Analgesic effect of the aqueous and ethanolic extracts of clove

Document Type: Original Research Article

Authors

1 Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

2 Department of Physiology, School of Medicine, Zabol University of Medical Sciences, Zabol, I. R. Iran

3 Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad I. R. Iran

Abstract

Objective: The beneficial effects of clove on toothache have been well documented. We have also previously shown the analgesic effects of clove essential oil. The present work was done to investigate the analgesic effects of the aqueous extract of clove using hot plate test. The possible role of opioid receptors in the analgesic effects of clove was also investigated using naloxone.
Materials and Methods: Ninety male mice were divided into nine groups: (1) Saline, (2-4) Aaqueous (Aq 50, Aq 100, and Aq 200) groups which were treated with 50, 100, and 200 mg/kg of aqueous extract of clove, respectively, (5-7) Ethanolic (Eth 50, Eth 100, and Eth 200) groups which were treated with 50, 100, and 200 mg/kg of ethanolic extract of clove, respectively, and (8-9) Aq 100- Naloxone and Aq 200- Naloxone which were pretreated with 4 mg/kg of naloxone before injection of 100 or 200 mg/kg of the aqueous extract. The hot plate test was performed as a base record 10 min before injection of drugs and consequently repeated every 10 minutes after the injection.
Results: The maximal percent effect (MPE) in the animal groups treated with 50, 100, and 200 mg/kg of aqueous extract was significantly higher than the control group. Pretreatment with naloxone reduced the analgesic effects of both 100 and 200 mg/kg of the aqueous extract. Administration of all three doses of the ethanloic extract also non-significantly increased the MPE.
Conclusion: The results of the present study showed that aqueous extract of clove has analgesic effect in mice demonstrated by hot plate test which is reversible by naloxone. The role of opioid system in the analgesic effect of clove might be suggested. However, more investigations are needed to elucidate the exact mechanism(s).

Keywords


Anderson WG, McKinley RS, Colavecchia M. 1997. The use of clove oil as an anesthetic for rainbow trout and its effects on swimming performance. N Am J Fish Manag, 17: 301-307.

Aoshima H, Hamamoto K. 1999. Potentiation of GABAA receptors expressed in Xenopus oocytes by perfume and phytoncid. Biosci Biotechnol Biochem, 63: 743-748.

Arung ET, Matsubara E, Kusuma IW, Sukaton E, Shimizu K, Kondo R. 2011. Inhibitory components from the buds of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells. Fitoterapia, 82: 198-202.

Avicenna A. 1988. In: Ghanoon dar Teb. Soroosh, Tehran, 244-251.

Baytop T. 1999. Therapy with medicinal plants in Turkey (Past and Present). No. 3255 (2nd ed., pp. 244-245). Istanbul: Publications of the Istanbul University.

Cai L, Wu CD. 1996. Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens. J Nat Prod, 59: 987-990.

Chaieb K, Hajlaoui H, Zmantar T, Kahla Nakbi AB, Rouabhia M, Mahdouani K, Bakhrouf A. 2007. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytother Res, 21: 501-506.

Cho JS, Kim TH, Lim JM, Song JH. 2008. Effects of eugenol on Na+ currents in rat dorsal root ganglion neurons. Brain Res, 1243: 53-62.

Dallmeier K, Carlini EA. 1981. Anesthetic, hypothermic, myorelaxant and anticonvulsant effects of synthetic eugenol derivatives and natural analogues. Pharmacol Pharmacol, 22: 113-127.

Damiani CEN, Rossoni LV, Vassallo DV. 2003. Vasorelaxant effects of eugenol on rat thoracic aorta. Vasc pharmacol, 40: 59-66.

Daniel AN, Sartoretto SM, Schmidt G, Caparroz-Assef SM, Bersani-Amado CA, Cuman R KN. 2009. Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models. Rev Bras Farmacogn, 19: 212-217.

Diaz MR, Sembrano JM. 1985. A comparative study of the efficacy of garlic and eugenol as palliative agents against dental pain of pulpal origin. J Philipp Dent Assoc, 35: 3-10.

Elwakeel HA, Moneim HA, Farid M, Gohar AA. 2007. Clove oil cream: a new effective treatment for chronic anal fissure. Colorectal Dis, 9: 549-552.

Feng J, Lipton JM. 1987. Eugenol: Antipyretic activity in rabbits. Neuropharmacol, 26: 1775-1778.

Ghelardini C, Galeotti N, Di Cesare Mannelli L, Mazzanti G, Bartolini A. 2001. Local anaesthetic activity of [beta]-caryophyllene. Il Farmaco, 56: 387-389.

Guénette SA, Ross A, Marier JF, Beaudry F, Vachon P. 2007. Pharmacokinetics of eugenol and its effects on thermal hypersensitivity in rats. Eur J pharmacol, 562: 60-67.

Halder S, Mehta AK, Mediratta PK, Sharma KK.2012. Acute effect of essential oil of Eugenia caryophyllata on cognition and pain in mice. Naunyn Schmiedebergs Arch Pharmacol,  385: 587-593.

Hosseini M, Asl MK, Rakhshandeh H. 2011. Analgesic effect of clove essential oil in mice. Avicenna J Phytomed, 1: 1-6.

