1Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
2Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
3Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, I. R. Iran
4Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes. Materials and Methods: Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls. Results: The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes. Conclusion: According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.
Biswas M, Haldar PK and Ghosh AK. 2010. Antioxidant and free-radical-scavenging effects of fruits of Dregea volubilis. J Nat Sci Biol Med, 1: 29-34.
Fazli-Bazzaz BS, Memariani Z, Khashiarmanesh Z, Iranshahi M and Naderinasab M. 2010. Effect of galbanic Acid, a sesquiterpene coumarin from ferula szowitsiana, as an inhibitor of efflux mechanism in resistant clinical isolates of Staphylococcus aureus. Braz J Microbiol, 41: 574-580.
Flora SJ. 2009. Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid Med Cell Longev, 2: 191-206.
Gholami O, Jeddi-Tehrani M, Iranshahi M, Zarnani AH and Ziai SA. 2013. Umbelliprenin from Ferula szowitsiana Activates both Intrinsic and Extrinsic Pathways of Apoptosis in Jurkat T-CLL cell line. Iran J Pharm Res, 12: 371-376.
Gholitabar S and Roshan VD . 2013. Effect of treadmill exercise and Ferula gummosa on myocardial HSP72, vascular function, and antioxidant defenses in spontaneously hypertensive rats. Clin Exp Hypertens, 35: 347-354.
Iranshahi M, Arfa P, Ramezani M, Jaafari MR. Sadeghian H, Bassarello C, Piacente S and Pizza C. 2007. Sesquiterpene coumarins from Ferula szowitsiana and in vitro antileishmanial activity of 7-prenyloxycoumarins against promastigotes. Phytochem, 68:554–561.
Ji HF, Li XJ and Zhang HY. 2009. Natural products and drug discovery. Can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia? EMBO Rep, 10: 194-200.
Kasaian J, Iranshahy M and Iranshahi M. 2013. Synthesis, biosynthesis and biological activities of galbanic acid - A review. Pharm Biol. [Epub ahead of print]
Kavoosi G and Rowshan V. 2013. Chemical composition, antioxidant and antimicrobial activities of essential oil obtained from Ferula assa-foetida oleo-gum-resin: effect of collection time. Food Chem, 138: 2180-2187.
Kavoosi G, Tafsiry A, Ebdam A and Rowshan V. 2013. Evaluation of antioxidant and antimicrobial activities of essential oils from Carum copticum seed and Ferula assafoetida latex. J Food Sci, 78: T356-361.
Lee HJ, Jeong SJ, Lee HJ, Lee EO, Kim HS, Zhang Y, Ryu SY, Lee MH, Lü J, Kim SH. 2011. Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation .Pharm Res, 28: 597-609.
Lin KH, Yang YY, Yang CM, Huang MY, Lo HF, Liu KC, Lin HS, Chao PY. 2013. Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes. BMC Res Notes 6: 490.
Lobo V, Patil A, Phatak A, Chandra N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev, 4: 118-126.
Paydar M, Wong YL, Moharam BA, Movahed E, Wong WF and Looi CY. 2013. Pharmacological Activities and Chemical Constituents of Ferula szowitsiana DC. Journal of Medical Sciences, 13: 236-243.
Riveiro ME, De Kimpe N, Moglioni A, Vázquez R, Monczor F, Shayo C and Davio C. 2010. Coumarins: old compounds with novel promising therapeutic perspectives. Curr Med Chem, 17: 1325-1338.
Shahverdi AR, Fakhimi A, Zarrini G, Dehghan G and Iranshahi M. 2007. Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biol Pharm Bull, 30: 1805-1807.
Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res, 175: 184-191.
Soltani F, Mosaffa F, Iranshahi M, Karimi G, Malekaneh M, Haghighi F and Behravan J. 2010. Auraptene from Ferula szowitsiana protects human peripheral lymphocytes against oxidative stress. Phytother Res, 24: 85-89.
Stevenson DE and Hurst RD. 2007. Polyphenolic phytochemicals just antioxidants or much more? Cell Mol Life Sci, 64: 2900-2916.
Talat gooshchi S, Dalirrad M, Bahmani M.2012. Study on chemical constituents of the roots of Ferula szowitsiana DC and evaluation of their antioxidant activity.RPS ,7: 732-738.
Uttara B, Singh AV, Zamboni P and Mahajan RT. 2009. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol, 7: 65-74.
Wu L, Wang X, Xu W, Farzaneh F, Xu R. 2009 . The structure and pharmacological functions of coumarins and their derivatives. Curr Med Chem 16(32): 4236-4260.
Yeo JD, Jeong MK, Park CU and Lee J. 2010 . Comparing antioxidant effectiveness of natural and synthetic free radical scavengers in thermally-oxidized lard using DPPH method. J Food Sci, 75: C258-262.