Moderate dose of watercress and red radish does not reduce oxygen consumption during graded exhaustive exercise

Document Type: Original Research Article

Authors

Department of Physical Education and Sports Sciences, University of Mohaghegh Ardabili, Ardabil, I. R. Iran

Abstract

Objective: Very recent studies have reported positive effects of dietary nitrate on the oxygen consumption during exercise. This research aimed to study the effect of moderate dose of high-nitrate vegetables, watercress (Nasturtium officinale) and red radish (Raphanus sativus) compared with a control group on the incremental treadmill exercise test following a standard Bruce protocol controlled by computer.
Materials and Methods: Group 1 consumed 100 g watercress (n=11, 109.5 mg nitrate/day), and group 2 consumed 100 g red radish (n=11, mg 173.2 mg nitrate/day) for seven days, and control group (n=14) was prohibited from high nitrate intake.
Results: During exercise, watercress group showed significant changes in the maximum values of Respiratory Exchange Ratio (RER) (p<0.05), End-Tidal O2 Fraction (FETO2) (p<0.05), and energy consumption from carbohydrate (p<0.01). Red radish group had a significant increase in the VCO2 (p<0.01), RER (p<0.01), VT (p<0.05), VCO2/kg (p<0.05), and energy consumption from carbohydrates (p<0.01). When all groups in the same workload were normalized by the subject’s body mass, watercress had a significant increase in the total expired CO2 (p<0.05), RER (p<0.05), FETO2 (p<0.05), and energy consumption from carbohydrates (p<0.05) compared with the control group. Similar comparison between red radish and control group revealed a significant increase during pre-test in the total CO2 production (p<0.05), VCO2 (p<0.05), RER (p<0.01), VT (p<0.05), and VCO2/kg (p<0.05).
Conclusion: Current results indicate higher carbon dioxide production in the experimental groups in the same workload. This might have a negative impact on the exercise performance. Further investigations with controlled exercise program will be necessary.

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Bailey SJ, Winyard P, Vanhatalo A, Blackwell JR,  DiMenna FJ, Wilkerson DP, Tarr J, Benjamin N, Jones AM. 2009. Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J Appl Physiol, 107: 1144-1155.

Bloomer RJ. 2010. Nitric oxide supplements for sports. Strength and Conditioning Journal, 32: 14-20.

Bloomer RJ,  Farney T,  Trepanowski J,  McCarthy C, Canale R. 2011. Effect of betaine supplementation on plasma nitrate/nitrite in exercise-trained men. J Int Soc Sports Nutr, 8: 5-12.

Bond H, Morton L, Braakhuis AJ. 2012. Dietary Nitrate Supplementation Improves Rowing Performance in Well-Trained Rowers. Int J Sport Nutr Exerc Metab, 22: 251-256.

Bruce RA,  Fisher LD,  Cooper MN, Gey GO. 1974. Separation of effects of cardiovascular disease and age on ventricular function with maximal exercise. Am J Cardiol, 34: 757-763.

Butler AR, Feelisch M. 2008. Therapeutic uses of inorganic nitrite and nitrate from the past to the future. Circulation, 117: 2151-2159.

Carlstrom M,  Larsen FJ, Nystrom T, Hezel M, Borniquel S, Weitzberg E, Lundberg JO. 2010. Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice. Proceedings of the National Academy of Sciences, 107: 17716-17720.

Dickstein K,  Barvik S,  Aarsland T, Snapinn S, Millerhagen J. 1990. Validation of a computerized technique for detection of the gas exchange anaerobic threshold in cardiac disease. Am J Cardiol, 66:1363-1367.

EFSA. 2008. Nitrate in vegetables: scientific opinion of the panel on contaminants in the food chain. European Food Safety Authority Journal, 689: 1-79.

Gladwin MT, Schechter AN, Kim-Shapiro DB, Patel RP, Hogg N, Shiva S, Cannon RO, Kelm M, Wink DA, Espey MG. 2005. The emerging biology of the nitrite anion. Nat Chem Biol, 1: 308-314.

Hord NG, Tang Y, Bryan NS. 2009. Food sources of nitrates and nitrites: the physiologic context for potential health benefits. Am J Clin Nutr, 90: 1-10.

Lansley KE, Winyard PG,  Fulford J,  Vanhatalo A, Bailey SJ,  Blackwell JR,  Dimenna FJ, Gilchrist M,  Benjamin N, Jones AM. 2011. Dietary nitrate supplementation reduces the O2 cost of walking and running: a placebo-controlled study. J Appl Physiol, 110: 591-600.

Larsen FJ,  Ekblom B,  Sahlin K, Lundberg JO, Weitzberg E. 2006. Effects of dietary nitrate on blood pressure in healthy volunteers. N Engl J Med, 355: 2792-2793.

Larsen FJ,  Schiffer TA,  Borniquel S,  Sahlin K, Ekblom B,  Lundberg JO, Weitzberg E. 2011. Dietary Inorganic Nitrate Improves Mitochondrial Efficiency in Humans. Cell Metab, 13: 149.

Larsen FJ, Weitzberg E, Lundberg JO, Ekblom B. 2010. Dietary nitrate reduces maximal oxygen consumption while maintaining work performance in maximal exercise. Free Radic Biol Med, 48: 342-347.

Lundberg JO, Weitzberg E, Gladwin MT. 2008. The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov, 7: 156-167.

Nair KS, Irving BA, Lanza IR. 2011. Can Dietary Nitrates Enhance the Efficiency of Mitochondria? Cell Metab, 13: 117-118.

Petersson J, Phillipson M,  Jansson EA, Patzak A, Lundberg JO, Holm L. 2007. Dietary nitrate increases gastric mucosal blood flow and mucosal defense. Am J Physiol Gastrointest Liver Physiol, 292: G718-G724.

Shiva S, Sack MN, Greer JJ, Duranski M,  Ringwood LA, Burwell L, Wang X,  MacArthur PH, Shoja A, Raghavachari N. 2007. Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer. J Exp Med, 204: 2089-2102.

Siahkouhian M, Meamarbashi A. 2013. Advanced methodological approach in determination of the heart rate deflection point: S.Dmax versus L.Dmax methods. J Sports Med Phys Fitness, 53: 27-33.

Tabatabaei M, Evaluation of NO3 concentration in leafy, fruit bearing and root vegetables of Tabriz of Iran, 8-10 November 2005, Proceedings of 4th Iranian Horticultural Science Congress, Mashhad, Iran.

van Faassen EE, Bahrami S,  Feelisch M,  Hogg N,  Kelm M,  Kim-Shapiro DB,  Kozlov AV,  Li H,  Lundberg JO, Mason R. 2009. Nitrite as regulator of hypoxic signaling in mammalian physiology. Med Res Rev, 29: 683-741.

Vanhatalo A, Bailey SJ,  Blackwell JR,  Dimenna FJ, Pavey TG,  Wilkerson DP,  Benjamin N, Winyard PG, Jones AM. 2010. Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise. Am J Physiol Regul Integr Comp Physiol, 299: R1121- 1131.

Williams MH 2012 Nitrates,Nitrites,Nitric Oxide and Exercise Performance, pp. 1-22 (Norfolk,VA, Old Dominion University).

Yeh MP,  Gardner RM,  Adams TD,  Yanowitz FG, Crapo RO. 1983. "Anaerobic threshold": problems of determination and validation. J Appl Physiol, 55: 1178-1186.