Protective effect of Petroselinum crispum extract in abortion using prostadin-induced renal dysfunction in female rats

Document Type: Original Research Article

Authors

Department of Biology, Urmia University, Urmia, I.R.Iran

Abstract

Objective: Present study investigated the effects of parsley extract on pregnant rat kidneys which have undergone clinical abortion using prostaglandins. The renal protective effect of parsley extract was evaluated in pregnant rats which had an abortion. Parsley was used due to its antioxidant properties.
Materials and Methods:  Fifty-four female rats were divided in 9 groups of 6: control pregnant, two pregnant groups which received parsley extract and prostadin, two non-pregnant groups treated with parsley extract and prostadin, a group administered with both treatments, and three groups which received parsley extract in pre-implantation, implantation, and post-implantation periods of embryos. Ethanolic extract (5 mg/kg) was given daily to animals for 18 days of pregnancy period. Parameters such as malondialdehyde (MDA), total antioxidant statues (TAS), creatinine, and urea were measured using biochemical assays. Histopathologic studies were also done with Hematoxylin-Eosin staining method.
Results: After 18 days of treatment, significant differences were observed in serum creatinine, urea, and MDA and TAS levels. Kidney cross-sections showed edema in prostadin-treated rats while improvements in parsley + prostadin -treated rats were observed.
Conclusion: These results suggested that ethanolic extract of Petroselinum crispum reduced the dysfunction in rats kidney caused by prostadin-induced abortion and could have beneficial effect in reducing the progression of prostaglandin-induced edema.

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Alderson NL, Chachich ME, Frizzell N, Canning P, Metz TO et al. 2004. Effect of antioxidants and ACE inhibition on chemical modification of proteins and progression of nephropathy in streptozotocin diabetic rat. Diabetologia, 47: 1385-1395.

Al-Howiniry T, Al-Sohaibani M, El-Tahir K, Rafatullah S. 2003. Prevention of experimentally-induced gastric ulcers in rats by an ethanolic extract of "parsley", Petroselinum crispum. Am J Chim Med, 31: 699-711.

Ashraf H, Heidari R, Nejati V, Ilkhanipoor M. 2013. Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats. Avicenna J Phytomed, 3: 82-90

Darias V, Martin-Herrera D, Abdala S, Fuente D. 2001. Plants used in urinary pathologies in the Canary Islands. Pharm Biol, 39: 170-180.

Davey M.W, Bauw G, Montagu M.V. 1996. Analysis of ascorbate in plant tissue by high performance capillary zone electrophoresis. Anal Biochem, 239: 8–19.

Fejes S, Kery A, Blazovics A, Lugasi A, Lemberkovics E, Petri G, Szoke E. 1998. Investigation of the in vitroantioxidant effect of Petroselinum crispum (Mill.) Nym. Ex A.W. Hill. Acta Pharmacol Hung, 68: 150-156.

Fejes S.Z, Blazovics A, Lemberkovics E, Petri G, Szoke E, Kery A. 2000. Free radical scavenging and membrane protective effects of methanol extract from Anthriscus cerefolium L. (Hoffm) and Petroselinum crispum (Mill) Nym Ex A W Hill. Phyto Res, 14: 362-365.

Fiad S, El Hamidi M. 1993. Vitamin E and trace elements. Seifen Oele Fette Wasche, 119: 25-26.

Francis G.W, Isaksen M. 1989. Droplets counter current chromatography of the carotenoids of parsley Petroselinum crispum. Chromatographia, 27: 549–551.

Gadi D, Bnouham M, Aziz M, Ziyyat A, Legssyer A, Legrand Ch, Lafeve F.F, Mekhfi H. 2009. Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats. J Ethnopharmacol, 125: 170-174.

Giorgino F, Lavida L, Cavallo PP, Solnica B, Fuller J, et al. 2004. Factors associated with progression to macroalbuminuria in microalbuminuria type 1 patient: Eurodiab prospective and complications study. Diabetologia, 4: 1413-1418.

Kreyydieh S.I, Usta J, Kaouk I, Al-Sadi R. 2001. The mechanism underlying the laxative properties of parsley extract. Phytomed, 8: 382-388.

Mauer SM, Steffes MW, Brown DM.1981. The kidney in diabetes. Am J Med, 70:603-612.

Nawel M, Mohamed E, Amine D, Hocine A, Boufeldja T. 2011. Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed, 468-471.

Neuhaus-Carlisle K, Vierling W, Wagner H. 1993. Calcium-channel blocking activity of essential oils from Petroselinum crispum, Apium graveolens and isolated phenyl propane constituents. Pharm Pharmacol Lett, 3: 77-79.

Rosolowsky ET, Niewczas MA, Ficociello LH, Perkins BA, Warram JH, et al. 2008. Between hyperfiltration and impairment: demystifying early renal functional changes in diabetic nephropathy. Diabetes Res Clin Pract, 13: 46-53.

Sener GK, Sacan O, Yanardag R, Ayanoglu-Du Lger G.L. 2003. Effects of Parsley (Petroselinum crispum) on the Aorta and Heart of Stz Induced Diabetic Rats, P Foods Hum Nut 58: 1-7.

Sharma I, Dhaliwal L, Saha S, Sangwan S, Dhawan V. 2010. "Role of 8-iso-prostaglandin F2alpha and 25-hydroxycholesterol in the pathophysiology of endometriosis". Fert and Ster, 94: 6370.

Spraul MH, Nitz S, Drawert F. 1991. The chemical composition of parsley root and seed extracts. Chem Microbial Tech lebensm, 13: 179-182.

Stambe C, Atkins RC, Tesch GH, Kapoun AM, Hill PA, et al. 2003. Blockade of p38alpha MAPK ameliorates acute inflammatory renal injury in rat anti-GBM glomerulonephritis. J Am Soc Nephrol, 14:338-351.

Tyler VE. 1993. The honest herbalist. 3rd ed. New York, Pharmaceutical Products Press.

Yanardag R, Bolkent S, Tabakoglu-Ogluz A, Ozoy-Sacan O. 2003. Effects of Petroselinum crispum extract on pancreatic B cells and blood glucose of streptozotocin-induced diabetic rats. Biol Pharm Bull 26: 1206-1210.

Zheng G.Q, Kenney P.M, Zhang J, Lam L.K. 1992. Inhibition of benzo[a]pyrene-induced tumorigenesis by myristicin, a volatile aroma constituent of parsley leaf oil. Carcinogen, 13: 1921-1923.