Anticonvulsant and neuroprotective effects of Rosa damascena hydro-alcoholic extract on rat hippocampus

Document Type: Original Research Article

Authors

1 Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective: Previously, analgesic, hypnotic, and anticonvulsant effects have been suggested for Rosa damascena (R. damascena). In the present study, possible anti-seizure and neuro-protective effects of hydro-alcoholic extract of R. damascena has been investigated after inducing seizures in rats by pentylenetetrazole (PTZ).
Materials and Methods: The rats were divided to five groups: (1) Control: received saline, (2) PTZ: 100 mg/kg, i.p., (3) PTZ-Extract 50 mg/kg(PTZ-Ext 50), (4) PTZ- Extract 100 mg/kg(PTZ-Ext 100), and (5) PTZ- Extract 200 mg/kg(PTZ-Ext 200) groups which were treated with 50, 100, and 200 mg/kg respectively of hydro-alcoholic extract of R. damascena for one week before PTZ injection. The animals were examined for electrocorticography (ECoG) recording and finally, the brains were removed for histological study.
Results: The hydro-alcoholic extract of R. damascena significantly prolonged the latency of seizure attacks and reduced the frequency and amplitude of epileptiform burst discharges induced by PTZ injection. Moreover, all three doses of the extract significantly inhibited production of dark neurons in different regions of the hippocampus in the mentioned animal model.
Conclusion: The present study showed that the hydro-alcoholic extract of R. damascena has anticonvulsant and neuroprotective effects. More investigations are needed to be done in order to better understand the responsible compound(s) as well as the possible mechanism(s).

Keywords

Main Subjects


Ankarcrona M, Zhivotovsky B, Holmström T, Diana A, Eriksson JE, Orrenius S, Nicotera P1996. "Lamin and [beta]-tubulin fragmentation precede chromatin degradation in glutamate-induced neuronal apoptosis." Neuroreport, 7: 2659-2664.

Aridogan BC, Baydar H, Kaya S, Demirci M, Ozbasar D, Mumcu E. 2002. "Antimicrobial activity and chemical composition of some essential oils." Arch Pharm Res, 25: 860-864.

Q3

Arzimanoglou A, Hirsch E, Nehlig A, Castelnau P, Gressens P, Pereira de Vasconcelos A. 2002. "Epilepsy and neuroprotection: an illustrated review." Epileptic Disord, 4: 173-182.

Ashrafzadeh F, Rakhshandeh H, Mahmodi E. 2007. "Rosa damascenaoil: an adjunctive therapy for pediatric refractory seizures." Iran J Child Neurol, 1: 13-17.

Awale S, Tohda C, Tezuka Y, Miyazaki M, Kadota S. 2011. "Protective Effects of Rosa damascenaand Its Active Constituent on Abeta(25-35)-Induced Neuritic Atrophy." Evid Based Complement Alternat Med, 2011: 131042.

Baracskay P, Szepesi Z, Orbán G, Juhász G, Czurkó A. 2008. "Generalization of seizures parallels the formation of “dark” neurons in the hippocampus and pontine reticular formation after focal–cortical application of 4-aminopyridine (4-AP) in the rat." Brain Res, 1228: 217-228.

Basim E, Basim H. 2003. "Antibacterial activity of Rosa damascenaessential oil." Fitoterapia, 74: 394-396.

Biswas N, Gupta S, Das G, Kumar N, Mongre P, Haldar D, Beri S. 2001. "Evaluation of Ophthacare eye drops—a herbal formulation in the management of various ophthalmic disorders." Phytother Res, 15: 618-620.

Boskabady MH, Kiani S, Rakhshandah H. 2006. "Relaxant effects of Rosa damascenaon guinea pig tracheal chains and its possible mechanism(s)." J Ethnopharmacol, 106: 377-382.

Boskabady MH, Shafei MN, Saberi Z, Amini S. 2011. "Pharmacological effects of rosa damascena." Iran J Basic Med Sci, 14: 295-307.

