Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.)

Document Type: Original Research Article


1 Department of Laboratory Science, Babol-Branch, Islamic Azad University, Babol, I. R. Iran

2 Faculty of Basic Science, University of Mazandaran, Babolsar, I. R. Iran

3 Faculty of Chemistry, University of Mazandaran, Babolsar, I. R. Iran


Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE) have been performed in vitro.
Material and Methods: In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V) solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H2O2) by spectrophotometric method and chelating of ferrous ions by ferrozine reagent.
Results: HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE) revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract.
Conclusion: Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe+2chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers.


Main Subjects

De Tommasi N, Aquino R, De Simona F, Pizza C. 1992. Plant metabolites. new sesquiterpene and ionona glycosides from Eryobotrya japonica. J Nat Prod,
55: 1025-1032.

Dinis TCP, Madeira VMC, Almeida LM. 1994. Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys, 315: 161-169.

Ebrahimzadeh MA, Nabavi SM, Nabavi SF. 2009. Correlation between the in vitro iron chelating activity and poly phenol and flavonoid contents of some medicinal plants. Pakis J Biol Sci, 12: 934-938.

Ferreres F, Gomes D, Valentao P, Goncalves R, Pio R, Chagas EA, Seabra RM, Andrade PB. 2009. Improved loquat (Eriobotrya japonica Lindl.) cultivars: Variation of phenolics and antioxidative potential. Food Chem, 114: 1019-1027.

Fukumoto L, Mazza G. 2000. Asessing antioxidant and prooxidant activities of phenolic compounds. J Agri Food Chem, 48: 3597-3604.

Grazul M, Budzisz E. 2009. Biological activity of metal ions complexes of chromones, coumarins and flavones. Coord Chem Rev, 253: 2588-2598.

Hertog MC, Hollman PCH, Venema DP. 1992. Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. J Agri Food Chem, 40: 1591-1598.

Ito H, Kobayashi E, Takamatsu Y, Li SH, Hatano T, Sakagami H, et al. 2000. Polyphenols from Eriobotrya japonica and their cytotoxicity against human oral tumor cell lines. Chem Pharm Bull, 48: 687-693.

Jia Z, Tang M, Wu J. 1999. The determination of flavonoids content in mulberry and their scavenging effect on superoxide radicals. Food Chem, 64: 555-599.

Lu H, Li CWL, Wu RJL, Zhang HQ. 2009. Hypoglycemic effect of the total flavonoid fraction from Folium Eribotrya. Phytomedicine, 16: 967-971.

Peluso MR. 2006. Flavonoids attenuate cardiovascular disease, inhibit phosphodiesterase and modulate lipid homeostasis in adipose tissue and liver. Exp Biol Med, 231: 1287-1299.

Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10: 1003-1008.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods in Enzymology, 299: 152-178.

Vaughan JG, Geissler CA. 1997. The New Oxford Book of food plants. pp. 110-111, New York: Oxford University Press.

Yamaguchi T, Takamura H, Matoba T, Terao Y. 1998. HPLC method for evaluation of the free radical-scavenging activity of food by using 1,1-diphenil,-2-picrylhydrazil. Biosci Biotech Biochem, 62: 1201-1204.

Yen GC, Chen HY. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity, J Agri Food Chem, 43: 27-32.