Effects of saffron (Crocus sativus) petal ethanolic extract on hematology, antibody response, and spleen histology in rats

Document Type: Original Research Article


1 Department of Animal Sciences, Agriculture Faculty, Ferdowsi University of Mashhad, I. R. Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, I. R. Iran


Objective: Saffron petal is a by-product that contains flavonoids and anthocyanins. In order to study the effects of saffron petal extract (SPE) on blood parameters, immune system, and spleen histology, five treatments (n=6) were used in a completely randomized design.
Materials and Methods: The treatments were 0, 75, 150, 225, and 450 mg/kg body weight of SPE. The SPE was injected intraperitoneally to 30 rats (10-week old, weighing 225±15 g) for 14 days. Immunization was performed using 1×108 sheep red blood cells (SRBC) on days 0 and 7 subcutaneously in all treatment groups. On day 15, blood was collected from the heart of rats after anesthesia. One part of samples were poured in heparinized tubes for counting whole blood cells (CBC) and different white blood cells (WBC) and the other part was used to measure IgG using ELISA technique. The spleen was stained by hematoxylin- eosin for histological study. The data were statistically analyzed using ANOVA program and the means evaluation was done using Tukey’s test. Results are presented as mean±SD.
Results: Results showed no significant difference between treatments and control group regarding the amount of RBC, HGB, HCT, and PLT. The level of IgG at 75 mg/kg was significantly increased in comparison with other groups. No changes were observed in spleen histology.
Conclusion: The results indicate that use of SPE at dose of 75 mg/kg causes an increase in antibody response without any change in hematological parameters and spleen histology.


Main Subjects

Abdullaev FI, Espinosa Aguirre JJ. 2004. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer detect prev, 28: 426- 432.

Abdullaev FI. 2001. Cancer Chemopreventive and Tumoricidal Properties of Saffron (Crocus sativus L.). Exp Biol Med, 227: 20-25.

Abolhasani A, Bathaie SZ, Yavari I, Moosavi-Movahedi AA. 2005. Separation and purification of some components of Iraninan saffron. Asian J Chem, 2: 727- 729.

Agrawal SS, Khadase SC, Talele GS. 2010. Studies on immunomodulatory activity of capparis Zeylanica Leaf Extracts. Int J Pharm Sci Nonotechnol, 3: 887-892.

Akhondzadeh Basti A, Moshiri E, Noorbala AA, Jamshidi AH, Abbasi SH, Akhondzadeh S. 2007. Comparison of petal of Crocus sativus L. and fluoxetine in the treatment of depressed outpatients: A pilot double-blind randomized trial. Prog Neuropsychopharmacol Biol Psychiatry, 31: 439-442.

Assimopoulou AN, Sinakos Z, Papageorgiou VP. 2005. Radical Scavenging Activity of Crocus sativus L. Extract and its Bioactive Constituents. Phytother Res, 19: 996-1000.

Costantini D, Dell Omo G. 2006. Effects of T-cell-mediated immune response on avian oxidative stress. Comp Biochem Physiol A Mol Integr Physiol. 145: 137- 142.

Das I, Chakrabarty RN, Das S. 2004. Saffron can prevent chemically induced skin carcionogenesis in swiss albino mice. Asian Pac J cancer Prev, 5: 70-76.

Gil MI, kaber AA. 2002. Antioxidant capacities, phenolic compounds, carotenoids and vitamin C contents of nectarine, peach and plum cultivars from colifornia. J Agric food chem, 50: 7976-4982.

Goli SAH, Mokhtari F, Rahimmalek M. 2012. Phenolic compounds and antioxidant activity from saffron (crocus sativus) petal. J Agri  sci, 4: 10.

Goupy P, Abert Vian M, Chema F, Caris-Veyrat C. 2013. Identification and quantification of flavonols, anthocyanins and lutein diesters in tepals of Crocus sativus by ultra performance liquid chromatography coupled to diode array and ion trap mass spectrometry detections. Ind Crop Prod, 44: 496-510.

Horak P, Sats L, Zlmer M, Karu U, Zilmer K. 2007. Dodietary antioxidants alleviate the cost of immune activion? An experiment with greenfinches. Am Nat, 170: 625-635.

