Document Type: Short communication
Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Department of Anatomy, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Objective: In this study, the impact of arbutin was examined in a gentamicin (GM)-induced nephrotoxicity model.
Materials and Methods: Forty adult male Wistar rats were randomly assigned to five groups including control group; GM group, and three groups of GM+arbutin (25, 50 and 75 mg/kg). One day after the last injection of GM, creatinine, urea, carbonyl, thiobarbituric acid-reacting substance (TBARs), ferric reducing antioxidant power (FRAP) and 8-hydroxyguanosine levels were assessed in serum samples. Left and right kidneys were used for biochemical assays and histological evaluation, respectively.
Results: Our data showed that the FRAP level (p <0.05), urea (p <0.001), creatinine (p <0.001), and 8-hydroxyguanosine (p <0.001) levels of serum samples, were increased in GM-treated rats compared to the controls. The serum levels of TBARS (p <0.001) and carbonyl increased in serum and renal tissue (p <0.001) of GM-treated animals. Conversely, arbutin attenuated serum creatinine, urea and 8-hydroxyguanosine, and TBARS (p <0.001). Administration of arbutin significantly decreased carbonyl levels in serum and renal tissue samples (p <0.001). Furthermore, the levels of FRAP increased in the serum (p <0.01) and renal tissue samples (p <0.001) of arbutin-treated animals. Histological staining showed that arbutin significantly inhibits kidney damages.
Conclusion: Our data suggest that arbutin attenuates GM-induced nephrotoxicity through its free radicals-scavenging activity.