Czurkó A, Nishino H. 1993. "‘Collapsed’(argyrophilic, dark) neurons in rat model of transient focal cerebral ischemia." Neurosci Lett, 162: 71-74.

Ebrahimzadeh Bideskan AR, Hosseini M, Mohammadpour T, Karami R, Khodamoradi M, Nemati Karimooy H, Alavi H. 2011. "Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats." Zhong Xi Yi Jie He Xue Bao, 9: 611-618.

Ernst E, Barnes J, Abbot NC, Armstrong NC, Harkness E, Pittler MH, Stevinson C, Vogler B, White AR. 1998. "The Di Bella story: emotion as opposed to fact." Focus Alternative Compl Ther, 3: 143-143.

Fisher RS, van Emde Boas W, Blume W, Elger C, Genton P, Lee P, Engel J Jr. 2005. "Epileptic seizures and epilepsy: definitions proposed by the International League Against Epilepsy (ILAE) and the International Bureau for Epilepsy (IBE)." Epilepsia, 46: 470-472.

Friedman WJ. 2010. "Proneurotrophins, seizures, and neuronal apoptosis." Neuroscientist, 16: 244-252.

Gallyas F, Farkas O, Mazlo M. 2004. "Gel-to-gel phase transition may occur in mammalian cells: Mechanism of formation of "dark" (compacted) neurons." Biol Cell, 96: 313-324.

Gholamhoseinian A, Fallah H. 2009. “Inhibitory effect of methanol extract of Rosa damascenaMill. flowers on α-glucosidase activity and postprandial hyperglycemia in normal and diabetic rats" Phytomedicine, 16: 935-941.

Gorji A. 2003. "Pharmacological treatment of headache using traditional Persian medicine." Trends Pharmacol Sci, 24: 331-334.

Hajhashemi V, Ghannadi A, Hajiloo M. 2010. "Analgesic and Anti-inflammatory Effects of Rosa damascenaHydroalcoholic Extract and its Essential Oil in Animal Models." Iran J Pharm Res, 9: 163-168.

Hosseini M, Ghasemzadeh Rahbardar M, Sadeghnia HR, Rakhshandeh H. 2011. "Effects of different extracts of Rosa damascenaon pentylenetetrazol-induced seizures in mice." Zhong Xi Yi Jie He Xue Bao, 9: 1118-1124.

Hosseini M, Harandizadeh F, Niazamand S, Soukhtanloo M, Mahmoudabady M. 2013. “Antioxidant effect of Achillea wilhelmsii extract on pentylenetetrazole (seizure model)-induced oxidative brain damage in Wistar rats. "Indian J Physiol Pharmacol, 57:418-24.

Hosseini M, Harandizadeh F, Niazmand S, Soukhtanloo M, Faizpour A, Ghasem abady M. 2014. "The role for nitric oxide on the effects of hydroalcoholic extract of Achillea wilhelmsii on seizure." Avicenna J Phytomed, 4: 251-259.

Hosseini M, Jafarianheris T, Seddighi N, Parvaneh M, Ghorbani A, Sadeghnia HR, Rakhshandeh H. 2012. "Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice." Zhong Xi Yi Jie He Xue Bao 10: 1476-1481.

Hosseini M, Sadeghnia HR, Salehabadi S, Soukhtanloo M. 2013. "Contribution of estradiol in sex-dependent differences of pentylenetetrazole-induced seizures in rats." Acta Physiol Hung, 100: 237-245.

Ishida K, Shimizu H, Hida H, Urakawa S, Ida K, Nishino H. 2004. "Argyrophilic dark neurons represent various states of neuronal damage in brain insults: some come to die and others survive." Neuroscience, 125: 633-644.

Jafarian M, Rahimi S, Behnam F, Hosseini M, Haghir H, Sadeghzadeh B, Gorji A. 2010. "The effect of repetitive spreading depression on neuronal damage in juvenile rat brain." Neuroscience, 169: 388-394.

