Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats

Document Type: Original Research Article


1 Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

2 Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

3 Department of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran

4 Department of basic sciences, College of veterinary medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran

5 Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

6 Tuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran


Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism.
Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed.
Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses.


Main Subjects

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