Utilization of a chicken embryo membrane model for evaluation of embryonic vascular toxicity of Dorema ammoniacum

Document Type: Original Research Article

Authors

1 Department of Clinical Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

2 Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

4 Executive Secretary of Medical Ethics Committee, Vice-Chancellery of Research and Technology, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Objective: Extensive research has been done to assess the efficacy of herbs for treating different disorders. Dorema ammoniacum (D. ammoniacum) is used in folk medicines for various goals. The application of herbs in medicine is accompanied by harmful effects. Chick embryo is considered a suitable model for assessing drugs toxicity. The present study aimed to evaluate the changes in vasculature in chick’s extra-embryonic membrane following D. ammoniacum treatment. Alterations in molecular pathways associated with early embryonic angiogenesis such as vascular endothelial growth factor A (VEGF-A) were also evaluated.
Materials and Methods: Fertile chicken (Ross 308) eggs were allocated into three similar groups; sham, control and D. ammoniacum groups; in D. ammoniacum group, eggs were inoculated with plant’s extract at doses of 50 or 100 mg per kg egg-weight.
Results: Analysis of the extra-embryonic membrane vasculature revealed that D. ammoniacum extract decreases some vascular parameters such as vessels area, total vessels length, vascular branch and increases lacunarity. This herb’s vascular toxicity was in a dose-dependent manner. Down-regulation of the expression of VEGF-A was also seen in the extract-treated extra-embryonic membrane.
Conclusion: Vascular toxicity of D. ammoniacum was confirmed by data presented in this paper. We conclude that alteration of vascular parameters and gene expression might finally lead to embryo malformation due to D. ammoniacum consumption. Therefore, the use of this herb must be limited during the fetal growth period especially at doses higher than 50 mg per kg.

Keywords

Main Subjects


Amiri MS, Joharchi MR. 2013. Ethnobotanical investigation of traditional medicinal plants commercialized in the markets of Mashhad, Iran. Avicenna J Phytomed, 3: 254-271.

Amiri MS, Joharchi MR. 2016. Ethnobotanical knowledge of Apiaceae family in Iran: A review. Avicenna J Phytomed, 6: 621-635.

Araghi A, Seifi S, Sayrafi R, Sadighara P. 2016. Safety assessment of rice bran oil in a chicken embryo model. Avicenna J Phytomed, 6: 351-356.

Blacher S, Devy L, Hlushchuk R, Larger E, Lamandé N, Burri P, Corvol P, Djonov V, Foidart JM, Noël A. 2011. Quantification of angiogenesis in the chicken chorioallantoic membrane (CAM). Image Anal Stereol, 24: 169-180.

Borba FK, Felix GL, Costa EV, Silva L, Dias PF, Albuquerque R. 2016. Fractal analysis of extra-embryonic vessels of chick embryos under the effect of glucosamine and chondroitin sulfates. Microvasc Res, 105: 114-118.

Bulant C, Blanco P, Müller L, Scharfstein J, Svensjö E. 2017. Computer-aided quantification of microvascular networks: Application to alterations due to pathological angiogenesis in the hamster. Microvasc Res, 112: 53-64.

Chaiworapongsa T, Romero R, Whitten A, Korzeniewski S, Chaemsaithong P, Hernandez-Andrade E, Yeo L, Hassan S. 2016. The use of angiogenic biomarkers in maternal blood to identify which SGA fetuses will require a preterm delivery and mothers who will develop pre-eclampsia. J Matern Fetal Neonatal Med, 29: 1214-1228.

Chappell L, David A. 2016. Improving the pipeline for developing and testing pharmacological treatments in pregnancy. PLoS Med, 13: e1002161.

Demir R, Seval Y, Huppertz B. 2007. Vasculogenesis and angiogenesis in the early human placenta. Acta Histochem, 109: 257-265.

Dunnick J, Nyska A. 2013. The toxicity and pathology of selected dietary herbal medicines. Toxicol Pathol, 41: 374-386.

Eskandani M, Dadizadeh E, Hamishehkar H, Nazemiyeh H, Barar J. 2014. Geno/cytotoxicty and apoptotic properties of phenolic compounds from the seeds of dorema glabrum fisch. CA. Bioimpacts, 4: 191-198.

Feng Y, Yang H. 2017. Metformin–a potentially effective drug for gestational diabetes mellitus: a systematic review and meta-analysis. J Matern Fetal Neonatal Med, 30: 1874-1881.

Ferrara N, Adamis A. 2016. Ten years of anti-vascular endothelial growth factor therapy. Nat Rev Drug Discov, 15: 385-404.

Gheorghescu AK, Tywoniuk B, Duess J, Buchete NV, Thompson J. 2015. Exposure of chick embryos to cadmium changes the extra-embryonic vascular branching pattern and alters expression of VEGF-A and VEGF-R2. Toxicol Appl Pharmacol, 289: 79-88.

Gonzalez-Crussi F. 1971. Vasculogenesis in the chick embryo. An ultrastructural study. Am J Anat, 130: 441-459.

Groenendijk B, Hierck B, Vrolijk J, Baiker M, Pourquie M, Gittenberger A, Poelmann R. 2005. Changes in shear stress–related gene expression after experimentally altered venous return in the chicken embryo. Circ Res, 96: 1291-1298.

