Antidiarrheal activity and acute oral toxicity of Mentha longifolia L. essential oil

Document Type: Original Research Article

Authors

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Objectives: Mentha longifolia L. (Lamiaceae) is an annual herb that is used in the Iranian traditional medicine for treating stomach and intestinal disorders. The purpose of this study was to determine the protective effect of M. longifolia on experimental diarrhea in a rat model.
Materials and Methods: The antidiarrheal activity of essential oil of M. longifolia (20-80 mg/kg) was investigated against castor oil-induced diarrhea in rats using loperamide as the standard reference drug. In acute toxicity evaluation, rats were orally administrated with single dose of EOML at doses ranging from 10 to 1000 mg/kg.
Results: EOML caused a significant (p<0.05) and dose-dependent decrease of gastrointestinal transit, nevertheless, it could not block the inhibitory effect of atropine (0.1 mg/kg). EOML at oral doses of 20 and 80 mg/kg protected the animals against castor oil-induced diarrhea significantly (p<0.05). EOML decreased the intestinal fluid accumulation as indicated by the significantly (pp<0.001) decrease compared to control. The oral LD50 of EOML was found to be 470 mg/kg in rat.
Conclusion: Since the inhibition of intestinal hyperactivity and hypersecretory are the bases of the treatment of diarrhea, results obtained in the present study suggest that EOML is endowed with antidiarrheal activity. EOML is moderately toxic for oral medication

Keywords


Berthelsen S, Pettinger WA. 1977. A functional basis for the classification of a2 adrenergic receptors. Life Sci, 21: 595–606.

Brankovic SV, Kitic DV, Radenkovic MM, Veljkovic SM, Golubovic TD. 2009. Calcium blocking activity as a mechanism of the spasmolytic effect of the essential oil of Calamintha glandulosa Silic on the isolated rat ileum. Gen Physio Biophys, 28: 74–178.

Coelho-de-Souza AN, Barata EL, Magalhaes PJC, Lima CC, Leal-Cardoso JH. 1997. Effects of the essential oil of Croton zehntneri, and its constituent estragole on intestinal smooth muscle. Phytother Res,11: 299-304.

De Urbina AVO, Martin MLM, Montero J, Carron R, San Roman L. 1990. Antihistiminic activity of pulegone on the guinea-pig ileum. J Pharm Pharmacol, 42: 295-296.

Gilani GH, Rahman A. 2005. Trends in ethnopharmacology. J Ethnopharmacol, 100:43–49.

Guimaraes AG, Quintans JS, Quintans LJJr. 2013. Monoterpenes with analgesic activity – a systematic review. Phytother Res,27: 1-15.

Gurgel LA, Silva RM, Santos FA, Martins DTO, Mattos PO, Rao VSN. 2001. Studies on the antidiarrheal effect of dragon’s blood from Croton urucarana. Phytother Res,15: 319–322.

Gutierrez SP, Sanchez MAZ, Gonzalez CP, Garcia LA. 2007. Antidiarrheal activity of different plants used in traditional medicine. Afr J Biotechnol, 6: 2988-2994.

Hsu WH. 1982. Xylazine-induced delay in small intestine transit in mice. Eur J Pharmacol,83: 55–60.

Jalilzadeh-Amin G, Maham M, Dalir-Naghadeh B, Kheiri F. 2012. Effects of Mentha longifolia essential oil on ruminal and abomasal longitudinal smooth muscle in sheep. J Essent Oil Res,24: 61–69.

Jalilzadeh-Amin G, Maham M. The application of 1,8-cineole, a terpenoid oxide present in medicinal plants, inhibits castor oil-induced diarrhea in rats. Pharm Biol, DOI:10.3109/13880209.2014.935862.

Jalilzadeh-Amin G, Mahama M. 2013. Evaluation of pulegone on transit time and castor-oil induced diarrhea in rat. Pharmaceutical Sci,19: 77-82.

