Neuroprotective effects of Tiliacora triandra leaf extract in a mice model of cerebral ischemia reperfusion

Document Type: Original Research Article

Authors

Animal Toxicology and Physiology Specialty Research Unit (ATPSRU), Physiology Division, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.

Abstract

Objective: The present study investigated possible neuroprotective effects of ethanolic extract of Tiliacora triandra leaf against cerebral ischemic-reperfusion injury in mice.
Materials and Methods: Forty male Institute of Cancer Research (ICR) mice were randomly divided into five groups: (1) Sham + 10% Tween 80, (2) bilateral common carotid artery occlusion (BCCAO) + 10% Tween 80, (3) BCCAO + T. triandra 300 mg/kg, (4) BCCAO + T. triandra 600 mg/kg and (5) BCCAO + quercetin 10 mg/kg. Cerebral ischemic-reperfusion (IR) was induced by 30 min of BCCAO followed by 45 min of reperfusion. After IR induction, total brain protein, calcium, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH), as well as brain infraction and histopathological changes in vulnerable brain areas, such as the cerebral cortex and hippocampus, were evaluated.
Results: The results showed that 2 weeks of pretreatment with T. triandra leaf extract at doses of 300 and 600 mg/kg significantly reduced calcium and MDA, but increased GSH and SOD and CAT activities. The extract significantly attenuated brain infarction and neuronal death in the cerebral cortex and hippocampus.
Conclusion: We demonstrated the neuroprotective effects of T. triandra leaf extract against cerebral IR injury in mice.

Keywords

Main Subjects


Beckman JS. 1991. The double-edged role of nitric oxide in brain function and superoxide-mediated injury. J Dev Physiol, 15: 53-59.

Boonsong P, Laohakunjit N, Kerdchoechuen O. 2009. Identification of polyphenolic compounds and colorants from Tiliacora triandra (Diels) Leaves. Agricultural Sci J, 40: 13-16.

Guo C, Tong L, Xi M, Yang H, Dong H, Wen A. 2012. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol, 144: 768-774.

Hadwan MH, Abed HN. 2016. Data supporting the spectrophotometric method for the estimation of catalase activity. Data in Brief, 6: 194-199.

Ingkaninan K, Temkitthawon P, Chuenchom K, Yuyaem T, Thongnoi W. 2003. Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies. J Ethnopharmacol, 89: 261-264.

Kaewpiboon C, Winayanuwattikun P, Yongvanich T, Phuwapraisirisan P, Assavalapsakul W. 2014. Effect of three fatty acids from the leaf extract of Tiliacora triandra on P-glycoprotein function in multidrug-resistant A549RT-eto cell line. Pharmacogn Mag, 10: S549-S556.

Leker RR, Shohami E. 2002. Cerebral ischemia and trauma different etiologies yet similar mechanisms: neuroprotective opportunities. Brain Res Rev, 39: 55-73.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem, 193: 265-275.

Makarov VG, Makarova MN, Selezneva AI. 2005. Studying the mechanism of antioxidant effect of vitamins and flavonoids. Vopr Pitan, 74: 10-13.

Mansoorali KP, Prakash T, Kotresha D, Prabhu K, Rama Rao N. 2012. Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats. Phytomedicine, 19: 1108-1116.

Mira L, Fernandez MT, Santos M, Rocha R, Florencio MH, Jennings KR. 2002. Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. Free Radic Res, 36: 1199-1208.

Pachaly P, Khosravian H. 1988. Tilitriandrin: a new bisbenzylisoquinoline alkaloid from tiliacora triandra. Planta Med, 54: 516-519.

Paris RR, Sasorith SK. 1967. On the alkaloids of 2 Menispermaceae from Laos: Cyclea barbata (Wall.) Miers and Tiliacora triandra (Roxb.) Diels. Ann Pharm Fr, 25: 627-633.

Paxinos G, Franklin K. 2001. The Mouse Brain in Stereotaxic Coordinates (3rd Ed), pp. 197-215, Academic Press.

Pedrielli P, Pedulli GF, Skibsted LH. 2001. Antioxidant mechanism of flavonoids. Solvent effect on rate constant for chain-breaking reaction of quercetin and epicatechin in autoxidation of methyl linoleate. J Agric Food Chem, 49:3034-3040.

Phadungkit M, Somdee T, Kangsadalampai K. 2012. Phytocheical screening, antioxidant and antimutagenic activities of selected Thai edible plant extracts. J Med Plants Res, 6:662-666.

