Evaluation of hair growth promoting activity of Phyllanthus niruri

Document Type: Original Research Article

Authors

1 Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya Sagar- 470003 (M.P.),India

2 Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry, Charite Universitäts Medizin, Campus Virchow Klinikum, Augustenburgerplatz 1, Berlin, Germany

Abstract

Objectives: This study was designed to investigate the potential Phyllanthus niruri extracts in promotion of hair growth.
Materials and Methods: Here, we studied the hair growth promoting activity of petroleum ether extract of P. niruri following its topical administration. Alopecia was induced in albino rats by subcutaneous administration of testosterone for 21 days. Evaluation of hair loss inhibition was done by concurrent administration of extract and monitoring parameters like follicular density,  anagen/telogen (A/T) ratio and histological observation of animal skin sections. Finasteride solution was applied topically as standard. In vitro experiments were also performed to study the effect of extract on the activity of 5α-reductase enzyme
Results: Groups treated with petroleum ether extract of plant showed hair re-growth as reflected by follicular density, A/T ratio and skin sections. Histopathology and morphologic observations of hair re-growth at shaved sites showed active follicular proliferation. In vitro experiments results showed inhibitory activity of petroleum  ether extract on type-2 5α-reductase enzyme and an increase in the amount of testosterone with increasing concentrations. 
Conclusion: It could be concluded that petroleum ether extracts of P. niruri might be useful in the treatment of testosterone-induced alopecia in the experimental animal by inhibiting 5α-reductase enzyme.

Keywords

Main Subjects


Balawant S, William S, Goppinath K, Krishna B.1986. Isolation and Structure (X-Ray Analysis) of Ent-Norsecurinine, an Alkaloid from Phyllanthus niruri. J Nat Prod‏‏,49: 614-620

Bradford MM.1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem,72: 248-54.

Calixto B, Adair R, Valdir C, Rosendo AA.1998. A review of the plants of the genus Phyllanthus: Their chemistry, pharmacology, and therapeutic potential. Med Res Rev,18: 225-85.

Goodman LS and Gilman A.1996.The pharmacological basis of therapeutics. New York: McGraw Hills.

Headington JT, Novak E.1984. Clinical and histological studies of male pattern baldness treated with topical minoxidil. Curr Thera Res Clin Exp,36: 1098-1106.

Kakali D, Anu TS, Ashok M, Beena B, Ramesh B, Anand CB.2009. Eclipta alba extract with potential for hair growth promoting activity. J Ethnopharmacol, 124: 450-456.

Kaufman KD . 2002. Androgen and alopecia. Mol Cell Endocrinol 198: 89-95.

Matias JR, Malloy V, Orentreich N. 1989. Animal models of androgen-dependent disorders of the pilosebaceous apparatus. The androchronogenetic alopecia (AGA) mouse as a model for male-pattern baldness. Arch Dermatol Res, 281: 247-53.

Nutbrown M, Hull SP, Cunliffe WJ, Randal VA. 1995. Abnormalities in the ultrastructure of melanocytes and the outer root sheath of clinically normal hair follicles from alopecia areata scalps.  J Invest Dermatol, 104: 12S–13S.

Olsen EA. 1994. Androgenetic alopecia. In: Olsen EA, (Ed.) Disorders of hair growth: diagnosis and treatment. New York: McGraw-Hill.

Otberg N, Finner AM, Shapiro J. 2007. Androgenetic alopecia. Endocrinol Metab Clin North Am, 36: 379-398.

Pandit S, Chauhan NS, Dixit VK. 2008.  Effect of Cuscuta reflexa Roxb on androgen-induced alopecia. J Cosm Dermat, 7: 199-204.

Pérez-Ordeals V, Cabeza M, Bratoeff E et al .2005. New 5α-reductase inhibitors: in vitro and in vivo effects. Steroids 70: 217–24.

Purdon MP, Lehman-McKeeman LD. 1997. Improved high-performance liquid chromatographic procedure for the separation and quantification of hydroxytestosterone metabolites. J Pharmacol Toxicol Method, 37: 67-73.

Sperling LC . 2001. Hair and systemic disease. Dermatol Clin, 19 :711-726.

Steers WD .2001. 5α-Reductase activity in the prostate. Urology, 58: 17-24.

Unander D, Webster G, Blumberg B. 1991, Uses and bioassays in Phyllanthus (Euphorbiaceae): a compilation: II. The subgenus  Phyllanthus. J Ethnopharmacol, 34: 97-133.

Whiting DA, Waldstreicher J, Sanchez M, Kaufman KD. 1999. Measuring reversal of hair miniaturization in androgenetic alopecia by follicular counts in horizontal sections of serial scalp biopsies: results of finasteride 1 mg treatment of men and postmenopausal women. J. Invest. Dermatol. Symp. Proc., 4, 282-284.

Yoo HG, Kim JS, Lee SR, Pyo HK et al . 2006. Perifollicular fibrosis: Pathogenetic role in androgenetic alopecia. Biol Pharm Bull, 29: 1246-1250.