Jefferys JG. 2003. "Models and mechanisms of experimental epilepsies." Epilepsia, 44 Suppl 12: 44-50.

Karimzadeh F, Hosseini M, Mangeng D, Alavi H, Hassanzadeh GR, Bayat M, Jafarian M, Kazemi H, Gorji A. 2012. "Anticonvulsant and neuroprotective effects of Pimpinella anisum in rat brain." BMC complement altern med, 12: 76.

Kellermayer R, Zsombok A, Auer T, Gallyas F. 2006. "Electrically induced gel-to-gel phase-transition in neurons." Cell Biol Int, 30: 175-182.

Kheirabadi M, Moghimi A, Rakhshande H, Rassouli MB. 2008. "Evaluation of the anticonvulsant activities of Rosa damascenaon the PTZ induced seizures in wistar rats." J Biol Sci, 8: 426-430.

Kherani ZS, Auer RN. 2008. "Pharmacologic analysis of the mechanism of dark neuron production in cerebral cortex." Acta Neuropathol, 116: 447-452.

Kohl Z, Uyanik G, Lurding R, Schuierer G, Bogdahn U, Schroder M, Weidner N. 2011. "Selective bilateral hippocampal lesions after theophylline-induced status epilepticus causes a permanent amnesic syndrome." J Clin Neurosci, 18: 964-966.

Krumholz A, Wiebe S, Gronseth G, Shinnar S, Levisohn P, Ting T, Hopp J, Shafer P, Morris H, Seiden L, Barkley G, French J; Quality Standards Subcommittee of the American Academy of Neurology; American Epilepsy Society. 2007. "Practice Parameter: evaluating an apparent unprovoked first seizure in adults (an evidence-based review): report of the Quality Standards Subcommittee of the American Academy of Neurology and the American Epilepsy Society." Neurology, 69: 1996-2007.

Kumar N, Bhandari P, Singh B, Bari SS. 2009. "Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species Rosa damascena, Rosa bourboniana and Rosa brunonii." Food Chem Toxicol, 47: 361-367.

Libster M. 2002. "Delmar's integrative herb guide for nurses." Published October 23rd 2001 by Delmar Thomson Learning.

Loghmani-Khouzani H, Sabzi Fini O, Safari J. 2007. "Essential oil composition of Rosa damascena Mill cultivated in central Iran." Scientia Iranica, 14: 316-319.

Mahmood N, Piacente S, Pizza C, Burke A, Khan AI, Hay AJ. 1996. "The Anti-HIV Activity and Mechanisms of Action of Pure Compounds Isolated from Rosa damascena.” Biochem Biophys Res Commun, 229: 73-79.

Mohammadpour T, Hosseini M, Karami R, Sadeghnia HR, Ebrahimzadeh Bideskan AR, Enayatfard L. 2012. "Estrogen-dependent effect of soy extract on pentylenetetrazole-induced seizures in rats." Zhong Xi Yi Jie He Xue Bao, 10: 1470-1476.

Mohammadpour T, Hosseini M, Naderi A, Karami R, Sadeghnia HR, Soukhtanloo M, Vafaee F. 2014. "Protection against brain tissues oxidative damage as a possible mechanism for the beneficial effects of Rosa damascena hydroalcoholic extract on scopolamine induced memory impairment in rats." Nutr Neurosci, [Epub ahead of print]

Mansouri S, lale Ataei M, Hosseini M, Ebrahimzadeh Bideskan AR. 2013. “Tamoxifen mimics the effects of endogenous ovarian hormones on repeated seizures induced by pentylenetetrazole in rats.” Exp Neurobiol, 22:116-123.

Nascimento GG, Locatelli J, Freitas PC, Silva GL. 2000. "Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria." Braz J Microbiol, 31: 247-256.

Nyeem M, Alam M, Awal M, Mostofa M, Uddin S, Islam N, ROUF R. 2007. "CNS depressant effect of the crude ethanolic extract of the flowering tops of Rosa Damascena." Iran J Pharm Therap, 5:171-174.