Phillis JW, O'Regan MH, Donard SD. 2002. Energy utilization in the ischemic/reperfused brain. International Review of Neurobiology, pp. 377-414, Academic Press.

Phunchango N, Wattanathorn J, Chaisiwamongkol K. 2015. Tiliacora triandra, an anti-intoxication plant, improves memory impairment, neurodegeneration, cholinergic function, and OCidative stress in hippocampus of ethanol dependence rats. Oxid Med Cell Longev, 2015:1-9.

Raghavendra M, Rituparna M, Shafalika K, Anshuman T, Sumit M, SA. 2009. Role of Centella asiatica on cerebral post-ischemic reperfusion and long-term hypoperfusion in rats. Int J Green Pharm, 3:88-96.

Saiin C, Markmee S. 2003. Isolation of anti-malarial active compound from Yanang (Tiliacor triandra Diels). Kasetsart J Nat Sci, 37:47-51.

Sakamula R, Thong-Asa W. 2018. Neuroprotective effect of p-coumaric acid in mice with cerebral ischemia reperfusion injuries. Metab Brain Dis, 33: 765-773.

Singthong J, Oonsivilai R, Oonmetta-Aree J, Ningsanond S. 2014. Bioactive compounds and encapsulation of Yanang (Tiliacora triandra) leaves. Afr J Tradit Complement Altern Med, 11: 76-84.

Sireeratawong S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N, Jaijoy K. 2008. Acute and subchronic toxicity study of the water extract from Tiliacora triandra (Colebr.) Diels in rats. Songklanakarin J Sci Technol, 30: 611-619.

Spare PD. 1964. A Stable murexide reagent for the estimation of calcium in micro quantities of serum. Clin Chem, 10: 726-729.

Sureram S, Senadeera SPD, Hongmanee P, Mahidol C, Ruchirawat S, Kittakoop P. 2012. Antimycobacterial activity of bisbenzylisoquinoline alkaloids from Tiliacora triandra against multidrug-resistant isolates of Mycobacterium tuberculosis. Bioorg Med Chem Lett, 22: 2902-2905.

Singthong J, Oonsivilai R, Oonmetta-Aree J, Ningsanond S. 2014. Bioactive compounds and encapsulation of Yanang (Tiliacora triandra) leaves. Afr J Tradit Complement Altern Med, 11: 76-84.

Sireeratawong S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N. 2008. Acute and subchronic toxicity study of the water extract from Tiliacora triandra (Colebr.) Diels in rats. Songklanakarin J Sci Technol, 30: 611-9.

Spare PD. 1964. A Stable murexide reagent for the estimation of calcium in micro quantities of serum. Clin Chem, 10: 726-729.

Sureram S, Senadeera SPD, Hongmanee P, Mahidol C, Ruchirawat S, Kittakoop P. 2012. Antimycobacterial activity of bisbenzylisoquinoline alkaloids from Tiliacora triandra against multidrug-resistant isolates of Mycobacterium tuberculosis. Bioorg Med Chem Lett, 22: 2902-2905.

Thong-asa W, Tilokskulchai K. 2014. Neuronal damage of the dorsal hippocampus induced by long-term right common carotid artery occlusion in rats. Iran J Basic Med Sci, 17: 220-226.

Thong-asa W, Tumkiratiwong P, Bullangpoti V, Kongnirundonsuk K, Tilokskulchai K. 2017. Tiliacora triandra (Colebr.) Diels leaf extract enhances spatial learning and learning flexibility, and prevents dentate gyrus neuronal damage induced by cerebral ischemia/reperfusion injury in mice. Avicenna J Phytomed, 7: 389-400.

Vaghef L, Bafandeh Gharamaleki H. 2017. Effects of physical activity and Ginkgo Biloba on cognitive function and oxidative stress modulation in ischemic rats. Int J Angiol, 26: 158-164.

Wakita H, Tomimoto H, Akiguchi I, Matsuo A, Lin JX, Ihara M. 2002. Axonal damage and demyelination in the white matter after chronic cerebral hypoperfusion in the rat. Brain Res, 924: 63-70.

White BC, Sullivan JM, DeGracia DJ, O' Neil BJ, Neumar RW, Grossman LI. 200. Brain ischemia and reperfusion: molecular mechanisms of neuronal injury. J Neurol Sci, 179: 1-33.