects with metabolic syndrome, implying an improvement in some aspects of oxidative stress or antioxidant protection.

Keywords

Main Subjects


Alamdari DH, Ghayour-Mobarhan M, Tavallaie S, Parizadeh MR, Moohebati M, Ghafoori F. 2008. Prooxidant-antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clin Biochem ,41:375-380.

Abe K, Sugiura M, Yamaguchi S, Shoyama Y, Saito H. 1999. Saffron extract prevents acetaldehyde-induced inhibition of long-term potentiation in the rat dentate gyrus in vivo. Brain Res, 851:287-289.

Ahmad AS, Ansari MA, Ahmad M, Saleem S, Yousuf S, Hoda MN.2005. Neuroprotection by crocetin in a hemi-parkinsonian rat model. Pharmacol Biochem Behav, 81:805-813.

Bors W, Saran M, Michel C. 1982. Radical intermediates involved in the bleaching of the carotenoid crocin, Hydroxyl radicals, superoxide anions and hydrated electrons. Int J Radiat Biol Relat Stud Phys Chem Med, 41: 493- 501.

Eckel RH, Grundy SM, Zimmet PZ. 2005.The metabolic syndrome. Lancet, 365:1415-28.

Alberti KG, Zimmet P, Shaw J. 2006. Metabolic syndrome--a new world-wide definition. Diabet Med, 23: 469-480.

Edge R, McGarvey DJ, Truscott TG. 1997. The carotenoids as anti-oxidants-a review. J Photochem Photobiol B, 4: 189-200.

Falsoleiman H, Dehghani M, Moohebati M.. 2011. Changes in prooxidant- antioxidant balance after bare metal and drug eluting stent implantation in patients with stable coronary disease. Clin Biochem, 44: 160-164.

Gong W, Gottlieb S, Collins J, Blescia A, Dietz H, Goldmuntz E, McDonald-McGinn DM, Zackai EH, Emanuel BS, Driscoll DA, Budarf ML. 2001. Mutation analysis of TBX1 in non-deleted patients with features of DGS/VCFS or isolated cardiovascular defects. J Med Genet. , 38: E45.

Grundy SM. 2008. Metabolic syndrome pandemic. Arterioscler Thromb Vasc Biol, 28: 629-636.

Hosseinzadeh H, Modaghegh MH, Saffari Z. 2009. Crocus sativus L. (Saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle. Evid Based Complement Alternat Med, 6: 343-350.

He S-Y, Qian Z-Y, Wen N, Tang F-T, Xu G-L, Zhou C-H. 2007. Influence of Crocetin on experimental atherosclerosis in hyperlipidamic-diet quails. Eur J Pharmacol, 554: 191-195.

He S-Y, Qian Z-Y, Tang F-T, Wen N, Xu G-L, Sheng L. 2005. Effect of crocin on experimental atherosclerosis in quails and its mechanisms. Life Sci, 77: 907-921.

Kaminski KA, Bonda TA, Korecki J, Musial WJ. 2002. Oxidative stress and neutrophil activation--the two keystones of ischemia/reperfusion injury. Int J Cardiol, 86: 41-59.

Kotur-Stevuljevic J, Memon L, Stefanovic A, Spasic S, Spasojevic-Kalimanovska V, Bogavac-Stanojevic N. 2007. Correlation of oxidative stress parameters and inflammatory markers in coronary artery disease patients.Clin Biochem, 40: 181-187.

Lafont AM, Chai YC, Cornhill JF, Whitlow PL, Howe PH, Chisolm GM. 1995. Effect of alpha-tocopherol on restenosis after angioplasty in a model of experimental atherosclerosis.J Clin Invest, 95: 1018-1025.

Nair SC, Salomi MJ, Varghese CD, Panikkar B, Panikkar KR. 1992. Effect of saffron on thymocyte proliferation, intracellular glutathione levels and its antitumor activity. Biofactors, 4: 51-54.

Nair SC, Pannikar B, Panikkar KR. 1991.Antitumour activity of saffron (Crocus sativus). Cancer Lett, 57: 109-1014.

Nesto RW. 2003. The relation of insulin resistance syndromes to risk of cardiovascular disease. Rev Cardiovas Med, 4: S11.

Nunes GL, Sgoutas DS, Redden RA, Sigman SR, Gravanis MB, King SB, III. 1995. Combination of vitamins C and E alters the response to coronary balloon injury in the pig. Arterioscler Thromb Vasc Biol, 15: 156-65.

Papandreou MA, Tsachaki M, Efthimiopoulos S, Cordopatis P, Lamari FN, Margarity M. 2011 .Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behav Brain Res, 219 : 197-204.

Palozza P, Krinsky NI. 1992. Antioxidant effects of carotenoids in vivo and in vitro: an overview. Methods Enzymol, 213: 403-420.

Rahsepar AA, Mirzaee A, Moodi F, Moohebati M, Tavallaie S, Eshraghi A. 2012. Anti-Heat Shock Protein 27 Titers and Oxidative Stress Levels are Elevated in Patients WithValvular Heart Disease. Angiology, 63: 609-16.

Rı´os JL, RecioMC, Giner RM, Ma´nˇ ezS. 1996. An update review of saffron and its active constituents. Phtother Res, 10 : 189-193.

Sadeghnia HR, Kamkar M, Assadpour E, Boroushaki MT, Ghorbani A. 2013. Protective effect of safranal, a constituent of Crocus sativus, on quinolinic acid-induced oxidative damage in rat hippocampus. Iran J Basic Med Sci; 16: 73-82.

Tardif JC, Cote G, Lesperance J, Bourassa M, Lambert J, Doucet S. 1997. Probucol and multivitamins in the prevention of restenosis after coronary angioplasty. Multivitamins and Probucol Study Group. N Engl J Med, 337: 365-72.

Vassalle C, Petrozzi L, Botto N, Andreassi MG, Zucchelli GC. 2004. Oxidative stress and its association with coronary artery disease and different atherogenic risk factors. J Intern Med, 256: 308-15.

Walter MF, Jacob RF, Jeffers B, Ghadanfar MM, Preston GM, Buch J. 2004. Serum levels of thiobarbituric acid reactive substances predict cardiovascular events in patients with stable coronary artery disease: a longitudinal analysis of the PREVENT study. J Am Coll Cardiol, 44: 1996-2002.

Yoshino F, Yoshida A, Umigai N, Kubo K, Lee MC. 2011.Crocetin reduces the oxidative stress induced reactive oxygen species in the stroke-prone spontaneously hypertensive rats (SHRSPs) brain. J Clin Biochem Nutr, 49 : 182-7.

Zhang Y, Shoyama Y, Sugiura M, Saito H. Effects of Crocus sativus L. 1994.On the ethanol-induced impairment of passive avoidance performances in mice. Biol Pharm Bull, 17: 217-221.