Hosseini M, Sadeghnia HR, Salehabadi S, Alavi H, Gorji A. 2009. The effect of L-arginine and L-NAME on pentylenetetrazole induced seizures in ovariectomized rats, an in vivo study. Seizure, 18: 695-698.

Karami R, Hosseini M, Khodabandehloo F, Khatami L, Taiarani Z. 2011. Different effects of L-arginine on morphine tolerance in sham and ovariectomized female mice. J Zhejiang Univ Sci B, 12: 1016-1023.

Kim HM, Lee EH, Hong SH, Song HJ, Shin MK, Kim SH, Shin TY. 1998. Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats. J ethnopharmacol, 60: 125-131.

Kouidhi B, Zmantar T, Bakhrouf A. 2010. Anticariogenic and cytotoxic activity of clove essential oil (Eugenia caryophyllata) against a large number of oral pathogens. Ann Microbiol, 60: 599-604.

Kozam G. 1977. The effect of eugenol on nerve transmission. Oral Surg Oral Med Oral Pathol, 44: 799-805.

Kurian R, Arulmozhi DK, Veeranjaneyulu A, Bodhankar SL. 2006. Effect of eugenol on animal models of nociception. Indian J of Pharmacol, 38: 341-345.

Muller M, Pape HC, Speckmann EJ, Gorji A. 2006. Effect of eugenol on spreading depression and epileptiform discharges in rat neocortical and hippocampal tissues. Neurosci, 140: 743-751.

Murakami Y, Shoji M, Hanazawa S, Tanaka S, Fujisawa S. 2003. Preventive effect of bis-eugenol, a eugenol ortho dimer, on lipopolysaccharide-stimulated nuclear factor kappa B activation and inflammatory cytokine expression in macrophages. Biochem pharmacol, 66: 1061-1066.

Nishijima H, Uchida R, Kameyama K, Kawakami N, Ohkubo T. and Kitamura, K. 1999. Mechanisms mediating the vasorelaxing action of eugenol, a pungent oil, on rabbit arterial tissue. Jpn J Pharmacol, 79: 327-334.

Oztürk A, Ozbek H. 2005. The anti-inflammatory activity of Eugenia caryophyllata essential oil: an animal model of anti-inflammatory activity. Eur J Gen Med, 2: 159-163.

Park IS, Park SJ, Gil HW, Nam YK, Kim DS. 2011a. Anesthetic effects of clove oil and lidocaine-HCl on marine medaka (Oryzias dancena). Lab animal, 40: 45-51.

Park SH, Sim YB, Lee JK, Kim SM, Kang YJ, Jung JS, Suh H. W.2011b. The analgesic effects and mechanisms of orally administered eugenol. Arch Pharm Res, 34, 501-507.

Paula-Freire LI, Andersen ML, Molska GR, Kohn DO, Carlini EL.2012. Evaluation of the Antinociceptive Activity of Ocimum gratissimum L. (Lamiaceae) Essential Oil and Its isolated Active Principles in Mice. Phytother Res. Epub ahead of print.

Raghavenra H, Diwakr BT, Lokesh BR, Naidu KA.2006. Eugenol--The active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells. Prostaglandin Leukot Essent Fatty Acids, 74: 23-27.

Rakhshandeh H, Vahdati-Mashhadian N, Dolati K, Hosseini M. 2008. Antinociceptive effect of Rosa damascena in Mice. J Biol Sci, 8: 176-180.

Saeed S, Tariq P. 2008. In vitro antibacterial activity of clove against Gram negative bacteria. Pakistan J Botany, 40: 2157-2160.

Seidel. 2006. Initial and bulk extraction. Natural product isolation. 2nd ed. New Jersey: Humana Press; p. 27-46.

Sell AB, Carlini EA. 1976. Anesthetic action of methyleugenol and other eugenol derivatives. Pharmacology, 14, 367-77.

Sen P, Maiti PC, Puri S, Ray A, Audulov NA, Valdman AV. 1992. Mechanism of anti-stress activity of Ocimum sanctum Linn, eugenol and Tinospora malabarica in experimental animals. Indian J experiment boil, 30: 592-596.

Sepehri G , Shafeiee MN. 2006. Effect of Cuneiformis Nucleus Inactivation by Lidocaine Microinjection on the Analgesic Response of Morphine in Rats. Iran Biomed J, 10: 21-26.

Waterstrat PR. 1999. Induction and recovery from anesthesia in channel catfish Ictalurus punctatus fingerlings exposed to clove oil. J World Aquacult Soc, 30: 250-255.

Yang BH, Piao ZG, Kim YB, Lee CH, Lee JK, Park K, Kim JS, Oh SB. 2003. Activation of vanilloid receptor 1 (VR1) by eugenol. J Dent Res, 82: 781-785.

Yu  J, Hungju F. 1981. Studies on the essential oils of clove buds and clove leaves. Zhong Caoyao, 12: 339-342.

Zhang Y,  Chen Y. 1997. Isobiflorin, a chromone C-glucoside from cloves (Eugenia caryophyllata). Phytochemistry, 45: 401-403.

Zheng GQ, Kenney PM, Lam LKT. 1992. Sesquiterpenes from clove (Eugenia caryophyllata) as potential anticarcinogenic agents. J Nat Prod, 55: 999-1003.