Effects of Hypericum perforatum extract on IgG titer, leukocytes subset and spleen index in rats

Document Type: Original Research Article

Authors

1 Graduate Student in Animal Sciences, College of Agriculture, Ferdowsi University of ‎Mashhad, Mashhad, I. R. Iran

2 Department of Animal Sciences, College of Agriculture, Ferdowsi University of ‎Mashhad, Mashhad, I. R. Iran

3 Department of Pathology, College of Veterinary Medicine, Ferdowsi of University of ‎Mashhad, Mashhad, I. R. Iran

Abstract

Objectives: Hypericum perforatum L. is a medicinal plant containing many polyphenolic compounds, ‎including flavonoids and phenolic acids with antidepressant and anti-inflammatory properties. ‎This study was investigated the effects of Hypericum perforatum extract (HPE) on immunity, ‎body weight (BW), and spleen index (SI) in rats.‎
Materials and Methods: A total of 24 Wistar male rats were randomly received 4 different doses (6 rats each) of HPE ‎‎(0, 100, 200 and 400 mg/kg BW) intraperitoneally for 14 days using a completely ‎randomized design. On days 1 and 7, rats were received 0.5 ml SRBC (10%) injection. Blood ‎samples were collected on day 14 to evaluate IgG titer and leukocyte count. On days 1, 7 and ‎‎14, the BW and on day 14 spleen were weighted for SI. ‎
Results: The IgG titer increased with higher doses of HPE. The HPE increased number of ‎lymphocytes at 200 mg but decreased at 400 mg, number of neutrophils decreased at 200 mg ‎but increased at 400 mg, and number of monocytes increased at 100 mg and 200 mg but ‎decreased at 400 mg (p<0.01). Increasing doses of HPE lowered BW (p<0.01). ‎The HPE increased SI at 100 mg and 200 mg but decreased at 400 mg (p>0.072).  ‎
Conclusions: The results showed that HPE slightly improved IgG titer but significantly increased ‎the number of leukocytes and monocytes at 200 mg, and neutrophils at 400 mg. The HPE ‎decreased BW at 100 mg and 200 mg with no damage on spleen.  ‎

Keywords

Main Subjects


Agrawal SS, khadase SC, Talele GS. 2010. Studies on immunomodulatory activity of  Capparis zeylanica leaf extracts. Int J Pharm Sci Nanotech, 3: 887-892.

Andrew V, Catherine Z. 1999. ABC of complementary medicine: Herbal Med J, 319: 1050-1053.

Bafor EE, Igbinuwen O. 2009. Acute toxicity studies of the leaf extract of Ficus exasperata on haematological parameters, body weight and body temperature. J Ethnopharmacol, 123: 302-307.

Bickerdike MJ. 2003. 5-HT2C receptor agonists as potential drugs for the treatment of obesity. Curr Top Med Chem, 3: 885-897.

Bombardelli E, Morazzoni P. 1995. Hypericum perforatum. Fitoterapia, 66: 43-68.

Can-Özgür D, Özkay-Demir Ü, Öztürk N, Öztürk Y. 2008. Hypophagic effect of Hypericum perforatum L. on rats. Federation Asian Pharmace Asso Cong (FAPA), Singapore, Proceedings and Abstracts, SCP0, 26, p.424.

Chatterjee SS, Bhattacharya SK, Wonnemann M, Singer A, Muller WE. 1998a. Hyperforin as a possible antidepressant component of Hypericum extracts. Life Sci, 63: 499-510.

Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. 2001. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev, 53: 135-159.

Dalton GL, Lee MD, Kennett GA, Dourish CT, Clifton PG. 2006. Serotonin 1B and 2C receptor interactions in the modulation of feeding behaviour in the mouse. Psychopharm (Berl), 185: 45-57.

Fitzpatrick FK. 1954. Plant substances active against Mycobacterium tuberculosis. Antibio Chemother, 4: 528-536.

Evstifeeva TA, Sibiriak SV. 1996. The immunotropic properties of biologically active products obtained from Klamath weed (Hypericum perforatum L.). Eksp Klin Farmakol, 59: 51-54.

