Growth inhibition and apoptosis induction of Scutellaria luteo-coerulea Bornm. & Sint. on leukemia cancer cell lines K562 and HL-60

Document Type: Original Research Article


1 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran.

2 Department of Pharmacognosy, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran


Objective: Scutellaria (Lamiaceae) has been implicated for medicinal purposes both in modern and traditional medicine. Some species of the genus Scutellaria has extensively been studied for anticancer activity. Scutellaria luteo-coerulea (S. luteo-coerulea) is one of the Iranian species of the genus Scutellaria.
Materials and Methods: In the present study, cytotoxic and apoptogenic properties of CH2Cl2, EtOAc, n-BuOH, and H2O fractions of S. luteo-coerulea were investigated on K562. Moreover, HL-60. DNA fragmentation in apoptotic cells were determined by propidium iodide (PI) staining (sub-G1 peak).
Results: Scutellaria luteo-coerulea inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. luteo-coerulea, the CH2Cl2 fraction was found to be the most cytotoxic one among others. Sub-G1 peak in flow cytometry histogram of treated cells suggested the induction of apoptosis in S. luteo-coerulea.
Conclusion: Scutellaria luteo-coerulea could be a novel candidate for further analytical elucidation in respect to fine major components responsible for the cytotoxic effect of the plant also clinical evaluations.


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Kim KW, Ha KT, Park CS, Jin UH, Chang HW, Lee IS, Kim CH. 2007. Polygonumcuspidatum, compared with baicalin and berberine, inhibits inducible nitric oxide synthase and cyclooxygenase-2 gene expressions in RAW 264.7 macrophages. Vascul Pharmacol, 47: 99-107.

Lamer-Zarawska E, Wiśniewska A, Błach-Olszewska Z. 2010. Anticancer properties of Scutellaria baicalensis root in aspect of innate immunity regulation. Adv Clin Exp Med, 19 : 419-428

Li-Weber M. 2008. New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents wogonin, baicalein and baicalin. Cancer Treat Rev, 35: 57-68.

Ma Z, Otsuyama K, Liu S, Abroun S, Ishikawa H, Tsuyama N, Obata M, Li FJ, Zheng X, Maki Y, Miyamoto K, Kawano MM. 2005. Baicalein, a component of Scutellaria radix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells. Blood, 105: 3312-8.

Malich G, Markovic B, Winder C. 1997. The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Toxicology, 124: 179-192.

Mamadalieva N, Herrmann F, El-Readib M, Tahrani A, Hamoud R, Egamberdieva D, Azimova S, Wink M. 2011. Flavonoids in Scutellaria immaculate and S. ramosissima (Lamiaceae) and their biological activity. J Pharm Pharmacol, 63: 1346-1357.

Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods,139: 271-279.

Salini S, Chubicka T, Sasidharan N, Sindhu ER, Babu TD. 2013. Cytotoxic and antioxidant properties of selected Scutellaria species from the Western Ghats of Peninsular India. Pharm Biol, 51: 152-159.

Shang X, He X, He X, Li M, Zhang R, Fan P, Zhang Q, Jia Z. 2010. The genus Scutellaria an ethnopharmacological and phytochemical review. J Ethnopharmacol, 128: 279-313.

Sonoda M, Nishiyama T, Matsukawa Y, Moriyasu M. 2004. Cytotoxic activities of flavonoids from two Scutellaria plants in Chinese medicine. J Ethnopharmacol, 91: 65-68.

Tayarani-Najaran Z, Mousavi SH, Asili J, Emami SA. 2010. Growth inhibitory effect of Scutellaria lindbergii in human cancer cell lines. Food Chem Toxicol, 48: 599-604.

Tayarani-Najaran Z, Mousavi SH, Tajfard F, Asili J, Soltani S, Hatamipour M, Emami SA. 2013. Cytotoxic and apoptogenic properties of three isolated diterpenoids from Salvia chorassanica through bioassay-guided fractionation. Food Chem Toxicol, 57: 346-51.

Tayarani-Najarani Z, Asili J, Parsaee H, Mousavi SH, Mashhadian NV, Mirzaee A, Emami SA. 2012. Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells. Rev Braz Farmacogn, 22: 268-276

Wang CZ, Li XL, Wang QF, Mehendale SR, Yuan CS. 2010. Selective fraction of Scutellaria baicalensis and its chemo preventive effects on MCF-7 human breast cancer cells. Phytomedicine, 17: 63-8.

Ye F, Xui L, Yi J, Zhang W, Zhang DY. 2002. Anticancer activity of Scutellaria baicalensis and its potential mechanism. J Altern Complement Med, 8: 567-572.

Yu J, Liu H, Lei J, Tan W, Hu X, Zou G. 2007. Antitumor activity of chloroform fraction of Scutellaria barbata and its active constituents. Phytother Res, 21: 817-22.