Ooigawa H, Nawashiro H, Fukui S, Otani N, Osumi A, Toyooka T, Shima K. 2006. "The fate of Nissl-stained dark neurons following traumatic brain injury in rats: difference between neocortex and hippocampus regarding survival rate." Acta Neuropathol, 112: 471-481.

Poirier JL, Capek R, De Koninck Y. 2000. "Differential progression of Dark Neuron and Fluoro-Jade labelling in the rat hippocampus following pilocarpine-induced status epilepticus." Neuroscience, 97: 59-68.

Rakhshandah H, Boskabady M, Mousavi Z, Gholami maliheh SZ. 2010. "The Differences in the relaxant effects of different fractions of Rosa damascenaon guinea pig tracheal smooth muscle." Iran J Basic Med Sci, 13: 126-132

Rakhshandah H, Hosseini M. 2006. "Potentiation of pentobarbital hypnosis by Rosa damascena in mice." Indian J Exp Biol, 44: 910-912.

Rakhshandah H, Shakeri MT, Ghasemzadeh MR. 2010. "Comparative hypnotic effect of Rosa damascenafractions and Diazepam in Mice." Iran J Pharm Res, 6: 193-197.

Rakotonirina VS, Bum EN, Rakotonirina A, Bopelet M. 2001. "Sedative properties of the decoction of the rhizome of Cyperus articulatus." Fitoterapia, 72: 22-29.

Ramezani R, Moghimi A, Rakhshandeh H, Ejtehadi H, Kheirabadi M. 2008. "The effect of Rosa damascenaessential oil on the amygdala electrical kindling seizures in rat." Pakistan J biol sci, 11: 746-751.

Represa A, Niquet J, Pollard H, Ben-Ari Y. 1995. "Cell death, gliosis, and synaptic remodeling in the hippocampus of epileptic rats." J Neurobiol, 26: 413-425.

Sadeghian H, Jafarian M, Karimzadeh F, Kafami L, Kazemi H, Coulon P, Ghabaee M, Gorji A2012. "Neuronal death by repetitive cortical spreading depression in juvenile rat brain." Exp Neurol, 233: 438-446.

Senol F, Orhan I, Kürkçüoğlu M, Khan M, Altintas A, Şener B, Baser KHC. 2011. "An in vitro approach to neuroprotective activity of Rosa damascena Mill., a medieval age traditional medicine used for memory enhancement." Planta Medica, 77: PM163.

Shafei MN, Rakhshandah H, Boskabady MH. 2003. "Antitussive effect of Rosa damascenain guinea pigs." Iran J Pharm Res, 231-234.

Toth Z, Yan XX, Haftoglou S, Ribak CE, Baram TZ. 1998. "Seizure-induced neuronal injury: vulnerability to febrile seizures in an immature rat model. J Neurosci, 18:4285-4294.

Ulusoy S, Boşgelmez-Tınaz G, Seçilmiş-Canbay H. 2009. "Tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute." Curr Microbiol, 59: 554-558.

Wie M-B, Won M-H, Lee K-H, Shin J-H, Lee J-C, Suh H-W, Song DK, Kim YH. 1997. "Eugenol protects neuronal cells from excitotoxic and oxidative injury in primary cortical cultures." Neurosci Lett, 225: 93-96.

Yassa N, Masoomi F, Rankouhi SR, Hadjiakhoondi A. 2009. "Chemical composition and antioxidant activity of the extract and essential oil of Rosa damascena from Iran, population of Guilan." DARU J Pharm Sci, 17: 175-180.

Zargari A. 1992. Medicinal plants, vol. 2, Tehran University Press, Tehran.

Zsombok A, Tóth Z, Gallyas F. 2005. "Basophilia, acidophilia and argyrophilia of “dark”(compacted) neurons during their formation, recovery or death in an otherwise undamaged environment." J Neurosci Methods, 142: 145-152