Hirano K, Kato Y, Uchida S, Sugimoto Y, Yamada J, Umegaki K, Yamada S. 2004.  Effects of oral administration of extracts of Hypericum perforatum (St John's wort) on brain serotonin transporter, serotonin uptake and behaviour in mice. J Pharm Pharmacol, 56: 1589-1595.

Huang N, Singh N, Yoon K, Loiacono CM, Kohut ML, Birt, DF. 2013. The immuno-regulatory impact of orally-administered Hypericum perforatum extract on Balb/C mice inoculated with H1n1 influenza A virus. PLoS ONE 8: e76491.

Jiang W, Liu Y, Zheng H, Zheng Y, Xu H, Lu H. 2012. Immune regulation of avian influenza vaccine in hens using Hypericum perforatum L. methanol extraction. PlantOmics J, 5: 40-45.

Kumar V, Singh PN, Bhattacharya SK. 2001. Anti-stress activity of Indian Hypericum perforatum L. Indian J Exp Biol, 39: 344-349.

Landy N, Ghalamkari GH Toghyani M. 2012. Evaluation of St John's Wort (Hypericum perforatum L.) as an antibiotic growth promoter substitution on performance, carcass characteristics, some of the immune responses, and serum biochemical parameters of broiler chicks. J Med Plants Res. 6: 510-515.

Liu FFAng CYHeinze TMRankin JDBeger RDFreeman JPLay JO Jr. 2000. Evaluation of major active components in St. John's Wort dietary supplements by high-performance liquid chromatography with photodiode array detection and electrospray mass spectrometric confirmation. J Chromatogr A, 888: 85-92.

Lucas, AM, Jamroz C. 1961. Atlas of avian hematology. USDA Agriculture Monograph Gr 25. USDA, Washington DC.

Müller WE. 2003. Current St. John’s wort research from mode of action to clinical efficacy. Pharmacol Res, 47: 101-109.

Nahrstedt A, Butterweck V. 1997.  Biologically active and other chemical constituents of the herb of Hypericum perforatum L. Pharmacopsychiatry, 30: 129-134.

Pirbaluti AA, Jafarian Deh Kordi M, Poor Hakim Davoodi R, Hamedi R, Rabie M. 2011. Effects of Hypericum scabrum L. on hematology, weight and body temperature of mous. J Herbal Med, 1: 25-31.

Richter A, Davies DE. 1995. Effects of anthralin and hypericin on growth factor signaling and cell proliferation in vitro. Biochem Pharma, 50: 2039-2045.

Ruofeng SCheng HJiongran CXiuying PYu LLanying HLing WJianping L. 2012. Hypericum perforatum extract therapy for chickens experimentally infected with infectious bursal disease virus and its influence on immunity. Can J Vet Res, 76: 180-185.

Schempp CMWinghofer BMüller KSchulte-Mönting JMannel MSchöpf ESimon JC. 2003. Effect of oral administration of Hypericum perforatum extract (St. John's Wort) on skin erythema and pigmentation induced by UVB, UVA, visible light and solar simulated radiation. Phytother Res, 17: 141-6.

Vaibhav DA, Arun Kumar W. 2010. Immunomodulatory effect of alcoholic extract of terminalia chebula ripe fruits. Pharma Sci Res, 9: 539-544.

Weissleder R, Pittet MJ. 2009. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Sci, 325: 612-616. 

Wilasrusmee C, Kittur S, Siddiqui J, Bruch D, Wilasrumee S, Kittur DS. 2002. In Vitro immunomodulatory effects of ten commonly used herbs on Murine lymphocytes. J Altern Complement Med, 8: 467-475.

Xia Z, Wang G, Wan C, Liu T, Wang S, Wang B, Cheng R. 2010. Expression of NALP3 in the spleen of mice with protal hypertension. JHuazhong Univ Sci Tech Med Sci, 30: 170-172.

Xiu-ying Pu, Jian-ping Liang, Ruo-feng Shang, Xue-hong Wang, Lan-ying Hua, Yu Liu. 2009. Influence of Hypericum perforatum extract on piglet infected with porcine respiratory and reproductive syndrome virus. Agricul Sci China, 8: 730-739.