1Graduate Student in Animal Sciences, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, I. R. Iran
2Department of Animal Sciences, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, I. R. Iran
3Department of Pathology, College of Veterinary Medicine, Ferdowsi of University of Mashhad, Mashhad, I. R. Iran
Objectives: Hypericum perforatum L. is a medicinal plant containing many polyphenolic compounds, including flavonoids and phenolic acids with antidepressant and anti-inflammatory properties. This study was investigated the effects of Hypericum perforatum extract (HPE) on immunity, body weight (BW), and spleen index (SI) in rats. Materials and Methods: A total of 24 Wistar male rats were randomly received 4 different doses (6 rats each) of HPE (0, 100, 200 and 400 mg/kg BW) intraperitoneally for 14 days using a completely randomized design. On days 1 and 7, rats were received 0.5 ml SRBC (10%) injection. Blood samples were collected on day 14 to evaluate IgG titer and leukocyte count. On days 1, 7 and 14, the BW and on day 14 spleen were weighted for SI. Results: The IgG titer increased with higher doses of HPE. The HPE increased number of lymphocytes at 200 mg but decreased at 400 mg, number of neutrophils decreased at 200 mg but increased at 400 mg, and number of monocytes increased at 100 mg and 200 mg but decreased at 400 mg (p<0.01). Increasing doses of HPE lowered BW (p<0.01). The HPE increased SI at 100 mg and 200 mg but decreased at 400 mg (p>0.072). Conclusions: The results showed that HPE slightly improved IgG titer but significantly increased the number of leukocytes and monocytes at 200 mg, and neutrophils at 400 mg. The HPE decreased BW at 100 mg and 200 mg with no damage on spleen.
Agrawal SS, khadase SC, Talele GS. 2010. Studies on immunomodulatory activity of Capparis zeylanica leaf extracts. Int J Pharm Sci Nanotech, 3: 887-892.
Andrew V, Catherine Z. 1999. ABC of complementary medicine: Herbal Med J, 319: 1050-1053.
Bafor EE, Igbinuwen O. 2009. Acute toxicity studies of the leaf extract of Ficus exasperata on haematological parameters, body weight and body temperature. J Ethnopharmacol, 123: 302-307.
Bickerdike MJ. 2003. 5-HT2C receptor agonists as potential drugs for the treatment of obesity. Curr Top Med Chem, 3: 885-897.
Bombardelli E, Morazzoni P. 1995. Hypericum perforatum. Fitoterapia, 66: 43-68.
Can-Özgür D, Özkay-Demir Ü, Öztürk N, Öztürk Y. 2008. Hypophagic effect of Hypericum perforatum L. on rats. Federation Asian Pharmace Asso Cong (FAPA), Singapore, Proceedings and Abstracts, SCP0, 26, p.424.
Chatterjee SS, Bhattacharya SK, Wonnemann M, Singer A, Muller WE. 1998a. Hyperforin as a possible antidepressant component of Hypericum extracts. Life Sci, 63: 499-510.
Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. 2001. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev, 53: 135-159.
Dalton GL, Lee MD, Kennett GA, Dourish CT, Clifton PG. 2006. Serotonin 1B and 2C receptor interactions in the modulation of feeding behaviour in the mouse. Psychopharm (Berl), 185: 45-57.
Fitzpatrick FK. 1954. Plant substances active against Mycobacterium tuberculosis. Antibio Chemother, 4: 528-536.
Evstifeeva TA, Sibiriak SV. 1996. The immunotropic properties of biologically active products obtained from Klamath weed (Hypericum perforatum L.). Eksp Klin Farmakol, 59: 51-54.
Hirano K, Kato Y, Uchida S, Sugimoto Y, Yamada J, Umegaki K, Yamada S. 2004. Effects of oral administration of extracts of Hypericum perforatum (St John's wort) on brain serotonin transporter, serotonin uptake and behaviour in mice. J Pharm Pharmacol, 56: 1589-1595.
Huang N, Singh N, Yoon K, Loiacono CM, Kohut ML, Birt, DF. 2013. The immuno-regulatory impact of orally-administered Hypericumperforatum extract on Balb/C mice inoculated with H1n1 influenza A virus. PLoS ONE 8: e76491.
Jiang W, Liu Y, Zheng H, Zheng Y, Xu H, Lu H. 2012. Immune regulation of avian influenza vaccine in hens using Hypericum perforatum L. methanol extraction. PlantOmics J, 5: 40-45.
Kumar V, Singh PN, Bhattacharya SK. 2001. Anti-stress activity of Indian Hypericum perforatum L. Indian J Exp Biol, 39: 344-349.
Landy N, Ghalamkari GH Toghyani M. 2012. Evaluation of St John's Wort (Hypericum perforatum L.) as an antibiotic growth promoter substitution on performance, carcass characteristics, some of the immune responses, and serum biochemical parameters of broiler chicks. J Med Plants Res. 6: 510-515.
Liu FF, Ang CY, Heinze TM, Rankin JD, Beger RD, Freeman JP, Lay JO Jr. 2000. Evaluation of major active components in St. John's Wort dietary supplements by high-performance liquid chromatography with photodiode array detection and electrospray mass spectrometric confirmation. J Chromatogr A, 888: 85-92.
Lucas, AM, Jamroz C. 1961. Atlas of avian hematology. USDA Agriculture Monograph Gr 25. USDA, Washington DC.
Müller WE. 2003. Current St. John’s wort research from mode of action to clinical efficacy. Pharmacol Res, 47: 101-109.
Nahrstedt A, Butterweck V. 1997. Biologically active and other chemical constituents of the herb of Hypericum perforatum L. Pharmacopsychiatry, 30: 129-134.
Pirbaluti AA, Jafarian Deh Kordi M, Poor Hakim Davoodi R, Hamedi R, Rabie M. 2011. Effects of Hypericum scabrum L. on hematology, weight and body temperature of mous. J Herbal Med, 1: 25-31.
Richter A, Davies DE. 1995. Effects of anthralin and hypericin on growth factor signaling and cell proliferation in vitro. Biochem Pharma, 50: 2039-2045.