Hamburger V, Hamilton H. 1951. A series of normal stages in the development of the chick embryo. J Morphol, 88: 49-92.

Izzo A, Hoon‐Kim S, Radhakrishnan R, Williamson E. 2016. A critical approach to evaluating clinical efficacy, adverse events and drug interactions of herbal remedies. Phytother Res, 30: 691-700.

Kwan L, Mok M, Lam MF. 2014. Acute drug toxicity related to drinking herbal tea in a kidney transplant recipient. Ren Fail, 36: 309-312.

Liebner S, Czupalla C, Wolburg H. 2011. Current concepts of blood-brain barrier development. Int J Dev Biol, 55: 467-476.

Magnaudeix A, Usseglio J, Lasgorceix M, Lalloue F, Damia C, Brie J, Pascaud-Mathieu P, Champion E. 2016. Quantitative analysis of vascular colonisation and angio-conduction in porous silicon-substituted hydroxyapatite with various pore shapes in a chick chorioallantoic membrane (CAM) model. Acta Biomate, 38: 179-189.

Mahdavi N, Joukar S, Najafipour H, Asadi-Shekaari M. 2016. The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil. Pharm Biol, 54: 509-515.

Majidian-Eydgahi S, Baharara J, Zafar-Balanezhad S, Asadi-Samani M. 2015. The synergic effect of glycyrrhizic acid and low frequency electromagnetic field on angiogenesis in chick chorioallantoic membrane. Avicenna J Phytomed, 5: 174-181.

McKay T, Gedeon D, Vickerman M, Hylton A, Ribita D, Olar H, Kaiser P, Parsons-Wingerter P. 2008. Selective inhibition of angiogenesis in small blood vessels and decrease in vessel diameter throughout the vascular tree by triamcinolone acetonide. Invest Ophthalmol Vis Sci, 49: 1184-1190.

Motevalian M, Mehrzadi S, Ahadi S, Shojaii A. 2017. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors. Res Pharm Sci, 12: 53-59.

Nowak-Sliwinska P, Segura T, Iruela-Arispe L. 2014. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis, 17: 779-804.

Oosterbaan A, Steegers E, Ursem N. 2012. The effects of homocysteine and folic acid on angiogenesis and VEGF expression during chicken vascular development. Microvasc Res, 83: 98-104.

Paparozzi E. 2005. Plant Resins-Chemistry, Evolution Ecology and Ethnobotany. HortScience, 40: 508-508.

Paradkar P, Dandekar S, Joshi J, Amonkar A, Vaidya A. 2017. Assessment of In vitro-In vivo Antimigratory and Anti-angiogenic Activity of Curcuma longa Linn. and Tinospora cordifolia Willd. Extracts in Cervical Cancer. Int J Pharm Sci Rev Res, 42: 87-93.

Popova S, Lange S, Shield K, Mihic A, Chudley A, Mukherjee R, Bekmuradov D, Rehm J. 2016. Comorbidity of fetal alcohol spectrum disorder: a systematic review and meta-analysis. Lancet, 387: 978-987.

Resnick N, Einav S, Chen-Konak L, Zilberman M, Yahav H, Shay-Salit A. 2003. Hemodynamic forces as a stimulus for arteriogenesis. Endothelium, 10: 197-206.

Ribatti D. 2017. The chick embryo chorioallantoic membrane (CAM) assay. Reprod Toxicol, 70: 97-101.

Rouhi-Boroujeni H, Heidarian E, Rouhi-Boroujeni H, Deris F, Rafieian-Kopaei M. 2017. Medicinal Plants with multiple effects on cardiovascular diseases: a systematic review. Curr Pharm Des, 23: 999-1015.

Seidlitz E, Korbie D, Marien L, Richardson M, Singh G. 2004. Quantification of anti-angiogenesis using the capillaries of the chick chorioallantoic membrane demonstrates that the effect of human angiostatin is age-dependent. Microvasc Res, 67: 105-116.

Shibuya M. 2013. Vascular endothelial growth factor and its receptor system: physiological functions in angiogenesis and pathological roles in various diseases. J Biochem, 153: 13-19.

Somani S, Modi K, Majumdar A, Sadarani B. 2015. Phytochemicals and their potential usefulness in inflammatory bowel disease. Phytother Res, 29: 339-350.

Sreekanth P, Narayana K, Shridhar N, Bhat A. 2006. Toxicity studies of Calycopteris floribunda Lam. in calf, rabbit and rat. J Ethnopharmacol, 107: 229-233.

Thompson J, Bannigan J. 2007. Omphalocele induction in the chick embryo by administration of cadmium. J Pediatr Surg, 42: 1703-1709.

Wrigley A, Hughes J, Sheehan M, Wilkinson S, Hunter D. 2010. European textbook on ethics in research.

Yousefzadi M, Heidari M, Akbarpour M, Mirjalili MH, Zeinali A, Parsa M. 2011. In vitro cytotoxic activity of the essential oil of Dorema ammoniacum D. Don. Middle East J Sci Res, 7: 511-514.

Yousefzadi M, Mirjalili MH, Alnajar N, Zeinali A, Parsa M. 2011. Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran. J Serb Chem Soc, 76: 857-863.