Hosseinzadeh H, Younesi HM. 2002. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol, 2: 7.

Hosseinzadeh H, Karimi GH, Niapoor M. 2004. Antidepressant effect of crocus sativus stigma extract and its constituents, crocin and safranal, in mice. Acta Hort. 650: 435-445.

Hosseinzadeh H, Motamedshariaty V, Hadizadeh F. 2007. Antidepressant effect of Kempferol, a constituent of saffran( Crocus sativus) petal, in mice and rats. Pharmacologyonline, 2: 367-370.

Hossinzadeh H, Behravan J, Ramazani M, Ajhakan KH. 2005. Evaluation effect of antitumor and cytotoxic stigma and petal of saffron. J pharmacol plant, 4.[In Persian].

Karimi GH, Hossnizadeh H, Khaleghpanah P. 2001. Study of antidepressent effect of queous and ethanolic extract of crocus sativus in mice. Iranian  J Basic Med Sic, 4: 11-15.

Karimi GR, Tayebi N, Hosseinzadeh H, Shirzad F. 2004. Evaluation of Subacute Toxicity of Aqueous Extract of Crocus sativus L. Stigma and Petal in Rats. Medicinal Plants, 3: 71.

Kianbakht S, Ghazavi A. 2011. Immunomodulatory Effects of Saffron: A Randomized Double‐Blind Placebo‐Controlled Clinical Trial. Phytother Res, 25: 1801–1805.

MC Manus JF. 1948. Histological and histochemical use of periodic acid. stain technology, 23: 99- 108.

Middleton EJ. 1998. Effect of plant flavonoids on immune and inflammatory cell function. Adv  Exp Med Bio, 439: 175-182.

Patwardhan B, Gautam M. 2005. Botanical immunodrags:scope and opportanities. Drug Discovery Today, 10: 495-502.

Plaeger SF. 2003. Clinical immunology and traditional herbal medicines. Clin Diagn Lab Immunol,10: 337-338.

Ranmadan A, Soliman G, Sawsan SM, Salwa MN, Rehab  FA. 2012. Evaluation of the safety and antioxidant activities of crocus sativus and propolis ethanolic extract. J Saudi chem soc, 16: 13-21

Sanchez Vioque R,  Rodriguez Conde MF, Reina Urena JV, Escolano Tercero MA, Herraiz Penalver D, Santana Meridas O. 2012. In vitro antioxidant and metal chelating properties of corm, petal and leaf from saffron (Crocus sativus L.). Ind Crop Prod, 39: 149-153.

Sarang B and Anjali P. 2011. Selective Th2 Upregulation by Crocus sativus :A Neutraceutical Spice. ECAM, 639862: 1-9.

Sheng L, Qian Z, Zheng S, Xi L. 2006. Mechanism of hypolipidemic effect of crocin in rats: crocin inhibits panceractic lipase. Eur J Pharmacol, 543:116-122.

Tajali F, Hemati Kakhki A, Khatamirad M, Garzani S, Garzani S. 2008. Study antioxidantion properties of saffron petal. 18 th National Congress on food Tecnology. Mashhad. I. R. Iran, 15-16.

Trease GE, Evans WC. 1983. Pharmacognosy. London, Bailliere Tindall press, 309- 706.

Vaibhav DA, Arun Kumar W. 2010. Immunomodulatory effect of alcoholic extract of terminalia chebula ripe fruits. J Pharm Sci Res, 2: 539-544.

Von Schant T, Bensch S, Grahn M, Hasselquist D, Wittzell H. 1999. Good geness, oxidative stress and condition - dependent sexual signal. PROC R SOC LOND B, 266: 1- 12.

Wieland HA, Michaelis M, Kischboum BJ, Rudolphi KA. 2005. Osteoarthritis - an untreatable disease? Nat Rev Drug Discov, 4: 331-44.

Xia Z, Wang G, Wan C, Liu T, Wang S, Wang B, Cheng R. 2010. Expression of NALP3 in the spleen of mice with protal hypertension. J Huazhong Univ Sci Technolog Med Sci, 30: 170-172.