Kawata J, Kameda M, Miyazawa M. 2008. Cyclooxygenase-2 inhibitory effects of monoterpenoids with a p-menthane skeleton. Int J Essen Oil Therapeut,2: 145-148.

Leal-Cardoso JH, Matos-Brito BG, Lopes-Junior JE, Viana-Cardoso KV, Sampaio-Freitas AB, Brasil RO, Coelho-De-Souza AN, Albuquerque AA. 2004. Effects of estragole on the compound action potential of the rat sciatic nerve. Braz J Med Biol Res,37, 1193-1198.

Magalhaes PJC, Criddle DN, Tavares RA, Melo EM, Mota TL, Leal-Cardoso JH. 1998. Intestinal myorelaxant and anti-spasmodic effects of the essential oil of Croton nepetaefolius, and its constituents cineole, methyl-eugenol and terpineol. Phytother Res, 12: 172–177.

Martini N, Eloff JN. 1998. The preliminary isolation of several antibacterial compounds from Combretum erytrophyllum (Combretaceae). J Ethnopharmacol,62: 255–263.

Meyrowitsch DW, Bygbjerg CI. 2007. Global burden of disease - a race against time. Dan Med Bull, 54:32-34.

Moorthy B. 1991. Toxicity and metabolism of R-(+)-pulegone in rats: its effects on hepatic cytochrome P450 in vivo and in vitro. J Indian Inst Sci, 71: 76-78. 

Nelson SD. 1995. Mechanisms of the formation and disposition of reactive metabolites that can cause acute liver injury. Drug Metab Rev,27:147–177.

Odeyemi OO, Yakubu MT, Masika PJ, Afolayan AJ. 2009. Toxicological Evaluation of the Essential Oil from Mentha longifolia L. subsp. capensis Leaves in Rats. J Med Food, 12: 669-674.

Ouyan H, Chen DH. 2004. Review on therapeutic roles of acupuncture in functional gastrointestinal disorders. Aliment Pharmacol Ther,20: 831–841.

Palombo EA. 2006. Phytochemicals from traditional medicinal plants used in the treatment of diarrhea: modes of action and effects on intestinal function. Phytother Res, 20: 717–724.

Perez Raya MD, Utrilla MP, Navarro MC, Jiminez J. 1990. CNS activity of Mentha rotundifolia and Mentha longifolia essential oil in mice and rats. Phytother Res, 4: 232-234.

Rates SMK. 2001. Plants as source of drugs. Toxicon,39: 603–613.

Santos FA, Rao VSN. 2000. Anti-inflammatory and antinociceptive effects of 1,8-cineole, a terpenoid oxide present in many plant essential oils. Phytother Res,13: 1–5.

Shabana, C, Ahsana D, Shakeel A, Atta-ur-Ralman. 2005. Evaluation of Alstonia scholaris leaves for broncho-vasodilatatory activity. J Ethnopharmacol, 97: 469–476.

Shah AJ, Bhulani NN, Khan SH, Rehman N, Gilani AH. 2010. Calcium channel blocking activity of Mentha longifolia L. explains its medicinal use in diarrhea and gut spasm. Phytother Res, 24: 1392–1397.

Slama K. 1978. The principles of antihormone action in insects. Acta Entomol Bohemoslov,75: 65–82.

Soares PM, Assreuy AM, Souza EP, Lima RF, Silva TO, Fontenele SR, Criddle DN. 2005. Inhibitory effects of the essential oil of Mentha pulegium on the isolated rat myometrium. Planta Med,71: 214–218.

Thomassen D, Slattery JT, Nelson SD. 1990. Menthofuran-dependent and independent aspects of pulegone hepatotoxicity: roles of glutathione. J Pharmacol Exp Ther, 253: 567-572.

Zargari A. 1990. Medicinal Plants, (4nd edn), pp. 14-18, Tehran, Tehran University Publications.