Anti-inflammatory, Analgesic and Antiulcer properties of Porphyra vietnamensis

Document Type : Original Research Article

Authors

1 DM College of Pharmacy, Bahadurgah, Haryana, India.

2 Deptt. of Pharmaceutical Sciences, Jamia Hamdard, New Delhi, India.

3 Seoul National University, Republic of Korea, Korea.

4 Division of Pharmaceutics, Faculty of Medical Sciences, Lovely Professional University, Phagwara, Punjab India.

5 Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.

Abstract

Objectives: Aim of the present work was to investigate the anti-inflammatory, analgesic and antiulcer effects of red seaweed Porphyra vietnamensis (P. vietnamenis).
Materials and Methods: Aqueous (POR) and alcoholic (PE) fractions were successfully isolated from P. vietnamenis. Further biological investigations were performed using a classic test of paw edema induced by carrageenan, writhing induced by acetic acid, hot plate method and naproxen induced gastro-duodenal ulcer.
Results: Among the fractions POR showed better activity.  POR and PE significantly (p < 0.05) reduced carrageenan induced paw edema in a dose dependent manner. In the writhing test POR significantly (p < 0.05) reduced abdominal writhes than PE.  In hot plate method POR showed better analgesic activity than PE. POR showed comparable ulcers reducing potential (p<0.01) to that of omeprazole, and has more ulcer reducing potential then PE.
Conclusions: The results of this study demonstrated that P. vietnamenis aqueous fraction possesses biological activity that is close to the standards taken for the treatment of peripheral painful or/and inflammatory and ulcer conditions.

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Main Subjects


Bandyopadhyay U, Biswas K, Chatterjee R, Bandyopadhyay D, Chattopadhyay I, Ganguly CK, Chakraborty T, Bhattacharya K, Banerjee RK. 2002. Gastroprotective effect of Neem (Azadirachta indica) bark extract: Possible involvement of H+-K+-ATPase inhibition and scavenging of hydroxyl radical. Life Sciences, 71: 2845–2865.
Bhatia S, Namdeo AG, Nanda S. 2010b. Factors effecting the gelling and emulsifying properties of a natural polymer.Syst Rev Pharm, 1: 86-92.
Bhatia S, Garg A, Sharma K, Kumar S,Sharma A, PurohitAP. 2011. Mycosporine and mycosporine-like amino acids: A paramount tool against ultra violet irradiation. Pharmacog Rev, 5: 138–146.
Bhatia S, Rathee P, Sharma K, Chaugule BB, Kar N, Bera T. 2013. Immuno-modulation effect of sulphated polysaccharide (porphyran) from Porphyra vietnamensis. Int J Bio Macromol, 57: 50-56.
 Bhatia S, Sharma A, Sharma K, Kavale M, Chaugule BB, Dhalwal K, NamdeoAjay G, Mahadik KR. 2008. Novel Algal Polysaccharides from Marine Source: Porphyran. Pharmacog Rev, 2: 271-276.
Bhatia S, Sharma K, Namdeo AG, Chaugule BB, Kavale M, Nanda S. 2010a. Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis. Pharmacog Res, 2: 45-49.
Carté, BK. 1996. Biomedical Potential of Marine Natural Products. Biosciences, 271-286. De Vries DJ, Beart PM. 1995. Fishing for Drugs from the Sea: Status and Strategies. TIPS, 16: 275-279.
Dirk K, Bernhard K, Christof IK, Peter HK, Wolfgang S, Jochen R. 2001. Vacuolating Cytotoxin of Helicobacter pylori Induces Apoptosis in the Human Gastric Epithelial Cell Line AGS. Infect Immun, 69: 5080–5087.
Dhargalkar VK, Pereira N. 2005. Seaweed: promising plant of the millennium. Sci Culture, 4: 60–66.
Dirk K, Bernhard K, Christof IK, Peter HK, Wolfgang S, Jochen R. 2001. Vacuolating Cytotoxin of Helicobacter pylori Induces Apoptosis in the Human Gastric Epithelial Cell Line AGS. Infect Immun, 69: 5080–5087.
Faulkner DJ. 1995. Chemical Riches from the Ocean. Chem Brit, 2: 680-684.
Gonzalez R, Rodriguez S, Romay C, Ancheta O, Gonzalez A, Armesta J,Remirez D, Merino N. 1999. Anti-inflammatory activity of phycocyanin extract in acetic acid-induced colitis in rats. Pharmacol Res, 39: 55–59.
Guillen MEN, Emim JADS, Souccar C, Lopa AJ. 1997. Analgesic and Anti-inflammatory activities of aqueous extract of plantago major L. Int.JPharmacog.,35(2): 99-104.
Haefner, B. 2003. Drugs from the deep: marine natural products as drug candidates. Drug Discov Today,8: 536–544.
Kang JY, Khan MNA, Park NH, Cho JY, Lee MC, Fujii H, Hong YK. 2008. Antipyretic, analgesic and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacol, 116: 187–190.
Khan MNA, Choi JS, Lee MC, Kim E, Nam TJ, Fujii H, Hong YK.  2008. Antiinflammatory activities of methanol extracts from various seaweed species. J Envir Biol, 29: 465–469.
Kim J, Kimb SY, Songe G, Parkd J and Changa H. 2005. Protective effect of astaxanthin on naproxen induced gastric antral ulceration in rats. Eur J Pharmacol, 514: 53-59.
Lanhers MC, Fleurentin J, Dorfman P, Motrier F, Pelt JM. 1991. Analgesic anti-inflammatory and antipyretic properties of Euphorbia hirta. Planta Medica, 57: 225-231.
Lee BB, Choi JS, Moon HE, Ha YM, Kim MS, Cho KK, Choi IS. 2013. Inhibition of growth and urease of Helicobacter pylori by Korean edible seaweed extracts. Botanical Sci, 91: 515-522.
Mayer AMS, Lehmann VKB. 2000. Marine Pharmacology in 1998: Marine Compounds with Antibacterial, Anticoagulant, Anti-inflammatory, Anthelmintic, Antiplatelet, Antiprotozoal, and Antiviral Activities; with actions on the Cardiovascular, Endocrine, Immune, and Nervous Systems; and other Miscellaneous Mechanisms of Action The Pharmacologist, 42: 62-69.
N Yoshida, N Sugimoto, F Hirayama, Y Nakamura, H Ichikawa, Y Naito, T Yoshikawa. 2002. Helicobacter pylori infection potentiates aspirin induced gastric mucosal injury in Mongolian gerbils. Gut, 50: 594–598.
Pandit V, Suresh S, Joshi H. 2008. Peptic ulcer and its management. Journal of Pharmacy Res, 1: 245-252.
Qiao WL, Wang GM, Shi Y, Wu JX, Qi YJ, Zhang JF, Sun H, Yan CD. 2011. Differential expression of Bcl-2 and Bax during gastric ischemia-reperfusion of rats. World J Gastroenterol, 17: 1718–1724.
Rinehart KL, Kishore V, Bible KC, Sakai R, Sullins DW, Li KM. 1988. Didemnins and Tunichlorin: Novel Natural Products from the Marine Tunicate Trididemnum solidum. J. Nat Prod, 51: 1-21.
Shu MH, Appleton D, Zandi K, AbuBakar S. 2013. Anti-inflammatory, gastroprotective and anti-ulcerogenic effects of red algae Gracilaria changii (Gracilariales, Rhodophyta) extract. BMC Complementary and Alternative Medicine, 13: 61.  
Smit AJ. 2004. Medicinal and pharmaceutical uses of seaweed natural products: a review. J Applied Phycol, 16:245–262.
Sonnenberg A. 1994. Peptic ulcer. In Digestive diseases in the United States:epidemiology and impact. Edited by Everhart JE, pp. 357–408, Washington, DC: National Institutes of Health Publication.
Suzuki H, N Toshihiro, Tsugawa H, M Sachiko, Hibi T. 2012. Roles of oxidative stress in stomach disorders. J Clin Biochem Nutr, 50: 35–39.
Slomiany BL, Piotrowski J, Slomiany A. 1997. Induction of Tumor Necrosis Factor-α and Apoptosis in Gastric Mucosal Injury by Indomethacin: Effect of Omeprazole and Ebrotidine. Scand J Gastroenterol, 32: 638-642.
Tripathi KD. 2003. Drugs for peptic ulcer. In Essentials of Medical Pharmacology. 5th edition, pp. 587–598, New Delhi, Jaypee Brothers Medical Publishers (P) Ltd.
Wakefield D, Kumar RK. 2001. Inflammation: chronic. In Encyclopedia of lifesciences (ELS). London, Nature Publishing Group.
Whittle, BA. 1964. The use of change in capillary permeability to distinguish between narcotic and analgesic. British J. Pharmacol. Chemother 22(2): 246-253.
Winter CA, Risley EA, Nuss CW. 1962. Carrageenan induced oedema in hind paw of rats as an assay for anti-inflammatory drugs. In: Proc. Society Exp Boil Med, 11: 544-547.
Woolfe G, MacDonald AD. 1994. The evaluation of the analgesic action of Pethidine Hydrochloride. J Pharmacol Exp Ther, 80: 300.